Morphological and molecular identification of a lung fluke, Paragonimus macrorchis (Trematoda, Paragonimidae), found in central Lao PDR and its molecular phylogenetic status in the genus Paragonimus

2015 ◽  
Vol 64 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Oranuch Sanpool ◽  
Pewpan Maleewong Intapan ◽  
Tongjit Thanchomnang ◽  
Penchom Janwan ◽  
Sakhone Laymanivong ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Lao Pdr ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Lung Fluke

Discovery of Paragonimus proliferus in Northern Vietnam and their molecular phylogenetic status among genus Paragonimus

2007 ◽  
Vol 102 (4) ◽  
pp. 677-683 ◽  
Author(s):  
Pham Ngoc Doanh ◽  
Akio Shinohara ◽  
Yoichiro Horii ◽  
Shigehisa Habe ◽  
Yukifumi Nawa ◽  
...  
Keyword(s):  
Northern Vietnam ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic

Molecular identification and phylogenetic status of marineBacillus associated with coral sediment, showing antibacterial effects against human pathogens

2008 ◽  
Vol 58 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Shruti Chatterjee ◽  
Soumya Haldar ◽  
Masahiro Asakura ◽  
Shinji Yamasaki ◽  
Thangavel Balasubramanian
Keyword(s):  
Molecular Identification ◽  
Human Pathogens ◽  
Antibacterial Effects ◽  
Phylogenetic Status

Discovery of Paragonimus westermani in Vietnam and its molecular phylogenetic status in P. westermani complex

2008 ◽  
Vol 104 (5) ◽  
pp. 1149-1155 ◽  
Author(s):  
Pham Ngoc Doanh ◽  
Akio Shinohara ◽  
Yoichiro Horii ◽  
Shigehisa Habe ◽  
Yukifumi Nawa
Keyword(s):  
Paragonimus Westermani ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic

Copro-molecular identification of infections with hookworm eggs in rural Lao PDR

2010 ◽  
Vol 104 (9) ◽  
pp. 617-622 ◽  
Author(s):  
Megumi Sato ◽  
Surapol Sanguankiat ◽  
Tippayarat Yoonuan ◽  
Tiengkham Pongvongsa ◽  
Malaythong Keomoungkhoun ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Lao Pdr

Pseudaphelenchus yukiae n. gen., n. sp. (Tylenchina: Aphelenchoididae) associated with Cylindrotermes macrognathus (Termitidae: Termitinae) in La Selva, Costa Rica

Nematology ◽  
2009 ◽  
Vol 11 (6) ◽  
pp. 869-881 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Rudolf H. Scheffrahn ◽  
Barbara J. Center ◽  
Kerrie A. Davies
Keyword(s):  
Costa Rica ◽  
New Genus ◽  
Morphological Characters ◽  
Subterranean Termite ◽  
La Selva ◽  
The Family ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Definition Of ◽  
Ribosomal Dna Sequence

Abstract A species of aphelenchoidid nematode was isolated from a subterranean termite, Cylindrotermes macrognathus, during a survey of termite-associated nematodes in a conserved forest in La Selva, Costa Rica. The nematode was morphologically intermediate between the families Aphelenchidae and Aphelenchoididae, i.e., the nematode had a true bursa supported by bursal limb-like genital papillae but lacked a clear pharyngeal isthmus. The molecular phylogenetic status of the new nematode among tylenchid, cephalobid, panagrolaimid, aphelenchid and aphelenchoidid genera was analysed based on ca 1.2 kb of SSU ribosomal DNA sequence and the inferred position was basal to the family Aphelenchoididae. It was clearly not part of the clade containing the genus Aphelenchus (=Aphelenchidae). This nematode is described herein as Pseudaphelenchus yukiae n. gen., n. sp., and the family definition of Aphelenchoididae is emended to include the unique morphological characters of this new genus. The molecular phylogenetic analysis supported the paraphyly of the three Aphelenchoidinae genera Aphelenchoides, Laimaphelenchus and Schistonchus and the monophyly of Ektaphelenchinae, Seinura (Seinurinae) and Noctuidonema (Acugutturinae). However, many more representatives are needed to resolve the family-genus level phylogeny of Aphelenchoididae.

Molecular phylogenetic status of Siberian roe deer (Capreolus pygargus) based on mitochondrial cytochrome b from Jeju Island in Korea

2014 ◽  
Vol 59 (32) ◽  
pp. 4283-4288 ◽  
Author(s):  
Yong-Su Park ◽  
Baek-Jun Kim ◽  
Woo-Shin Lee ◽  
Jong-Taek Kim ◽  
Tae-Wook Kim ◽  
...  
Keyword(s):  
Cytochrome B ◽  
Roe Deer ◽  
Jeju Island ◽  
Mitochondrial Cytochrome ◽  
Siberian Roe Deer ◽  
Mitochondrial Cytochrome B ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Capreolus Pygargus

scholarly journals Ectomycorrhizae of Tomentella badia: description and molecular identification

2013 ◽  
Vol 48 (2) ◽  
pp. 155-171 ◽  
Author(s):  
Andrea Binder ◽  
Derek Peršoh ◽  
Nourou S. Yorou ◽  
Rita Verma ◽  
Claus Bässler ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Picea Abies ◽  
Molecular Identification ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Functional Characteristics ◽  
Molecular Phylogenetic ◽  
Dark Blue

Species within the genera <em>Tomentella </em>are among the most important ECM in forests. However, our knowledge about their functional characteristics is still rather limited. The ectomycorrhizae of <em>Tomentella badia </em>on <em>Picea abies </em>are described here in detail and compared to the non-identified ECM <em>Piceirhiza obscura</em>. A pseudoparenchymatous mantle formed by epidermoid cells is covered by heaps of epidermoid cells. This mantle type is regarded as a new one and designated as mantle type R. Many cells filled with dark blue contents and/or blue granules, together with clampless hyphae, are distinct characters of these ectomycorrhizae. Molecular-phylogenetic analysis of the ITS region was used for identification.

scholarly journals Phylogenomics and barcoding ofPanax: toward the identification of ginseng species

2018 ◽  
Author(s):  
V. Manzanilla ◽  
A. Kool ◽  
Nhat L. Nguyen ◽  
H. Nong Van ◽  
H. Le Thi Thu ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Molecular Identification ◽  
High Throughput Sequencing ◽  
Economic Value ◽  
Cost Effective ◽  
Morphological Characters ◽  
Species Discrimination ◽  
Sister Relationship ◽  
Plastid Genomes ◽  
Molecular Phylogenetic

AbstractBackgroundThe economic value of ginseng in the global medicinal plant trade is estimated to be in excess of US$2.1 billion. At the same time, the evolutionary placement of ginseng (Panax ginseng) and the complex evolutionary history of the genus is poorly understood despite several molecular phylogenetic studies. In this study, we use a full plastome phylogenomic framework to resolve relationships inPanaxand to identify molecular markers for species discrimination.ResultsWe used high-throughput sequencing of MBD2-Fc fractionatedPanaxDNA to supplement publicly available plastid genomes to create a phylogeny based on fully assembled and annotated plastid genomes from 60 accessions of 8 species. The plastome phylogeny based on a 163 kbp matrix resolves the sister relationship ofPanax ginsengwithP. quinquefolius. The closely related speciesP. vietnamensisis supported as sister ofP. japonicus. The plastome matrix also shows that the markerstrnC-rps16, trnS-trnG, andtrnE-trnMcould be used for unambiguous molecular identification of all the represented species in the genus.ConclusionsMBD2 depletion reduces the cost of plastome sequencing, which makes it a cost-effective alternative to Sanger sequencing based DNA barcoding for molecular identification. The plastome phylogeny provides a robust framework that can be used to study the evolution of morphological characters and biosynthesis pathways of ginsengosides for phylogenetic bioprospecting. Molecular identification of ginseng species is essential for authenticating ginseng in international trade and it provides an incentive for manufacturers to create authentic products with verified ingredients.

scholarly journals Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Enterobacter Cloacae ◽  
Selective Medium ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

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