scholarly journals A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography

2021 ◽  
Author(s):  
Liyuan Jiang ◽  
Yong Cao ◽  
Xianzhen Yin ◽  
Shuangfei Ni ◽  
Miao Li ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
High Resolution ◽  
Synchrotron Radiation ◽  
Neuronal Network ◽  
3D Imaging ◽  
3D Visualization ◽  
Micro Computed Tomography ◽  
Combinatorial Method ◽  
The Impact

AbstractExploring the three-dimensional (3D) morphology of neurons is essential to understanding spinal cord function and associated diseases comprehensively. However, 3D imaging of the neuronal network in the broad region of the spinal cord at cellular resolution remains a challenge in the field of neuroscience. In this study, to obtain high-resolution 3D imaging of a detailed neuronal network in the mass of the spinal cord, the combination of synchrotron radiation micro-computed tomography (SRμCT) and the Golgi-cox staining were used. We optimized the Golgi-Cox method (GCM) and developed a modified GCM (M-GCM), which improved background staining, reduced the number of artefacts, and diminished the impact of incomplete vasculature compared to the current GCM. Moreover, we achieved high-resolution 3D imaging of the detailed neuronal network in the spinal cord through the combination of SRμCT and M-GCM. Our results showed that the M-GCM increased the contrast between the neuronal structure and its surrounding extracellular matrix. Compared to the GCM, the M-GCM also diminished the impact of the artefacts and incomplete vasculature on the 3D image. Additionally, the 3D neuronal architecture was successfully quantified using a combination of SRμCT and M-GCM. The SRμCT was shown to be a valuable non-destructive tool for 3D visualization of the neuronal network in the broad 3D region of the spinal cord. Such a combinatorial method will, therefore, transform the presentation of Golgi staining from 2 to 3D, providing significant improvements in the 3D rendering of the neuronal network.

scholarly journals Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography

2021 ◽  
Author(s):  
Liyuan Jiang ◽  
Chengjun Li ◽  
Miao Li ◽  
Xianzhen Yin ◽  
Tianding Wu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Spinal Cord ◽  
Synchrotron Radiation ◽  
3D Visualization ◽  
Nerve Fibers ◽  
Micro Computed Tomography ◽  
Mouse Spinal Cord ◽  
Morphological Alterations ◽  
Neurovascular Structure ◽  
Broad Region

AbstractEffective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases. However, ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking. In this study, we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography (SRμCT) to visualize the 3D neurovascular network in the mouse spinal cord. Using our method, the 3D neurons, nerve fibers, and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning. Besides, we found that the 3D morphology of neurons, nerve fiber tracts, and vasculature visualized by SRμCT were highly consistent with that visualized using the histological method. Moreover, the 3D neurovascular structure could be quantitatively evaluated by the combined methodology. The method shown here will be useful in fundamental neuroscience studies.

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

Laser Use in Endodontic for Increase the Adhesion of Root Canal Filling. A synchrotron radiation micro tomography study

2018 ◽  
Vol 69 (8) ◽  
pp. 2144-2149
Author(s):  
Carmen Todea ◽  
Daliana Mocuta ◽  
Adrian Manescu ◽  
Gianfranco Semez ◽  
Alessandra Giuliani ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Synchrotron Radiation ◽  
Root Canal ◽  
Phase Contrast ◽  
Mechanical Treatment ◽  
Apical Region ◽  
Micro Computed Tomography ◽  
Root Canal Filling ◽  
Apical Area

The aim of the present paper is to explore, through a high-resolution synchrotron radiation-based micro computed tomography experiment, the efficiency of the Er:YAG laser involvement in the debridement of the root canal of pathologic teeth, applied after the chemo-mechanical treatment of the canal. The adhesion quality of root canal filling is the consequence of the root canal cleaning and shaping, thus preventing the leakage. Twelve extracted single-rooted teeth were considered. The first 5 mm of the roots, corresponding to the apical region of the teeth, were initially investigated through high-resolution synchrotron radiation phase-contrast micro computed tomography. Afterwards, a chemo-mechanical endodontic treatment was performed and the same region of the teeth was imaged again. Finally, an Er:YAG laser with PIPS tip treatment was applied on the same teeth and a last tomographic scan was done. The chemo-mechanical treatment properly removed the necrotic tissue only in the upper part of the apical area of root canal, but tissue debris were found in the lower and partially also in the medium and upper part of the apical region, especially when the apical area exhibited a larger curvature. The laser treatment applied afterwards gave good results in terms of removing the debris from the apical area of root canal, mainly when they remained in the main canal. The present study proves the capability of Er:YAG with PIPS tips laser treatment to enhance the removing of tissue debris from the apical region of root canals after a chemo-mechanical treatment. The high-resolution synchrotron radiation-based phase contrast micro computed tomography is the most powerful tool for a non-destructive 3D investigation method of the root canal morphology analysis and a proper characterization technique for the assessment of the endodontic treatment.

Advances in High-Resolution X-Ray Computed Tomography: Comparing the Latest in Cabinet-based Micro-Computed Tomography Technologies and Synchrotron Radiation-Based Tomography Beamlines

2012 ◽  
Vol 18 (S2) ◽  
pp. 576-577 ◽  
Author(s):  
R. Rudolph ◽  
A. Williams ◽  
O. Brunke
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Synchrotron Radiation ◽  
Micro Computed Tomography ◽  
X Ray ◽  
X Ray Computed

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

scholarly journals Optimizing imaging of the rat pulmonary microvasculature by micro-computed tomography

2019 ◽  
Vol 9 (4) ◽  
pp. 204589401988361
Author(s):  
Yupu Deng ◽  
Katelynn J. Rowe ◽  
Ketul R. Chaudhary ◽  
Anli Yang ◽  
Shirley H.J. Mei ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Pulmonary Arterial Hypertension ◽  
High Resolution ◽  
Arterial Hypertension ◽  
Pulmonary Vascular Disease ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Pulmonary Arterial ◽  
The Impact ◽  
Lung Microcirculation

Micro-computed tomography (micro-CT) is used in pre-clinical research to generate high-resolution three-dimensional (3D) images of organs and tissues. When combined with intravascular contrast agents, micro-CT can provide 3D visualization and quantification of vascular networks in many different organs. However, the lungs present a particular challenge for contrast perfusion due to the complexity and fragile nature of the lung microcirculation. The protocol described here has been optimized to achieve consistent lung perfusion of the microvasculature to vessels < 20 microns in both normal and pulmonary arterial hypertension rats. High-resolution 3D micro-CT imaging can be used to better visualize changes in 3D architecture of the lung microcirculation in pulmonary vascular disease and to assess the impact of therapeutic strategies on microvascular structure in animal models of pulmonary arterial hypertension.

scholarly journals In-situ analysis of the hydration ability of bone graft material using a synchrotron radiation X-ray micro-CT

2020 ◽  
Vol 18 ◽  
pp. 228080002096347
Author(s):  
Seung-Jun Seo ◽  
Yong-Gun Kim
Keyword(s):  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Bone Graft ◽  
3D Visualization ◽  
Calcium Phosphates ◽  
In Situ Analysis ◽  
Graft Material ◽  
Micro Computed Tomography ◽  
3D Information

The conventional micro-computed tomography (μCT) is a non-destructive imaging technique used for obtaining 2D and 3D information for scaffolds. The main composition and internal structure are important in mimicking and designing the characteristics of natural bone. This study was three-dimensional evaluating the external or internal structures and the hydration effects of bone graft materials by using the in-situ image technique. Synchrotron radiation micro-computed tomography (SR-μCT) was used to extract information on the geometry of two biphasic calcium phosphates (BCP) with identical chemicals and different micro-macro porosity, pore size distribution, and pore interconnection pathways. Volume analysis by hydration was used to measure the two bone graft materials at 0, 5, and 10-min intervals. The SR-μCT image was achieved with information regarding the internal pore structure and hydration effects evaluated under 3D visualization. Both types of bone graft materials showed structures suitable for tissue engineering applications. The SR-μCT in-situ techniques with 3D information provided a detailed view of the structures. Thus, SR-μCT could be an available, unbiased 3D alternative to in-situ analysis.

Cortical and trabecular bone parameters from HR-Pqct images at the Tibia: a local comparison with synchrotron radiation micro-computed tomography

2013 ◽  
Author(s):  
Agnes Ostertag ◽  
Francoise Peyrin ◽  
Sylvie Fernandez ◽  
Jean-Denis Laredo ◽  
Vernejoul Marie-Christine De ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Synchrotron Radiation ◽  
Trabecular Bone ◽  
Micro Computed Tomography ◽  
Bone Parameters ◽  
Local Comparison
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