Asymbiotic in vitro seed propagation of Dendrobium

Nasturtium is a popular herbal plant, widely cultivated as culinary and medicinal plants all over the world. However, the seed propagation of nasturtium is inefficient, and in-vitro propagation is sophisticated and high-cost. In this study, the cutting propagation method was employed to produce nasturtium seedlings. We aimed to determine the optimal conditions for cutting propagation of nasturtium seedlings by investigating the effects of node position and electric conductivity (EC) of nutrient solution on the root formation of the cuttings. Cuttings from five node positions (apical bud, 2nd node, 3rd node, 4th node, and 5th node) were subjected to water and five EC (1.0, 2.0, 3.0, 4.0, and 5.0 dS m−1) treatments with a hydroponic cultivation system in a plant factory. Results showed that all cuttings rooted successfully within two weeks. The cuttings from the apical bud position rooted earliest and produced the most roots regardless of EC level. Cuttings from other node positions produced longer roots and heavier root fresh and dry weights than those from the apical bud position. The cuttings under EC of 1.0 dS m−1 had the greatest root number, the longest root length, and the heaviest root fresh and dry weights regardless of node positions. The EC of 1.0 dS m−1 is considered the best condition for nasturtium cuttings for the range of EC tested in this study, and the cuttings from all the five node positions can be used as seedling materials.

Download Full-text

Propagation of Juniper Species by Plant Tissue Culture: A Mini-Review

Forests ◽

10.3390/f10111028 ◽

2019 ◽

Vol 10 (11) ◽

pp. 1028 ◽

Cited By ~ 1

Author(s):

Hazubska-Przybył

Keyword(s):

In Vitro Culture ◽

Protective Action ◽

Ex Situ ◽

Conservation Strategies ◽

Culture Techniques ◽

Effective Strategies ◽

Dry Climates ◽

Coniferous Tree Species ◽

Seed Propagation

The genus Juniperus (of the Cupressaceae family) is the second most prevalent group of conifers on Earth. Juniper species are widely dispersed in the Northern Hemisphere, in Europe and Asia, and in Africa and Central America. Juniper species are resistant to dry climates and can adapt to difficult environmental conditions. Most juniper species are important in both ecological and economic terms. However, today, many forests in which junipers occur are being reduced in size due to both natural causes (fires, for example) and human activity (uncontrolled exploitation of forests, etc.). Also, climate changes may have adversely affected the range of populations of different juniper species. For this reason, some juniper species are now categorized as rare or endangered, and require immediate protective action. Therefore, there is an urgent need to develop effective strategies for ex situ conservation, including reliable procedures for Juniperus sp. reproduction for future reintroduction and restoration programs. The conservation strategies used until now with traditional forestry techniques (seed propagation, rooted cuttings, grafting) have not been satisfactory in many cases. Thus, increasing attention is being paid to the possibilities offered by in vitro culture technology, which enables the conservation and mass clonal propagation of different coniferous tree species. In this mini-review, we summarize the current state of knowledge regarding the use of various methods of the propagation of selected Juniperus species, with a particular emphasis on in vitro culture techniques.

Download Full-text

Symbiotic in vitro seed propagation of Dendrobium: fungal and bacterial partners and their influence on plant growth and development

Planta ◽

10.1007/s00425-015-2301-9 ◽

2015 ◽

Vol 242 (1) ◽

pp. 1-22 ◽

Cited By ~ 31

Author(s):

Jaime A. Teixeira da Silva ◽

Elena A. Tsavkelova ◽

Songjun Zeng ◽

Tzi Bun Ng ◽

S. Parthibhan ◽

...

Keyword(s):

Plant Growth ◽

Growth And Development ◽

Plant Growth And Development ◽

Seed Propagation

Download Full-text

ADAPTIVE CAPACITY OF SOME LAVENDER AND LAVANDIN CULTIVARSIN VITRO AND IN SITU

AGROFOR ◽

10.7251/agreng1701091g ◽

2018 ◽

Vol 2 (1) ◽

Cited By ~ 1

Author(s):

Oksana GREBENNIKOVA ◽

Anfisa PALIY ◽

Valentina BRAILKO ◽

Olga MITROFANOVA ◽

Valery RABOTYAGOV ◽

...

Keyword(s):

Phenolic Compounds ◽

Photosynthetic Activity ◽

Bound Water ◽

Leaf Tissue ◽

Viral Pathogens ◽

Intact Plants ◽

Seed Propagation ◽

Proline Concentration

Lavandula angustifolia Mill. and (LavandulaxintermediaEmericexLoisel) arepromising fragrant plants with medicinal, aromatic and ornamental properties.Since the collection plantations of these crops are very damaged with viralpathogens and there is lack of seed propagation in valuable cultivars 'Belyanka','Record' (lavender) and 'Rabat', 'Snezhnyi Bars' (lavandin), were introduced invitro. Chemotherapy was used for cleaning up. Regenerants were cultured (4-5months) on MS medium with 0. 3 mg L- Kinetin, 0. 025 mg L- NAA and 0. 25 mgL- GA3 at 25±1°C under 16-h photoperiod. Intact plants were studied during thegrowing season. In order to reveal plants` biotechnological and genetic capacitysome biochemical stress indicators, indexes of photosynthetic activity and waterregime were identified. Under the open field cultivation, tested plants were rich inascorbic acid, phenolic compounds, and redox enzymes (catalase, polyphenoloxidase, superoxide dismutase) were active. Leaf tissue hydration was 56-62%,with greater part of bound water. Photosynthetic activity was reduced only in thesamples with visible damages with viral pathogens. In plants cultured in vitro,amount of ascorbic acid and phenolic compounds were lower, so as enzymaticactivity and proline concentration were higher than in intact plants. The rate ofhydration was high (70-77%), with the same trend of water fractional composition.Photosynthetic activity and vitality index indicated no photoinhibition. It wasfound out the lavandin cultivars had better capacity for a wide use under variousculture conditions.

Download Full-text

Variability in the Chemical Composition of a New Aromatic Plant Artemisia balchanorum in Southern Russia

Plants ◽

10.3390/plants11010006 ◽

2021 ◽

Vol 11 (1) ◽

pp. 6

Author(s):

Gennadii V. Khodakov ◽

Lavr A. Kryukov ◽

Einat Shemesh-Mayer ◽

Rina Kamenetsky-Goldstein

Keyword(s):

Efficient Production ◽

Aromatic Plant ◽

Clonal Micropropagation ◽

Full Flowering ◽

Mass Reproduction ◽

Southern Russia ◽

Three Stages ◽

Seed Propagation ◽

Further Development

Lemon wormwood Artemisia balchanorum was recently introduced to southern Russia as a new aromatic plant. Based on biological and chemical characteristics, several populations with dominant citral, linalool, and geraniol production were selected for further development and maintained by seed propagation. Chemical analysis of five outstanding populations at three stages of annual development: vegetative, flower buds, and full flowering, confirmed that the seed populations retain the distinct dynamics of the dominant and minor components during the annual cycle and can be used for the commercial production of citral, linalool, and geraniol. Micropropagation in vitro allows for efficient clonal micropropagation and mass reproduction of elite cultivars and promising forms of A. balchanorum on a commercial scale but cannot serve as a source of direct and efficient production of secondary metabolites.

Download Full-text

In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

Agronomy ◽

10.3390/agronomy10050744 ◽

2020 ◽

Vol 10 (5) ◽

pp. 744

Author(s):

Samir C. Debnath ◽

Usha Arigundam

Keyword(s):

In Vitro Propagation ◽

Genetic Characteristics ◽

Current Review ◽

Small Fruit ◽

Antioxidant Metabolites ◽

Health Promoting ◽

Micropropagated Plants ◽

Conventional Methods ◽

Seed Propagation

Lingonberry (Vaccinium vitis-idaea L.) is a health-promoting small fruit crop rich in antioxidant metabolites that helps to reduce the incidence of degenerative diseases. Being heterozygous, lingonberries cannot preserve genetic characteristics through seed propagation. Conventional vegetative propagation, although it produces true-to-type plants, is not economically viable. In vitro propagation can multiply plants much faster than conventional methods. A liquid cultures system under a bioreactor micropropagation system is of significant importance to increase the multiplication rates of in vitro-produced shoots. Enhanced vegetative growth and variation in biochemical constituents are observed in micropropagated plants. Clonal fidelity, although it may be a serious problem for commercial micropropagation, can be verified efficiently by molecular markers. The current review provides detailed and updated information on lingonberry micropropagation along with conventional methods and their effects on morphological, molecular and biochemical characteristics in micropropagated plants, filling the gap in literature.

Download Full-text

Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson

Scientia Agricola ◽

10.1590/s0103-90162007000300008 ◽

2007 ◽

Vol 64 (3) ◽

pp. 263-268 ◽

Cited By ~ 14

Author(s):

Ronaldo Biondo ◽

Ana Valéria Souza ◽

Bianca Waléria Bertoni ◽

Andreimar Martins Soares ◽

Suzelei Castro França ◽

...

Keyword(s):

Medicinal Plant ◽

Plant Species ◽

In Vitro Propagation ◽

Nodal Segments ◽

Medicinal Plant Species ◽

Soil Conditions ◽

Ms Medium ◽

Germplasm Bank ◽

Seed Propagation

Mandevilla velutina (Mart.) Woodson (Apocynaceae) is a medicinal plant species with antivenom properties, native from Brazilian Savanna regions (Cerrado), which due to overexploitation and habitat deforestation is in danger of extinction. As an initiative for conserving this endangered but economically important plant species, a micropropagation protocol was developed and genotypes were stored in the Germplasm Bank "Cerrado In vitro". For the in vitro propagation of M. velutina, nodal segments were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of BA, Zeatin, 2ip, DTT and TDZ. Best multiplication ratio was achieved when to the medium 0.44 µM BA, ranging 1: 6.7, were added. Plantlets cultured on MS/2 medium supplemented with 26.85 µM NAA rooted successfully (50.5%). Although rooted and un-rooted plantlets acclimatized to soil conditions, great losses were observed within un-rooted plantlets, while the rooted presented 100 % survival. It was possible to maintain 43% of the M. velutina germplasm under healthy conditions for six months, with no subcultures, using the MS medium supplemented with 2% sucrose, 13.8 mM spermidine, 2% sorbitol and 2% dextrose.

Download Full-text

Can the Caper (Capparis spinosa L.) Still Be Considered a Difficult-to-Propagate Crop?

Horticulturae ◽

10.3390/horticulturae7090316 ◽

2021 ◽

Vol 7 (9) ◽

pp. 316

Author(s):

Francesco Sottile ◽

Chiara Caltagirone ◽

Cristiana Peano ◽

Maria Beatrice Del Signore ◽

Ettore Barone

Keyword(s):

Vegetative Propagation ◽

Genetic Erosion ◽

Horticultural Crop ◽

In Vitro Techniques ◽

Physiological Dormancy ◽

Critical Phase ◽

Capparis Spinosa ◽

Seed Propagation

As a perennial xerophytic shrub, characterized by plesiomorphic features, the caper (Capparis spinosa L.) is naturally spread throughout the Mediterranean basin and occupies an important ecological role, as well as an economic one, in traditional and specialized systems for commercial production. This species, in spite of its wide diffusion, is currently considered at risk of genetic erosion, mainly due to overgrazing and overharvesting for domestic uses and for trade. This situation is made more serious because of the lack of efficient propagation techniques, determining the caper as a “difficult-to-propagate species”. In this review, we report the main available sexual and vegetative propagation techniques with the aim of assessing whether, and to what extent, this criticality is still true for caper as a horticultural crop. In terms of seed propagation, germination rates have generally been considered quite low or unsatisfactory, and are also affected by hybridization phenomena that are likely to occur among both the wild and cultivated forms. The seeds show a physiological dormancy that can be lowered by adopting hormonal treatments, but in situ germination remains a critical phase. Vegetative propagation appears quite effective, mostly as related to in vitro techniques that allow caper cultivation that is no longer affected by propagation for an economic dissemination of the species in more intensive orchards. The research needs for Caper spinosa L. as a horticultural crop, especially in the field of genetic improvement and breeding, are also underlined.

Download Full-text