Molecular characteristics of extended-spectrum beta-lactamases among gram-negative isolates collected in Cairo University Hospital

OBJECTIVES: To compare the activity of piperacillin-tazobactam with piperacillin and other parenterally administered antibiotics against aerobic Gram-negative bacilli and Gram-positive cocci isolated from across Canada, and to determine the prevalence of resistance mediated by extended-spectrum cephalosporinases.METHODS: Sixty-one laboratories participated. Disk diffusion testing was performed in accordance with methods outlined by the National Committee for Clinical Laboratory Standards. Susceptibilities were performed on 8206 strains.Escherichia coliandKlebsiella pneumoniaewith reduced susceptibilities to third-generation cephalosporins were screened for extended-spectrum beta-lactamases (ESBLs).RESULTS: Piperacillin-tazobactam was active against 92% of the strains, piperacillin against 81% and ticarcillin-clavulanic acid against 88%. Few differences were observed in the relative susceptibility of strains from teaching or community hospitals, from different anatomic sites or from different regions of the country. Aerobic Gram-negative bacilli tested tended to be more susceptible to all the agents than was recently reported in a similar American study. Only 43% ofEnterococcus faeciumwere susceptible to ampicillin and 42% to piperacillin piperacillin with and without tazobactam. Only two enterococcal strains were resistant to vancomycin, and 19 had intermediate zone sizes. Of the 10 strains ofE coliand eight strains ofK pneumoniaewith reduced susceptibility to extended spectrum cephalosporins, only one demonstrated typical ESBL activity.CONCLUSIONS: Canadian aerobic Gram-positive cocci and Gram-negative bacilli remain highly susceptible to many currently available antibiotics. The findings confirm a broad spectrum of activity of piperacillin-tazobactam and indicate that the pattern of susceptibility is quite uniform from different body sites, in both teaching and community hospitals, and across the country.

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Cedecea lapagei An Extremely Rare Uropathogen: A Case Report and Review of the Literature

10.21203/rs.3.rs-181472/v1 ◽

2021 ◽

Author(s):

Abdikarim Hussein Mohamed ◽

Hussein Ali Mohamud

Keyword(s):

Renal Failure ◽

Case Report ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Beta Lactam ◽

Gram Negative ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Tract Infections

Abstract Background: Gram-negative enterobacteria are the most common cause of urinary tract infections. Cedecea is a new separate genus in the family enterobacteriaceae, and it is a very rare pathogen that was primarily found in the respiratory tract. Cedecea lapagei is a very rare pathogen of urinary tract infections. To the best of our knowledge, this is the first case report in the world reported in English literature. Case presentation: A 55 years old man with chronic renal failure, poorly controlled diabetes mellitus, and hypertension presented with acute exacerbations of renal failure and irritative voiding symptoms. After stabilization and empirical antibiotic therapy with Ceftriaxone, the patient’s condition was not improved and deteriorated progressively. After the request of urine culture, the culture was isolated, an extremely rare uropathogen recently recognized by the Centers for Disease Control and Prevention (CDC); the Cedecea lapagei. Cedecea lapagei identification had been done using Eosin methylene blue agar (EMB). Gram-negative lipase positive bacteria with bacillus in shape, motile in nature that is non-spore-forming, and non-encapsulated enterobacteria with the final result of >100,000 colony-forming units per ml of Cedecea lapagei were isolated. Mueller-Hinton agar had been used to perform antimicrobial sensitivity and resistance. The pathogen was extensively resistant to the extended-spectrum beta-lactamases antibiotics and extended-spectrum beta-lactam inhibitors while carbapenems, fluoroquinolones, aminoglycosides, and Trimethoprim-sulfamethoxazole showed a higher sensitivity rate. Conclusion: The treatment of Cedecea lapagei infections represents a challenging issue due to its multi-drug resistant and extensive drug resistance patterns to a variety of antimicrobial classes, such as extended-spectrum beta-lactamases, cephalosporins, and beta-lactam inhibitors. Antimicrobial treatment should be aligned with the culture findings once available.

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Prevalence and Genotypic Characterization of Extended- Spectrum Beta-Lactamases Produced by Gram Negative Bacilli at a Tertiary Care Hospital in Rural South Western Uganda

British Microbiology Research Journal ◽

10.9734/bmrj/2014/9792 ◽

2014 ◽

Vol 4 (12) ◽

pp. 1541-1550 ◽

Cited By ~ 2

Author(s):

Acaku Moses ◽

Freddie Bwanga ◽

Yap Boum ◽

Joel Bazira

Keyword(s):

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Rural South ◽

Gram Negative ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Western Uganda

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Faculty Opinions recommendation of Pediatric multicenter evaluation of the Verigene gram-negative blood culture test for rapid detection of inpatient bacteremia involving gram-negative organisms, extended-spectrum beta-lactamases, and carbapenemases.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718361676.793498602 ◽

2014 ◽

Author(s):

Stan Deresinski

Keyword(s):

Blood Culture ◽

Rapid Detection ◽

Negative Blood Culture ◽

Gram Negative ◽

Beta Lactamases ◽

Culture Test ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases ◽

Gram Negative Organisms

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Prevalence of CTX M Extended-Spectrum Beta-Lactamases in Clinical Gram-Negative Bacteria

Bahrain Medical Bulletin ◽

10.12816/0008130 ◽

2014 ◽

Vol 36 (4) ◽

pp. 228-231 ◽

Cited By ~ 1

Author(s):

Mohammad Shahid

Keyword(s):

Gram Negative Bacteria ◽

Gram Negative ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases

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Antibiotic Susceptibility Pattern of Gram-negative Isolates of Lower Respiratory Tract Infection

Journal of Nepal Health Research Council ◽

10.3126/jnhrc.v16i1.19358 ◽

2018 ◽

Vol 16 (1) ◽

pp. 22-26 ◽

Cited By ~ 1

Author(s):

Rakshya Nepal ◽

Basudha Shrestha ◽

Deepak Man Joshi ◽

Rajesh Dhoj Joshi ◽

Sanjit Shrestha ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Lower Respiratory Tract Infection ◽

Lower Respiratory Tract ◽

Acinetobacter Calcoaceticus ◽

Gram Negative ◽

Beta Lactamases ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases

Background: Lower respiratory tract infection is a common cause of morbidity and mortality worldwide. A crosssectional study was carried out with an objective to study the antibiogram of Gram-negative isolates of patients with lower respiratory tract infection visiting Kathmandu Model Hospital.Methods: A total of 274 specimens including sputum, endotracheal aspirates, suction tips were cultured as per standard microbiological technique. Antibiotic susceptibility and detection of Extended-spectrum beta- lactamases (ESBLs) were performed following Clinical Laboratory Standard Institute (CLSI 2014) guidelines.Results: Respiratory pathogens were recovered from 24.6% (n=65) cases. Klebsiella pneumoniae (40%) was the commonest isolates. The highest prevalence of multidrug-resistance (69.23%) was observed in Acinetobacter calcoaceticus baumannii complex. Extended-spectrum beta- lactamases were detected in Escherichia coli (n=4), Klebsiella pneumoniae (n=4) and Acinetobacter calcoaceticus baumannii complex (n=1).Conclusions: High prevalence of multidrug-resistance and extended- spectrum beta- lactamase producers were observed in respiratory isolates. For effective management of lower respiratory tract infections, an ultimate and detailed microbiological diagnosis and susceptibility testing is required.

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