Laccase production and pellet morphology of Coprinopsis cinerea transformants in liquid shake flask cultures

The solubility of the compound is a crucial task for new drug design. Quinone is a promising candidate to develop as a new drug. In this research, the synthesis of 1,4-benzoquinone derivatives, that is, 2-(5-bromoamyl)-3,5-dimethyl-1,4-benzoquinone (2a) and 2-(5-bromoamyl)-5-methyl-1,4-benzoquinone (2b) were carried out by decarboxylation and insertion reaction of alkyl bromides. The product 2a and 2b are purified using SiO2 gel column chromatography and analyzed by UV-Visible, FT-IR, and NMR. The yield of 2a is 13.75%, and 2b is 4.04%. The solubility of 2a and 2b, expressed by log P, is measured in the n-octanol/water (3:7 (v/v)) system by the shake flask method. The log P of 2a and 2b are 2.99 and 1.36, respectively. It is showed that the log P of 2a is higher compared to 2b. The presence of two methyl substituents on the quinone ring of 2a supports the increase of hydrophobicity of the compound in the n-octanol/water system.

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Recombinant Laccase Production Optimization in Pichia pastoris by Response Surface Methodology and Its Application in the Biodegradation of Octyl Phenol and 4-Tert-Octylphenol

Catalysis Letters ◽

10.1007/s10562-021-03682-w ◽

2021 ◽

Author(s):

Qi Li ◽

Changsheng Chai ◽

Yitong Du ◽

Junli Cai ◽

Linguo Zhao

Keyword(s):

Response Surface Methodology ◽

Pichia Pastoris ◽

Response Surface ◽

Production Optimization ◽

Laccase Production ◽

Recombinant Laccase ◽

Octyl Phenol

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Lanthanum: a novel inducer for enhancement of fungal laccase production by Shiraia bambusicola

Journal of Rare Earths ◽

10.1016/j.jre.2020.12.012 ◽

2020 ◽

Author(s):

Yue Wang ◽

Xiang Zhang ◽

Cansong Lu ◽

Xinping Li ◽

Jianqin Zhou ◽

...

Keyword(s):

Laccase Production ◽

Fungal Laccase ◽

Shiraia Bambusicola

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PRODUCTION OF CYTASES ACTIVE ON BARLEY GUM BY BACTERIA OF THE GENUS BACILLUS

Canadian Journal of Botany ◽

10.1139/b53-004 ◽

1953 ◽

Vol 31 (1) ◽

pp. 28-32 ◽

Cited By ~ 1

Author(s):

A. C. Blackwood

Keyword(s):

Bacillus Subtilis ◽

Enzymatic Activity ◽

Nitrogen Source ◽

Freeze Drying ◽

Shake Flask ◽

Sole Nitrogen Source ◽

Genus Bacillus ◽

Original Activity ◽

Bacterial Cultures ◽

Bacillus Genus

One hundred and fourteen bacterial cultures representing most of the species in the Bacillus genus were tested for the production of extracellular barley gum cytase. Assays were made on shake-flask cultures grown on a medium containing glucose and yeast extract. Although all the organisms had some enzymatic activity, certain strains of Bacillus subtilis gave the best yields of cytase. On a medium with asparagine as the sole nitrogen source even higher yields were obtained. The crude cytase preparations were stable and after freeze-drying most of the original activity remained.

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A Novel Method for Preparation of Antimicrobial Cellulose Textile by Surface Carbamatization and N-Chlorination

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.399-401.1088 ◽

2011 ◽

Vol 399-401 ◽

pp. 1088-1092

Author(s):

Liu Yang Wang ◽

Fang Ying Wang ◽

Biao Bing Wang

Keyword(s):

Nitrogen Content ◽

Shake Flask ◽

Antimicrobial Activities ◽

Sodium Hypochlorite Solution ◽

E Coli ◽

Cellulose Fabric ◽

Carbamate Group ◽

Novel Method ◽

Cellulose Fabrics ◽

Temperature Time

Surface carbamatization of cellulose fabric was accomplished through co-heating with urea. The carbamate group on textile surface was transformed to N-chlorocarbamate after exposure to sodium hypochlorite solution. Effect of carbamatization temperature, time on Nitrogen content of fabrics was investigated, and the effect of bleach technology on chlorine content on fabrics was also studied. Using shake flask method antimicrobial activities against E. coli and S. aureus were studied. The results showed that when carbamatization temperature was 138°C and the time was 3 hours, nitrogen content of cellulose fabric was the highest: 1.47%. Antibacterial ability of cellulose fabrics with 0.81mg/g chlorine against E. coli and S. aureus was 99.9999% and 100%, respectively.

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