Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

2012 ◽  
Vol 111 (4) ◽  
pp. 1645-1650 ◽  
Author(s):  
Jong-Hyun Kim ◽  
Jinyoung Lee ◽  
Hae-Jin Sohn ◽  
Hyun-Ok Song ◽  
Jung-Yeon Kim ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Sandwich Elisa

Lactate Dehydrogenase as Safe Endpoint Cooking Indicator in Poultry Breast Rolls: Development of Monoclonal Antibodies and Application to Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)

1993 ◽  
Vol 56 (2) ◽  
pp. 120-124 ◽  
Author(s):  
MOHAMED M. ABOUZIED ◽  
CHENG HSING WANG ◽  
JAMES J. PESTKA ◽  
DENISE M. SMITH
Keyword(s):  
Monoclonal Antibodies ◽  
Lactate Dehydrogenase ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Department Of Agriculture ◽  
Poultry Products ◽  
Chicken Muscle ◽  
Assay Detection

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect lactate dehydrogenase (LDH) as a marker protein for verifying endpoint cooking of uncured poultry products. Monoclonal antibodies were prepared against chicken muscle LDH and used with rabbit polyclonal antibodies developed against turkey or chicken muscle LDH for capture and detection in the assay, respectively. Minimum assay detection limits for turkey and chicken muscle LDH were 1 ng/ml. Turkey and chicken muscle LDH, but not LDH from other species cross reacted in the ELISA. The ELISA was further verified using extracts of turkey breast rolls processed to internal temperatures between 68.3 and 72.1°C. The LDH content of extracts diluted 3- to 6-fold was below 15 ng/ml for turkey rolls processed to 70.9 and 72.1°C. At a 6-fold dilution, LDH content of extracts from rolls processed to 69.7°C was approximately 10 times greater than those processed to 70.9°C. A survey of market precooked poultry products indicated assay validity with precooked turkey roast, but not turkey hams with maximum internal temperature requirements of 68.3°C. Results suggested the sandwich ELISA should be applicable for determining whether turkey breast rolls are processed to the required U.S. Department of Agriculture endpoint temperature of 71.1°C.

Lactate Dehydrogenase Monoclonal Antibody Sandwich ELISA To Determine Cooking Temperature of Ground Beef

1996 ◽  
Vol 44 (12) ◽  
pp. 4048-4051 ◽  
Author(s):  
A. Orta-Ramirez ◽  
C. H. Wang ◽  
M. M. Abouzied ◽  
G. J. Veeramuthu ◽  
J. F. Price ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Lactate Dehydrogenase ◽  
Sandwich Elisa ◽  
Ground Beef ◽  
Cooking Temperature

Preparation of monoclonal antibodies against KHV and establishment of an antigen sandwich ELISA for KHV detection

2019 ◽  
Vol 128 ◽  
pp. 36-40 ◽  
Author(s):  
Yingying Li ◽  
Qing Wang ◽  
Sven M. Bergmann ◽  
Weiwei Zeng ◽  
Yingying Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa

scholarly journals Polymorphism of the parasite lactate dehydrogenase gene from Plasmodium vivax Korean isolates

2013 ◽  
Vol 12 (1) ◽  
pp. 166 ◽  
Author(s):  
Hyun-Il Shin ◽  
Jung-Yeon Kim ◽  
Won-Ja Lee ◽  
Youngjoo Sohn ◽  
Sang-Wook Lee ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Dehydrogenase Gene

scholarly journals Missed Plasmodium falciparum and Plasmodium vivax Mixed Infections in Ethiopia Threaten Malaria Elimination

2021 ◽  
Author(s):  
Colleen M. Leonard ◽  
Hussein Mohammed ◽  
Mekonnen Tadesse ◽  
Jessica N. McCaffery ◽  
Doug Nace ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Therapeutic Efficacy ◽  
Dried Blood Spots ◽  
Mixed Infections ◽  
Efficacy Study ◽  
Chain Reaction ◽  
Polymerase Chain

Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia. This study investigated whether mixed infections were missed by microscopy from a 2017 therapeutic efficacy study at two health facilities in Ethiopia. All patients (N = 304) were initially classified as having single-species P. falciparum (n = 148 samples) or P. vivax infections (n = 156). Dried blood spots were tested for Plasmodium antigens by bead-based multiplex assay for pan-Plasmodium aldolase, pan-Plasmodium lactate dehydrogenase, P. vivax lactate dehydrogenase, and histidine-rich protein 2. Of 304 blood samples, 13 (4.3%) contained both P. falciparum and P. vivax antigens and were analyzed by polymerase chain reaction for species-specific DNA. Of these 13 samples, five were confirmed by polymerase chain reaction for P. falciparum/P. vivax co-infection. One sample, initially classified as P. vivax by microscopy, was found to only have Plasmodium ovale DNA. Plasmodium falciparum/P. vivax mixed infections can be missed by microscopy even in the context of a therapeutic efficacy study with multiple trained readers.

Rapid Detection of Salmonella spp. in Foods by Combination of a New Selective Enrichment and a Sandwich ELISA Using Two Monoclonal Antibodies against Dulcitol 1-Phosphate Dehydrogenase

1996 ◽  
Vol 59 (11) ◽  
pp. 1158-1163 ◽  
Author(s):  
HUAIZE TIAN ◽  
TAKAHISA MIYAMOTO ◽  
TAKASHI OKABE ◽  
YOICHIRO KURAMITSU ◽  
KEN-ICHI HONJOH ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Enrichment Culture ◽  
Sandwich Elisa ◽  
False Negative ◽  
Detection Sensitivity ◽  
Elisa Method ◽  
Salmonella Spp ◽  
Selective Enrichment ◽  
Raw Foods

A rapid-detection method was developed for food-borne dulcitol-positive Salmonella spp. in foods that involves a new preenrichment and selective enrichment system and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase. Preenrichment and selective enrichment were in Enterobacteriaceae enrichment mannitol (EEM) broth at 42°C for 6 h and in a new dulcitol-magnesium chloride-pyridinesulfonic acid brilliant green-novobiocin (DMPBN) medium at 42°C for 27 h, respectively. The cells were collected from the selective enrichment culture and suspended in 0.1 ml of 1 N NaOH for 2 min. The solution was neutralized with 0.1 ml of 2 M Tris-HCl buffer (pH 7.5) and the mixture was used as a sample for ELISA. The detection sensitivity of the ELISA was 105 CFU of Salmonella spp. per ml of culture. Competing non-Salmonella organisms in raw food did not interfere with the detection of Salmonella cells even when present at 107: 1 (non-Salmonella: Salmonella ratio) in food. Nonmotile Salmonella gallinarum was detected by the ELISA. The minimum detectable number of initial inoculum of Salmonella typhimurium was 0.69 CFU/25 g of raw chicken after the preenrichment in EEM broth and the selective enrichment in DMPBN medium. The present ELISA method required a total analysis time of 36 h including the preenrichment and selective enrichment periods. The ELISA method was compared with a conventional cultural method for the detection of Salmonella cells in 130 samples of raw foods. Of the samples tested, 16 were Salmonella-positive and 114 samples were negative by both methods. False-positive and false-negative results were not encountered.

Sign in / Sign up

Export Citation Format

Share Document