Effects of vitamin C on epididymal sperm quality following experimentally induced copper poisoning in mice

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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A Simple and Practical Method for Rat Epididymal Sperm Count (Rattus norvegicus)

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2015.41.1-3 ◽

2015 ◽

Vol 4 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Muhammad Ja’far Luthfi

Keyword(s):

Rattus Norvegicus ◽

Sperm Quality ◽

Sperm Count ◽

Practical Method ◽

Quality Analysis ◽

Minimal Amount ◽

Sperm Number ◽

Epididymal Sperm ◽

Sperm Sample

<p>Sperm sample from epididymal source can be determined its number using minimal amount of equipment. These method will aid researcher and practitioner in sperm quality analysis to determined sperm number rapidly and practically.</p>

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Effect of vitamin C on the growth of experimentally induced endometriotic cysts

Journal of Obstetrics and Gynaecology Research ◽

10.1111/jog.12050 ◽

2013 ◽

Vol 39 (7) ◽

pp. 1253-1258 ◽

Cited By ~ 14

Author(s):

Yildirim Durak ◽

Arif Kokcu ◽

Mehmet Kefeli ◽

Devran Bildircin ◽

Handan Çelik ◽

...

Keyword(s):

Vitamin C ◽

Experimentally Induced

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Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Zygote ◽

10.1017/s0967199418000230 ◽

2018 ◽

Vol 26 (4) ◽

pp. 301-307 ◽

Cited By ~ 2

Author(s):

José A. B. Bezerra ◽

Andréia M. Silva ◽

Patrícia C Sousa ◽

Lívia B. Campos ◽

Érica C. G. Praxedes ◽

...

Keyword(s):

Sperm Quality ◽

Semen Analysis ◽

Membrane Integrity ◽

Egg Yolk ◽

Coconut Water ◽

Computer Assisted ◽

Epididymal Sperm ◽

Sperm Plasma Membrane ◽

Pecari Tajacu ◽

Functional Membrane

SummaryThe aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

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Comparison of three different media for freezing of epididymal sperm from the African buffalo (Syncerus caffer) and influence of equilibration time on the post-thaw sperm quality

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v71i3.261 ◽

2004 ◽

Vol 71 (3) ◽

Cited By ~ 4

Author(s):

F.C. Herold ◽

K. De Haas ◽

D. Cooper ◽

B. Colenbrander ◽

J.O. Nothling ◽

...

Keyword(s):

South Africa ◽

Sperm Quality ◽

The Other ◽

Semen Parameters ◽

Equilibration Time ◽

African Buffalo ◽

Epididymal Sperm ◽

Progressive Motility ◽

Reproductive Techniques ◽

Buffalo Semen

Assisted reproductive techniques might prove themselves useful tools in producing buffaloes free of specific diseases (BFSD), which are in demand in South Africa. Freezing protocols for African buffalo semen must not only result in good post-thaw qualities, but must also be practical. Epididymal sperm from six mature African buffalo bulls was collected, diluted with three different semen extenders and frozen. Pre-freezing equilibration times between 2 and 9 h were tested. Total and progressive motility, longevity and acrosomal integrity were measured and compared. The use of TriladylTM proved to result in better post-thaw parameters than the other two diluents. Equilibration times of between 4 and 9 h did not influence post-thaw sperm qualities significantly. For some of the treatments, exposure to semen extenders before freezing for less than 4 h resulted in inferior post-thaw semen parameters.

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Sperm viability and morphology of two genetically diverse Merino lines

Reproduction Fertility and Development ◽

10.1071/rd99041 ◽

2000 ◽

Vol 12 (6) ◽

pp. 337 ◽

Cited By ~ 2

Author(s):

H. Lambrechts ◽

F. E. van Niekerk ◽

S. W. P. Cloete ◽

W. A. Coetzer ◽

G. van der Horst

Keyword(s):

Adverse Effect ◽

Sperm Motility ◽

Sperm Quality ◽

Sperm Parameters ◽

Sperm Viability ◽

Base Population ◽

Epididymal Sperm ◽

High Line ◽

Computer Aided

Microscopically evaluated sperm parameters, as well as computer-aided sperm motility analysis (CASMA), were used to assess sperm quality and the effect of cryopreservation on ram semen obtained from two genetically diverse Merino lines. These lines were divergently selected on maternal ranking values for multiple rearing ability from the same base population since 1986. Replacements in the high (+) line were preferentially the progeny of ewes rearing >1 lamb per joining. Progeny of ewes rearing <1 lamb per joining was preferred as replacements in the low (–) line. Sperm quality, as assessed by percentages of live, abnormal and acrosome-intact spermatozoa as well as by motility, was independent (P≤0.20) of line, time of sampling and their interaction in ejaculated samples obtained from the eight rams used as sires in 1995. Sperm quality of frozen–thawed samples was adversely affected (P≤0.01) by cryopreservation and thawing at 35˚C for 30 s relative to fresh ejaculated samples. No consistent differences between lines were found in epididymal sperm samples obtained from 12 slaughtered rams (6 from each line). The adverse effect (P≤0.05) of cryopreservation and thawing at 35˚C for 30 s on sperm viability and motility was also demonstrated for these samples.

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