Discovery of Paragonimus proliferus in Northern Vietnam and their molecular phylogenetic status among genus Paragonimus

Abstract A species of aphelenchoidid nematode was isolated from a subterranean termite, Cylindrotermes macrognathus, during a survey of termite-associated nematodes in a conserved forest in La Selva, Costa Rica. The nematode was morphologically intermediate between the families Aphelenchidae and Aphelenchoididae, i.e., the nematode had a true bursa supported by bursal limb-like genital papillae but lacked a clear pharyngeal isthmus. The molecular phylogenetic status of the new nematode among tylenchid, cephalobid, panagrolaimid, aphelenchid and aphelenchoidid genera was analysed based on ca 1.2 kb of SSU ribosomal DNA sequence and the inferred position was basal to the family Aphelenchoididae. It was clearly not part of the clade containing the genus Aphelenchus (=Aphelenchidae). This nematode is described herein as Pseudaphelenchus yukiae n. gen., n. sp., and the family definition of Aphelenchoididae is emended to include the unique morphological characters of this new genus. The molecular phylogenetic analysis supported the paraphyly of the three Aphelenchoidinae genera Aphelenchoides, Laimaphelenchus and Schistonchus and the monophyly of Ektaphelenchinae, Seinura (Seinurinae) and Noctuidonema (Acugutturinae). However, many more representatives are needed to resolve the family-genus level phylogeny of Aphelenchoididae.

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Molecular phylogenetic status of Siberian roe deer (Capreolus pygargus) based on mitochondrial cytochrome b from Jeju Island in Korea

Chinese Science Bulletin ◽

10.1007/s11434-014-0535-8 ◽

2014 ◽

Vol 59 (32) ◽

pp. 4283-4288 ◽

Cited By ~ 3

Author(s):

Yong-Su Park ◽

Baek-Jun Kim ◽

Woo-Shin Lee ◽

Jong-Taek Kim ◽

Tae-Wook Kim ◽

...

Keyword(s):

Cytochrome B ◽

Roe Deer ◽

Jeju Island ◽

Mitochondrial Cytochrome ◽

Siberian Roe Deer ◽

Mitochondrial Cytochrome B ◽

Phylogenetic Status ◽

Molecular Phylogenetic ◽

Capreolus Pygargus

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Modern views on phylogeny and systematic position of the genus Muscari (Asparagaceae) Miller

Biolohichni systemy ◽

10.31861/biosystems2020.02.312 ◽

2020 ◽

Vol 12 (2) ◽

pp. 312-318

Author(s):

Svitlana Boychuk ◽

Vasyl Budzhak

Keyword(s):

Phylogenetic Relationships ◽

Critical Analysis ◽

Molecular Data ◽

Systematic Position ◽

Modern Concept ◽

Angiosperm Phylogeny ◽

Phylogenetic Status ◽

The World ◽

Molecular Phylogenetic ◽

Plant Families

Systematic and phylogenetic status of Muscari – valuable ornamental and melliferous genus of flowering plants, still unclear despite numerous explorations. Therefore, the aim of the work was to carry out a critical analysis of publications devoted to phylogeny and taxonomy of the genus Muscari and to clarify its taxonomic position. The article provides a brief overview of the main publications dedicate to phylogeny and taxonomy of the genus. The systematic position of the genus Muscari in the early phylogenetic systems, taxonomic systems of the XX century and modern molecular phylogenetic systems is observed. Modern phylogenetic systems developed by Angiosperm Phylogeny Group (APG III, APG IV) and based on DNA sequence analysis. They show that Muscari belongs to the subfamily Scilloideae, family Asparagaceae s.l., order Asparagales, clusters Monocots. Phylogenetic relationships within the genus Muscari is difficult and controversial. In general, there are two main concepts of the genus Muscari division into subgenus. The first was developed by F. Speta in 1999 and is based on molecular data. In it, the genus is considered in a broad sense (s.l.), and divided into 4 subgenera: Muscari, Leopoldia, Muscarimia and Pseudomuscari. The second, more modern concept was developed by R. Govaerts and presented in the «World Checklist of selected plant families». According R. Govaerts, Muscari is divided into 3 separate genera: Muscari s.str. (=Muscarimia, Botryanthus), Leopoldia and Pseudomuscari. Paleobotanical data analysis established that the ancestral forms of modern Muscari originated approximately 3,29 million BC in the Mediterranean and spread to other regions of the world. The closest phylogenetic relatives of Muscari are the genera Scilla and Bellevalia. Chouardia and Nectaroscilla are also highly related. Comprehensive studies of morphological, biogeographical, molecular and other peculiarities of critical taxa are needed to clarification of the systematic position and phylogenetic relationships within the genus Muscari.

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Molecular phylogenetic relationships among Anatolian-Hyrcanian brown frog taxa (Ranidae: Rana)

Amphibia-Reptilia ◽

10.1163/15685381-00003114 ◽

2017 ◽

Vol 38 (3) ◽

pp. 339-350 ◽

Cited By ~ 3

Author(s):

Masoumeh Najibzadeh ◽

Michael Veith ◽

Ahmad Gharzi ◽

Nasrullah Rastegar-Pouyani ◽

Eskandar Rastegar-Pouyani ◽

...

Keyword(s):

Phylogenetic Relationship ◽

Dna Sequences ◽

Taxonomic Status ◽

Early Miocene ◽

Restricted Gene Flow ◽

Iranian Plateau ◽

Western Palearctic ◽

Phylogenetic Status ◽

Molecular Phylogenetic ◽

Relationship Of

Although the phylogenetic relationship of Western Palearctic brown frogs has been repeatedly studied, the taxonomic status and phylogenetic relationship of Anatolian-Hyrcanian brown frogs is still not fully resolved. Here, we assess the phylogenetic status of these species among Western Palearctic brown frogs with special emphasize on Iranian populations based on two partial mitochondrial DNA sequences (16S rRNA and cytochrome b genes) and the application of a molecular clock. Our results clearly show that Western Palearctic brown frogs underwent a basal radiation in to two main monophyletic clades, the European brown frogs plus the Asian R. asiatica and the Anatolian-Hyrcanian brown frogs, during Early Miocene ca. 20.2 mya. The Hyrcanian (R. pseudodalmatina) and the Anatolian lineage diverged approximately 16.6 mya. The further diverged into two subclades, R. tavasensis and R. macrocnemis, during the Middle Miocene, 14.5 mya. Our results suggest that diversification within these lineages may be closely linked to the formation of Neotethys and Paratethys and the subsequent uplift of the Turkish-Iranian plateau during the Early Miocene which led to restricted gene flow among brown frogs in these regions. Contrary to previous studies, we conclude that the Plio-Pleistocene epoch seems to be not associated to further significant speciation events within Anatolian-Hyrcanian brown frogs.

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Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽

10.1163/156854103322683283 ◽

2003 ◽

Vol 5 (4) ◽

pp. 565-572 ◽

Cited By ~ 19

Author(s):

Natsumi Kanzaki ◽

Kazuyoshi Futai

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Botrytis Cinerea ◽

Bursaphelenchus Xylophilus ◽

Lateral Field ◽

Female Tail ◽

Phylogenetic Status ◽

Rflp Profile ◽

Molecular Phylogenetic ◽

Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

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Discovery of Paragonimus skrjabini in Vietnam and its phylogenetic status in the Paragonimus skrjabini complex

Journal of Helminthology ◽

10.1017/s0022149x1200065x ◽

2012 ◽

Vol 87 (4) ◽

pp. 450-456 ◽

Cited By ~ 6

Author(s):

P.N. Doanh ◽

H.V. Hien ◽

N. Nonaka ◽

Y. Horii ◽

Y. Nawa

Keyword(s):

Species Complex ◽

Yunnan Province ◽

Asian Countries ◽

Cytochrome Oxidase Subunit ◽

Phylogenetic Studies ◽

Phylogenetic Status ◽

Molecular Phylogenetic ◽

Cytochrome Oxidase Subunit 1 ◽

China And Japan ◽

Paragonimus Skrjabini

AbstractTwo members of the Paragonimus skrjabini complex, P. skrjabini and P. miyazakii, are now considered as two sub-species, P. skrjabini skrjabini and P. skrjabini miyazakii. They are well known as important pathogens for human paragonimiasis in China and Japan. Recently, members of this species complex have been reported from India. Here we report the first discovery of P. skrjabini from freshwater crab hosts in Thanh Hoa province, Vietnam. For morphological and molecular phylogenetic studies, adult worms were obtained by experimental infection in cats and dogs. Molecular analyses of metacercariae and adults revealed that the P. skrjabini population from Thanh Hoa, Vietnam was almost completely identical with that from Yunnan province, China. Those populations from Thanh Hoa, Vietnam and Yunnan, China and those from Manipur, India were significantly different from P. skrjabini populations reported from other localities of China in cytochrome oxidase subunit 1 (CO1) gene sequences, indicating considerable genetic variation within the P. skrjabini complex. Moreover, low bootstrap values in the CO1 tree suggested that more variant genotypes belonging to P. skrjabini complex may be found in other Asian countries in between Vietnam and India, such as Myanmar, Laos and Thailand. Since P. skrjabini is known as a pathogen for humans, paragonimiasis cases caused by P. skrjabini might be found in Vietnam and other Asian countries.

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