Identification and Characterization of a Shuttle Plasmid with Antibiotic Resistance Gene from Staphylococcus aureus

2003 ◽  
Vol 46 (6) ◽  
pp. 413-417 ◽  
Author(s):  
Mausumi Bhakta ◽  
Manjusri Bal
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Shuttle Plasmid ◽  
Identification And Characterization

scholarly journals Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance

2007 ◽  
Vol 73 (21) ◽  
pp. 6885-6890 ◽  
Author(s):  
Thi Thu Hao Van ◽  
George Moutafis ◽  
Taghrid Istivan ◽  
Linh Thuoc Tran ◽  
Peter J. Coloe
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Antimicrobial Agents ◽  
Genomic Island ◽  
Antibiotic Resistance Gene ◽  
Food Samples ◽  
Salmonella Spp

ABSTRACT A study was conducted to examine the levels of Salmonella spp. contamination in raw food samples, including chicken, beef, pork, and shellfish, from Vietnam and to determine their antibiotic resistance characteristics. A total of 180 samples were collected and examined for the presence of Salmonella spp., yielding 91 Salmonella isolates. Sixty-one percent of meat and 18% of shellfish samples were contaminated with Salmonella spp. Susceptibility of all isolates to a variety of antimicrobial agents was tested, and resistance to tetracycline, ampicillin/amoxicillin, nalidixic acid, sulfafurazole, and streptomycin was found in 40.7%, 22.0%, 18.7%, 16.5%, and 14.3% of the isolates, respectively. Resistance to enrofloxacin, trimethoprim, chloramphenicol, kanamycin, and gentamicin was also detected (8.8 to 2.2%). About half (50.5%) of the isolates were resistant to at least one antibiotic, and multiresistant Salmonella isolates, resistant to at least three different classes of antibiotics, were isolated from all food types. One isolate from chicken (serovar Albany) contained a variant of the Salmonella genomic island 1 antibiotic resistance gene cluster. The results show that antibiotic resistance in Salmonella spp. in raw food samples from Vietnam is significant.

scholarly journals Multiplex characterization of human pathogens including species and antibiotic-resistance gene identification

2016 ◽  
Vol 65 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Ivan Barišić ◽  
Josefine Petzka ◽  
Silvia Schoenthaler ◽  
Klemens Vierlinger ◽  
Christa Noehammer ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Gene Identification ◽  
Human Pathogens

An Introduction to Molecular Hospital Epidemiology

1993 ◽  
Vol 14 (7) ◽  
pp. 395-396 ◽  
Author(s):  
Neil L. Barg
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Phage Typing ◽  
Epidemic Strain ◽  
Nosocomial Pathogen ◽  
Hospital Epidemiology ◽  
Unique Strain ◽  
Identification And Characterization ◽  
Capsular Typing

One of the primary goals of the hospital epidemiologist is the identification and characterization of nosocomial outbreaks. Outbreaks usually are identified by the recovery of a unique strain from a cluster of patients infected with a nosocomially acquired pathogen. Until recently, the microbiologic tools available to any hospital epidemiologist permitted identification of novel strains by speciation and antibiogram. Thus, most outbreak descriptions consisted of the identification of an unusual species or the appearance of a new antibiotic resistance phenotype in a recognized nosocomial pathogen. The emergence of enterococci in this decade or the emergence of methicillin-resistantStaphylococcus aureus(MRSA) in the 1970s am notable examples. Where resources have existed, additional methods have been used for further discrimination. Outbreak and endemic strains have been compared by phage typing, serologic typing, and capsular typing. For example, beta-hemolytic streptococci may be grouped by Lancefield antisera, andStaphylococcus aureusmay be grouped by phage typing. However, certain Lancefield groups and certain phage groups are common among epidemic and endemic isolates, which may prevent identification of a specific epidemic strain.

Mining the bacterial unknown proteome: identification and characterization of a novel family of highly conserved protective antigens in Staphylococcus aureus

2013 ◽  
Vol 455 (3) ◽  
pp. 273-284 ◽  
Author(s):  
Christina Schluepen ◽  
Enrico Malito ◽  
Ambra Marongiu ◽  
Markus Schirle ◽  
Elisabeth McWhinnie ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Vaccine Candidates ◽  
Protective Antigens ◽  
Specific Antigens ◽  
Prophylactic Vaccines ◽  
Identification And Characterization

There is growing concern regarding the acquisition of antibiotic resistance by Staphylococcus aureus and growing interest in developing prophylactic vaccines against this pathogen. In the present paper, we highlight the importance of the Csa family of staphylococcal specific antigens as novel vaccine candidates.

scholarly journals Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1004
Author(s):  
Virginie Libante ◽  
Nazim Sarica ◽  
Abbas Mohamad Ali ◽  
Chloé Gapp ◽  
Anissa Oussalah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Transfer Rate ◽  
Antibiotic Resistance Gene ◽  
Conjugative Transfer ◽  
Streptococcus Species ◽  
Integrative Conjugative Element ◽  
Transfer Apparatus ◽  
Origin Of Transfer

Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are integrated into an unrelated IME or in chromosomal sites. After labeling by an antibiotic resistance gene, the conjugative transfer of one of these IMEs (named IME_oriTs) and its host ICE was measured. Although the IME is integrated in an ICE, it does not transfer as a part of the host ICE (no cis-mobilization). The IME excises and transfers separately from the ICE (without impacting its transfer rate) using its own relaxase, distantly related to all known MobT relaxases, and integrates in the oriT of the ICE after transfer. Overall, IME_oriTs use MobT-encoding ICEs both as hosts and as helpers for conjugative transfer. As half of them carry lsa(C), they actively participate in the dissemination of lincosamide–streptogramin A–pleuromutilin resistance among Firmicutes.

Molecular Characterization of an Antibiotic Resistance Gene Cluster of Salmonella typhimuriumDT104

1999 ◽  
Vol 43 (4) ◽  
pp. 846-849 ◽  
Author(s):  
Connie E. Briggs ◽  
Pina M. Fratamico
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Antibiotic Resistance Gene ◽  
Phage Type ◽  
Antibiotic Resistance Genes ◽  
Emerging Pathogen ◽  
Resistant Phenotype

ABSTRACT Salmonella typhimurium phage type DT104 has become an important emerging pathogen. Isolates of this phage type often possess resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT resistance). The mechanism by which DT104 has accumulated resistance genes is of interest, since these genes interfere with treatment of DT104 infections and might be horizontally transferred to other bacteria, even to unrelated organisms. Previously, several laboratories have shown that the antibiotic resistance genes of DT104 are chromosomally encoded and involve integrons. The antibiotic resistance genes conferring the ACSSuT-resistant phenotype have been cloned and sequenced. These genes are grouped within two district integrons and intervening plasmid-derived sequences. This sequence is potentially useful for detection of multiresistant DT104.

scholarly journals Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132892 ◽  
Author(s):  
Lenka Gerzova ◽  
Vladimir Babak ◽  
Karel Sedlar ◽  
Marcela Faldynova ◽  
Petra Videnska ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Microbiota Composition ◽  
Gene Abundance ◽  
Eu Countries
Sign in / Sign up

Export Citation Format

Share Document