scholarly journals Effects of co-formulants on the absorption and secretion of active substances in plant protection products in vitro

2021 ◽  
Author(s):  
Mawien Karaca ◽  
Benjamin Christian Fischer ◽  
Christian Tobias Willenbockel ◽  
Tewes Tralau ◽  
Philip Marx-Stoelting ◽  
...  
Keyword(s):  
Fluorescence Anisotropy ◽  
Plant Protection ◽  
Plant Protection Products ◽  
Qualitative And Quantitative ◽  
Mixture Effects ◽  
Dependent Inhibition ◽  
Surface Active ◽  
Active Substances ◽  
Do So

AbstractCurrently, the authorisation process for plant protection products (PPPs) relies on the testing of acute and topological toxicity only. Contrastingly, the evaluation of active substances includes a more comprehensive set of toxicity studies. Nevertheless, mixture effects of active ingredients and co-formulants may result in increased toxicity. Therefore, we investigated effects of surface active co-formulants on the toxicity of two PPPs focussing on qualitative and quantitative toxicokinetic effects on absorption and secretion. The respective products are based on the active substances abamectin and fluroxypyr-meptyl and were tested for cytotoxicity in the presence or absence of the corresponding surfactants and co-formulants using Caco-2 cells. In addition, the effect of co-formulants on increased cellular permeation was quantified using LC–MS/MS, while potential kinetic mixture effects were addressed by fluorescence anisotropy measurements and ATPase assays. The results show that surface active co-formulants significantly increase the cytotoxicity of the investigated PPPs, leading to more than additive mixture effects. Moreover, analytical investigations show higher efflux ratios of both active substances and the metabolite fluroxypyr upon combination with certain concentrations of the surfactants. The results further point to a significant and concentration-dependent inhibition of Pgp transporters by most of the surfactants as well as to increased membrane fluidity. Altogether, these findings strongly support the hypothesis that surfactants contribute to increased cytotoxicity of PPPs and do so by increasing the bioavailability of the respective active substances.

Toxicokinetic mixture effects of co-formulants and active substances in plant protection products in vitro

2021 ◽  
Vol 350 ◽  
pp. S237
Author(s):  
M. Karaca ◽  
B. Fischer ◽  
C.T. Willenbockel ◽  
P. Marx-Stoelting ◽  
D. Bloch
Keyword(s):  
Plant Protection ◽  
Plant Protection Products ◽  
Mixture Effects ◽  
Active Substances

Optimisation of an in vitro larvae test to test the effects of plant protection products on honeybee brood (Apis mellifera L.)

2014 ◽  
Vol 9 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Dorothee J. Lüken ◽  
Martina Janke ◽  
Friedrich-Wilhelm Lienau ◽  
Katharina Gerdel ◽  
Werner von der Ohe ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Plant Protection ◽  
Plant Protection Products

scholarly journals Application of the Threshold of Toxicological Concern (TTC) in Food Safety: Challenges and Opportunities

2021 ◽  
Vol 3 ◽  
Author(s):  
Rositsa Serafimova ◽  
Tamara Coja ◽  
George E. N. Kass
Keyword(s):  
Food Safety ◽  
Plant Protection ◽  
Plant Protection Products ◽  
The European Union ◽  
Use Of Data ◽  
Challenges And Opportunities ◽  
Guidance Documents ◽  
Threshold Of Toxicological Concern ◽  
Active Substances

The safety assessment of chemicals added or found in food has traditionally made use of data from in vivo studies performed on experimental animals. The nature and amount of data required to carry out a risk assessment is generally stipulated either in the different food legislations or in sectoral guidance documents. However, there are still cases where no or only limited experimental data are available or not specified by law, for example for contaminants or for some minor metabolites from active substances in plant protection products. For such cases, the Threshold of Toxicological Concern (TTC) can be applied. This review explores the use of the TTC approach in food safety in the European Union, in relation to the different food sectors, legal requirements and future opportunities.

scholarly journals Interactions of Ionic Liquids and Spirocyclic Compounds with Liposome Model Membranes. A Steady-State Fluorescence Anisotropy Study

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Antti H. Rantamäki ◽  
Wen Chen ◽  
Paulus Hyväri ◽  
Jussi Helminen ◽  
Gabriel Partl ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Steady State ◽  
Fluorescence Anisotropy ◽  
Active Compounds ◽  
Steady State Fluorescence ◽  
Structure Relation ◽  
Surface Active ◽  
Spirocyclic Compounds ◽  
Surface Active Compounds

AbstractUnderstanding the toxicity of ionic liquids (ILs) is crucial in the search of greener chemicals. By comparing in vivo toxicity and in vitro interactions determined between compounds and biomimetic lipid membranes, more detailed toxicity vs. structure relation can be obtained. However, determining the interactions between non-surface-active compounds and liposomes has been a challenging task. Organisational changes induced by ILs and IL-like spirocyclic compounds within 1,6-diphenyl-1,3,5-hexatriene-doped biomimetic liposomes was studied by steady-state fluorescence anisotropy technique. The extent of organisational changes detected within the liposome bilayers were compared to the toxicity of the compounds determined using Vibrio Fischeri bacteria. Four liposome compositions made of pure 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocoline (POPC) and mixtures of POPC, 1-palmitoyl-2-oleyl-sn-glycero-3-phosphoserine (POPS), and cholesterol (Chol) were tested as biomimetic models. Changes observed within the POPC/POPS/Chol 55:20:25 bilayers correlated the best with the toxicity results: ten out of twelve compounds followed the trend of increasing bilayer disorder – increasing toxicity. The study suggests that the toxicity of non-surface-active compounds is dependent on their ability to diffuse into the bilayers. The extent of bilayer’s organisational changes correlates rather well with the toxicity of the compounds. Highly sensitive technique, such as fluorescence anisotropy measurements, is needed for detecting subtle changes within the bilayer structures.

scholarly journals Current possibilities and prospects of using fungicides in forestry

2015 ◽  
Vol 76 (2) ◽  
pp. 191-206 ◽  
Author(s):  
Adam Okorski ◽  
Agnieszka Pszczółkowska ◽  
Tomasz Oszako ◽  
Justyna A. Nowakowska ◽  
Małgorzata Oszako
Keyword(s):  
Mycorrhizal Fungi ◽  
Fungicide Resistance ◽  
Plant Protection ◽  
Plant Protection Products ◽  
Legal Regulations ◽  
Effective Protection ◽  
Limited Availability ◽  
Forest Pathogens ◽  
Forest Nurseries ◽  
Active Substances

AbstractThe possibility of using chemicals in European forestry is extremely limited due to the binding legal regulations and specific conditions concerning the market of plant protection products. This is reflected in the limited availability of active fungicides in forestry. Due to this limitation, practitioners using fungicides in forest nurseries and forest cultivation must have substantial knowledge of the biology of pathogens to ensure satisfactorily effective protection.The work presented here provides an overview of the currently recommended fungicides in Polish forestry as well as the mechanisms of interaction between the active substances and the pathogen, the plant and mycorrhizal fungi. The risk of fungicide resistance, which has been insufficiently explored in the context of forest pathogens, is also discussed in this paper.

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