Construction, expression and characterisation of a single-chain diabody derived from a humanised anti-Lewis Y cancer targeting antibody using a heat-inducible bacterial secretion vector

2001 ◽  
Vol 50 (5) ◽  
pp. 241-250 ◽  
Author(s):  
Barbara E. Power ◽  
Joanne M. Caine ◽  
John E. Burns ◽  
Deborah R. Shapira ◽  
Meghan K. Hattarki ◽  
...  
Keyword(s):  
Cancer Targeting ◽  
Single Chain ◽  
Secretion Vector ◽  
Lewis Y ◽  
Bacterial Secretion

scholarly journals Development of a bispecific immune engager using a recombinant malaria protein

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Mie A. Nordmaj ◽  
Morgan E. Roberts ◽  
Emilie S. Sachse ◽  
Robert Dagil ◽  
Anne Poder Andersen ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Immune Evasion ◽  
Blood Cells ◽  
Xenograft Model ◽  
Cancer Targeting ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Conjugation System ◽  
Immune Mediated

AbstractAs an immune evasion and survival strategy, the Plasmodium falciparum malaria parasite has evolved a protein named VAR2CSA. This protein mediates sequestration of infected red blood cells in the placenta through the interaction with a unique carbohydrate abundantly and exclusively present in the placenta. Cancer cells were found to share the same expression of this distinct carbohydrate, termed oncofetal chondroitin sulfate on their surface. In this study we have used a protein conjugation system to produce a bispecific immune engager, V-aCD3, based on recombinant VAR2CSA as the cancer targeting moiety and an anti-CD3 single-chain variable fragment linked to a single-chain Fc as the immune engager. Conjugation of these two proteins resulted in a single functional moiety that induced immune mediated killing of a broad range of cancer cells in vitro and facilitated tumor arrest in an orthotopic bladder cancer xenograft model.

scholarly journals Functional Production of a Soluble and Secreted Single-Chain Antibody by a Bacterial Secretion System

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97367 ◽  
Author(s):  
Chiu-Min Cheng ◽  
Shey-Cherng Tzou ◽  
Ya-Han Zhuang ◽  
Chien-Chiao Huang ◽  
Chien-Han Kao ◽  
...  
Keyword(s):  
Secretion System ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Bacterial Secretion ◽  
Bacterial Secretion System

scholarly journals Tumor Targeting by a Multivalent Single-Chain Fv (scFv) Anti-Lewis Y Antibody Construct

2008 ◽  
Vol 23 (4) ◽  
pp. 411-424 ◽  
Author(s):  
Marcus P. Kelly ◽  
F.-T. Lee ◽  
Kiki Tahtis ◽  
Barbara E. Power ◽  
Fiona E. Smyth ◽  
...  
Keyword(s):  
Tumor Targeting ◽  
Single Chain ◽  
Single Chain Fv ◽  
Lewis Y

scholarly journals Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity

1997 ◽  
Vol 10 (4) ◽  
pp. 445-453 ◽  
Author(s):  
S. M. Kipriyanov ◽  
G. Moldenhauer ◽  
A. C. Martin ◽  
O. A. Kupriyanova ◽  
M. Little
Keyword(s):  
Amino Acid ◽  
Antibody Fragment ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
Amino Acid Mutations ◽  
Bacterial Secretion ◽  
Single Chain Fv Antibody

Expression of a synthetic gene encoding potato carboxypeptidase inhibitor using a bacterial secretion vector

Gene ◽  
1992 ◽  
Vol 116 (2) ◽  
pp. 129-138 ◽  
Author(s):  
Miguel A. Molina ◽  
Francesc X. Avilés ◽  
Enrique Querol
Keyword(s):  
Synthetic Gene ◽  
Gene Encoding ◽  
Secretion Vector ◽  
Carboxypeptidase Inhibitor ◽  
Bacterial Secretion

High-level bacterial secretion of single-chain αβ T-cell receptors

2005 ◽  
Vol 306 (1-2) ◽  
pp. 51-67 ◽  
Author(s):  
Jennifer Maynard ◽  
Erin J. Adams ◽  
Michelle Krogsgaard ◽  
Karin Petersson ◽  
Corey W. Liu ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Single Chain ◽  
Cell Receptors ◽  
High Level ◽  
Bacterial Secretion

scholarly journals Selection and Characterization of Single Chain Antibody Fragments Specific for Hsp90 as a Potential Cancer Targeting Molecule

2015 ◽  
Vol 16 (8) ◽  
pp. 19920-19935 ◽  
Author(s):  
Edyta Petters ◽  
Aleksandra Sokolowska-Wedzina ◽  
Jacek Otlewski
Keyword(s):  
Antibody Fragments ◽  
Cancer Targeting ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Potential Cancer

Expression of the pheromone 3-encoding gene of Euplotes octocarinatus using a novel bacterial secretion vector

Gene ◽  
1994 ◽  
Vol 150 (1) ◽  
pp. 187-192 ◽  
Author(s):  
Claudia Brünen-Nieweler ◽  
Frank Meyer ◽  
Klaus Heckmann
Keyword(s):  
Secretion Vector ◽  
Encoding Gene ◽  
Euplotes Octocarinatus ◽  
Bacterial Secretion

Cryo-electron microscopy of two-dimensional crystals of reduced type B botulinum neurotoxin

1992 ◽  
Vol 50 (1) ◽  
pp. 530-531
Author(s):  
P. F. Flicker ◽  
V.S. Kulkarni ◽  
J. P. Robinson ◽  
G. Stubbs ◽  
B. R. DasGupta
Keyword(s):  
Disulfide Bonds ◽  
Clostridium Botulinum ◽  
Structural Changes ◽  
Two Dimensional ◽  
Type B ◽  
Single Chain ◽  
Muscle Paralysis ◽  
Cryo Electron Microscopy ◽  
Disulfide Linkages ◽  
Intracellular Action

Botulinum toxin is a potent neurotoxin produced by Clostridium botulinum. The toxin inhibits release of neurotransmitter, causing muscle paralysis. There are several serotypes, A to G, all of molecular weight about 150,000. The protein exists as a single chain or or as two chains, with two disulfide linkages. In a recent investigation on intracellular action of neurotoxins it was reported that type B neurotoxin can inhibit the release of Ca++-activated [3H] norepinephrine only if the disulfide bonds are reduced. In order to investigate possible structural changes in the toxin upon reduction of the disulfide bonds, we have prepared two-dimensional crystals of reduced type B neurotoxin. These two-dimensional crystals will be compared with those of the native (unreduced) type B toxin.

Fine structure and properties of defects in naturally occurring silicates and oxides

1990 ◽  
Vol 48 (4) ◽  
pp. 460-461
Author(s):  
David R. Veblen
Keyword(s):  
Chemical Reactions ◽  
High Resolution Electron Microscopy ◽  
Extended Defects ◽  
Single Chain ◽  
Naturally Occurring ◽  
Resolution Electron ◽  
Fine Structures ◽  
Image Simulations ◽  
Similar Crystal Structure

Extended defects and interfaces control many processes in rock-forming minerals, from chemical reactions to rock deformation. In many cases, it is not the average structure of a defect or interface that is most important, but rather the structure of defect terminations or offsets in an interface. One of the major thrusts of high-resolution electron microscopy in the earth sciences has been to identify the role of defect fine structures in reactions and to determine the structures of such features. This paper will review studies using HREM and image simulations to determine the structures of defects in silicate and oxide minerals and present several examples of the role of defects in mineral chemical reactions. In some cases, the geological occurrence can be used to constrain the diffusional properties of defects.The simplest reactions in minerals involve exsolution (precipitation) of one mineral from another with a similar crystal structure, and pyroxenes (single-chain silicates) provide a good example. Although conventional TEM studies have led to a basic understanding of this sort of phase separation in pyroxenes via spinodal decomposition or nucleation and growth, HREM has provided a much more detailed appreciation of the processes involved.

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