scholarly journals Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.)

2020 ◽  
Vol 202 (10) ◽  
pp. 2727-2738 ◽  
Author(s):  
Urszula Perlińska-Lenart ◽  
Sebastian Piłsyk ◽  
Elżbieta Gryz ◽  
Jadwiga Turło ◽  
Dorota Hilszczańska ◽  
...  
Keyword(s):  
Fungal Species ◽  
Fruiting Bodies ◽  
Tuber Aestivum ◽  
Its1 Region ◽  
Cultivable Bacteria ◽  
The Family ◽  
Burgundy Truffle ◽  
Identification Of Bacteria ◽  
Bacteria And Fungi ◽  
Different Strains

Abstract Tuber species may be regarded as complex microhabitats hosting diverse microorganisms inside their fruiting bodies. Here, we investigated the structure of microbial communities inhabiting the gleba of wild growing (in stands) T. aestivum, using Illumina sequencing and culture-based methods. The two methods used in combination allowed to extract more information on complex microbiota of Tuber aestivum gleba. Analysis of the V3–V4 region of 16S rDNA identified nine phyla of bacteria present in the gleba of T. aestivum ascomata, mostly Proteobacteria from the family Bradyrhizobiaceae. Our results ideally match the earlier data for other Tuber species where the family Bradyrhizobiaceae was the most represented. The ITS1 region of fungal rDNA represented six alien fungal species belonging to three phyla. To complement the metagenomic analysis, cultivable fungi and bacteria were obtained from the gleba of the same T. aestivum fruiting bodies. The identified fungi mostly belong to the phylum Basidiomycota and same to Ascomycota. Analysis of cultivable bacteria revealed that all the specimens were colonized by different strains of Bacillus. Fungal community inhabiting T. aestivum fruiting bodies was never shown before.

scholarly journals Ectomycorrhizal communities in a Tuber aestivum Vittad. orchard in Poland

2016 ◽  
Vol 11 (1) ◽  
pp. 348-357 ◽  
Author(s):  
Dorota Hilszczańska ◽  
Hanna Szmidla ◽  
Jakub Horak ◽  
Aleksandra Rosa-Gruszecka
Keyword(s):  
Rural Communities ◽  
Host Species ◽  
Fruiting Bodies ◽  
Tuber Aestivum ◽  
Conventional Agriculture ◽  
Truffle Cultivation ◽  
Host Trees ◽  
Rural Economies ◽  
Burgundy Truffle ◽  
Ectomycorrhizal Communities

AbstractCultivation of the Burgundy truffle, Tuber aestivum Vittad., has become a new agricultural alternative in Poland. For rural economies, the concept of landscaping is often considerably more beneficial than conventional agriculture and promotes reforestation, as well as land-use stability. Considering examples from France, Italy, Hungary and Spain, truffle cultivation stimulates economic and social development of small, rural communities. Because there is no long tradition of truffle orchards in Poland, knowledge regarding the environmental factors regulating the formation of fruiting bodies of T. aestivum is limited. Thus, knowledge concerning ectomycorrhizal communities of T. aestivum host species is crucial to ensuring successful Burgundy truffle production. We investigated the persistence of T. aestivum ectomycorrhizae on roots of hazel (Corylus avellana L.) and oak (Quercus robur L.) and checked the host-species influence on community structure of ectomycorrhizal fungi. The study was conducted in an experimental plantation located in eastern Poland and established in 2008. We demonstrated that the number of fungal taxa was not significantly different between oak and hazel. However, the species composition differed between these two host trees. During the three-year study, we observed that species richness did not increase with the age of the plantation.

scholarly journals Fungi inhabiting the aboveground organs of downy willow (Salix lapponum L.) and swamp willow (Salix myrtilloides L.)

2019 ◽  
Vol 54 (2) ◽  
Author(s):  
Ewa Dorota Zalewska ◽  
Magdalena Pogorzelec ◽  
Ewa Dorota Król ◽  
Artur Serafin
Keyword(s):  
Plant Pathogens ◽  
Fungal Species ◽  
Peat Bogs ◽  
Lake District ◽  
Natural Habitats ◽  
Disease Symptoms ◽  
Cytospora Chrysosperma ◽  
The Family ◽  
Bacteria And Fungi ◽  
Salix Lapponum

The species of downy willow (<em>Salix lapponum</em>) and swamp willow (<em>Salix myrtilloides</em>) are extremely rare relicts among all members of the genus <em>Salix</em>. In Poland, they occur only in the eastern part of the Karkonosze Mountains, Masurian Lake District, and Polesie Lubelskie area. A huge threat to both species is the drainage of peat bogs and their excessive exploitation as well as diseases caused by bacteria and fungi. The present study aimed identify fungal species colonizing the aboveground organs of plants with disease symptoms and to indicate which species may pose a threat towards <em>S. lapponum</em> and <em>S. myrtilloides</em> growing under natural conditions. This research was conducted in Polesie National Park as a part of a research project by the Ministry of Science and Higher Education. Plant health assessments were carried out in natural habitats, while aboveground organs with disease symptoms were collected for laboratory mycological analysis. <em>Colletotrichum salicis</em> (teleomorph <em>Glomerella salicis</em>), <em>Coniothyrium concentricum</em>, <em>Cylindrosporium salicinum</em>, <em>Phomopsis</em> spp., <em>Truncatella angustata</em>, and <em>Cytospora chrysosperma</em> (teleomorph <em>Valsa sordida</em>) were the fungal species most frequently isolated from leaves and shoots of both willow species. The above-mentioned fungi are dangerous plant pathogens from the family Salicaceae. There were no obligatory parasites or monophagous pathogens on the aboveground organs of downy or swamp willow. Obligatory parasites may indicate a markedly low population of host plants in Polish conditions.

scholarly journals Ectomycorrhiza resilience and recovery to extreme flood events in Tuber aestivum and Quercus robur

Mycorrhiza ◽  
2021 ◽  
Author(s):  
P. W. Thomas
Keyword(s):  
Climate Change ◽  
Seedling Growth ◽  
Quercus Robur ◽  
Root Systems ◽  
Fruiting Bodies ◽  
Flood Events ◽  
Tuber Aestivum ◽  
Soil Zone ◽  
Extreme Flood ◽  
The Impact

AbstractVery little is known about the impact of flooding and ground saturation on ectomycorrhizal fungi (EcM) and increasing flood events are expected with predicted climate change. To explore this, seedlings inoculated with the EcM species Tuber aestivum were exposed to a range of flood durations. Oak seedlings inoculated with T. aestivum were submerged for between 7 and 65 days. After a minimum of 114-day recovery, seedling growth measurements were recorded, and root systems were destructively sampled to measure the number of existing mycorrhizae in different zones. Number of mycorrhizae did not display correlation with seedling growth measurements. Seven days of submersion resulted in a significant reduction in mycorrhizae numbers and numbers reduced most drastically in the upper zones. Increases in duration of submersion further impacted mycorrhizae numbers in the lowest soil zone only. T. aestivum mycorrhizae can survive flood durations of at least 65 days. After flooding, mycorrhizae occur in higher numbers in the lowest soil zone, suggesting a mix of resilience and recovery. The results will aid in furthering our understanding of EcM but also may aid in conservation initiatives as well as providing insight for those whose livelihoods revolve around the collection of EcM fruiting bodies or cropping of the plant partners.

scholarly journals Synthesis and Evaluation of Schiff Bases of 4-Amino-5-(chlorine substituted phenyl)-4H-1,2,4-triazole-3-thione as Antimicrobial Agents

2020 ◽  
Vol 41 (1) ◽  
pp. 26-35
Author(s):  
Kishor Devkota ◽  
Govinda Pathak ◽  
Bhushan Shakya
Keyword(s):  
Schiff Base ◽  
Schiff Bases ◽  
Antimicrobial Agents ◽  
Triazole Ring ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Spectral Techniques ◽  
Schiff Base Derivatives ◽  
Bacteria And Fungi ◽  
Different Strains

Triazole ring system has attracted a continuously growing interest of synthetic organic chemists and those dealing with the medicinal compounds due to its versatile potential to interact with biological systems. Schiff bases are also considered as one of the most biologically active compounds.  The aim of the present study was to synthesize new Schiff bases bearing triazole nucleus and to assess their antimicrobial activities. Four new Schiff base derivatives of 1,2,4-triazole-3-thione were synthesized by combining two different pharmacophores viz. triazole nucleus and Schiff base moiety and were characterized by spectral techniques (UV, FT-IR, and NMR). The Schiff bases were evaluated for antibacterial (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and antifungal (Candida albicans) activities. The synthesized compounds exhibited good to moderate activities against different strains of bacteria and fungi tested.

scholarly journals Multigene phylogeny and morphology reveal Phaeobotryon rhois sp. nov. (Botryosphaeriales, Ascomycota)

Phytotaxa ◽  
2015 ◽  
Vol 205 (2) ◽  
pp. 90 ◽  
Author(s):  
XIN-LEI FAN ◽  
KEVIN D. HYDE ◽  
JIAN-KUI LIU ◽  
YING-MEI LIANG ◽  
CHENG-MING TIAN
Keyword(s):  
New Species ◽  
Distinct Species ◽  
Similar Species ◽  
Multigene Phylogeny ◽  
Fruiting Bodies ◽  
Canker Disease ◽  
Multigene Analysis ◽  
The Family ◽  
Rhus Typhina ◽  
A New Species

The family Botryosphaeriaceae encompasses important plant-associated pathogens, endophytes and saprobes with a wide geographical and host distribution. Two dark-spored botryosphaeriaceous taxa associated with Rhus typhina dieback and canker disease were collected from Ningxia Province, in northwestern China. Morphology and multigene analysis (ITS, LSU and EF-1α) clearly distinguished this clade as a distinct species in the genus. Phaeobotryon rhois is introduced and illustrated as a new species in this paper. The species is characterized by its globose, unilocular fruiting bodies and small, brown, 1-septate conidia. It can be distinguished from the similar species P. cercidis, P. cupressi, P. mamane and P. quercicola based on host association and conidial size and colour.

The mycobiome of the oral cavity in healthy dogs and dogs with periodontal disease

2022 ◽  
pp. 1-8
Author(s):  
Brook A. Niemiec ◽  
Jerzy Gawor ◽  
Shuiquan Tang ◽  
Aishani Prem ◽  
Janina A. Krumbeck
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Dna Sequencing ◽  
Fungal Species ◽  
Next Generation ◽  
Data Set ◽  
Next Generation Dna Sequencing ◽  
Sequencing Platform ◽  
The Family ◽  
Healthy Dogs

Abstract OBJECTIVE To investigate the mycobiome of the oral cavity in healthy dogs and dogs with various stages of periodontal disease. ANIMALS 51 dogs without periodontal disease (n = 12) or with mild (10), moderate (19), or severe (10) periodontal disease. PROCEDURES The whole maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the internal transcribed spacer 2 region with a commercial sequencing platform. RESULTS Fungi were detected in all samples, with a total of 320 fungal species from 135 families detected in the data set. No single fungal species was found in all samples. The 3 most frequently found fungal species were Cladosporium sp (46/51 samples), Malassezia restricta (44/51 samples), and Malassezia arunalokei (36/51 samples). Certain fungi, specifically those of the family Didymellaceae, the family Irpicaceae, and the order Pleosporales, were significantly associated with different stages of periodontitis. Mycobial analysis indicated that Cladosporium sp could be considered part of the core oral cavity mycobiome. CONCLUSIONS AND CLINICAL RELEVANCE Results highlighted that fungi are present in the oral cavity of dogs and are characterized by substantial species diversity, with different fungal communities associated with various stages of periodontal disease. The next-generation DNA sequencing used in the present study revealed substantially more species of fungi than previous culture-based studies.

A mushroom-inducing DNA sequence isolated from the Basidiomycete, Schizophyllum commune.

Genetics ◽  
1991 ◽  
Vol 129 (3) ◽  
pp. 707-716 ◽  
Author(s):  
J S Horton ◽  
C A Raper
Keyword(s):  
Dna Sequence ◽  
Dna Sequences ◽  
De Novo ◽  
Schizophyllum Commune ◽  
Sequence Divergence ◽  
Fruiting Bodies ◽  
Genomic Dnas ◽  
Different Strains ◽  
Mating Interaction ◽  
Normal Requirement

Abstract A DNA sequence capable of inducing the de novo development of fruiting bodies (mushrooms) when integrated into the genome of unmated, nonfruiting strains of the Basidiomycete Schizophyllum commune has been isolated and partially characterized. This sequence, designated FRT1, overrides the normal requirement of a mating interaction for fruiting in this organism. It has been shown to integrate stably in different chromosome locations and appears to be trans-acting. It also enhances the normal process of fruiting that occurs after mating. Additional DNA sequences with similarity to FRT1 were detected within the genome of the strain of origin by hybridization of labeled FRT1 DNA to blots of digested genomic DNAs. FRT1 and the genomic sequences similar to it were shown to be genetically linked. Southern hybridization experiments suggested sequence divergence at the FRT1 locus between different strains of S. commune. A testable model for how FRT1 may act as a key element in the pathway for the differentiation of fruiting bodies is presented as a working hypothesis for further investigation.

scholarly journals Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
Alba Boix-Amorós ◽  
Fernando Puente-Sánchez ◽  
Elloise du Toit ◽  
Kaisa M. Linderborg ◽  
Yumei Zhang ◽  
...  
Keyword(s):  
Breast Milk ◽  
Potential Role ◽  
Mode Of Delivery ◽  
Geographical Location ◽  
Geographic Location ◽  
Fungal Species ◽  
Human Breast Milk ◽  
Human Breast ◽  
Its1 Region ◽  
Milk Samples

ABSTRACTRecent studies report the presence of fungal species in breast milk of healthy mothers, suggesting a potential role in infant mycobiome development. In the present work, we aimed to determine whether the healthy human breast milk mycobiota is influenced by geographical location and mode of delivery, as well as to investigate its interaction with bacterial profiles in the same samples. A total of 80 mature breast milk samples from 4 different countries were analyzed by Illumina sequencing of the internal transcribed spacer 1 (ITS1) region, joining the 18S and 5.8S regions of the fungal rRNA region. Basidiomycota and Ascomycota were found to be the dominant phyla, withMalasseziaandDavidiellabeing the most prevalent genera across countries. A core formed byMalassezia, Davidiella, Sistotrema, andPenicilliumwas shared in the milk samples from the different origins, although specific shifts in mycobiome composition were associated with geographic location and delivery mode. The presence of fungi in the breast milk samples was further confirmed by culture and isolate characterization, and fungal loads were estimated by quantitative PCR (qPCR) targeting the fungal ITS1 region. Cooccurrence network analysis of bacteria and fungi showed complex interactions that were influenced by geographical location, mode of delivery, maternal age, and pregestational body mass index. The presence of a breast milk mycobiome was confirmed in all samples analyzed, regardless of the geographic origin.IMPORTANCEDuring recent years, human breast milk has been documented as a potential source of bacteria for the newborn. Recently, we have reported the presence of fungi in breast milk from healthy mothers. It is well known that environmental and perinatal factors can affect milk bacteria; however, the impact on milk fungi is still unknown. The current report describes fungal communities (mycobiota) in breast milk samples across different geographic locations and the influence of the mode of delivery. We also provide novel insights on bacterium-fungus interactions, taking into account environmental and perinatal factors. We identified a core of four genera shared across locations, consisting ofMalassezia, Davidiella, Sistotrema, andPenicillium, which have been reported to be present in the infant gut. Our data confirm the presence of fungi in breast milk across continents and support the potential role of breast milk in the initial seeding of fungal species in the infant gut.

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