The Effect of Rhamnolipid Biosurfactant Produced by Pseudomonas fluorescens on Model Bacterial Strains and Isolates from Industrial Wastewater

2010 ◽  
Vol 62 (2) ◽  
pp. 427-433 ◽  
Author(s):  
Evgenia Vasileva-Tonkova ◽  
Anna Sotirova ◽  
Danka Galabova
Keyword(s):  
Pseudomonas Fluorescens ◽  
Industrial Wastewater ◽  
Bacterial Strains ◽  
Rhamnolipid Biosurfactant

Production and Properties of Biosurfactants from a Newly Isolated Pseudomonas fluorescens HW-6 Growing on Hexadecane

2006 ◽  
Vol 61 (7-8) ◽  
pp. 553-559 ◽  
Author(s):  
Evgenia Vasileva-Tonkova ◽  
Danka Galabova ◽  
Emilia Stoimenova ◽  
Zdravko Lalchev
Keyword(s):  
Pseudomonas Fluorescens ◽  
Industrial Wastewater ◽  
High Surface ◽  
Emulsifying Properties ◽  
Effective Surface ◽  
Mineral Oils ◽  
Air Water Interface ◽  
Polluted Sites ◽  
High Concentrations ◽  
High Surface Activity

The newly isolated from industrial wastewater Pseudomonas fluorescens strain HW-6 produced glycolipid biosurfactants at high concentrations (1.4-2.0 g l-1) when grown on hexadecane as a sole carbon source. Biosurfactants decreased the surface tension of the air/ water interface by 35 mN m-1 and possessed a low critical micelle concentration value of 20 mg l-1, which indicated high surface activity. They efficiently emulsified aromatic hydrocarbons, kerosene, n-paraffins and mineral oils. Biosurfactant production contributed to a significant increase in cell hydrophobicity correlated with an increased growth of the strain on hexadecane. The results suggested that the newly isolated strain of Ps. fluorescens and produced glycolipid biosurfactants with effective surface and emulsifying properties are very promising and could find application for bioremediation of hydrocarbon-polluted sites.

Effectiveness of 3 antagonistic bacterial isolates to control Rhizoctonia solani Kühn on lettuce and potato

2005 ◽  
Vol 51 (4) ◽  
pp. 345-353 ◽  
Author(s):  
Rita Grosch ◽  
Franziska Faltin ◽  
Jana Lottmann ◽  
A Kofoet ◽  
Gabriele Berg
Keyword(s):  
Rhizoctonia Solani ◽  
Pseudomonas Fluorescens ◽  
Biological Control Agent ◽  
Dry Mass ◽  
Control Agent ◽  
Disease Suppression ◽  
Growth Chamber ◽  
Bacterial Strains ◽  
Serratia Plymuthica ◽  
Suppression Effect

Rhizoctonia solani causes yield losses in numerous economically important European crops. To develop a biocontrol strategy, 3 potato-associated ecto- and endophytically living bacterial strains Pseudomonas fluorescens B1, Pseudomonas fluorescens B2, and Serratia plymuthica B4 were evaluated against R. solani in potato and in lettuce. The disease-suppression effect of the 3 biocontrol agents (BCAs) was tested in a growth chamber and in the field. In growth chamber experiments, all 3 BCAs completely or significantly limited the dry mass (DM) losses on lettuce and the disease severity (DS) caused by R. solani on potato sprouts. Strain B1 showed the highest suppression effect (52% on average) on potato. Under field conditions, the DS on both crops, which were bacterized, decreased significantly, and the biomass losses on lettuce decreased significantly as well. The greatest disease-suppression effect on potato was achieved by strain B1 (37%), followed by B2 (33%) and then B4 (31%), whereas the marketable tuber yield increased up to 12% (B1), 6% (B2), and 17% (B4) compared with the pathogen control at higher disease pressure. Furthermore, in all experiments, B1 proved to be the most effective BCA against R. solani. Therefore, this BCA could be a candidate for developing a commercial product against Rhizoctonia diseases. To our knowledge, this is the first report on the high potential of endophytes to be used as a biological control agent against R. solani under field conditions.Key words: biocontrol, Rhizoctonia solani, field grown lettuce and potato, antagonistic bacteria, endophytes.

Plant-endophyte synergism in constructed wetlands enhances the remediation of tannery effluent

2018 ◽  
Vol 77 (5) ◽  
pp. 1262-1270 ◽  
Author(s):  
Sobia Ashraf ◽  
Muhammad Afzal ◽  
Khadeeja Rehman ◽  
Muhammad Naveed ◽  
Zahir Ahmad Zahir
Keyword(s):  
Industrial Wastewater ◽  
Bacterial Population ◽  
Ecological Engineering ◽  
Tannery Effluent ◽  
Inorganic Contaminants ◽  
Bacterial Strains ◽  
Severe Damage ◽  
Similar Reduction ◽  
Combined Action ◽  
Brachiaria Mutica

Abstract Liquid effluent produced from tanning industries is loaded with organic and inorganic contaminants, particularly heavy metals, which may cause severe damage to the ecosystem. Constructed wetland (CW) is a promising product of the research in the field of ecological engineering which helps to overcome aquatic pollution. This investigation aims to develop a plant–endophyte synergism in CW for the efficient remediation of tannery effluent. In a vertical flow CW, Brachiaria mutica was vegetated and augmented with three endophytic bacterial strains. Results showed a reduction of 82% in COD, 94% in BOD5, and 95% in Cr by plant–endophyte synergism in CWs and it was significantly higher than the use of plants alone. Similarly, nutrients (N and P), lipids, ion content, SO42−, and Cl− showed similar reduction by the combined action of endophytes and B. mutica in CWs. The endophytes inoculation enhanced bacterial population in different compartments of the plants vegetated in CWs and the maximum was observed in the roots. This study revealed that plant–endophyte synergism in CWs can enhance the remediation of industrial wastewater.

Bacterial diversity in antibiotic wastewater treatment

2013 ◽  
Vol 68 (12) ◽  
pp. 2676-2682 ◽  
Author(s):  
J. Han ◽  
L. Y. Wang ◽  
B. Y. Cai
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Diversity ◽  
Industrial Wastewater ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Diversity Index ◽  
Batch Reactor ◽  
Community Analysis ◽  
Bacterial Strains ◽  
Wiener Diversity Index ◽  
Gradient Gel Electrophoresis

The bacterial diversity of an antibiotic industrial wastewater treatment system was analyzed to provide the information required for further optimization of this process and for identification of bacterial strains that perform improved degradation of antibiotic industrial wastewater. The total bacterial DNA of samples collected at three stages (aeration, precipitation, and idle) during the sequencing batch reactor (SBR) process were analyzed by polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) of the 16 s rDNA V3 regions. Community analysis was conducted in terms of the richness value (S), the dominance degree and the Shannon–Wiener diversity index (H). Rich bacterial diversity was apparent in the aeration stage of the SBR process, and the number of bands in the aeration stage was more abundant than that in the precipitation and idle stages. The DGGE analysis showed 15 bands, six of which were uncultured bacteria, and included one anaerobic and five aerobic bacteria. The microbial community in the aeration stage was the most complex of the whole SBR process, while the dominant bacteria differed in each reaction stage. These results demonstrate the cyclical dynamic changes in the bacterial population during the SBR process for the treatment of antibiotic industrial wastewater.

Influence of NaCl and NaNO3 on Sinigrin Hydrolysis by Foodborne Bacteria

2011 ◽  
Vol 74 (12) ◽  
pp. 2162-2168 ◽  
Author(s):  
S. HERZALLAH ◽  
M. LARA LLEDÓ ◽  
R. HOLLEY
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Fluorescens ◽  
Starter Culture ◽  
Allyl Isothiocyanate ◽  
Escherichia Coli O157 ◽  
Bacterial Strains ◽  
Natural Antimicrobial ◽  
Staphylococcus Carnosus ◽  
Factors Influencing ◽  
Endogenous Plant

The glucosinolate sinigrin (SNG) is converted by endogenous plant myrosinase or by bacterial myrosinase-like activity to form the potent antimicrobial allyl isothiocyanate. In order to use SNG as a natural antimicrobial precursor in food, it became important to better understand the ability of bacteria to synthesize the enzyme(s) and understand factors influencing this synthesis at a constant SNG concentration. Eight spoilage, pathogenic, or starter culture bacteria were grown separately in medium containing individual or combined salts with SNG. SNG degradation by the bacteria and the formation of its major degradation product, allyl isothiocyanate, were followed for 12 days at 30 or 35°C. The bacterial strains varied in their ability to metabolize SNG, and this was enhanced by NaCl and/or NaNO3. SNG hydrolysis took place after 4 days, and the greatest amount occurred by day 12. At 12 days, Escherichia coli O157:H7 showed the greatest capacity to hydrolyze SNG (45.3% degradation), followed by Staphylococcus carnosus (44.57%), while Pseudomonas fluorescens was not active against SNG. The ability of tested strains to metabolize SNG, in decreasing order, was as follows: Escherichia coli O157:H7 > Staphylococcus carnosus > Staphylococcus aureus > Pediococcus pentosaceus > Salmonella Typhimurium > Listeria monocytogenes > Enterococcus faecalis > Pseudomonas fluorescens.

scholarly journals Two-step treatment of harmful industrial wastewater: an analysis of microbial reactor with integrated membrane retention for benzene and toluene removal

2015 ◽  
Vol 17 (4) ◽  
pp. 15-22 ◽  
Author(s):  
Anna Trusek-Holownia ◽  
Andrzej Noworyta
Keyword(s):  
Pseudomonas Fluorescens ◽  
Industrial Wastewater ◽  
Membrane Bioreactor ◽  
Membrane Separation ◽  
Integrated System ◽  
Integrated Process ◽  
Wastewater Purification ◽  
Toluene Removal ◽  
Bioreactor Operation ◽  
Operation Parameters

Abstract Standards for highly toxic and carcinogenic pollutants impose strict guidelines, requiring values close to zero, regarding the degradation of such pollutants in industrial streams. In many cases, classic bioremoval processes fail. Therefore, we proposed a stream leaving the microbial membrane bioreactor (MBR) that is directed to an additional membrane separation mode (NF/RO). Under certain conditions, the integrated process not only benefits the environment but may also increase the profitability of the bioreactor operation. An appropriate model was developed and tested in which the bioremoval of benzene and toluene by Pseudomonas fluorescens was used as an example. This paper presents equations for selecting the operation parameters of the integrated system to achieve the expected degree of industrial wastewater purification.

scholarly journals Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

2019 ◽  
Vol 8 (1) ◽  
pp. 50 ◽  
Author(s):  
Tanmoy Roy Tusher ◽  
Takuya Shimizu ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Ribosomal Rna ◽  
Industrial Wastewater ◽  
Microbial Consortium ◽  
Mineral Salt Medium ◽  
Intergenic Spacer ◽  
Mineral Salt ◽  
Microbial Consortia ◽  
Salt Medium ◽  
Bacterial Strains ◽  
Water Contaminant

Biodegradation of 1,4-dioxane, a water contaminant of emerging concern, has drawn substantial attention over the last two decades. A number of dioxane-degraders have been identified, though many of them are unable to metabolically utilize 1,4-dioxane. Moreover, it is considered more preferable to use microbial consortia rather than the pure strains, especially in conventional bioreactors for industrial wastewater treatment. In the present study, a stable 1,4-dioxane-degrading microbial consortium was enriched, namely 112, from industrial wastewater by nitrate mineral salt medium (NMSM). The consortium 112 is capable of utilizing 1,4-dioxane as a sole carbon and energy source, and can completely degrade 1,4-dioxane up to 100 mg/L. From the consortium 112, two 1,4-dioxane-degrading bacterial strains were isolated and identified, in which the Variovorax sp. TS13 was found to be a novel 1,4-dioxane-degrader that can utilize 100 mg/L of 1,4-dioxane. The efficacy of the consortium 112 was increased significantly when we cultured the consortium with mineral salt medium (MSM). The new consortium, N112, could utilize 1,4-dioxane at a rate of 1.67 mg/L·h. The results of the ribosomal RNA intergenic spacer analysis (RISA) depicted that changes in the microbial community structure of consortium 112 was the reason behind the improved degradation efficiency of consortium N112, which was exhibited as a stable and effective microbial consortium with a high potential for bioremediation of the dioxane-impacted sites and contaminated industrial wastewater.

scholarly journals Polychlorinated Biphenyl Rhizoremediation by Pseudomonas fluorescens F113 Derivatives, Using a Sinorhizobium meliloti nod System To Drive bph Gene Expression

2005 ◽  
Vol 71 (5) ◽  
pp. 2687-2694 ◽  
Author(s):  
Marta Villacieros ◽  
Clare Whelan ◽  
Martina Mackova ◽  
Jesper Molgaard ◽  
María Sánchez-Contreras ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pseudomonas Fluorescens ◽  
Sinorhizobium Meliloti ◽  
Polychlorinated Biphenyl ◽  
Expression System ◽  
Single Copy ◽  
Bacterial Strains ◽  
High Level Expression ◽  
High Level ◽  
Biodegradation Genes

ABSTRACT Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a derivative of F113 expressing polychlorinated biphenyl (PCB) biodegradation genes (F113pcb) has been reported previously, this strain shows a low level of bph gene expression, limiting its rhizoremediation potential. Here, a high-level expression system was designed from rhizobial nod gene regulatory relays. Nod promoters were tested in strain F113 by using β-galactosidase transcriptional fusions. This analysis showed that nodbox 4 from Sinorhizobium meliloti has a high level of expression in F113 that is dependent on an intact nodD1 gene. A transcriptional fusion of a nodbox cassette containing the nodD1 gene and nodbox 4 fused to a gfp gene was expressed in the alfalfa rhizosphere. The bph operon from Burkholderia sp. strain LB400 was cloned under the control of the nodbox cassette and was inserted as a single copy into the genome of F113, generating strain F113L::1180. This new genetically modified strain has a high level of BphC activity and grows on biphenyl as a sole carbon and energy source at a growth rate that is more than three times higher than that of F113pcb. Degradation of PCBs 3, 4, 5, 17, and 25 was also much faster in F113L::1180 than in F113pcb. Finally, the modified strain cometabolized PCB congeners present in Delor103 better than strain LB400, the donor of the bph genes used.

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