Evaluation of different methods to determine total serum lipids for normalization of circulating organochlorine compounds

2009 ◽  
Vol 82 (10) ◽  
pp. 1241-1247 ◽  
Author(s):  
Roberto Bergonzi ◽  
Giuseppe De Palma ◽  
Cesare Tomasi ◽  
Maria Cristina Ricossa ◽  
Pietro Apostoli
Keyword(s):  
Serum Lipids ◽  
Organochlorine Compounds ◽  
Total Serum

GLYCOGEN DISEASE OF THE LIVER (VON GIERKE'S DISEASE) WITH HEPATOMATA

PEDIATRICS ◽  
1955 ◽  
Vol 16 (6) ◽  
pp. 785-800
Author(s):  
Howard H. Mason ◽  
Dorothy H. Andersen
Keyword(s):  
Lactic Acid ◽  
Blood Sugar ◽  
Terminal Illness ◽  
Serum Lipids ◽  
Right Heart Failure ◽  
Total Serum ◽  
Epinephrine Injection ◽  
Chemical Studies ◽  
Severity Of The Disease ◽  
Sugar Levels

A case of glycogen disease of the liver (von Gierke's disease), followed from the age of 6 months to death at 10½ years, is reported. The child was dwarfed and mentally retarded. The diagnosis is supported by blood sugar concentrations following the administration of monosaccharides and following epinephrine injection as well as by post-mortem anatomical and chemical studies. Hypoglycemia was controlled by frequent feedings which contained glucose or starch. Levulose and galactose were restricted because low tolerance tests suggested that these substances were rapidly removed from the blood by the liver; after phosphorylation and conversion in the liver their release as glucose was evidently blocked by the same mechanism that blocked the release of glucose from glycogen. Blood lactic acid was consistently elevated, the degree of elevation bearing an inverse relationship to blood sugar levels. It is suggested that excess blood lactic acid contributes to the acidosis and so therapy of the acidosis with lactates would be illogical. The concentration of total serum lipids was always high but fluctuated widely, reaching a peak of 7.45 gm./100 ml., higher during periods of prolonged hypoglycemia and probably reflecting the mobilization of body fat during these periods. The terminal illness was marked by 2 unexplained complications: multiple hepatomata and congestive right heart failure. The severity of the disease varies from patient to patient, in the present case appearing to decrease with advancing age.

scholarly journals Serum Level of Total Lipids and Telomere Length in the Male Population: A Cross-Sectional Study

2019 ◽  
Vol 13 (2) ◽  
pp. 155798831984297 ◽  
Author(s):  
Behrooz Karimi ◽  
Masud Yunesian ◽  
Ramin Nabizadeh ◽  
Parvin Mehdipour
Keyword(s):  
Serum Level ◽  
Telomere Length ◽  
Serum Lipids ◽  
Inverse Relationship ◽  
Repetitive Sequences ◽  
Initial Assessment ◽  
Total Serum ◽  
Cluster Sampling ◽  
Total Lipids ◽  
Age Related

Telomeres contain TTAGGG (T; Thymine, A; Adenine and G; Guanine) repetitive sequences and are placed at the end of human chromosomes. Telomere dysfunction is implicated in some age-related and chronic diseases, but its association with total serum lipids and obesity is unknown. Our objective was to determine influenced of total serum lipids on leukocyte telomere lengths (TLs). Participants were selected by cluster sampling from 22 districts of Tehran. The questionnaires were completed by 500 subjects and after the initial assessment in terms of lifestyle, nutrition, home, and job, 300 healthy people, aged 25–40 years were finally selected. TLs and serum level of total lipids were measured by quantitative real-time PCR and the Phillips method, respectively. The average telomere length (T/S) and total lipids were 1.05 ± 0.3 mg/dl and 643.3 ± 70.8 mg/dl, respectively. We found that a one unit difference in the following parameters were associated with kilo base pair differences in TL: Age −0.0002 (95% CI [−0.0022, −0.0018]), BMI −0.0019 (95% CI [−0.0003, −0.0034]), TC 0.0001 (95% CI [−0.0006, −0.0007]), TG −0.0010 (95% CI [−0.0015, −0.0004]), PL 0.0001 (95% CI [−0.0005, −0.0007]), and TSL −0.0003 (95% CI [−0.0008, 0.0001]). Spearman correlation analysis revealed an inverse relationship between TC (R = −0.53; 95% CI [−0.61, −0.44]), TG (R = −0.50; 95% CI [−0.58, −0.41]), PL (R = −0.46; 95% CI [−0.54−0.36]), and TSL (R = −0.63; 95% CI [−0.69, −0.56]) with T/S. Our research suggests that the inverse relationship was found between TL and weight, BMI, age, and TSL which were associated with obesity. High serum lipids concentration may be associated with systemic inflammation and atherosclerosis and may lead to oxidative stress, resulting in telomere shortening.

Polyphenol-Rich Phloem Enhances the Resistance of Total Serum Lipids to Oxidation in Men

2005 ◽  
Vol 53 (8) ◽  
pp. 3017-3022 ◽  
Author(s):  
Jaakko Mursu ◽  
Sari Voutilainen ◽  
Tarja Nurmi ◽  
Meri Helleranta ◽  
Tiina H. Rissanen ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Total Serum

Comparison of Total Serum Lipids Measured by Two Methods

1990 ◽  
Vol 10 (4) ◽  
pp. 468-472 ◽  
Author(s):  
James E. Heubi ◽  
Ronald J. Sokol ◽  
Catherine A. McGraw
Keyword(s):  
Serum Lipids ◽  
Total Serum

Chemical Basis of the Sulfo-phospho-vanillin Reaction for Estimating Total Serum Lipids

1972 ◽  
Vol 18 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Joseph A Knight ◽  
Shauna Anderson ◽  
James M Rawle
Keyword(s):  
Double Bond ◽  
Carbonyl Group ◽  
Serum Lipids ◽  
Gravimetric Method ◽  
Initial Step ◽  
Total Serum ◽  
Phosphate Ester ◽  
Unsaturated Lipids ◽  
Carbonium Ion ◽  
Concentrated Sulfuric Acid

Abstract Results with the "sulfo-phospho-vanillin" reaction, much used for determining total serum lipids, have been favorably compared with those for the gravimetric method. We investigated the basic chemistry of the reaction and determined the reactivity of this single reagent with various lipids. Our results suggest that: (a) The reaction requires a carbon-carbon double bond. (b) Concentrated sulfuric acid reacts with unsaturated lipids in the initial step to form a carbonium ion. (c) Phosphoric acid reacts with vanillin to produce a phosphate ester, with a resulting increase in the reactivity of the carbonyl group. (d) The carbonium ion reacts with the carbonyl group of phosphovanillin to form a colored compound, which is stabilized by resonance. (e) Unsaturated compounds with more than one double bond react, but reaction may vary with steric hindrance. (f) The method is reasonably precise, but its accuracy depends primarily on the reference standard used.

scholarly journals Effect of poloxamer 407 administration on the serum lipids profile, anxiety level and protease activity in the heart and liver of mice

2013 ◽  
Vol 6 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Tatyana Korolenko ◽  
Thomas P. Johnston ◽  
Nina I. Dubrovina ◽  
Yana A. Kisarova ◽  
Svetlana Ya. Zhanaeva ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Morphological Changes ◽  
Cathepsin L ◽  
Chronic Administration ◽  
Cysteine Proteases ◽  
Heart Tissue ◽  
Poloxamer 407 ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Early Stages

Abstract Chronic administration of the poloxamer 407 (P-407), a block copolymer, to elevate serum lipids in mice is a well-established mouse model of hyperlipidemia and atherosclerosis. We tested the hypothesis that the activity of several types of proteases in heart and liver tissue is changed in the early stages of atherosclerosis development. Additionally, we evaluated whether increased serum lipids would induce anxiety in mice, as determined by using a ‘plus-maze’ test. The mice were administered P-407 by intraperitoneal injection twice a week for one month. P-407 administration to mice resulted in a marked increase in total serum cholesterol, atherogenic non-HDLcholesterol, and especially in total triglycerides, and it also increased anxiety. Morphological changes observed in P-407-treated mice included contractile type changes in cardiomyocytes and foamy macrophages in liver. A significant increase of cysteine proteases cathepsin B and cathepsin L (at 24 h) and aspartate protease cathepsin D (at both 24 h and 5 days) was determined in heart tissue following P-407 administration. However, no changes were noted in heart matrix metalloproteinase activity. The activity of cysteine and aspartate proteases was significantly increased in liver at both 24 hours and 5 days after P-407 administration. In conclusion, administration of P-407 to mice for one month resulted in increased anxiety, and more importantly, there was an increase in the activity of heart and liver proteases secondary to sustained dyslipidemia. It is suggested that heart and liver cysteine and aspartate proteases may represent potential therapeutic targets in the early stages of atherosclerosis.

SERUM PROTEIN-BOUND IODINE AND SERUM LIPID CHANGES IN THE BABOON (PAPIO URSINUS)

1957 ◽  
Vol 14 (4) ◽  
pp. 309-316 ◽  
Author(s):  
A. VAN ZYL
Keyword(s):  
Menstrual Cycle ◽  
Serum Protein ◽  
Serum Lipids ◽  
Statistical Significance ◽  
Maximum Level ◽  
Reciprocal Relationship ◽  
Total Serum ◽  
Lipid Phosphorus ◽  
Papio Ursinus ◽  
Albumin Ratio

SUMMARY A study comprising 258 duplicate estimations of serum protein-bound iodine (PBI), total lipids, total cholesterol, lipid phosphorus and cholesterol/lipid phosphorus (C/P) ratios has been made in eleven adult female baboons during forty-one menstrual cycles. Comparison of these results with ninety-six corresponding estimations on thirteen adult male baboons observed over the same period indicates that: (a) The serum PBI and serum lipids in the female baboon fluctuate in rhythm with the phases of the menstrual cycle. Cyclical changes are not present in the male. (i) Serum PBI rises from the time of ovulation and reaches a peak just before perineal rest. (ii) Serum cholesterol rises from ovulation to the mid-point of deturgescence and drops rapidly afterwards to its initial level. (iii) Lipid phosphorus rises steadily during turgescence, reaching a maximum level after ovulation, and declines slowly to a minimal value at perineal rest and menstrual bleeding. (iv) The C/P ratio drops gradually during turgescence to ovulation and rises subsequently in deturgescence. (v) No rhythmic tendency of statistical significance was observed in total serum lipids. (b) The iodine/albumin ratio remains about constant during the entire cycle. (c) No reciprocal relationship was found between the PBI and cholesterol of any of the other serum fat fractions. The changes in serum PBI and serum lipids in the baboon's menstrual cycle differ from those described in the human female.

CHANGE OF TOTAL LIPIDS, TRIGLYCEROL, CHOLESTEROL AND GLUCOSE CNTENTIN THE BLOODOF LAYING HENS UNDER THE INFLUENCE OF NFNOAQVAHELATESOF SELENIUM, ZINC AND VITAMIN E

2019 ◽  
pp. 26-36
Author(s):  
M. Nishchemenko ◽  
V. Koziy ◽  
O. Omelchuk ◽  
A. Emelyanenko ◽  
O. Poroshinska ◽  
...  
Keyword(s):  
Vitamin E ◽  
Carbohydrate Metabolism ◽  
Serum Lipids ◽  
Laying Hens ◽  
Total Serum ◽  
Control Group ◽  
Total Lipids ◽  
Cholesterol Levels

The results of the study of the influence of selenium, zinc and vitamin E aqua chelates on lipid and carbohydrate metabolism in laying hens are reflected in the article. In the studies carried out on the layers of the breed, Lohman Brown examined the effect of the addition of selenium nanochemistry solutions at a dose of 30 ml / kg, zinc 30 ml / kg, along with vitamin E - 40 mg / kg of feed. The experiment lasted for 90 days, the use of the above-mentioned drugs caused changes in the content of the studied parameters, namely: a significant increase in the content of total serum lipids on the 60th and 90th day of the experiment in the experimental groups by 15.8-25.0% , and the level of triacylglycerol decreased in experimental layers by 12.8-13.3%. The study of cholesterol levels during the experiment revealed only a tendency to decrease within 4,7-6,1%. The concentration of glucose in the blood of the experimental layers during the experiment was significantly higher in the experiment by 7.2-8.4% compared to the control group of the layers.

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