A Lateral Flow Immunoassay for the Rapid Detection of Ochratoxin A in Wine and Grape Must

2012 ◽  
Vol 60 (46) ◽  
pp. 11491-11497 ◽  
Author(s):  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Gianfranco Giraudi ◽  
Flavia Biagioli ◽  
Cinzia Passini ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Grape Must

A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice

2021 ◽  
Vol 336 ◽  
pp. 127710
Author(s):  
Liangwen Hao ◽  
Jing Chen ◽  
Xirui Chen ◽  
Tongtong Ma ◽  
Xiaoxia Cai ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Grape Juice ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals Rapid and Simple Detection of Ochratoxin A using Fluorescence Resonance Energy Transfer on Lateral Flow Immunoassay (FRET-LFI)

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 292
Author(s):  
Hyun-Kyung Oh ◽  
Hyou-Arm Joung ◽  
Minhyuk Jung ◽  
Hohjai Lee ◽  
Min-Gon Kim
Keyword(s):  
Energy Transfer ◽  
Ochratoxin A ◽  
Fluorescence Resonance Energy Transfer ◽  
Matrix Effect ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
The Matrix ◽  
Fluorescence Resonance

The detection of mycotoxins is crucial because of their toxicity in plants, animals, and humans. It is very important to determine whether food products are contaminated with mycotoxins such as ochratoxin A (OTA), as mycotoxins can survive heat treatments and hydrolysis. In this study, we designed a fluorescence resonance energy transfer (FRET)-based system that exploits antibody-antigen binding to detect mycotoxins more rapidly and easily than other currently available methods. In addition, we were able to effectively counteract the matrix effect in the sample by using a nitrocellulose membrane that enabled fluorescence measurement in coffee samples. The developed FRET on lateral flow immunoassay (FRET-LFI) system was used to detect OTA at a limit of detection (LOD) of 0.64 ng∙mL−1, and the test can be completed in only 30 min. Moreover, OTA in coffee samples was successfully detected at a LOD of 0.88 ng∙mL−1, overcoming the matrix effect, owing to the chromatographic properties of the capillary force of the membrane. We believe that the developed system can be used as a powerful tool for the sensitive diagnosis of harmful substances such as mycotoxins and pesticides for environmental and food quality control monitoring.

scholarly journals Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 794
Author(s):  
Arpasiri Srisrattakarn ◽  
Patcharaporn Tippayawat ◽  
Aroonwadee Chanawong ◽  
Ratree Tavichakorntrakool ◽  
Jureerut Daduang ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Rapid Detection ◽  
Protein A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Staphylococcal Protein A ◽  
Highly Sensitive ◽  
Sophisticated Equipment ◽  
Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC

2017 ◽  
Vol 200 ◽  
pp. 101-106 ◽  
Author(s):  
Constanze Seidel ◽  
Sonja Peters ◽  
Erik Eschbach ◽  
Andrea T. Feßler ◽  
Boris Oberheitmann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Resistance Genes ◽  
Rapid Detection ◽  
Methicillin Resistance ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

A lateral flow immunoassay for measuring ochratoxin A: Development of a single system for maize, wheat and durum wheat

Food Control ◽  
2011 ◽  
Vol 22 (12) ◽  
pp. 1965-1970 ◽  
Author(s):  
Anfossi Laura ◽  
D’Arco Gilda ◽  
Baggiani Claudio ◽  
Giovannoli Cristina ◽  
Giraudi Gianfranco
Keyword(s):  
Durum Wheat ◽  
Ochratoxin A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Single System

scholarly journals Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1805
Author(s):  
Saoussen Oueslati ◽  
Camille Gonzalez ◽  
Hervé Volland ◽  
Vincent Cattoir ◽  
Sandrine Bernabeu ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Clinical Microbiology ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Bacterial Cells ◽  
Efficient Management ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Prolonged Hospital

Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria.

Development of a lateral flow immunoassay strip for rapid detection of rotavirus in fecal samples

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Do Thi Thu Ha ◽  
Ngo Thu Huong ◽  
Nguyen Dang Hien ◽  
Le Thi Luan ◽  
Luong Trinh Thuy Linh ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Fecal Samples
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