Exogenous Gamma-aminobutyric Acid Coordinates Active Oxygen and Amino Acid Homeostasis to Enhance Heat Tolerance in Wheat Seedlings

2021 ◽  
Author(s):  
Xing Wang ◽  
Xiaodong Wang ◽  
Chuanxi Peng ◽  
Hai Shi ◽  
Jia Yang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heat Tolerance ◽  
Active Oxygen ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Wheat Seedlings ◽  
Amino Acid Homeostasis

Purification of Antihemophilic Factor (Factor VIII) by Amino Acid Precipitation*

1964 ◽  
Vol 11 (01) ◽  
pp. 064-074 ◽  
Author(s):  
Robert H Wagner ◽  
William D McLester ◽  
Marion Smith ◽  
K. M Brinkhous
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Factor Viii ◽  
Plasma Protein ◽  
Acid Precipitation ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Specific Procedure ◽  
Beta Alanine ◽  
Antihemophilic Factor

Summary1. The use of several amino acids, glycine, alpha-aminobutyric acid, alanine, beta-alanine, and gamma-aminobutyric acid, as plasma protein precipitants is described.2. A specific procedure is detailed for the preparation of canine antihemophilic factor (AHF, Factor VIII) in which glycine, beta-alanine, and gammaaminobutyric acid serve as the protein precipitants.3. Preliminary results are reported for the precipitation of bovine and human AHF with amino acids.

Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets

2019 ◽  
Vol 10 (1) ◽  
pp. 366-378 ◽  
Author(s):  
Shuai Chen ◽  
Bie Tan ◽  
Yaoyao Xia ◽  
Simeng Liao ◽  
Meiwei Wang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Aminobutyric Acid ◽  
Gut Microflora ◽  
Gamma Aminobutyric Acid ◽  
Intestinal Immunity ◽  
Weaned Piglets ◽  
Amino Acid Profiles ◽  
Intestinal Functions ◽  
Weaning Piglets

This study aims to investigate the effects of dietary gamma-aminobutyric acid (GABA) supplementation on the growth performance, intestinal immunity, intestinal GABAergic system, amino acid profiles and gut microflora of the weaned piglets.

Brain amino acid concentrations during diving and acid-base stress in turtles

1985 ◽  
Vol 58 (6) ◽  
pp. 1751-1754 ◽  
Author(s):  
B. M. Hitzig ◽  
M. P. Kneussl ◽  
V. Shih ◽  
R. D. Brandstetter ◽  
H. Kazemi
Keyword(s):  
Amino Acid ◽  
Aminobutyric Acid ◽  
Breath Hold ◽  
Gamma Aminobutyric Acid ◽  
Amino Acid Neurotransmitters ◽  
Acid Base ◽  
Ventilatory Drive ◽  
Amino Acid Concentrations ◽  
Brain Amino Acid

To assess the role of brain amino acid neurotransmitters in the breath hold of diving animals, concentrations of free amino acids present in the brains of turtles immediately after 2 h of apneic diving (at 20 degrees C) were measured. Additionally, the same measurements were performed on four other groups of animals subjected to 2 h of hypercapnia (8% CO2 in air), anoxia (N2 breathing), anoxia plus hypercapnia (8% CO2–92% N2), or air breathing (control). Significant changes in the concentrations of the inhibitory amino acid neurotransmitters known to affect respiration [gamma-aminobutyric acid (GABA) and taurine] were seen. GABA increased significantly in those animals subjected to anoxia, whereas taurine decreased significantly in the diving animals and increased significantly in those subjected to anoxia plus hypercapnia. These results suggest that the attenuated central ventilatory drive during diving in these animals may be related to alterations in brain concentrations of GABA and taurine.

scholarly journals Techniques to optimize post-embedding single and double staining for amino acid neurotransmitters.

1992 ◽  
Vol 40 (7) ◽  
pp. 1011-1020 ◽  
Author(s):  
K D Phend ◽  
R J Weinberg ◽  
A Rustioni
Keyword(s):  
Electron Microscopy ◽  
Amino Acid ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Double Staining ◽  
Amino Acid Neurotransmitters ◽  
Simultaneous Visualization

We report a number of technical refinements for single and double staining with post-embedding electron microscopy for glutamate, aspartate, and gamma-aminobutyric acid. Best results were obtained with 2.5% glutaraldehyde in the fixative and by minimizing the duration of plastic polymerization and the interval between cutting and reacting. Quantitative documentation of the ability of exogenous glutamate, aspartate, and gamma-aminobutyric acid to block their immune staining is provided. Increased intensity of staining with the glutamate and aspartate antisera resulted from preincubation of glutamate antiserum with aspartate and aspartate antiserum with glutamate. To perform double staining with antisera raised in the same species, it was necessary to block antigenicity of the first antiserum; best results were obtained with hot paraformaldehyde fumes. By using a detergent instead of etching, these methods permitted the simultaneous visualization of tracers to identify neuroanatomic pathways.

scholarly journals Effects of some excitatory amino acid antagonists and drugs enhancing gamma-aminobutyric acid neurotransmission on pefloxacin-induced seizures in DBA/2 mice.

1997 ◽  
Vol 41 (2) ◽  
pp. 427-434 ◽  
Author(s):  
G De Sarro ◽  
F Nava ◽  
G Calapai ◽  
A De Sarro
Keyword(s):  
Amino Acid ◽  
Excitatory Amino Acid ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Nmda Receptor Complex ◽  
Amino Acid Antagonists ◽  
Quinolone Derivative ◽  
Sites Of Action ◽  
Anticonvulsant Activities ◽  
Potential Interactions

The behavioral and convulsant effects of pefloxacin (PEFLO), a quinolone derivative, were studied after intraperitoneal (i.p.) administration to Dilute Brown Agouti DBA/2J (DBA/2) mice, a strain genetically susceptible to sound-induced seizures. The anticonvulsant effects of some excitatory amino acid (EAA) antagonists acting at N-methyl-D-aspartate (NMDA) or alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) receptors and of some compounds enhancing gamma-aminobutyric acid (GABA)-ergic transmission against seizures induced by PEFLO were also evaluated. The present study demonstrated that both groups of compounds administered i.p. or intracerebroventricularly were able to protect against seizures induced by PEFLO. However, ifenprodil and (+/-)-alpha-(chlorophenyl)-4-[(4-fluorophenyl)methyl]-1-piperidine-ethan ol (SL 82.0715), two compounds acting on the polyamine site of the NMDA receptor complex, were unable to provide any protection. The relationship between the different sites of action and the anticonvulsant activities of these derivatives were discussed. Although the main mechanisms of PEFLO-induced seizures cannot be easily determined, potential interactions with the receptors of EAA exist. In fact, antagonists of EAA, and in particular, those acting at NMDA receptors, were able to increase the threshold for the seizures or to prevent the seizures induced by PEFLO, while compounds acting at the polyamine site did not provide any protection. The AMPA-KA receptor antagonists were also able to exert anticonvulsant activity, but with minor potency in comparison to those of NMDA antagonists. In addition, the fact that compounds enhancing GABA-ergic neurotransmission were also able to protect the mice against seizures induced by PEFLO suggests an involvement of GABA system.

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