Formation and structural organization of the egg–sperm bundle of the scleractinian coral Montipora capitata

Coral Reefs ◽  
2010 ◽  
Vol 30 (2) ◽  
pp. 371-380 ◽  
Author(s):  
J. L. Padilla-Gamiño ◽  
T. M. Weatherby ◽  
R. G. Waller ◽  
R. D. Gates
Keyword(s):  
Scleractinian Coral ◽  
Structural Organization ◽  
Montipora Capitata ◽  
Sperm Bundle

Fragmentation and genotypic diversity of the scleractinian coral Montipora capitata in Kaneohe Bay, Hawaii

2008 ◽  
Vol 89 (1) ◽  
pp. 101-107 ◽  
Author(s):  
A. Nishikawa ◽  
R.A. Kinzie ◽  
K. Sakai
Keyword(s):  
Scleractinian Coral ◽  
Genotypic Diversity ◽  
The Other ◽  
Allozyme Electrophoresis ◽  
Clonal Structure ◽  
Montipora Capitata ◽  
Kaneohe Bay ◽  
Field Investigations ◽  
Sheltered Habitat ◽  
Reef Edge

The fragmentation and genotypic diversity of Montipora capitata was determined in Kaneohe Bay, Hawaii, using field investigations and allozyme electrophoresis. Two stations were established in the Bay, one in the centre (exposed reef edge, EXPO) and the other at the south end (sheltered lagoonal reef, SHEL). Although the number and mean per cent cover of attached colonies did not differ significantly between the two habitats, number and cover of unattached colonies (fragments) were significantly higher at the sheltered habitat. Thirty-seven genotypes were detected in 176 samples using two or three enzyme loci. Although mean genet number did not differ significantly between the two habitats (mean±SE, 8.2±1.2 and 12.2±1.7 in exposed and sheltered reefs, respectively), lower genetic diversity was detected at SHEL (mean NG:N±SE, 0.75±0.08 and 0.50±0.06 for EXPO and SHEL, respectively). There was no evidence of strong clonal structure, i.e. many colonies, but few genets. Sexually produced new genets may account for the high genotypic diversity in M. capitata at these two habitats.

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

2017 ◽  
Author(s):  
Hollie Putnam ◽  
Diane Adams ◽  
Ehud Zelzion ◽  
Nicole Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3319 ◽  
Author(s):  
Hollie M. Putnam ◽  
Diane K. Adams ◽  
Ehud Zelzion ◽  
Nicole E. Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra- and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2–3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

scholarly journals Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

2017 ◽  
Author(s):  
Hollie Putnam ◽  
Diane Adams ◽  
Ehud Zelzion ◽  
Nicole Wagner ◽  
Huan Qiu ◽  
...  
Keyword(s):  
Dna Markers ◽  
Sequence Variation ◽  
Marker Gene ◽  
Genomic Data ◽  
Single Copy ◽  
Rdna Repeat ◽  
Montipora Capitata ◽  
Sperm Bundle ◽  
Limited Variation ◽  
A Minor

We investigated intra and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

Ribosome Structural Organization

1974 ◽  
Vol 32 ◽  
pp. 332-333
Author(s):  
James A. Lake
Keyword(s):  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Small Subunit ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
Specific Antibodies ◽  
X Ray ◽  
E Coli ◽  
Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

Study of the Molecular Architecture of Keratin Filaments by Electron Microscopy

1982 ◽  
Vol 40 ◽  
pp. 118-119
Author(s):  
U. Aebi ◽  
P. Rew ◽  
T.-T. Sun
Keyword(s):  
Electron Microscopy ◽  
Structural Organization ◽  
Cell Types ◽  
Structural Features ◽  
Molecular Architecture ◽  
The Past ◽  
Keratin Filaments ◽  
Different Types ◽  
10 Nm Filaments ◽  
Different Cell Types

Various types of intermediate-sized (10-nm) filaments have been found and described in many different cell types during the past few years. Despite the differences in the chemical composition among the different types of filaments, they all yield common structural features: they are usually up to several microns long and have a diameter of 7 to 10 nm; there is evidence that they are made of several 2 to 3.5 nm wide protofilaments which are helically wound around each other; the secondary structure of the polypeptides constituting the filaments is rich in ∞-helix. However a detailed description of their structural organization is lacking to date.

Coral husbandry for ocean futures: leveraging abiotic factors to increase survivorship, growth, and resilience in juvenile Montipora capitata

2021 ◽  
Vol 657 ◽  
pp. 123-133
Author(s):  
JR Hancock ◽  
AR Barrows ◽  
TC Roome ◽  
AS Huffmyer ◽  
SB Matsuda ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Larval Rearing ◽  
Abiotic Conditions ◽  
Montipora Capitata ◽  
Reef Restoration ◽  
Juvenile Corals ◽  
Bleaching Response ◽  
The Face ◽  
Survival And Growth ◽  
Global And Local

Reef restoration via direct outplanting of sexually propagated juvenile corals is a key strategy in preserving coral reef ecosystem function in the face of global and local stressors (e.g. ocean warming). To advance our capacity to scale and maximize the efficiency of restoration initiatives, we examined how abiotic conditions (i.e. larval rearing temperature, substrate condition, light intensity, and flow rate) interact to enhance post-settlement survival and growth of sexually propagated juvenile Montipora capitata. Larvae were reared at 3 temperatures (high: 28.9°C, ambient: 27.2°C, low: 24.5°C) for 72 h during larval development, and were subsequently settled on aragonite plugs conditioned in seawater (1 or 10 wk) and raised in different light and flow regimes. These juvenile corals underwent a natural bleaching event in Kāne‘ohe Bay, O‘ahu, Hawai‘i (USA), in summer 2019, allowing us to opportunistically measure bleaching response in addition to survivorship and growth. This study demonstrates how leveraging light and flow can increase the survivorship and growth of juvenile M. capitata. In contrast, larval preconditioning and substrate conditioning had little overall effect on survivorship, growth, or bleaching response. Importantly, there was no optimal combination of abiotic conditions that maximized survival and growth in addition to bleaching tolerances. This study highlights the ability to tailor sexual reproduction for specific restoration goals by addressing knowledge gaps and incorporating practices that could improve resilience in propagated stocks.

RIBONUCLEOPROTEIN PARTICLES CONTAINING mRNA and pre-mRNA

1973 ◽  
Vol 74 (Suppl) ◽  
pp. S130-S167 ◽  
Author(s):  
O. P. Samarina ◽  
E. M. Lukanidin ◽  
G. P. Georgiev
Keyword(s):  
Structural Organization ◽  
General Scheme ◽  
Biological Significance ◽  
Mrna Transport ◽  
Ribonucleoprotein Particles ◽  
Mrna Precursor

ABSTRACT This paper is a review of the data concerning the nature, structural organization, properties and biological significance of the particles, containing mRNA and pre-mRNA (precursor of mRNA), i. e., (1) nuclear pre-mRNA-containing particles (2) free cytoplasmic mRNP (ribonucleoproteins), or informosomes (3) polysome-bound mRNP. Some new data on the comparison of nuclear and cytoplasmic particles, the nature of poly A-containing structures, involvement of informofers in Adenovirusspecific RNA transfer are presented. The general scheme of mRNA transport from nucleus to cytoplasm is discussed.

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