Visual detection thresholds in the Asian honeybee, Apis cerana

In Pakistan, Apis cerana, the Asian honeybee, has been used for honey production and pollination services. However, its genomic makeup and phylogenetic relationship with those in other countries are still unknown. We collected A. cerana samples from the main cerana-keeping region in Pakistan and performed whole genome sequencing. A total of 28 Gb of Illumina shotgun reads were generated, which were used to assemble the genome. The obtained genome assembly had a total length of 214 Mb, with a GC content of 32.77%. The assembly had a scaffold N50 of 2.85 Mb and a BUSCO completeness score of 99%, suggesting a remarkably complete genome sequence for A. cerana in Pakistan. A MAKER pipeline was employed to annotate the genome sequence, and a total of 11,864 protein-coding genes were identified. Of them, 6750 genes were assigned at least one GO term, and 8813 genes were annotated with at least one protein domain. Genome-scale phylogeny analysis indicated an unexpectedly close relationship between A. cerana in Pakistan and those in China, suggesting a potential human introduction of the species between the two countries. Our results will facilitate the genetic improvement and conservation of A. cerana in Pakistan.

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Visual detection thresholds in two trophically distinct fishes are compromised in algal compared to sedimentary turbidity

Conservation Physiology ◽

10.1093/conphys/coy044 ◽

2018 ◽

Vol 6 (1) ◽

Cited By ~ 5

Author(s):

Chelsey L Nieman ◽

Andrew L Oppliger ◽

Caroline C McElwain ◽

Suzanne M Gray

Keyword(s):

Visual Detection ◽

Detection Thresholds

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Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies

Journal of Apicultural Science ◽

10.2478/jas-2019-0001 ◽

2019 ◽

Vol 63 (1) ◽

pp. 41-48 ◽

Cited By ~ 1

Author(s):

Asli Özkırım ◽

Aygün Schiesser ◽

Nevіn Keskin

Keyword(s):

Apis Mellifera ◽

Apis Cerana ◽

Winter Season ◽

Nosema Ceranae ◽

Molecular Techniques ◽

Winter Period ◽

Nosema Apis ◽

Infection Level ◽

Seasonal Infection ◽

Asian Honeybee

AbstractNosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.

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Response of foster Asian honeybee (Apis cerana Fabr.) colonies to the brood of European honeybee (Apis mellifera L.) infested with parasitic mite, Varroa jacobsoni oudemans

Journal of Invertebrate Pathology ◽

10.1016/0022-2011(87)90057-7 ◽

1987 ◽

Vol 49 (3) ◽

pp. 259-264 ◽

Cited By ~ 33

Author(s):

Ying-Shin Christine Peng ◽

Yuenzhen Fang ◽

Shaoyu Xu ◽

Lisheng Ge ◽

Medhat E. Nasr

Keyword(s):

Apis Mellifera ◽

Apis Cerana ◽

Varroa Jacobsoni ◽

Parasitic Mite ◽

Asian Honeybee ◽

European Honeybee

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Absence of Wolbachia in the workers of Asian honeybee, Apis cerana indica

International Journal of Life Sciences ◽

10.3126/ijls.v5i1.5230 ◽

2012 ◽

Vol 5 (1) ◽

pp. 19-24

Author(s):

Mahesh Pattabhiramaiah ◽

Dorothea Brueckner ◽

MS Reddy

Keyword(s):

Apis Cerana ◽

Life Sciences ◽

Present Communication ◽

Chain Reaction ◽

Reproductive Incompatibility ◽

Apis Cerana Indica ◽

Wolbachia Endosymbiont ◽

Polymerase Chain ◽

Asian Honeybee ◽

Different Parts

Wolbachia is a group of cytoplasmically inherited bacteria that can cause reproductive alterations in arthropods including parthenogenesis, reproductive incompatibility and feminization of genetic males. Wolbachia are found in a well studied group of insects, but there is a lack of data on their distribution. Workers of the honeybee sub species Apis cerana indica, collected from different parts of Karnataka, India were screened by PCR for Wolbachia, because this endosymbiont has been implicated in causing thelytoky in other Hymenoptera. In the present communication, we report the absence of Wolbachia endosymbiont in the workers of honeybee collected from different geographic locations of Karnataka using Wolbachia specific 16S rDNA polymerase chain reaction enzymes.DOI: http://dx.doi.org/10.3126/ijls.v5i1.5230 International Journal of Life Sciences Vol.5(1) 2011 19-24

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Gene reuse facilitates rapid radiation and independent adaptation to diverse habitats in the Asian honeybee

Science Advances ◽

10.1126/sciadv.abd3590 ◽

2020 ◽

Vol 6 (51) ◽

pp. eabd3590

Author(s):

Yongkun Ji ◽

Xingan Li ◽

Ting Ji ◽

Junbo Tang ◽

Lifei Qiu ◽

...

Keyword(s):

Apis Cerana ◽

Fine Tuning ◽

Ancestral Population ◽

Rapid Radiation ◽

Labor Division ◽

Complex Process ◽

Shared Ancestry ◽

Almost All ◽

Asian Honeybee

Animals with recent shared ancestry frequently adapt in parallel to new but similar habitats, a process often underlined by repeated selection of the same genes. Yet, in contrast, few examples have demonstrated the significance of gene reuse in colonization of multiple disparate habitats. By analyzing 343 genomes of the widespread Asian honeybee, Apis cerana, we showed that multiple peripheral subspecies radiated from a central ancestral population and adapted independently to diverse habitats. We found strong evidence of gene reuse in the Leucokinin receptor (Lkr), which was repeatedly selected in almost all peripheral subspecies. Differential expression and RNA interference knockdown revealed the role of Lkr in influencing foraging labor division, suggesting that Lkr facilitates collective tendency for pollen/nectar collection as an adaptation to floral changes. Our results suggest that honeybees may accommodate diverse floral shifts during rapid radiation through fine-tuning individual foraging tendency, a seemingly complex process accomplished by gene reuse.

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