scholarly journals Serotype Distribution and Characteristics of the Minimum Inhibitory Concentrations of Streptococcus pneumoniae Isolated from Pediatric Patients in Kunming, China

2021 ◽  
Vol 78 (3) ◽  
pp. 954-960
Author(s):  
Mingbiao Ma ◽  
Mei Yuan ◽  
Ming Li ◽  
Xiaojuan Li ◽  
Hailin Huang ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pediatric Patients ◽  
Immunization Coverage ◽  
Serotype Distribution ◽  
Pneumococcal Infections ◽  
Minimum Inhibitory Concentrations ◽  
Polymerase Chain ◽  
Serotype Identification ◽  
Quellung Reaction ◽  
Resistance Rates

AbstractStreptococcus pneumoniae (S. pneumoniae) is the main conditional pathogen of acute respiratory infection in infants, children, and older adults worldwide. It was great significant to identify the epidemic characteristics of serotypes and antibiotic susceptibility for the prevention and treatment of S. pneumoniae diseases. This research assessed the serotype distribution and the minimum inhibitory concentrations (MICs) of S. pneumoniae isolated from pediatric patients to provide information on the epidemiology and antibiotic resistance of S. pneumoniae in Kunming, China. A total of 140 S. pneumoniae isolates were collected from pediatric patients at Kunming Children’s Hospital from January 2016 to October 2017. Serotype identification was done by Quellung reaction and multiplex polymerase chain reaction. MICs were determined by E-test. 140 isolates distributed in 13 types of serotypes. The top-three prevalent serotypes were 19F, 19A, and 6B. The immunization coverage rate of 13-valent pneumococcal conjugate vaccine (PCV) was relatively higher and should be introduced into the vaccination program in the region. MIC50 of penicillin, ceftriaxone, and levofloxacin was 1 μg/mL. MIC50 for meropenem and vancomycin was 0.38 μg/mL. MIC90 of penicillin, ceftriaxone, and levofloxacin was 1.5 μg/mL and that of meropenem and vancomycin was 0.5 μg/mL. The MIC90 of erythromycin was > 256 μg/mL. In summary, S. pneumoniae had low resistance rates to penicillin, ceftriaxone, levofloxacin, vancomycin, and meropenem, and these antibiotics could be the first-line agents for children with pneumococcal infections in Kunming.

scholarly journals Emergence of serotype 19A Streptococcus pneumoniae after PCV10 associated with a ST320 in adult population, in Porto Alegre, Brazil

2019 ◽  
Vol 147 ◽  
Author(s):  
M.P. Mott ◽  
J. Caierão ◽  
G.R. Cunha ◽  
M.M. Del Maschi ◽  
K. Pizzutti ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Adult Population ◽  
Porto Alegre ◽  
Post Vaccination ◽  
Vaccine Introduction ◽  
Invasive Diseases ◽  
Before And After ◽  
Polymerase Chain ◽  
Quellung Reaction ◽  
Selection Of

AbstractUse of pneumococcal conjugate vaccines has caused emergence of non-vaccine serotypes. No Brazilian data specifically about serotype 19A are available. We aimed to evaluate the frequency of occurrence, susceptibility profile and molecular epidemiology of serotype 19A before and after vaccine introduction in Brazil. Pneumococcal identification was performed by the conventional method. Strain serotype was determined by multiplex polymerase chain reaction (PCR) and/or Quellung reaction. Resistance was determined by Etest® and PCR was performed to determine the presence of macrolide resistance genes, ermB and/or mefA. Pneumococci were typed by Multilocus Sequence Typing. Thirty-eight serotype 19A Streptococcus pneumoniae were recovered, mostly from invasive diseases. Prevalence of serotype 19A increased following vaccination (from 3.5% before vaccination to 8.1% after, p = 0.04196). Non-susceptibility increased to most antimicrobials after vaccine introduction and was associated with clonal complex (CC)320. MLST showed nine different STs, which were grouped in one main CC: CC320 (63.9%). During the post-vaccination era, the frequency of this serotype increased significantly from 1.2% in 2011 to 18.5% in 2014 (p = 0.00001), with a concomitant decrease in the genetic variability: ST320 consistently predominated after vaccine-introduction (61.1%). Overall, our results showed a post-PCV10 increase in the frequency of serotype 19A. This was accompanied by a selection of CC320 and antimicrobial resistance.

scholarly journals Serotype distribution of Streptococcus pneumoniae isolated from children in Moscow before and after introduction of 13-valent pneumococcal conjugate vaccination

2020 ◽  
Vol 23 (3) ◽  
pp. 160-164
Author(s):  
Natalya M. Alyabyeva ◽  
Ekaterina A. Brzhozovskaya ◽  
Olga A. Ponomarenko ◽  
Anna V. Lazareva
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Serotype Distribution ◽  
Pneumococcal Infections ◽  
Immunization Program ◽  
Vaccine Serotypes ◽  
National Medical ◽  
Before And After ◽  
Inpatient And Outpatient Care ◽  
Conjugate Vaccination

Introduction. In 2014, a 13-valent conjugated pneumococcal vaccine (PCV13) was introduced into the children’s immunization program in Russia. In this regard, to describe and analyze the epidemiology of pneumococcal infections, it is important to study and evaluate the distribution of serotypes and the effect of PCV13 vaccination on the serotype distribution of the nasopharyngeal Streptococcus pneumoniae isolates isolated in children from 2010 to 2018. Materials and methods. The study included 708 nasopharyngeal pediatric pneumococcal isolates recovered from 2010 to 2018, in patients under 5 years of age, who received inpatient and outpatient care at the National Medical Research Center for Children’s Health (Moscow). Serotyping was performed using antisera and / or molecular typing by PCR Results. In total, 33 different serotypes were identified in the S. pneumoniae collection. Six predominant serotypes were accounted for 68.6% of the total distribution and included serotypes: 19F, 6B, 23F, 14, 15B/C, 6A. The average prevalence of vaccine serotypes was of 77.7% in 2010-2015, with a significant decrease to 52% in 2018, which was accompanied by an increase in the prevalence of serotype 15B/C (16% in 2018) and serotypes 11A and 23A, from 1.1% in 2010 to 9.3% and 8% respectively in 2018. Conclusion. The use of PCV13 vaccination in Russia has led to a significant decrease in the carriage of pneumococcal vaccine serotypes. These results emphasize the need for careful monitoring of an ever-changing pneumococcal population.

scholarly journals Serotype Distribution and Antimicrobial Resistance of Streptococcus pneumoniae Isolates from Pediatric Patients in Singapore

2000 ◽  
Vol 44 (8) ◽  
pp. 2193-2196 ◽  
Author(s):  
Shirlena Wee-Ling Soh ◽  
Chit Laa Poh ◽  
Raymond V. Tzer Pin Lin
Keyword(s):  
Multidrug Resistance ◽  
Streptococcus Pneumoniae ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Pediatric Patients ◽  
Pediatric Hospital ◽  
Serotype Distribution ◽  
Large Numbers ◽  
Susceptibility Patterns

ABSTRACT One hundred eighty Streptococcus pneumoniae strains isolated from children at a pediatric hospital in Singapore from 1997 to 1999 were serotyped and their antimicrobial susceptibility patterns were determined. Sixty-three percent of the isolates were resistant to penicillin. Significantly large numbers of the strains investigated were resistant to trimethoprim-sulfamethoxazole (87.8%), tetracycline (71.7%), erythromycin (67.8%), and chloramphenicol (40%). Penicillin and multidrug resistance was mostly associated with the frequently isolated S. pneumoniae isolates of serotypes (serotypes 19F, 23F, 6B, and 14). Isolates of serotype 19F, the serotype most commonly encountered in Singapore (41.1%), had the highest prevalence of penicillin (78.4%) and multidrug resistance (94.6%). Most of the invasive S. pneumoniae isolates (8 of 17; 47.1%) were of serotype 14.

scholarly journals The prevalence and clonal diversity of penicillin-resistant Streptococcus pneumoniae in Kuwait

2000 ◽  
Vol 125 (3) ◽  
pp. 573-581 ◽  
Author(s):  
K. AHMED ◽  
G. MARTINEZ ◽  
S. WILSON ◽  
R. YOSHIDA ◽  
R. DHAR ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clonal Diversity ◽  
Clinical Samples ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Common Serotypes ◽  
The Common ◽  
Polymerase Chain ◽  
Quellung Reaction

Penicillin-resistant Streptococcus pneumoniae (PRSP) is widespread all over the world, including countries previously free of PRSP. This study was undertaken to determine the prevalence, the common serotypes and the clonality of PRSP isolated over a period of 1 year, from various clinical samples from three major hospitals in Kuwait. Strains were identified by standard methods and their antibiotic susceptibility was determined by the agar dilution method. The clonality of the isolates was determined by repetitive extragenic palindromic sequence–polymerase chain reaction (REP–PCR) genomic profiling and pulsed field gel electrophoresis (PFGE). Serotyping was done by Quellung reaction using specific antisera. We found that 55% of the S. pneumoniae were resistant to penicillin (46% and 9% exhibited intermediate and full resistance, respectively). Nearly 41% were resistant to sulfamethoxazole-trimethoprim, 9% to cefotaxime and ceftriaxone, 15% to amoxycillin-clavulanate, 17% to cefuroxime, 77% to cefaclor, and 14% to clindamycin. The commonest serotypes among the PRSPs were 6A, 6B, 14, 19F, 23F and nontypable. PFGE and REP–PCR patterns showed a large diversity of genetic clones of the PRSP. Serotypes 6B, 14, 19F and 23F were more clonally related than the others. Our data showed that the prevalence of PRSP was high, the serotypes were diversified and different genetic clones make up the population of circulating PRSP in Kuwait.

Characterization of Streptococcus pneumoniae clones from paediatric patients with cystic fibrosis

2014 ◽  
Vol 63 (12) ◽  
pp. 1704-1715 ◽  
Author(s):  
Fernanda Pimentel de Araujo ◽  
Fabio D’Ambrosio ◽  
Romina Camilli ◽  
Ersilia Fiscarelli ◽  
Giovanni Di Bonaventura ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Streptococcus Pneumoniae ◽  
Conjugate Vaccine ◽  
Healthy Children ◽  
Large Variability ◽  
Biofilm Production ◽  
Typing Methods ◽  
Quellung Reaction ◽  
Resistance Rates

The role of Streptococcus pneumoniae in cystic fibrosis (CF) is poorly understood. The pneumococcal population has changed over time after the introduction of the heptavalent conjugate vaccine (PCV7) and, more recently, the 13-valent conjugate vaccine (PCV13). Although serotypes and clones causing invasive pneumococcal disease or colonizing healthy children have been extensively analysed, little is known so far on the serotypes and clones of pneumococci in CF patients. The aim of this work was to investigate serotypes, antibiotic susceptibilities, genotypes and biofilm production of CF pneumococcal isolates. Overall, 44 S. pneumoniae strains collected from 32 paediatric CF patients from January 2010 to May 2012 in a large Italian CF Centre were tested for antimicrobial susceptibility testing by Etest, serotyped by the Quellung reaction and genotyped by a combination of different molecular typing methods, including pbp gene restriction profiling, pspA restriction profiling and sequencing, PFGE and multilocus sequence typing. Biofilm production by pneumococcal strains was also assessed. Penicillin non-susceptibility was 16 %. High resistance rates (>56 %) were observed for erythromycin, clindamycin and tetracycline. The most frequent serotype recovered was serotype 3 (31.8 %). The coverage of PCV7 and PCV13 was 6.8 and 47.7 %, respectively. More than 80 % of CF strains belonged to Pneumococcal Molecular Epidemiology Network (PMEN) reference clones, the most common being Netherlands3-ST180 (28.2 %), and Greece21-30/ST193 (15.4 %). All strains produced biofilm in vitro, although with large variability in biofilm formation efficiency. No correlation was found between biofilm levels and serotype, clone or antibiotic resistance. The high isolation rate of antibiotic-resistant serotype 3 pneumococci from CF patients suggests that PCV13 could increase protection from pneumococcal colonization and infection.

scholarly journals Serotype Distribution, Antimicrobial Susceptibility, Multilocus Sequencing Type and Virulence of Invasive Streptococcus pneumoniae in China: A Six-Year Multicenter Study

2022 ◽  
Vol 12 ◽  
Author(s):  
Menglan Zhou ◽  
Ziran Wang ◽  
Li Zhang ◽  
Timothy Kudinha ◽  
Haoran An ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Multicenter Study ◽  
Antimicrobial Susceptibility ◽  
Capsular Polysaccharide ◽  
Microdilution Method ◽  
Strain Collection ◽  
Broth Microdilution Method ◽  
Quellung Reaction ◽  
Resistance Rates ◽  
Virulence Diversity

Background:Streptococcus pneumoniae is an important human pathogen that can cause severe invasive pneumococcal diseases (IPDs). The aim of this multicenter study was to investigate the serotype and sequence type (ST) distribution, antimicrobial susceptibility, and virulence of S. pneumoniae strains causing IPD in China.Methods: A total of 300 invasive S. pneumoniae isolates were included in this study. The serotype, ST, and antimicrobial susceptibility of the strains, were determined by the Quellung reaction, multi-locus sequence typing (MLST) and broth microdilution method, respectively. The virulence level of the strains in the most prevalent serotypes was evaluated by a mouse sepsis model, and the expression level of well-known virulence genes was measured by RT-PCR.Results: The most common serotypes in this study were 23F, 19A, 19F, 3, and 14. The serotype coverages of PCV7, PCV10, PCV13, and PPV23 vaccines on the strain collection were 42.3, 45.3, 73.3 and 79.3%, respectively. The most common STs were ST320, ST81, ST271, ST876, and ST3173. All strains were susceptible to ertapenem, levofloxacin, moxifloxacin, linezolid, and vancomycin, but a very high proportion (>95%) was resistant to macrolides and clindamycin. Based on the oral, meningitis and non-meningitis breakpoints, penicillin non-susceptible Streptococcus pneumoniae (PNSP) accounted for 67.7, 67.7 and 4.3% of the isolates, respectively. Serotype 3 strains were characterized by high virulence levels and low antimicrobial-resistance rates, while strains of serotypes 23F, 19F, 19A, and 14, exhibited low virulence and high resistance rates to antibiotics. Capsular polysaccharide and non-capsular virulence factors were collectively responsible for the virulence diversity of S. pneumoniae strains.Conclusion: Our study provides a comprehensive insight into the epidemiology and virulence diversity of S. pneumoniae strains causing IPD in China.

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