scholarly journals Olfaction across the water–air interface in anuran amphibians

2021 ◽  
Vol 383 (1) ◽  
pp. 301-325
Author(s):  
Lukas Weiss ◽  
Ivan Manzini ◽  
Thomas Hassenklöver
Keyword(s):  
Structural Organization ◽  
Terrestrial Environment ◽  
Animal Group ◽  
Sense Of Smell ◽  
Anuran Amphibians ◽  
Air Interface ◽  
Formidable Challenge ◽  
Olfactory Systems ◽  
And Function ◽  
Derivatives Of

AbstractExtant anuran amphibians originate from an evolutionary intersection eventually leading to fully terrestrial tetrapods. In many ways, they have to deal with exposure to both terrestrial and aquatic environments: (i) phylogenetically, as derivatives of the first tetrapod group that conquered the terrestrial environment in evolution; (ii) ontogenetically, with a development that includes aquatic and terrestrial stages connected via metamorphic remodeling; and (iii) individually, with common changes in habitat during the life cycle. Our knowledge about the structural organization and function of the amphibian olfactory system and its relevance still lags behind findings on mammals. It is a formidable challenge to reveal underlying general principles of circuity-related, cellular, and molecular properties that are beneficial for an optimized sense of smell in water and air. Recent findings in structural organization coupled with behavioral observations could help to understand the importance of the sense of smell in this evolutionarily important animal group. We describe the structure of the peripheral olfactory organ, the olfactory bulb, and higher olfactory centers on a tissue, cellular, and molecular levels. Differences and similarities between the olfactory systems of anurans and other vertebrates are reviewed. Special emphasis lies on adaptations that are connected to the distinct demands of olfaction in water and air environment. These particular adaptations are discussed in light of evolutionary trends, ontogenetic development, and ecological demands.

scholarly journals An update on anatomy and function of the teleost olfactory system

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7808 ◽  
Author(s):  
Jesús Olivares ◽  
Oliver Schmachtenberg
Keyword(s):  
Olfactory System ◽  
Olfactory Receptor Neurons ◽  
Odorant Receptors ◽  
Odor Coding ◽  
Animal Group ◽  
Different Types ◽  
Olfactory Systems ◽  
Receptor Neurons ◽  
Olfactory Organs ◽  
And Function

About half of all extant vertebrates are teleost fishes. Although our knowledge about anatomy and function of their olfactory systems still lags behind that of mammals, recent advances in cellular and molecular biology have provided us with a wealth of novel information about the sense of smell in this important animal group. Its paired olfactory organs contain up to five types of olfactory receptor neurons expressing OR, TAAR, VR1- and VR2-class odorant receptors associated with individual transduction machineries. The different types of receptor neurons are preferentially tuned towards particular classes of odorants, that are associated with specific behaviors, such as feeding, mating or migration. We discuss the connections of the receptor neurons in the olfactory bulb, the differences in bulbar circuitry compared to mammals, and the characteristics of second order projections to telencephalic olfactory areas, considering the everted ontogeny of the teleost telencephalon. The review concludes with a brief overview of current theories about odor coding and the prominent neural oscillations observed in the teleost olfactory system.

scholarly journals Islands of complex DNA are widespread in Drosophila centric heterochromatin.

Genetics ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 283-303
Author(s):  
M H Le ◽  
D Duricka ◽  
G H Karpen
Keyword(s):  
Dna Sequences ◽  
Structure And Function ◽  
Southern Analysis ◽  
Cloning And Sequencing ◽  
Pulsed Field ◽  
Drosophila Heterochromatin ◽  
And Function ◽  
Heterochromatin Structure ◽  
Derivatives Of ◽  
Eukaryotic Genomes

Abstract Heterochromatin is a ubiquitous yet poorly understood component of multicellular eukaryotic genomes. Major gaps exist in our knowledge of the nature and overall organization of DNA sequences present in heterochromatin. We have investigated the molecular structure of the 1 Mb of centric heterochromatin in the Drosophila minichromosome Dp1187. A genetic screen of irradiated minichromosomes yielded rearranged derivatives of Dp1187 whose structures were determined by pulsed-field Southern analysis and PCR. Three Dp1187 deletion derivatives and an inversion had one breakpoint in the euchromatin and one in the heterochromatin, providing direct molecular access to previously inaccessible parts of the heterochromatin. End-probed pulsed-field restriction mapping revealed the presence of at least three "islands" of complex DNA, Tahiti, Moorea, and Bora Bora, constituting approximately one half of the Dp1187 heterochromatin. Pulsed-field Southern analysis demonstrated that Drosophila heterochromatin in general is composed of alternating blocks of complex DNA and simple satellite DNA. Cloning and sequencing of a small part of one island, Tahiti, demonstrated the presence of a retroposon. The implications of these findings to heterochromatin structure and function are discussed.

Effects of avertin versus xylazine-ketamine anesthesia on cardiac function in normal mice

2001 ◽  
Vol 281 (5) ◽  
pp. H1938-H1945 ◽  
Author(s):  
Chari Y. T. Hart ◽  
John C. Burnett ◽  
Margaret M. Redfield
Keyword(s):  
Cardiac Function ◽  
Diastolic Pressure ◽  
Systolic Function ◽  
Pressure Rise ◽  
Left Ventricular ◽  
Lv Function ◽  
Ketamine Anesthesia ◽  
The Difference ◽  
And Function ◽  
Derivatives Of

Anesthetic regimens commonly administered during studies that assess cardiac structure and function in mice are xylazine-ketamine (XK) and avertin (AV). While it is known that XK anesthesia produces more bradycardia in the mouse, the effects of XK and AV on cardiac function have not been compared. We anesthetized normal adult male Swiss Webster mice with XK or AV. Transthoracic echocardiography and closed-chest cardiac catheterization were performed to assess heart rate (HR), left ventricular (LV) dimensions at end diastole and end systole (LVDd and LVDs, respectively), fractional shortening (FS), LV end-diastolic pressure (LVEDP), the time constant of isovolumic relaxation (τ), and the first derivatives of LV pressure rise and fall (dP/d t max and dP/d t min, respectively). During echocardiography, HR was lower in XK than AV mice (250 ± 14 beats/min in XK vs. 453 ± 24 beats/min in AV, P < 0.05). Preload was increased in XK mice (LVDd: 4.1 ± 0.08 mm in XK vs. 3.8 ± 0.09 mm in AV, P < 0.05). FS, a load-dependent index of systolic function, was increased in XK mice (45 ± 1.2% in XK vs. 40 ± 0.8% in AV, P < 0.05). At LV catheterization, the difference in HR with AV (453 ± 24 beats/min) and XK (342 ± 30 beats/min, P < 0.05) anesthesia was more variable, and no significant differences in systolic or diastolic function were seen in the group as a whole. However, in XK mice with HR <300 beats/min, LVEDP was increased (28 ± 5 vs. 6.2 ± 2 mmHg in mice with HR >300 beats/min, P < 0.05), whereas systolic (LV dP/d t max: 4,402 ± 798 vs. 8,250 ± 415 mmHg/s in mice with HR >300 beats/min, P < 0.05) and diastolic (τ: 23 ± 2 vs. 14 ± 1 ms in mice with HR >300 beats/min, P < 0.05) function were impaired. Compared with AV, XK produces profound bradycardia with effects on loading conditions and ventricular function. The disparate findings at echocardiography and LV catheterization underscore the importance of comprehensive assessment of LV function in the mouse.

scholarly journals Molecular mechanisms of olfactory detection in insects: beyond receptors

Open Biology ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 200252
Author(s):  
Hayden R. Schmidt ◽  
Richard Benton
Keyword(s):  
Molecular Mechanisms ◽  
Olfactory Receptors ◽  
Environmental Chemicals ◽  
Ecological Niches ◽  
Cellular Mechanisms ◽  
Signalling Molecules ◽  
Olfactory Systems ◽  
Lymph Fluid ◽  
And Function

Insects thrive in diverse ecological niches in large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus in the study of insect olfaction has been on the role of olfactory receptors in mediating neuronal responses to environmental chemicals. In vivo , these receptors operate in specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid and a precisely shaped cuticle. While sensilla are inherent to odour sensing in insects, we are only just beginning to understand their construction and function. Here, we review recent work that illuminates how odour-evoked neuronal activity is impacted by sensillar morphology, lymph fluid biochemistry, accessory signalling molecules in neurons and the physiological crosstalk between sensillar cells. These advances reveal multi-layered molecular and cellular mechanisms that determine the selectivity, sensitivity and dynamic modulation of odour-evoked responses in insects.

scholarly journals Students’ ability to correctly apply differentiation rules to structurally different functions

2018 ◽  
Vol 114 (11/12) ◽  
Author(s):  
Aneshkumar Maharaj ◽  
Mthobisi Ntuli
Keyword(s):  
Chain Rule ◽  
First Year ◽  
Symbolic Form ◽  
First Year Students ◽  
University Mathematics ◽  
Online Quizzes ◽  
And Function ◽  
Derivatives Of ◽  
Derivative Concept ◽  
Function Structures

The derivative concept is studied in first-year university mathematics. In this study, we focused on students’ ability to correctly apply the rules for derivatives of functions with the different structures that they encounter in their university studies. This was done by investigating the online responses of first-year students at the University of KwaZulu-Natal to online quizzes that contributed to their assessment. Based on this investigation, we then interviewed eight students to gain an insight into the thinking behind their responses. We report on the analysis of students’ responses to five items on the online quizzes based on the derivative concept. The categories in which those items were based are: condition for existence of derivative at a point; rules for derivatives of standard functions; application of chain rule to different function structures; the application of multiple rules; and application of derivatives to optimise a function. Our findings indicate that students had difficulty in detecting that multiple rules for derivatives were required to differentiate certain types of functions represented in symbolic form. Furthermore, students had difficulty in finding the derivative of a function when more than one application of the chain rule was required. However, there were students who had the ability to apply the rules for derivatives of functions without difficulty. In particular, most of the students were able to correctly recall the differentiation rules for functions with standard structures f(x)=xn, h(x)=ekx and y=[g(x)]n, n 0 and k is a non-zero constant. Students were also able to correctly apply the chain rule to an exponential function with base e, raised to 4x. The majority of students were able to correctly apply the chain rule together with differentiation rules for logarithmic and exponential (with bases a >1) function structures, and function structures that required the application of the product rule together with the chain rule. Most of the students were able to apply derivatives to optimise a function. Significance: A significant percentage of students who took online quizzes experienced difficulties with applying multiple differentiation rules in the context of a single function. The difficulties stemmed from their inability to detect from the structure of the function which rules should be applied and also the order in which those relevant rules should be applied.

scholarly journals Structural Organization of Procaryotic and Eucaryotic Hsp90. INFLUENCE OF DIVALENT CATIONS ON STRUCTURE AND FUNCTION

1995 ◽  
Vol 270 (24) ◽  
pp. 14412-14419 ◽  
Author(s):  
Ursula Jakob ◽  
Ines Meyer ◽  
Hans Bügl ◽  
Stefanie André ◽  
James C. A. Bardwell ◽  
...  
Keyword(s):  
Structural Organization ◽  
Divalent Cations ◽  
Structure And Function ◽  
And Function

Restoration by salicylate derivatives of localization and function of pendrin mutants

2016 ◽  
Vol 2016.27 (0) ◽  
pp. B104
Author(s):  
Taiki CHAEN ◽  
Michio MURAKOSHI ◽  
Kenichiro MATSUZAKI ◽  
Hiroshi WADA
Keyword(s):  
And Function ◽  
Derivatives Of

MAP1 structural organization in Drosophila: in vivo analysis of FUTSCH reveals heavy- and light-chain subunits generated by proteolytic processing at a conserved cleavage site

2008 ◽  
Vol 414 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Beiyan Zou ◽  
Huaru Yan ◽  
Fumiko Kawasaki ◽  
Richard W. Ordway
Keyword(s):  
Light Chain ◽  
Neural Development ◽  
Cleavage Site ◽  
Structural Organization ◽  
Single Gene ◽  
Proteolytic Cleavage ◽  
Proteolytic Processing ◽  
In Vivo Analysis ◽  
And Function

The MAP1 (microtubule-associated protein 1) family is a class of microtubule-binding proteins represented by mammalian MAP1A, MAP1B and the recently identified MAP1S. MAP1A and MAP1B are expressed in the nervous system and thought to mediate interactions of the microtubule-based cytoskeleton in neural development and function. The characteristic structural organization of mammalian MAP1s, which are composed of heavy- and light-chain subunits, requires proteolytic cleavage of a precursor polypeptide encoded by the corresponding map1 gene. MAP1 function in Drosophila appears to be fulfilled by a single gene, futsch. Although the futsch gene product is known to share several important functional properties with mammalian MAP1s, whether it adopts the same basic structural organization has not been addressed. Here, we report the identification of a Drosophila MAP1 light chain, LCf, produced by proteolytic cleavage of a futsch-encoded precursor polypeptide, and confirm co-localization and co-assembly of the heavy chain and LCf cleavage products. Furthermore, the in vivo properties of MAP1 proteins were further defined through precise MS identification of a conserved proteolytic cleavage site within the futsch-encoded MAP1 precursor and demonstration of light-chain diversity represented by multiple LCf variants. Taken together, these findings establish conservation of proteolytic processing and structural organization among mammalian and Drosophila MAP1 proteins and are expected to enhance genetic analysis of conserved MAP1 functions within the neuronal cytoskeleton.

scholarly journals Parallel Olfactory Systems in Insects: Anatomy and Function

2010 ◽  
Vol 55 (1) ◽  
pp. 399-420 ◽  
Author(s):  
C. Giovanni Galizia ◽  
Wolfgang Rössler
Keyword(s):  
Olfactory Systems ◽  
And Function
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