First steps in the development of a liquid biopsy in situ hybridization protocol to determine circular RNA biomarkers in rat biofluids

2019 ◽  
Vol 35 (12) ◽  
pp. 1329-1338 ◽  
Author(s):  
Eimear Kirby ◽  
Wai Hei Tse ◽  
Daywin Patel ◽  
Richard Keijzer
Keyword(s):  
In Situ Hybridization ◽  
Liquid Biopsy ◽  
Circular Rna ◽  
Hybridization Protocol ◽  
Rna Biomarkers

scholarly journals Catalyzed Reported Deposition-Fluorescence In Situ Hybridization Protocol To Evaluate Phagotrophy in Mixotrophic Protists

2005 ◽  
Vol 71 (11) ◽  
pp. 7321-7326 ◽  
Author(s):  
Juan M. Medina-Sánchez ◽  
Marisol Felip ◽  
Emilio O. Casamayor
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Signal Amplification ◽  
Cell Attachment ◽  
Cell Loss ◽  
Alexa Fluor ◽  
Hybridization Protocol ◽  
Card Fish ◽  
First Time

ABSTRACT We describe a catalyzed reported deposition-fluorescence in situ hybridization (CARD-FISH) protocol particularly suited to assess the phagotrophy of mixotrophic protists on prokaryotes, since it maintains cell and plastid integrity, avoids cell loss and egestion of prey, and allows visualization of labeled prey against plastid autofluorescence. This protocol, which includes steps such as Lugol's-formaldehyde-thiosulfate fixation, agarose cell attachment, cell wall permeabilization with lysozyme plus achromopeptidase, and signal amplification with Alexa-Fluor 488, allowed us to detect almost 100% of planktonic prokaryotes (Bacteria and Archaea) and, for the first time, to show archaeal cells ingested by mixotrophic protists.

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

2009 ◽  
Vol 17 (8) ◽  
pp. 1041-1050 ◽  
Author(s):  
Kai Wang ◽  
Zaijie Yang ◽  
Changshen Shu ◽  
Jing Hu ◽  
Qiuyun Lin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tetraploid Cotton ◽  
Axial Resolution ◽  
Hybridization Protocol

Double-Network Interpenetrating Bone Cement via in situ Hybridization Protocol

2010 ◽  
Vol 20 (22) ◽  
pp. 3997-4011 ◽  
Author(s):  
Jing Wang ◽  
Changsheng Liu ◽  
Yufei Liu ◽  
Shuo Zhang
Keyword(s):  
In Situ Hybridization ◽  
Bone Cement ◽  
Double Network ◽  
Hybridization Protocol

A versatile fluorometric in situ hybridization method for the quantitation of hairpin conformations in DNA self-assembled monolayers

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4522-4531
Author(s):  
Jiale He ◽  
Xiaochen Hu ◽  
Xiaoyi Gao ◽  
Chenchen Meng ◽  
Yunchao Li ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Detection Performance ◽  
Self Assembled Monolayers ◽  
Optimal Detection ◽  
Hybridization Method ◽  
Assembled Monolayers ◽  
The Creation ◽  
Self Assembled ◽  
Hybridization Protocol

We report a versatile fluorometric in-situ hybridization protocol for quantifying hairpin conformations in DNA self-assembled monolayers on substrates, which facilitates the creation of hpDNA-based biosensors with optimal detection performance.

The Therapeutic Effect of Circular RNA ST3GAL6 on Blocking GC Malignant Behaviors Through Autophagy Regulated by the FOXP2/MET/Mtor Axis

2021 ◽  
Author(s):  
Penghui Xu ◽  
Xing Zhang ◽  
Jiacheng Cao ◽  
Jing Yang ◽  
Zetian Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Circular Rna ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
Induced Apoptosis

Abstract Background: Gastric cancer (GC) ranks third in motality among all cancers worldwide. Circular RNAs (circRNAs) play essential roles in the malignant progression and metastasis of gastric cancer. As a transcription factor, FOXP2 is involved in the progression of many tumours. However, the regulation and association between circRNAs and FOXP2 remain to be discovered. Methods: RNA sequencing was used to explore differential circRNA expression profile in gastric cancer and quantitative real-time PCR (qRT-PCR) were used to detect circST3GAL6 expression. The cellular location of circST3GAL6 was determined by fluorescence in situ hybridization (FISH). Functional experiments in circST3GAL6 knockdown and overexpression cell lines were performed in vitro and in vivo. The correlation between circST3GAL6 and miR-300 was confirmed by the RNA pull-down assay, dual-luciferase reporter assay and fluorescence in situ hybridization (FISH). The effects of circST3GAL6 on autophagy were detected by confocal microscopy and transmission electron microscopy (TEM). The mechanism of the circST3GAL6/miR-300/FOXP2 axis was verified by western blotting. The transcriptional regulation of Met by FOXP2 was proven by ChIP and luciferase reporter assays.Results: CircST3GAL6 was significantly depressed in GC tissues and cells. circST3GAL6 overexpression inhibited the proliferation, invasion and metastasis of GC cells in vitro and in vivo. Importantly, circST3GAL6 overexpression induced apoptosis and promote autophagy in GC cells. Furthermore, we found that circST3GAL6 sponged miR-300 and subsequently regulated FOXP2. We further revealed that FOXP2 suppressed the activation of the Met/AKT/mTOR axis, a classic pathway that regulates autophagy-mediated proliferation and migration.Conclusion: Our findings revealed that circST3GAL6 functions as a tumour suppressor through the miR-300/FOXP2 axis in GC, regulates apoptosis and autophagy through FOXP2-mediated transcriptional inhibition of the MET axis and may be a biomarker for GC treatment.

Sign in / Sign up

Export Citation Format

Share Document