Serum Level of Total Lipids and Telomere Length in the Male Population: A Cross-Sectional Study

Telomeres contain TTAGGG (T; Thymine, A; Adenine and G; Guanine) repetitive sequences and are placed at the end of human chromosomes. Telomere dysfunction is implicated in some age-related and chronic diseases, but its association with total serum lipids and obesity is unknown. Our objective was to determine influenced of total serum lipids on leukocyte telomere lengths (TLs). Participants were selected by cluster sampling from 22 districts of Tehran. The questionnaires were completed by 500 subjects and after the initial assessment in terms of lifestyle, nutrition, home, and job, 300 healthy people, aged 25–40 years were finally selected. TLs and serum level of total lipids were measured by quantitative real-time PCR and the Phillips method, respectively. The average telomere length (T/S) and total lipids were 1.05 ± 0.3 mg/dl and 643.3 ± 70.8 mg/dl, respectively. We found that a one unit difference in the following parameters were associated with kilo base pair differences in TL: Age −0.0002 (95% CI [−0.0022, −0.0018]), BMI −0.0019 (95% CI [−0.0003, −0.0034]), TC 0.0001 (95% CI [−0.0006, −0.0007]), TG −0.0010 (95% CI [−0.0015, −0.0004]), PL 0.0001 (95% CI [−0.0005, −0.0007]), and TSL −0.0003 (95% CI [−0.0008, 0.0001]). Spearman correlation analysis revealed an inverse relationship between TC (R = −0.53; 95% CI [−0.61, −0.44]), TG (R = −0.50; 95% CI [−0.58, −0.41]), PL (R = −0.46; 95% CI [−0.54−0.36]), and TSL (R = −0.63; 95% CI [−0.69, −0.56]) with T/S. Our research suggests that the inverse relationship was found between TL and weight, BMI, age, and TSL which were associated with obesity. High serum lipids concentration may be associated with systemic inflammation and atherosclerosis and may lead to oxidative stress, resulting in telomere shortening.

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CHANGE OF TOTAL LIPIDS, TRIGLYCEROL, CHOLESTEROL AND GLUCOSE CNTENTIN THE BLOODOF LAYING HENS UNDER THE INFLUENCE OF NFNOAQVAHELATESOF SELENIUM, ZINC AND VITAMIN E

Аграрний вісник Причорномор'я ◽

10.37000/abbsl.2019.94.05 ◽

2019 ◽

pp. 26-36

Author(s):

M. Nishchemenko ◽

V. Koziy ◽

O. Omelchuk ◽

A. Emelyanenko ◽

O. Poroshinska ◽

...

Keyword(s):

Vitamin E ◽

Carbohydrate Metabolism ◽

Serum Lipids ◽

Laying Hens ◽

Total Serum ◽

Control Group ◽

Total Lipids ◽

Cholesterol Levels

The results of the study of the influence of selenium, zinc and vitamin E aqua chelates on lipid and carbohydrate metabolism in laying hens are reflected in the article. In the studies carried out on the layers of the breed, Lohman Brown examined the effect of the addition of selenium nanochemistry solutions at a dose of 30 ml / kg, zinc 30 ml / kg, along with vitamin E - 40 mg / kg of feed. The experiment lasted for 90 days, the use of the above-mentioned drugs caused changes in the content of the studied parameters, namely: a significant increase in the content of total serum lipids on the 60th and 90th day of the experiment in the experimental groups by 15.8-25.0% , and the level of triacylglycerol decreased in experimental layers by 12.8-13.3%. The study of cholesterol levels during the experiment revealed only a tendency to decrease within 4,7-6,1%. The concentration of glucose in the blood of the experimental layers during the experiment was significantly higher in the experiment by 7.2-8.4% compared to the control group of the layers.

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The Use of Different Lipids to Express Serum Tocopherol: Lipid Ratios for the Measurement of Vitamin E Status

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328602300505 ◽

1986 ◽

Vol 23 (5) ◽

pp. 514-520 ◽

Cited By ~ 287

Author(s):

D I Thurnham ◽

J A Davies ◽

B J Crump ◽

R D Situnayake ◽

M Davis

Keyword(s):

Vitamin E ◽

Serum Lipids ◽

Total Serum ◽

Total Lipids ◽

Normal Range ◽

Vitamin E Deficiency ◽

Lipid Ratios ◽

Upper Limit ◽

Lipid Ratio ◽

Vitamin E Status

Plasma tocopherol was measured in 85 alcoholic patients and 40 control subjects from a local factory. Cholesterol, triglycerides and phospholipids were measured individually and summed to give an estimate of total serum lipids. Plasma tocopherol concentrations of the alchoholics were significantly lower than those of the controls and showed wide variation from marked deficiency to the upper limit of the normal range. Using regression analysis, 1·11 μmol tocopherol/mmol total lipids were calculated as the threshold of deficiency equivalent to 0·8 mg tocopherol/g total lipid established by Horwitt et al.1 The sensitivity and specificity of other tocopherol:lipid ratios for identifying vitamin E deficiency was compared with the tocopherol:total lipid ratio. Thresholds of deficiency for the different tocopherol:lipid ratios were calculated. The tocopherol:cholesterol+triglyceride ratio was found to be almost as powerful in identifying vitamin E deficiency as the tocopherol:total lipid ratio (sensitivity 95%, specificity 99%). Of the tocopherol:individual lipid ratios, the tocopherol:cholesterol ratio gave the best results (sensitivity 86%, specificity 94%).

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THE LIPOTROPIC ACTION OF COLD: 2. THE INFLUENCE OF COLD AND CHOLINE DEFICIENCY ON SERUM LIPIDS OF RATS AT DIFFERENT INTAKES OF DIETARY METHIONINE

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y65-046 ◽

1965 ◽

Vol 43 (3) ◽

pp. 451-456 ◽

Cited By ~ 2

Author(s):

M. W. Radomski ◽

J. D. Wood

Keyword(s):

Cold Exposure ◽

Serum Lipids ◽

Marked Decrease ◽

Total Serum ◽

Dietary Intakes ◽

Total Lipids ◽

Choline Deficiency ◽

Lipid Levels ◽

Cholesterol Levels ◽

Fatty Livers

The effect of exposure of rats to cold for a 10-day period on the concentration of serum lipids in control and choline-deficient animals was studied at four dietary intakes of methionine. Changes in total lipids, total and free cholesterol, phospholipid, and triglyceride levels were measured.In control (choline-fed) animals, phospholipid, cholesterol, and the ratio of free:ester cholesterol levels were not altered by cold exposure, but a marked decrease (25–30%, P < 0.01) in the levels of total serum lipids occurred. This was a result of a 45–70% decrease in triglyceride levels. The concentration of the triglyceride-rich low-density lipoproteins (Sf 10–400) in the serum fell in the cold-exposed animals as indicated by lowered triglyceride:cholesterol ratios.Although cold exposure prevented fatty livers in choline-deficient rats at high methionine intakes, it did not prevent the characteristic decrease in serum lipids found in choline deficiency at any of the methionine intakes studied. Decreases in total lipids (20–40%), triglyceride (10–50%), cholesterol (15–40%), and phospholipids (15–40%) were found. It was concluded that the ability of cold exposure to overcome choline deficiency is limited, apparently, to the fatty liver syndrome and has no effect on the decreased serum lipid levels.

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Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation

Mediators of Inflammation ◽

10.1155/2019/2324193 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Aaron L. Slusher ◽

Tiffany M. Zúñiga ◽

Edmund O. Acevedo

Keyword(s):

Gene Expression ◽

Young Adults ◽

Telomere Length ◽

Immune Cell ◽

Ex Vivo ◽

Middle Aged ◽

Human Telomerase Reverse Transcriptase ◽

Negatively Associated ◽

Htert Gene ◽

Age Related

Age-related elevations in proinflammatory cytokines, known as inflamm-aging, are associated with shorter immune cell telomere lengths. Purpose. This study examined the relationship of plasma PTX3 concentrations, a biomarker of appropriate immune function, with telomere length in 15 middle-aged (40-64 years) and 15 young adults (20-31 years). In addition, PBMCs were isolated from middle-aged and young adults to examine their capacity to express a key mechanistic component of telomere length maintenance, human telomerase reverse transcriptase (hTERT), following ex vivo cellular stimulation. Methods. Plasma PTX3 and inflammatory cytokines (i.e., IL-6, IL-10, TGF-β, and TNF-α), PBMC telomere lengths, and PBMC hTERT gene expression and inflammatory protein secretion following exposure to LPS, PTX3, and PTX3+LPS were measured. Results. Aging was accompanied by the accumulation of centrally located visceral adipose tissue, without changes in body weight and BMI, and alterations in the systemic inflammatory milieu (decreased plasma PTX3 and TGF-β; increased TNF-α (p≤0.050)). In addition, shorter telomere lengths in middle-aged compared to young adults (p=0.011) were negatively associated with age, body fat percentages, and plasma TNF-α (r=−0.404, p=0.027; r=−0.427, p=0.019; and r=−0.323, p=0.041, respectively). Finally, the capacity of PBMCs to increase hTERT gene expression following ex vivo stimulation was impaired in middle-aged compared to young adults (p=0.033) and negatively associated with telomere lengths (r=0.353, p=0.028). Conclusions. Proinflammation and the impaired hTERT gene expression capacity of PBMCs may contribute to age-related telomere attrition and disease.

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TERRA: A Novel Biomarker of Embryo Quality and Art Outcome

Genes ◽

10.3390/genes12040475 ◽

2021 ◽

Vol 12 (4) ◽

pp. 475

Author(s):

Maria Santa Rocca ◽

Ludovica Dusi ◽

Andrea Di Nisio ◽

Erminia Alviggi ◽

Benedetta Iussig ◽

...

Keyword(s):

Telomere Length ◽

Dna Sequences ◽

Male Fertility ◽

Embryo Quality ◽

Assisted Reproductive Technologies ◽

Biological Clock ◽

Reproductive Technologies ◽

Age Related ◽

Non Coding Rna ◽

First Time

Telomeres are considered to be an internal biological clock, and their progressive shortening has been associated with the risk of age-related diseases and reproductive alterations. Over recent years, an increasing number of studies have focused on the association between telomere length and fertility, identifying sperm telomere length (STL) as a novel biomarker of male fertility. Although typically considered to be repeated DNA sequences, telomeres have recently been shown to also include a long non-coding RNA (lncRNA) known as TERRA (telomeric repeat-containing RNAs). Interestingly, males with idiopathic infertility show reduced testicular TERRA expression, suggesting a link between TERRA and male fertility. The aim of this study was to investigate the role of seminal TERRA expression in embryo quality. To this end, STL and TERRA expression were quantified by Real Time qPCR in the semen of 35 men who underwent assisted reproductive technologies (ART) and 30 fertile men. We found that TERRA expression in semen and STL was reduced in patients that underwent ART (both p < 0.001). Interestingly, TERRA and STL expressions were positively correlated (p = 0.010), and TERRA expression was positively associated with embryo quality (p < 0.001). These preliminary findings suggest a role for TERRA in the maintenance of sperm telomere integrity during gametogenesis, and for the first time, TERRA expression was found as a predictive factor for embryo quality in the setting of assisted reproduction.

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A Comprehensive, Multidisciplinary, Personalized, Lifestyle Intervention Program is Associated with Increased Leukocyte Telomere Length in Children and Adolescents with Overweight and Obesity

Nutrients ◽

10.3390/nu13082682 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2682

Author(s):

George Paltoglou ◽

Christina Raftopoulou ◽

Nicolas C. Nicolaides ◽

Sofia M. Genitsaridi ◽

Sofia I. Karampatsou ◽

...

Keyword(s):

Children And Adolescents ◽

Telomere Length ◽

Lifestyle Intervention ◽

Intervention Program ◽

Overweight And Obesity ◽

Initial Assessment ◽

Biological Aging ◽

Childhood And Adolescence ◽

Lifestyle Interventions ◽

Lifestyle Intervention Program

Leucocyte telomere length (LTL) is a robust marker of biological aging and is associated with obesity and cardiometabolic risk factors in childhood and adolescence. We investigated the effect of a structured, comprehensive, multidisciplinary, personalized, lifestyle intervention program of healthy diet and physical exercise on LTL in 508 children and adolescents (239 males, 269 females; 282 prepubertal, 226 pubertal), aged 10.14 ± 0.13 years. Participants were classified as obese (n = 267, 52.6%), overweight (n = 174, 34.2%), or of normal BMI (n = 67, 13.2%) according to the International Obesity Task Force (IOTF) cutoff points and were studied prospectively for one year. We demonstrated that LTL increased significantly after 1 year of the lifestyle interventions, irrespective of gender, pubertal status, or body mass index (BMI). Waist circumference was the best negative predictor of LTL at initial assessment. The implementation of the lifestyle interventions also resulted in a significant improvement in clinical (BMI, BMI z-score and waist to height ratio) and body composition indices of obesity, inflammatory markers, hepatic enzymes, glycated hemoglobin (HbA1C), quantitative insulin sensitivity check index (QUICKI), and lipid profile in all participants. These findings indicate that the increased LTL may be associated with a more favorable metabolic profile and decreased morbidity later in life.

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Assessment of the Telomere Length and Its Effect on the Symptomatology of Parkinson’s Disease

Antioxidants ◽

10.3390/antiox10010137 ◽

2021 ◽

Vol 10 (1) ◽

pp. 137

Author(s):

Tina Levstek ◽

Sara Redenšek ◽

Maja Trošt ◽

Vita Dolžan ◽

Katarina Trebušak Podkrajšek

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Telomere Length ◽

Repetitive Sequences ◽

Housekeeping Gene ◽

Cellular Aging ◽

Brain Diseases ◽

Motor Complications ◽

Telomere Attrition ◽

Significant Difference

Telomeres, which are repetitive sequences that cap the end of the chromosomes, shorten with each cell division. Besides cellular aging, there are several other factors that influence telomere length (TL), in particular, oxidative stress and inflammation, which play an important role in the pathogenesis of neurodegenerative brain diseases including Parkinson’s disease (PD). So far, the majority of studies have not demonstrated a significant difference in TL between PD patients and healthy individuals. However, studies investigating the effect of TL on the symptomatology and disease progression of PD are scarce, and thus, warranted. We analyzed TL of peripheral blood cells in a sample of 204 PD patients without concomitant autoimmune diseases and analyzed its association with several PD related phenotypes. Monochrome multiplex quantitative PCR (mmqPCR) was used to determine relative TL given as a ratio of the amount of DNA between the telomere and albumin as the housekeeping gene. We found a significant difference in the relative TL between PD patients with and without dementia, where shorter TL presented higher risk for dementia (p = 0.024). However, the correlation was not significant after adjustment for clinical factors (p = 0.509). We found no correlations between TLs and the dose of dopaminergic therapy when the analysis was adjusted for genetic variability in inflammatory or oxidative factors. In addition, TL influenced time to onset of motor complications after levodopa treatment initiation (p = 0.0134), but the association did not remain significant after adjustment for age at inclusion and disease duration (p = 0.0781). Based on the results of our study we conclude that TL contributes to certain PD-related phenotypes, although it may not have a major role in directing the course of the disease. Nevertheless, this expends currently limited knowledge regarding the association of the telomere attrition and the disease severity or motor complications in Parkinson’s disease.

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Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Biology ◽

10.3390/biology10040253 ◽

2021 ◽

Vol 10 (4) ◽

pp. 253

Author(s):

Graciela Gavia-García ◽

Juana Rosado-Pérez ◽

Taide Laurita Arista-Ugalde ◽

Itzen Aguiñiga-Sánchez ◽

Edelmiro Santiago-Osorio ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Telomere Length ◽

Scientific Evidence ◽

Telomeric Dna ◽

Biochemical Alterations ◽

The Metabolic Syndrome ◽

Age Related ◽

And Function

A great amount of scientific evidence supports that Oxidative Stress (OxS) can contribute to telomeric attrition and also plays an important role in the development of certain age-related diseases, among them the metabolic syndrome (MetS), which is characterised by clinical and biochemical alterations such as obesity, dyslipidaemia, arterial hypertension, hyperglycaemia, and insulin resistance, all of which are considered as risk factors for type 2 diabetes mellitus (T2DM) and cardiovascular diseases, which are associated in turn with an increase of OxS. In this sense, we review scientific evidence that supports the association between OxS with telomere length (TL) dynamics and the relationship with MetS components in aging. It was analysed whether each MetS component affects the telomere length separately or if they all affect it together. Likewise, this review provides a summary of the structure and function of telomeres and telomerase, the mechanisms of telomeric DNA repair, how telomere length may influence the fate of cells or be linked to inflammation and the development of age-related diseases, and finally, how the lifestyles can affect telomere length.

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