The influence of the pre-treatment of apple cell wall samples on their functional properties

2001 ◽  
Vol 212 (6) ◽  
pp. 630-635 ◽  
Author(s):  
Silke Vetter ◽  
Herbert Kunzek ◽  
Bernhard Senge
Keyword(s):  
Cell Wall ◽  
Functional Properties ◽  
Pre Treatment

Near Infrared Spectroscopy Estimation of Feeding Value of Forage Perennial Grasses in Breeding Programmes by Global and Specific Calibrations. Estimation of Chemical Composition and Digestibility

1998 ◽  
Vol 6 (1) ◽  
pp. 153-165 ◽  
Author(s):  
Y. Naydenova ◽  
P. Tomov ◽  
P. Dardenne
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Predictive Models ◽  
Near Infrared ◽  
Perennial Grasses ◽  
Standard Errors ◽  
Breeding Programmes ◽  
Feeding Value ◽  
Pre Treatment ◽  
Better Than

Near infrared (NIR) spectral analysis with a NIRSystems 6500 monochromator was applied to evaluate accuracy of predictive models for forage quality in clone breeding processes of the original Bulgarian varieties over different cuts and years. The varieties were the perennial grasses: Dactylis glomerata L., Festuca arundinacea Schreb. and Bromus inermis Leyss. Global calibrations for the 418 perennial grass samples and specific calibrations for each single grass species and internal cross-validations were performed by the PLS regression method. The effect of different spectral data pre-treatments was investigated on the residual standard errors of the NIR predictive models. Among 60 calibration equations, the model with the lowest SECV value was retained for each parameter in each database. No particular data pre-treatment was really better than the other ones. Generally, the best results of the global calibrations were obtained with SNVD and MSC. For the specific calibrations, SNVD and WMSC were the best treatments. In both cases, the first or second derivatives were needed after the first pre-treatment. Chemical composition and in vitro enzymatic digestibility of clones were predicted with accuracy similar to that of classical laboratory methods. For the cell wall component contents, the standard errors of cross-validation SECV(%DM) ranged from 0.49 for ADL ( Festuca) to 2.02 for NDF ( Dactylis). The digestibilities of dry and organic matter, IVDMD and IVOMD, were estimated with SECVs from 2.6 to 3.0%, the relative intake, from 0.06 to 0.09 rel% body weight and the relative feeding value, from 4.39 to 5.64 rel%. The global calibration models offer an acceptable accuracy for the estimation of the cell wall nutrient contents, the digestibility and the nutritive value. The standard errors of prediction of specific single species calibrations with smaller numbers of terms were lower in 60% of the cases than those obtained from the best global calibrations with higher numbers of terms. On average, SECVs from specific calibrations are better than those from global calibrations, but the differences are quite small, and for the prediction of totally new samples (new crops, another year), the global calibrations will detect less outlier samples. Even with very high variability between cuts and years, NIR spectroscopy is able via ANOVA GL Models to sort clones on their feeding value and to provide relevant information for the breeding programmes.

scholarly journals Perennial halophyte Salicornia neei Lag.: Cell wall composition and functional properties of its biopolymers

2020 ◽  
pp. 128659
Author(s):  
Matias R. Villarreal ◽  
Diego A. Navarro ◽  
Nora M.A. Ponce ◽  
Ana M. Rojas ◽  
Carlos A. Stortz
Keyword(s):  
Cell Wall ◽  
Functional Properties ◽  
Cell Wall Composition

Changes in microstructure and functional properties of papaya as affected by osmotic pre-treatment combined with freeze-drying

2018 ◽  
Vol 12 (2) ◽  
pp. 1028-1037 ◽  
Author(s):  
Patchimaporn Udomkun ◽  
Dimitrios Argyropoulos ◽  
Marcus Nagle ◽  
Busarakorn Mahayothee ◽  
Alamu Emmanuel Oladeji ◽  
...  
Keyword(s):  
Functional Properties ◽  
Freeze Drying ◽  
Pre Treatment

Über die Rezeptorsubstanz für den Phagen T5

1954 ◽  
Vol 9 (9) ◽  
pp. 573-579 ◽  
Author(s):  
Wolfhard Weidel ◽  
Gebhard Koch ◽  
Karl Bobosch
Keyword(s):  
Cell Wall ◽  
B Cell ◽  
Functional Properties ◽  
Phage Infection ◽  
Receptor Activity ◽  
Coli Cell ◽  
Infection Mechanism ◽  
Entire Structure

Weak alkali extracts from the intact coli-B-cell a lipo-glycoproteid which after purification shows receptor activity against the phage T5 only. Purification of this material is described and data are given on physicochemical, chemical and functional properties of the T5 receptor substance as well as of receptor-inactive material extracted from B/1,5. From all the data available it is tried to form a picture of the entire structure of the coli cell wall and to connect it with the phage infection mechanism.

Thermal analysis of plant cell wall materials depending on the chemical structure and pre-treatment prior to drying

2001 ◽  
Vol 213 (4-5) ◽  
pp. 395-404 ◽  
Author(s):  
Ralf Godeck ◽  
Herbert Kunzek ◽  
Robert Kabbert
Keyword(s):  
Thermal Analysis ◽  
Cell Wall ◽  
Plant Cell ◽  
Chemical Structure ◽  
Plant Cell Wall ◽  
Wall Materials ◽  
Pre Treatment ◽  
Cell Wall Materials

Effect of power ultrasound pre-treatment on the physical and functional properties of reconstituted milk protein concentrate

2014 ◽  
Vol 124 ◽  
pp. 11-18 ◽  
Author(s):  
Sun Yanjun ◽  
Chen Jianhang ◽  
Zhang Shuwen ◽  
Li Hongjuan ◽  
Lu Jing ◽  
...  
Keyword(s):  
Functional Properties ◽  
Milk Protein ◽  
Protein Concentrate ◽  
Milk Protein Concentrate ◽  
Power Ultrasound ◽  
Pre Treatment ◽  
Reconstituted Milk

scholarly journals Monoclonal antibodies targeting surface exposed epitopes of Candida albicans cell wall proteins confer in vivo protection in an infection model

2021 ◽  
Author(s):  
Soumya Palliyil ◽  
Mark Mawer ◽  
Sami Alwafi ◽  
Lily Fogg ◽  
Giuseppe Buda De Cesare ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Fungal Infections ◽  
Yeast Cells ◽  
Infection Model ◽  
Cell Wall Proteins ◽  
Peptide Antigens ◽  
Log Reduction ◽  
Pre Treatment

MAb based immunotherapies targeting systemic and deep-seated fungal infections are still in their early stages of development with currently no licensed antifungal mAbs available. The cell wall glycoproteins of Candida albicans are potential targets for therapeutic antibody generation due to their extracellular location and key involvement in fungal pathogenesis. We describe phage display based generation of recombinant human antibodies specifically targeting two key cell wall proteins (CWPs) in C. albicans - Utr2 and Pga31, using peptide antigens representing the surface exposed regions of CWPs at elevated levels during in vivo infection. Reformatted mAbs preferentially recognised C. albicans hyphal forms compared to yeast cells and an increased binding in cells pre-treated with caspofungin. In macrophage interaction assays, mAb pre-treatment resulted in a faster engulfment of C. albicans cells suggesting opsonophagocytosis. Finally, in a series of clinically predictive, mouse models of systemic candidiasis, our lead mAb achieved an improved survival (83%) and several log reduction of fungal burden in the kidneys, similar to levels achieved for the fungicidal drug caspofungin, and superior to any anti-Candida mAb.

scholarly journals Arabidopsis GELP7 is plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency.

2021 ◽  
Author(s):  
Lavi Rastogi ◽  
Aniket Anant Chaudhari ◽  
Raunak Sharma ◽  
Prashant Pawar
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Secondary Cell Wall ◽  
Acetyl Xylan Esterase ◽  
Acetyl Content ◽  
Transgenic Lines ◽  
Saccharification Efficiency ◽  
Cellulose Digestibility ◽  
Pre Treatment ◽  
Post Synthesis

Abstract Acetyl substitution on the xylan chain is critical for stable interaction with cellulose and other cell wall polymers in the secondary cell wall. Xylan acetylation pattern is governed by Golgi and extracellular localized acetyl xylan esterase (AXE). We investigated the role of Arabidopsis clade Id from the GDSL esterase/lipase or GELP family in polysaccharide deacetylation. The investigation of the AtGELP7 T-DNA mutant line showed a decrease in stem esterase activity and an increase in stem acetyl content. We further generated overexpressor AtGELP7 transgenic lines, and these lines showed a decrease in xylan acetylation in comparison with wild type plants. Therefore, we have named this enzyme as AtAXE1. The subcellular localization studies showed that the AtAXE1 enzyme is secreted out, associated with the plasma membrane and involved in xylan de-esterification post-synthesis. The cellulose digestibility was improved in AtAXE1 overexpressor lines without pre-treatment, after alkali and xylanases pre-treatment. Furthermore, we have also established that the AtGELP7 gene is upregulated in the overexpressor line of AtMYB46, which is a secondary cell wall specific transcription factor. This transcriptional regulation can drive AtGELP7 or AtAXE1 to perform de-esterification of xylan in a tissue-specific manner.

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