Efficient biosynthesis of polysaccharide welan gum in heat shock protein-overproducing Sphingomonas sp. via temperature-dependent strategy

Seed germination is an energy demanding process that requires functional mitochondria upon imbibition. However, how mitochondria fine tune seed germination, especially in response to the dynamics of environmental temperature, remains largely unknown at the molecular level. Here, we report a mitochondrial matrix-localized heat shock protein GhHSP24.7, that regulates seed germination in a temperature-dependent manner. Suppression ofGhHSP24.7renders the seed insensitive to temperature changes and delays germination. We show that GhHSP24.7 competes with GhCCMH to bind to the maturation subunit protein GhCcmFcto form cytochrome C/C1(CytC/C1) in the mitochondrial electron transport chain. GhHSP24.7 modulates CytC/C1production to induce reactive oxygen species (ROS) generation, which consequently accelerates endosperm rupture and promotes seed germination. Overexpression ofGhHSP24.7’s homologous genes can accelerate seed germination inArabidopsisand tomato, indicating its conserved function across plant species. Therefore,HSP24.7is a critical factor that positively controls seed germination via temperature-dependent ROS generation.

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Temperature-dependent subunit exchange and chaperone-like activities of Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2004.02.053 ◽

2004 ◽

Vol 316 (2) ◽

pp. 291-299 ◽

Cited By ~ 38

Author(s):

Xinmiao Fu ◽

Zengyi Chang

Keyword(s):

Mycobacterium Tuberculosis ◽

Heat Shock ◽

Heat Shock Protein ◽

Small Heat Shock Protein ◽

Temperature Dependent ◽

Subunit Exchange

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The Temperature-Dependent Selectivity of Potential Interaction Partners for the Small Heat Shock Protein IbpA from Acholeplasma laidlawii

BioNanoScience ◽

10.1007/s12668-016-0259-0 ◽

2016 ◽

Vol 6 (4) ◽

pp. 437-442 ◽

Cited By ~ 3

Author(s):

Innokentii E. Vishnyakov ◽

Mikhail I. Bogachev ◽

Ilnur Salafutdinov ◽

Sergei N. Borchsenius ◽

Airat R. Kayumov

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Small Heat Shock Protein ◽

Potential Interaction ◽

Temperature Dependent ◽

Acholeplasma Laidlawii ◽

Interaction Partners

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Analysis of the temperature-dependent temporal pattern of heat-shock-protein synthesis in fish cells

Bioscience Reports ◽

10.1007/bf01172875 ◽

1983 ◽

Vol 3 (7) ◽

pp. 647-658 ◽

Cited By ~ 23

Author(s):

Lashitew Gedamu ◽

Beverly Culham ◽

John J. Heikkila

Keyword(s):

Protein Synthesis ◽

Heat Shock ◽

Heat Shock Protein ◽

Temporal Pattern ◽

Stress Proteins ◽

Incubation Temperature ◽

Stress Protein ◽

In Vitro Translation ◽

Continuous Exposure ◽

Temperature Dependent

Continuous exposure of Chinook salmon embryo cells to an elevated incubation temperature of 24°C induces the transient expression of a set of heat-shock or stress proteins whereas maintenance of the cells at a higher incubation temperature of 28°C produces a continuous synthesis of these stress proteins. In vitro translation studies suggest that the temperature-dependent temporal pattern of stress-protein synthesis is correlated with the levels of stress-protein mRNA. This was verified using a recombinant-DNA probe complementary to the 70K heat-shock-protein mRNA. A transient increase in the level of the fish heat-shock 70K mRNA was observed in RNA samples isolated from cells continuously exposed at 24°C However, a constant increase in the level of this specific mRNA was found in RNA preparations obtained from cells maintained at 28°C Therefore, the temperature-dependent pattern of fish heat-shockprotein synthesis appears to be directly related to the level of heat-shock-protein mRNA.

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A Temperature-Dependent Switch from Chaperone to Protease in a Widely Conserved Heat Shock Protein

Cell ◽

10.1016/s0092-8674(00)80743-6 ◽

1999 ◽

Vol 97 (3) ◽

pp. 339-347 ◽

Cited By ~ 517

Author(s):

Christoph Spiess ◽

Alexandra Beil ◽

Michael Ehrmann

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Temperature Dependent

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Temperature-dependent pattern of heat shock protein synthesis in psychrophilic and psychrotrophic microorganisms

Canadian Journal of Microbiology ◽

10.1139/m86-094 ◽

1986 ◽

Vol 32 (6) ◽

pp. 516-521 ◽

Cited By ~ 13

Author(s):

Kirk L. McCallum ◽

John J. Heikkila ◽

William E. Inniss

Keyword(s):

Protein Synthesis ◽

Heat Shock ◽

Heat Shock Proteins ◽

Heat Shock Protein ◽

Incubation Temperature ◽

The Arctic ◽

Transcriptional Level ◽

Psychrophilic Bacterium ◽

Temperature Dependent ◽

Shock Proteins

The patterns of proteins synthesized by the arctic psychrophilic bacterium Res-10 and the psychrotroph Bacillus psychrophilus during various heat shocks up to 32 °C were examined. Both microorganisms were found to display temperature-dependent patterns of heat shock protein synthesis. Elevation of the incubation temperature of the arctic psychrophile from 0 to 15, 20, 25, or 32 °C induced the synthesis of at least 19 heat shock proteins. Imposing similar heat shock upon cells of the psychrotroph resulted in the induction of at least 25 heat shock proteins. Examination of the effect of the transcriptional inhibitor rifampicin on the synthesis of heat shock proteins revealed that the primary control of heat shock protein synthesis lies at the transcriptional level in both microorganisms.

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