Isolation and characterization of psychrotrophic bacteria from oil-reservoir water and oil sands

2001 ◽  
Vol 55 (6) ◽  
pp. 794-800 ◽  
Author(s):  
T. Kato ◽  
M. Haruki ◽  
T. Imanaka ◽  
M. Morikawa ◽  
S. Kanaya
Keyword(s):  
Oil Sands ◽  
Oil Reservoir ◽  
Reservoir Water ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleem Farooq ◽  
Ruqeya Nazir ◽  
Shabir Ahmad Ganai ◽  
Bashir Ahmad Ganai
Keyword(s):  
Specific Activity ◽  
Temperature Range ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Active Protease ◽  
Quantitative Screening ◽  
First Time ◽  
Low K

AbstractAs an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and Vmax 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.

Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir

Extremophiles ◽  
2001 ◽  
Vol 5 (2) ◽  
pp. 85-91 ◽  
Author(s):  
Margarita Miroshnichenko ◽  
Hans Hippe ◽  
Erko Stackebrandt ◽  
Nadezhda Kostrikina ◽  
Nikolai Chernyh ◽  
...  
Keyword(s):  
High Temperature ◽  
Western Siberia ◽  
Oil Reservoir ◽  
Isolation And Characterization

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

2012 ◽  
Author(s):  
Rosli Md. Illias ◽  
Seok Wei Ooi ◽  
Ahmad Kamal Idris ◽  
Wan Aizan Wan Abdul Rahman
Keyword(s):  
Oil Recovery ◽  
Sole Carbon Source ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Oil Reservoir ◽  
Genus Bacillus ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Naci Concentration

Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

Isolation and characterization of various types of organic matter in oil sands tailings sludge

Fuel ◽  
1990 ◽  
Vol 69 (12) ◽  
pp. 1522-1526 ◽  
Author(s):  
Luba S. Kotlyar ◽  
John A. Ripmeester ◽  
Bryan D. Sparks
Keyword(s):  
Organic Matter ◽  
Oil Sands ◽  
Isolation And Characterization ◽  
Oil Sands Tailings

scholarly journals Isolation and characterization of humic acids from Alberta oil sands and related materials

Fuel ◽  
1990 ◽  
Vol 69 (12) ◽  
pp. 1527-1536 ◽  
Author(s):  
Abdul Majid ◽  
John A. Ripmeester
Keyword(s):  
Humic Acids ◽  
Oil Sands ◽  
Isolation And Characterization ◽  
Alberta Oil Sands

Isolation and Characterization of Metal and Antibiotic Resistant Psychrotrophic Bacteria from Refrigerated Spoiled Food

2014 ◽  
Vol 69 (1) ◽  
Author(s):  
Somayeh Asadi ◽  
Shafinaz Shahir ◽  
Zaharah Ibrahim ◽  
Adibah Yahya ◽  
Madihah Md Salleh ◽  
...  
Keyword(s):  
Metal Ions ◽  
Inhibitory Concentration ◽  
Metal Resistance ◽  
Antibiotic Resistant ◽  
Psychrotrophic Bacteria ◽  
Cold Environments ◽  
Isolation And Characterization ◽  
Identity Match ◽  
Microbial Strain

The aim of this study was to isolate and characterize psychrotrophic bacteria resistant to antibiotic and metals from cold samples of refrigerated spoiled food and ice. Two isolates named H and F were successfully isolated from samples incubated at 10°C and 4°C, respectively. Both isolates were able to grow at 4°C, 10°C, 20°C and 30°C and exhibited multidrug resistance to antibiotics (chloramphenicol, ampicillin, tetracycline and kanamycin). Bacteria H and F were then further tested for their resistance to metal ions such as Cd2+, Zn2+, Cr 6+ and As 3+   at concentrations ranging from 0-10 mM. The results of the test revealed that the minimum inhibitory concentration (MIC) for isolate H was determined at 2 mM for all the metal ions. By comparison, isolate F was highly resistant to Cr6+, Cd2+ andZn2+ with MIC of 10 mM for these cations, while MIC for As3+ was 4 mM. The results of comparing 16S rRNA gens demonstrated that isolate F has 98% identity match with Bacillus sp. This strain could be used as a model microbial strain to understand the mechanisms of metal resistance and as a source of psychrotrophic enzymes with potential application for bioremediation of heavy metals in cold environments.

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