scholarly journals Serotype distribution of Streptococcus pneumoniae isolated from children in Moscow before and after introduction of 13-valent pneumococcal conjugate vaccination

2020 ◽  
Vol 23 (3) ◽  
pp. 160-164
Author(s):  
Natalya M. Alyabyeva ◽  
Ekaterina A. Brzhozovskaya ◽  
Olga A. Ponomarenko ◽  
Anna V. Lazareva
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Serotype Distribution ◽  
Pneumococcal Infections ◽  
Immunization Program ◽  
Vaccine Serotypes ◽  
National Medical ◽  
Before And After ◽  
Inpatient And Outpatient Care ◽  
Conjugate Vaccination

Introduction. In 2014, a 13-valent conjugated pneumococcal vaccine (PCV13) was introduced into the children’s immunization program in Russia. In this regard, to describe and analyze the epidemiology of pneumococcal infections, it is important to study and evaluate the distribution of serotypes and the effect of PCV13 vaccination on the serotype distribution of the nasopharyngeal Streptococcus pneumoniae isolates isolated in children from 2010 to 2018. Materials and methods. The study included 708 nasopharyngeal pediatric pneumococcal isolates recovered from 2010 to 2018, in patients under 5 years of age, who received inpatient and outpatient care at the National Medical Research Center for Children’s Health (Moscow). Serotyping was performed using antisera and / or molecular typing by PCR Results. In total, 33 different serotypes were identified in the S. pneumoniae collection. Six predominant serotypes were accounted for 68.6% of the total distribution and included serotypes: 19F, 6B, 23F, 14, 15B/C, 6A. The average prevalence of vaccine serotypes was of 77.7% in 2010-2015, with a significant decrease to 52% in 2018, which was accompanied by an increase in the prevalence of serotype 15B/C (16% in 2018) and serotypes 11A and 23A, from 1.1% in 2010 to 9.3% and 8% respectively in 2018. Conclusion. The use of PCV13 vaccination in Russia has led to a significant decrease in the carriage of pneumococcal vaccine serotypes. These results emphasize the need for careful monitoring of an ever-changing pneumococcal population.

Comparison of Incidence of Invasive Streptococcus pneumoniae Disease Among Children Before and After Introduction of Conjugated Pneumococcal Vaccine

2005 ◽  
Vol 24 (4) ◽  
pp. 320-323 ◽  
Author(s):  
Richard I. Haddy ◽  
Kelvin Perry ◽  
Chris E. Chacko ◽  
William B. Helton ◽  
Mark G. Bowling ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Vaccine ◽  
Before And After

Pneumococcal vaccine in adults reduces the risk of infections caused by Streptococcus pneumoniae

2016 ◽  
Vol 94 (1) ◽  
pp. 61-66
Author(s):  
Yulia G. Belocerkovskaja ◽  
A. G. Romanovskih ◽  
E. A. Styrt
Keyword(s):  
Streptococcus Pneumoniae ◽  
Conjugate Vaccine ◽  
Pneumococcal Vaccine ◽  
Pneumococcal Conjugate Vaccine ◽  
Risk Group ◽  
Severe Disease ◽  
Polysaccharide Vaccine ◽  
Pneumococcal Vaccines ◽  
Pneumococcal Polysaccharide Vaccine ◽  
Pneumococcal Infections

Streptococcus pneumoniae is a major cause of severe disease worldwide, particularly in the risk population. Two pneumococcal vaccines are currently available for specific prevention of pneumococcal infections among adults in Russia: a 23-valent pneumococcal polysaccharide vaccine (PPSV23) and a 13-valent pneumococcal conjugate vaccine (PCV13). The article describes modern views on the effectiveness and safety of two pneumococcal vaccines in adults with underlying medical conditions and adults aged ≥65 years and provides current recommendations for routine use of PPSV23 and PCV13 among persons included in the risk group.

scholarly journals Serotype Distribution and Characteristics of the Minimum Inhibitory Concentrations of Streptococcus pneumoniae Isolated from Pediatric Patients in Kunming, China

2021 ◽  
Vol 78 (3) ◽  
pp. 954-960
Author(s):  
Mingbiao Ma ◽  
Mei Yuan ◽  
Ming Li ◽  
Xiaojuan Li ◽  
Hailin Huang ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pediatric Patients ◽  
Immunization Coverage ◽  
Serotype Distribution ◽  
Pneumococcal Infections ◽  
Minimum Inhibitory Concentrations ◽  
Polymerase Chain ◽  
Serotype Identification ◽  
Quellung Reaction ◽  
Resistance Rates

AbstractStreptococcus pneumoniae (S. pneumoniae) is the main conditional pathogen of acute respiratory infection in infants, children, and older adults worldwide. It was great significant to identify the epidemic characteristics of serotypes and antibiotic susceptibility for the prevention and treatment of S. pneumoniae diseases. This research assessed the serotype distribution and the minimum inhibitory concentrations (MICs) of S. pneumoniae isolated from pediatric patients to provide information on the epidemiology and antibiotic resistance of S. pneumoniae in Kunming, China. A total of 140 S. pneumoniae isolates were collected from pediatric patients at Kunming Children’s Hospital from January 2016 to October 2017. Serotype identification was done by Quellung reaction and multiplex polymerase chain reaction. MICs were determined by E-test. 140 isolates distributed in 13 types of serotypes. The top-three prevalent serotypes were 19F, 19A, and 6B. The immunization coverage rate of 13-valent pneumococcal conjugate vaccine (PCV) was relatively higher and should be introduced into the vaccination program in the region. MIC50 of penicillin, ceftriaxone, and levofloxacin was 1 μg/mL. MIC50 for meropenem and vancomycin was 0.38 μg/mL. MIC90 of penicillin, ceftriaxone, and levofloxacin was 1.5 μg/mL and that of meropenem and vancomycin was 0.5 μg/mL. The MIC90 of erythromycin was > 256 μg/mL. In summary, S. pneumoniae had low resistance rates to penicillin, ceftriaxone, levofloxacin, vancomycin, and meropenem, and these antibiotics could be the first-line agents for children with pneumococcal infections in Kunming.

scholarly journals NASOPHARYNGEAL CARRIAGE OF STREPTOCOCCUS PNEUMONIAE IN CHILDREN UNDER 5 YEARS OF AGE AFTER INTRODUCTION OF PNEUMOCOCCAL CONJUGATE VACCINATION IN THE REPUBLIC OF KHAKASSIA

2019 ◽  
Vol 22 (4) ◽  
pp. 196-204
Author(s):  
A. R. Mirzaeva ◽  
T. V. Kulichenko ◽  
O. I. Lebedeva ◽  
Z. A. Alacheva ◽  
T. G. Kuznetsova ◽  
...  
Keyword(s):  
Vaccination Status ◽  
Nasopharyngeal Carriage ◽  
Pneumococcal Infections ◽  
Multicenter Cohort Study ◽  
Antibiotic Resistant ◽  
Vaccine Serotypes ◽  
Multicenter Cohort ◽  
The Republic ◽  
Conjugate Vaccination ◽  
Children Under 5

Introduction The dynamic study of the serotype composition and the level of antibiotic resistance of S. pneumoniae in different regions is the most important component of the control of pneumococcal infections (PI). The aim of the study was to analyze the serotype composition of S. pneumoniae isolated from the nasopharynx in children under 5 years of age, as well as to assess the sensitivity of pneumococci to antimicrobials, depending on the vaccination status and previous antibacterial therapy. Materials and methods A multicenter cohort study of nasopharyngeal carriage, serotype diversity and sensitivity to pneumococcal antibiotics were conducted in 13 centers in the Republic of Khakassia. Results 498 nasopharyngeal smears were collected, pneumococcus was isolated in 51.6% of cases. PI vaccination coverage in the cohort was 67.2%. The carriage of pneumococcus did not differ in children depending on their vaccination status (52.3% in vaccinated and 52.2% in unvaccinated). There was revealed a significant decrease in the carrier rate of pneumococcal vaccine serotypes in the cohort of vaccinated children compared with unvaccinated (17.6% vs 34.7%, p <0.05). High resistance of S. pneumoniae to penicillin (38.6%), macrolides (29.7%), trimethoprim/sulfamethoxazole (28.6%) was found. Amoxicillin resistance accounted for 20.8%, and ceftriaxone - 16.6%. Antibiotic-resistant vaccine serotypes were twofold more common. Conclusion The introduction of mass vaccination against PI in the country does not reduce the nasopharyngeal carriage of pneumococcus, but significantly reduces the circulation of vaccine serotypes, including strains resistant to antibiotics.

scholarly journals Changes in the pneumococcal vaccine serotypes in adult noninvasive pneumonia after the introduction of pneumococcal conjugate vaccination for children

2019 ◽  
Vol 11 (1) ◽  
pp. 30 ◽  
Author(s):  
Hiroaki Takeda ◽  
Chisa Sato ◽  
Chang Bin ◽  
Midori Nishidzuka ◽  
Mari Watanabe ◽  
...  
Keyword(s):  
Pneumococcal Vaccine ◽  
Vaccine Serotypes ◽  
Conjugate Vaccination

scholarly journals Genomic surveillance of invasive Streptococcus pneumoniae isolates in the period pre-PCV10 and post-PCV10 introduction in Brazil

2021 ◽  
Vol 7 (10) ◽  
Author(s):  
Samanta C. G. Almeida ◽  
Stephanie W. Lo ◽  
Paulina A. Hawkins ◽  
Rebecca A. Gladstone ◽  
Ana Paula Cassiolato ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
National Laboratory ◽  
Genetic Characteristics ◽  
Content Type ◽  
Vaccine Serotypes ◽  
Genetic Lineages ◽  
Immunization Programme ◽  
Before And After ◽  
Laboratory Surveillance ◽  
Group A

In 2010, Brazil introduced the 10-valent pneumococcal conjugate vaccine (PCV10) into the national children’s immunization programme. This study describes the genetic characteristics of invasive Streptococcus pneumoniae isolates before and after PCV10 introduction. A subset of 466 [pre-PCV10 (2008–2009): n=232, post-PCV10 (2012–2013): n=234;<5 years old: n=310, ≥5 years old: n=156] pneumococcal isolates, collected through national laboratory surveillance, were whole-genome sequenced (WGS) to determine serotype, pilus locus, antimicrobial resistance and genetic lineages. Following PCV10 introduction, in the <5 years age group, non-vaccine serotypes (NVT) serotype 3 and serotype 19A were the most frequent, and serotypes 12F, 8 and 9 N in the ≥5 years old group. The study identified 65 Global Pneumococcal Sequence Clusters (GPSCs): 49 (88 %) were GPSCs previously described and 16 (12 %) were Brazilian clusters. In total, 36 GPSCs (55 %) were NVT lineages, 18 (28 %) vaccine serotypes (VT) and 11 (17 %) were both VT and NVT lineages. In both sampling periods, the most frequent lineage was GPSC6 (CC156, serotypes 14/9V). In the <5 years old group, a decrease in penicillin (P=0.0123) and cotrimoxazole (P<0.0001) resistance and an increase in tetracycline (P=0.019) were observed. Penicillin nonsusceptibility was predicted in 40 % of the isolates; 127 PBP combinations were identified (51 predicted MIC≥0.125 mg l−1); cotrimoxazole (folA and/or folP alterations), macrolide (mef and/or ermB) and tetracycline (tetM, tetO or tetS/M) resistance were predicted in 63, 13 and 21.6 % of pneumococci studied, respectively. The main lineages associated with multidrug resistance in the post-PCV10 period were composed of NVT, GPSC1 (CC320, serotype 19A), and GPSC47 (ST386, serotype 6C). The study provides a baseline for future comparisons and identified important NVT lineages in the post-PCV10 period in Brazil.

scholarly journals Antibiotic resistance of Streptococcus pneumoniae isolated from children in Moscow before and after the introduction of 13-valent pneumococcal conjugate vaccination

2020 ◽  
Vol 23 (4) ◽  
pp. 216-222
Author(s):  
Natalia M. Alyabyeva ◽  
Ekaterina A. Brzhozovskaya ◽  
Olga A. Ponomarenko ◽  
Anna V. Lazareva ◽  
Andrey P. Fisenko
Keyword(s):  
Streptococcus Pneumoniae ◽  
Test Method ◽  
Diffusion Method ◽  
Pneumococcal Serotypes ◽  
Resistance Level ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
National Medical ◽  
Before And After ◽  
High Level

Introduction. The formation and spread of antibiotic-resistant Streptococcus pneumoniae isolates is a concern. After the thirteen-valent pneumococcal conjugate vaccine (PCV13) was added to the children’s immunization program in Russia in 2014, an important task is to monitor the resistance level to antimicrobials and the spectrum of circulating antibiotic-resistant pneumococcal serotypes. Materials and methods. The study included 708 nasopharyngeal pneumococcal isolates from children patients under 5 years who recovered from 2010 to 2018, received inpatient and outpatient care at the National Medical Research Center for Children’s Health (Moscow). The determination of sensitivity to antimicrobials was performed by the disk diffusion method. The MICs of penicillin, amoxicillin, and erythromycin were determined using the E-test method. Evaluation of the results was carried out according to the EUCAST-2019 criteria. Results. In total, 33 different serotypes were identified in the S. pneumoniae collection. Six predominant serotypes accounted for 68.6% of the total distribution and included serotypes: 19F, 6B, 23F, 14, 15B/C, 6A. In general, antimicrobial resistance ranged from 3.5% to chloramphenicol to 54.8% to tetracycline. The level of resistance to oxacillin, erythromycin, and clindamycin increased by 15-20% between 2010 and 2018. However, since 2016, there has established a prevalence level of 40-46%, this process is offset by an increase in the proportion of non-vaccine pneumococcal serotypes. Conclusion. A high level of resistance to penicillin and erythromycin remains. Monitoring the resistance level to antibiotics and the epidemiology of serotypes, clarifying the laws of formation of resistance, and its spread in a dynamically changing pneumococcal population is an important area of microbiology.

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