Polydopamine nanodots-based cost-effective nanoprobe for glucose detection and intracellular imaging

Carbon-based electrodes have demonstrated great promise as electrochemical transducers in the development of biosensors. More recently, laser-induced graphene (LIG), a graphene derivative, appears as a great candidate due to its superior electron transfer characteristics, high surface area and simplicity in its synthesis. The continuous interest in the development of cost-effective, more stable and reliable biosensors for glucose detection make them the most studied and explored within the academic and industry community. In this work, the electrochemistry of glucose oxidase (GOx) adsorbed on LIG electrodes is studied in detail. In addition to the well-known electroactivity of free flavin adenine dinucleotide (FAD), the cofactor of GOx, at the expected half-wave potential of −0.490 V vs. Ag/AgCl (1 M KCl), a new well-defined redox pair at 0.155 V is observed and shown to be related to LIG/GOx interaction. A systematic study was undertaken in order to understand the origin of this activity, including scan rate and pH dependence, along with glucose detection tests. Two protons and two electrons are involved in this reaction, which is shown to be sensitive to the concentration of glucose, restraining its origin to the electron transfer from FAD in the active site of GOx to the electrode via direct or mediated by quinone derivatives acting as mediators.

Download Full-text

Simple and Cost-Effective Glucose Detection Based on Carbon Nanodots Supported on Silver Nanoparticles

Analytical Chemistry ◽

10.1021/acs.analchem.6b04259 ◽

2016 ◽

Vol 89 (2) ◽

pp. 1323-1328 ◽

Cited By ~ 94

Author(s):

Jin-Liang Ma ◽

Bin-Cheng Yin ◽

Xin Wu ◽

Bang-Ce Ye

Keyword(s):

Silver Nanoparticles ◽

Cost Effective ◽

Carbon Nanodots ◽

Glucose Detection

Download Full-text

A Disposable Paper-Based Electrochemical Sensor Made of Pencil Graphite and Its Modification with Au for Detection of Glucose in Alkaline Solutions

10.26434/chemrxiv.12102975.v2 ◽

2020 ◽

Author(s):

Mustafa Sen

Keyword(s):

Environmental Monitoring ◽

Electrochemical Sensor ◽

Point Of Care ◽

Low Cost ◽

Cost Effective ◽

Alkaline Solutions ◽

Glucose Detection ◽

Electrochemical Modification ◽

Point Of Care Diagnostics

Paper-based sensors have great potential to be used in a variety of fields ranging from environmental monitoring to clinical and point-of-care diagnostics. These sensors are disposable, cost effective, flexible and easy to use. The aim of this study was to fabricate a low cost, disposable, reliable and easy to use paper-based electrochemical sensor and its electrochemical modification with nanostructured Au for glucose detection in alkaline solutions.

Download Full-text

Cost-effective CuO nanotube electrodes for energy storage and non-enzymatic glucose detection

RSC Advances ◽

10.1039/c4ra08230c ◽

2014 ◽

Vol 4 (87) ◽

pp. 46814-46822 ◽

Cited By ~ 27

Author(s):

Yang Lu ◽

Kangwen Qiu ◽

Deyang Zhang ◽

Jing Lin ◽

Jinyou Xu ◽

...

Keyword(s):

Energy Storage ◽

Cost Effective ◽

Glucose Detection ◽

Copper Foils

Porous CuO nanotube electrodes synthesized by the in situ oxidation of copper foils exhibit superior pseudocapacitive and electrocatalytic performances.

Download Full-text

A Disposable Paper-Based Electrochemical Sensor Made of Pencil Graphite and Its Modification with Au for Detection of Glucose in Alkaline Solutions

10.26434/chemrxiv.12102975.v1 ◽

2020 ◽

Author(s):

Mustafa Sen

Keyword(s):

Environmental Monitoring ◽

Electrochemical Sensor ◽

Point Of Care ◽

Low Cost ◽

Cost Effective ◽

Alkaline Solutions ◽

Glucose Detection ◽

Electrochemical Modification ◽

Point Of Care Diagnostics

Paper-based sensors have great potential to be used in a variety of fields ranging from environmental monitoring to clinical and point-of-care diagnostics. These sensors are disposable, cost effective, flexible and easy to use. The aim of this study was to fabricate a low cost, disposable, reliable and easy to use paper-based electrochemical sensor and its electrochemical modification with nanostructured Au for glucose detection in alkaline solutions.

Download Full-text

Solid-State rGO-PEDOT:PSS Transducing Material for Cost-Effective Enzymatic Sensing

Biosensors ◽

10.3390/bios9010036 ◽

2019 ◽

Vol 9 (1) ◽

pp. 36 ◽

Cited By ~ 7

Author(s):

Firdaus Abd-Wahab ◽

Habibah Abdul Guthoos ◽

Wan Wan Salim

Keyword(s):

Electron Microscopy ◽

Cyclic Voltammetry ◽

Graphene Oxide ◽

Construction Material ◽

Cost Effective ◽

Glucose Detection ◽

Electrochemical Characteristics ◽

Carbon Electrode ◽

Screen Printed Carbon Electrode ◽

Reduced Graphene

Performance of a sensing device is dependent on its construction material, especially for components that are directly involved in transporting and translating signals across the device. Understanding the morphology and characteristics of the material components is therefore crucial in the development of any sensing device. This work examines the morphological and electrochemical characteristics of reduced graphene oxide interspersed with poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (rGO-PEDOT:PSS) used as a transducer material deposited on a commercially available screen-printed carbon electrode (SPCE). Electron microscopy shows that PEDOT:PSS is interspersed between rGO layers. Raman and XRD analyses suggest that the graphene crystallinity in GO-PEDOT:PSS and rGO-PEDOT:PSS remains intact. Instead, PEDOT:PSS undergoes a change in structure to allow PEDOT to blend into the graphene structure and partake in the π-π interaction with the surface of the rGO layers. Incorporation of PEDOT:PSS also appears to improve the electrochemical behavior of the composite, leading to a higher peak current of 1.184 mA, as measured by cyclic voltammetry, compared to 0.522 mA when rGO is used alone. The rGO-PEDOT:PSS transducing material blended with glucose oxidase was tested for glucose detection. The sensitivity of glucose detection was shown to be 57.3 µA/(mM·cm2) with a detection limit of 86.8 µM.

Download Full-text

A Disposable Paper-Based Electrochemical Sensor Made of Pencil Graphite and Its Modification with Au for Detection of Glucose in Alkaline Solutions

10.26434/chemrxiv.12102975 ◽

2020 ◽

Author(s):

Mustafa Sen

Keyword(s):

Environmental Monitoring ◽

Electrochemical Sensor ◽

Point Of Care ◽

Low Cost ◽

Cost Effective ◽

Alkaline Solutions ◽

Glucose Detection ◽

Electrochemical Modification ◽

Point Of Care Diagnostics

Paper-based sensors have great potential to be used in a variety of fields ranging from environmental monitoring to clinical and point-of-care diagnostics. These sensors are disposable, cost effective, flexible and easy to use. The aim of this study was to fabricate a low cost, disposable, reliable and easy to use paper-based electrochemical sensor and its electrochemical modification with nanostructured Au for glucose detection in alkaline solutions.

Download Full-text

The application of electron microscopy to diagnosis in gynecologic neoplasms and tumor-like conditions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110787 ◽

1984 ◽

Vol 42 ◽

pp. 102-105

Author(s):

Lawrence M. Roth

Keyword(s):

Electron Microscopy ◽

Reproductive Tract ◽

Malignant Tumors ◽

Frozen Section ◽

Cost Effective ◽

Female Reproductive Tract ◽

Ultrastructural Examination ◽

Specific Diagnosis ◽

Potential Value ◽

Gynecologic Neoplasms

The female reproductive tract may be the site of a wide variety of benign and malignant tumors, as well as non-neoplastic tumor-like conditions, most of which can be diagnosed by light microscopic examination including special stains and more recently immunoperoxidase techniques. Nevertheless there are situations where ultrastructural examination can contribute substantially to an accurate and specific diagnosis. It is my opinion that electron microscopy can be of greatest benefit and is most cost effective when applied in conjunction with other methodologies. Thus, I have developed an approach which has proved useful for me and may have benefit for others. In cases where it is deemed of potential value, glutaraldehyde-fixed material is obtained at the time of frozen section or otherwise at operation. Coordination with the gynecologic oncologist is required in the latter situation. This material is processed and blocked and is available if a future need arises.

Download Full-text

The IBM-PC in electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100165525 ◽

1996 ◽

Vol 54 ◽

pp. 612-613

Author(s):

James F. Mancuso

Keyword(s):

Head Start ◽

Image Acquisition ◽

Cost Effective ◽

Financial Applications ◽

Number Of Factors ◽

Instrument Control ◽

The Cost ◽

Training And Support ◽

Control Image ◽

Ibm Pc

IBM PC compatible computers are widely used in microscopy for applications ranging from control to image acquisition and analysis. The choice of IBM-PC based systems over competing computer platforms can be based on technical merit alone or on a number of factors relating to economics, availability of peripherals, management dictum, or simple personal preference.IBM-PC got a strong “head start” by first dominating clerical, document processing and financial applications. The use of these computers spilled into the laboratory where the DOS based IBM-PC replaced mini-computers. Compared to minicomputer, the PC provided a more for cost-effective platform for applications in numerical analysis, engineering and design, instrument control, image acquisition and image processing. In addition, the sitewide use of a common PC platform could reduce the cost of training and support services relative to cases where many different computer platforms were used. This could be especially true for the microscopists who must use computers in both the laboratory and the office.

Download Full-text

A Review of 21 iPad Applications for Augmentative and Alternative Communication Purposes

Perspectives on Augmentative and Alternative Communication ◽

10.1044/aac21.2.60 ◽

2012 ◽

Vol 21 (2) ◽

pp. 60-71 ◽

Cited By ~ 24

Author(s):

Ashley Alliano ◽

Kimberly Herriger ◽

Anthony D. Koutsoftas ◽

Theresa E. Bartolotta

Keyword(s):

Augmentative And Alternative Communication ◽

Cost Effective ◽

Alternative Form ◽

Alternative Communication ◽

Text To Speech ◽

Reference Guide ◽

Expressive Communication ◽

Communication Needs ◽

User Friendly ◽

Ipad Applications

Abstract Using the iPad tablet for Augmentative and Alternative Communication (AAC) purposes can facilitate many communicative needs, is cost-effective, and is socially acceptable. Many individuals with communication difficulties can use iPad applications (apps) to augment communication, provide an alternative form of communication, or target receptive and expressive language goals. In this paper, we will review a collection of iPad apps that can be used to address a variety of receptive and expressive communication needs. Based on recommendations from Gosnell, Costello, and Shane (2011), we describe the features of 21 apps that can serve as a reference guide for speech-language pathologists. We systematically identified 21 apps that use symbols only, symbols and text-to-speech, and text-to-speech only. We provide descriptions of the purpose of each app, along with the following feature descriptions: speech settings, representation, display, feedback features, rate enhancement, access, motor competencies, and cost. In this review, we describe these apps and how individuals with complex communication needs can use them for a variety of communication purposes and to target a variety of treatment goals. We present information in a user-friendly table format that clinicians can use as a reference guide.

Download Full-text