Development of a milk and serum ELISA test for the detection of Teladorsagia circumcincta antibodies in goats using experimentally and naturally infected animals

2014 ◽  
Vol 113 (10) ◽  
pp. 3651-3660 ◽  
Author(s):  
Eleni Malama ◽  
Peggy Hoffmann-Köhler ◽  
Insa Biedermann ◽  
Regine Koopmann ◽  
Jürgen Krücken ◽  
...  
Keyword(s):  
Elisa Test ◽  
Teladorsagia Circumcincta ◽  
Serum Elisa

scholarly journals Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Abel Villa-Mancera ◽  
Pedro Molina-Mendoza ◽  
Karina Hernández-Guzmán ◽  
Jaime Olivares-Pérez ◽  
Jorge Sarracent-Pérez ◽  
...  
Keyword(s):  
Serological Test ◽  
Sandwich Elisa ◽  
Correlation Coefficients ◽  
Economic Loss ◽  
Elisa Test ◽  
Current Status ◽  
Antigen Elisa ◽  
Faecal Samples ◽  
Diagnostic Sensitivity And Specificity ◽  
Serum Elisa

The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P<0.01) for low seropositivity (r=0.93) and medium seropositivity (r=0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status ofF. hepaticainfection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

scholarly journals Severe necrotizing myelopathy from toxacariasis

2015 ◽  
Vol 42 (S1) ◽  
pp. S29-S29
Author(s):  
R Magun ◽  
JE Silva
Keyword(s):  
Cervical Spinal Cord ◽  
Transverse Myelitis ◽  
Elisa Test ◽  
Minimal Improvement ◽  
Oligoclonal Banding ◽  
Leg Weakness ◽  
Necrotizing Myelopathy ◽  
Serum Elisa ◽  
Post Infectious ◽  
Spinal Cord Edema

We present a 25 year old female veterinarian technician presenting with rapidly progressive quadriplegia in less then 12 hours. Her symptoms occurred at work with initially bilateral hand weakness followed by arm and leg weakness. Before the end of the day she was on a ventilator in the ICU. MRI showed a hyperintense longitudinal T2 signal extending from the cervical medullary junction to T1. Extensive cervical spinal cord edema with cord expansion was noted. CSF showed normal protein and cell count with no oligoclonal banding. A post-infectious inflammatory process causing transverse myelitis was presumed and she was given IVIG, steroids, and plasmapheresis with no improvement. A serum ELISA test for IgG to Toxocara was reactive at titre of 1:800 at 3 weeks after her initial presentation. Her serum IgE levels was elevated at 169 x 10x3 U/L (Normal <87 x 10x3 U/L). At 4 weeks, she was commenced on albendazole at 800 mg per day for two months. A repeat serum ELISA test at 6 weeks and 2 weeks into her treatment with albendazole showed a declining titre of 1:200 consistent with recent Toxocara infection. At 10 weeks, her ELISA test was non-reactive. Unfortunately she did not respond to albendazole treatment and she shows minimal improvement now 1.5 years later.

Preliminary study on the use of a Teladorsagia circumcincta bulk milk ELISA test in dairy goats under experimental conditions

2009 ◽  
Vol 166 (3-4) ◽  
pp. 228-234 ◽  
Author(s):  
J.M. Molina ◽  
Y. Hernández ◽  
A. Ruiz ◽  
J.F. González ◽  
A. Argüello ◽  
...  
Keyword(s):  
Elisa Test ◽  
Dairy Goats ◽  
Experimental Conditions ◽  
Teladorsagia Circumcincta ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Preliminary Study ◽  
Bulk Milk Elisa

scholarly journals Ist die Expression des GP88-Proteins (Progranulin) ein klinisch relevanter Prognosefaktor für Prostatakarzinompatienten?

2021 ◽  
Vol 28 (1) ◽  
pp. 21-24
Author(s):  
Helge Taubert ◽  
Markus Eckstein
Keyword(s):  
Liquid Biopsy ◽  
Elisa Test ◽  
Test Liquid ◽  
Serum Elisa

ZusammenfassungProgranulin (GP88), ein autokriner Wachstumsfaktor, stellt einen für zahlreiche Tumorentitäten vielversprechenden Biomarker dar. Da sich GP88 sowohl im Tumorgewebe als auch im Serum von Tumorpatienten nachweisen lässt, ist ein minimal-invasiver Test („liquid biopsy“) zum Nachweis von GP88 möglich. Im Prostatakarzinom (PCa) wurde dieser Marker bisher nur in wenigen Voruntersuchungen auf seine diagnostische Aussagekraft hin charakterisiert. In unseren eigenen Arbeiten analysierten wir die Proteinlevel von GP88 im Serum (ELISA-Test) und im Tumorgewebe (Immunhistochemie) in 2 Prostatakarzinompatientenkohorten. Dabei erwies sich der verstärkte Proteinnachweis sowohl im Serum als auch im Tumorgewebe als negativer Prognosefaktor. Interessanterweise traf dies nur auf die jüngeren PCa-Patienten zu. Es sind weitere Untersuchungen erforderlich, um diese Ergebnisse zu bestätigen bzw. eine Eignung von GP88 auch für die Diagnose und das Therapiemonitoring von PCa-Patienten einzuschätzen.

Automated Latex Agglutination and ELISA Testing Yield Equivalent D-Dimer Results in Patients with Recent Myocardial Infarction

1999 ◽  
Vol 82 (11) ◽  
pp. 1412-1416 ◽  
Author(s):  
Wojciech Zareba ◽  
John Horan ◽  
Arthur Moss ◽  
Joel Kanouse ◽  
◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Elisa Test ◽  
Mean Value ◽  
Latex Agglutination ◽  
Test Results ◽  
Coronary Death ◽  
Elisa Testing ◽  
Strong Linear Correlation ◽  
Highly Correlated ◽  
D Dimer

SummaryOur previous prospective study of post-infarction patients described a strong and significant association of increased plasma D-dimer concentrations in those who experienced a subsequent coronary death or non-fatal myocardial infarction. In the present study, we compare results on stored plasma obtained two months after the index myocardial infarction from 1,038 patients of this trial, using a simple automated latex agglutination (LA) assay in parallel with the standard ELISA test. Results show a somewhat higher mean value for the LA assay (702 ± 1092 vs. 638 ± 986 ng/ml, p = 0.0002), a strong linear correlation of the two assays (r = 0.86) and 88% agreement for values below 500 ng/ml by the ELISA test. D-dimer concentrations determined by each assay were highly correlated in patients with subsequent coronary artery events (p = 0.93) and quartile values for both the LA and ELISA were equally predictive of such events (p = 0.003 and p = 0.001, respectively). This is the first demonstration that a latex agglutination assay for D-dimer can be used to assess the prognostic risk of recurrent coronary thrombotic disease after myocardial infarction

Immunodetection of Ostertagia ostertagi in Cattle Milk Samples in Wasit Province

2017 ◽  
Vol 1 (28) ◽  
pp. 637-648
Author(s):  
Abbas Hassan Khlaty Al-Sray
Keyword(s):  
Milk Production ◽  
Herd Size ◽  
Elisa Test ◽  
Production Factor ◽  
Size Factor ◽  
Ostertagia Ostertagi ◽  
Milk Samples ◽  
Management Group ◽  
Breed Group ◽  
Age Factor

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ³18 liters/day group, 14.29 % for ³10-18 liters/day group, and 25 % for < 10 liters/day group; in age factor, 13.41% for ³3-6 years group, and 14.75% for >6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for <25 (cow/herd) group, and 17.78% for ³ 25 (cow/herd) group. Statistically, the significant differences (P£ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.

MONITORING STUDIES OF ARBOVIRUS INFECTIONS TRANSMITTED BY MOSQUITOES ON THE TERRITORY OF THE VOLGOGRAD REGION

2019 ◽  
pp. 60-66
Author(s):  
E.V. Molchanova ◽  
D.N. Luchinin ◽  
A.O. Negodenko ◽  
D.R. Prilepskaya ◽  
N.V. Boroday ◽  
...  
Keyword(s):  
Virus Antigen ◽  
Elisa Test ◽  
Serum Samples ◽  
Blood Sucking ◽  
Tick Borne Encephalitis ◽  
Volgograd Region ◽  
Two Samples ◽  
Probable Presence ◽  
California Serogroup ◽  
Tick Borne Encephalitis Virus

The paper presents data from the monitoring studies’ results of arbovirus infections transmitted by mosquitoes in the Volgograd region. West Nile virus antigen (WNV) in 9 samples, Tahyna virus in one sample, Batai virus in two samples were detected in the study of 110 samples of field material (blood-sucking mosquitoes) by ELISA test. Antibodies to WNV in 16.58 percent of the samples, to tick-borne encephalitis virus in 1.08 percent, to viruses of the California serogroup and Ukuniemi in 1.09 percent, to the virus Sindbis in 2.17 percent were detected as a result of the study of blood serum samples from donors in the Volgograd region. Thus, we obtained data on the probable presence of the Batai, Sindbis, Ukuniemi and Californian serogroup viruses along with the circulation of WNV on the territory of the Volgograd region.

Sign in / Sign up

Export Citation Format

Share Document