Effects of physical and chemical treatments upon biophysical properties and micro-relief of human skin

2008 ◽  
Vol 300 (5) ◽  
pp. 243-251 ◽  
Author(s):  
Nicolas Atrux-Tallau ◽  
Ngoc T. T. Huynh ◽  
Laurie Gardette ◽  
Cyril Pailler-Mattéi ◽  
Hassan Zahouani ◽  
...  
Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 104
Author(s):  
Jennifer Pradelli ◽  
Fabiola Tuccia ◽  
Giorgia Giordani ◽  
Stefano Vanin

Diptera puparia may represent both in forensic and archaeo-funerary contexts the majority of the entomological evidence useful to reconstruct the peri and post-mortem events. Puparia identification is quite difficult due to the lack of identification keys and descriptions. In addition, external substances accumulated during the puparia permanence in the environment make the visualization of the few diagnostic characters difficult, resulting in a wrong identification. Six different techniques based on physical and chemical treatments have been tested for the removal of external substances from puparia to make identification at species level feasible. Furthermore, the effects of these methods on successful molecular analyses have also been tested as molecular identification is becoming an important tool to complement morphological identifications. The results of this study indicate that cleaning via warm water/soap, the sonication and treatment with a sodium hydroxide solution are the best methods to achieve a good quality of the samples.


Parasitology ◽  
2000 ◽  
Vol 121 (1) ◽  
pp. 49-54 ◽  
Author(s):  
A. BARTLETT ◽  
M. BROWN ◽  
C. MARRIOTT ◽  
P. J. WHITFIELD

Franz cells (2-chambered, air/fluid phase static diffusion devices, previously used for the study of drugs across viable human skin) are utilized for the first time to investigate the process of infection of human skin by Schistosoma mansoni cercariae. Skin obtained from cosmetic surgery sources was used in the Franz cells to describe the temporal dynamics of the early interaction of cercariae with skin. At 38 °C, about 50% of cercariae applied in water to the epidermal surface of the skin were irreversibly attached within 1 min and after 5 min about 85% were similarly irrecoverable. The technique also provides the means of following the early penetration path of cercariae by histological methods. Franz cell results on the dynamics of attachment/early penetration have been compared with those obtained using artificial skin equivalents and non-human mammalian skin models in the context of the physical and chemical differences between these systems and viable human skin. It is concluded that Franz cells provide a convenient system for directly investigating the early phases of S. mansoni cercariae interaction with human skin.


2015 ◽  
pp. 141-146 ◽  
Author(s):  
Z. Megías ◽  
A. Barrera ◽  
S. Manzano ◽  
C. Martínez ◽  
D. Garrido ◽  
...  

2003 ◽  
Vol 48 (2) ◽  
pp. 53-60 ◽  
Author(s):  
N.J. Cromar ◽  
H.J. Fallowfield

This paper reports the results of work to allow a more accurate enumeration, and gravimetric determination, of the relative proportions of algae and bacteria within the floccular matrix present in a High Rate Algal Pond (HRAP). Methodology involving a combination of physical and chemical treatments was used and the resulting material was separated using density gradient centrifugation. The “fractions” were analysed using microscopical image analysis. Each “fraction” was categorised as containing bacteria only, algae only or an association of bacteria and algae, the relative proportions of which were then determined by image analysis to enable a “true” gravimetric determination of the algal and bacterial components of HRAP biomass for the first time. The biomass from HRAPs operated both outdoors and in a glasshouse was examined over complete operational seasons to investigate environmental effects as well as possible effects of variation in COD loading rate and retention time on cell biovolumes and cell quotients. In this study, the accurate assessment of both algal and bacterial biomass, determined as cell volume or dry matter, in addition to measurement of carbon and nitrogen has enabled the calculation of cell quotients. This allows a direct comparison between these values obtained from a nutrient-rich system and those published values obtained from systems with various nutrient status. Conversion factors obtained may also be of value for deriving inputs for computer models for the design and operation of high rate algal ponds.


2017 ◽  
Vol 46 (41) ◽  
pp. 14382-14392 ◽  
Author(s):  
Subramanian Natarajan ◽  
S. Anantharaj ◽  
Rajesh J. Tayade ◽  
Hari C. Bajaj ◽  
Subrata Kundu

The recovery of spinel MnCo2O4 from spent LIBs was achieved by a set of physical and chemical treatments, their employment for water oxidation in alkaline conditions was studied, and it was found that the recovered spinel MnCo2O4 were more effective than recovered monometallic oxides.


2005 ◽  
Vol 122 (1-2) ◽  
pp. 109-121 ◽  
Author(s):  
H. Ben Salem ◽  
L. Saghrouni ◽  
A. Nefzaoui

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