Comparative evaluation of the effects of batroxobin/calcium gluconate-activated platelet-rich plasma and calcium chloride-activated platelet-rich plasma on tibial fractures of Nigerian local dogs

Platelet-rich plasma (PRP) therapy has been considered as a promising treatment for androgenetic alopecia (AGA). The aim of the study was comparative evaluation of the clinical efficacy of PRP-therapy, minoxidil, and their combination in the treatment of men with AGA and to evaluate the effects of PRP on the proliferation of hair follicle (HF) cells in skin biopsy. Materials and Methods: The study involved 69 men who were divided into 3 groups who received PRP therapy, minoxidil, and their combination. The clinical efficacy of the therapy was evaluated by the dynamics of morphometric of hairs. To assess cell proliferation antibodies to β-catenin, CD34, Ki67, and to Dkk-1 were used. Results. PRP treatment was more effective than minoxidil therapy (p = 0.005). Complex therapy turned out to be more effective than minoxidil monotherapy (p < 0.0001) and PRP monotherapy (p = 0.007). After applying PRP the absolute and relative values of the β-catenin and CD34 expression area increased; an increase in Ki67+ index was also significant. Conclusions: PRP can be considered as a treatment option for AGA. Combined PRP and minoxidil use seems promising for the treatment of AGA. PRP increase in the proliferative activity of HF cells and improves hair morphology in patients with AGA.

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Assessment of Vascular Endothelial Growth Factor in Fresh versus Frozen Platelet Rich Plasma

Journal of Blood Transfusion ◽

10.1155/2015/706903 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Nada Hosny ◽

Fikry Goubran ◽

Basma BadrEldin Hasan ◽

Noha Kamel

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Calcium Gluconate ◽

Platelet Rich Plasma ◽

Vascular Endothelial ◽

High Concentration ◽

Significant Difference ◽

Growth Factor Release ◽

Endothelial Growth Factor ◽

Vegf Release

Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05). No significant difference was found between VEGF concentrations after activating fresh and frozen-thawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.

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Compatibility of Calcium Chloride and Calcium Gluconate with Sodium Phosphate in a Mixed TPN Solution

American Journal of Health-System Pharmacy ◽

10.1093/ajhp/37.5.673 ◽

1980 ◽

Vol 37 (5) ◽

pp. 673-674 ◽

Cited By ~ 3

Author(s):

R. Scott Henry ◽

Raymond W. Jurgens ◽

Roy Sturgeon ◽

Narayan Athanikar ◽

Alan Welco ◽

...

Keyword(s):

Calcium Chloride ◽

Calcium Gluconate ◽

Sodium Phosphate

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Comparison and Investigation of Exosomes Derived from Platelet-Rich Plasma Activated by Different Agonists

Cell Transplantation ◽

10.1177/09636897211017833 ◽

2021 ◽

Vol 30 ◽

pp. 096368972110178

Author(s):

Shunli Rui ◽

Yi Yuan ◽

Chenzhen Du ◽

Peiyang Song ◽

Yan Chen ◽

...

Keyword(s):

Calcium Gluconate ◽

Platelet Rich Plasma ◽

Saline Group ◽

Signal Pathway ◽

Biological Functions ◽

High Quality ◽

Bioactive Substances ◽

The Difference ◽

Erk Signal Pathway

PRP-Exos are nanoscale cup-shaped vesicles that carry a variety of proteins, mRNAs, microRNAs, and other bioactive substances. PRP-Exos can be formed through several induction pathways, which determine their molecular profiles and facilitate their tailormade participation in intercellular communication. Currently, little is known on how the PRP-Exos activation method influences the quality and quantity of PRP-Exos. The present study aims to observe and analyze the number, profile, and growth factors of PRP-Exos through TEM, Nanoflow, and WB after PRP activation and compare the difference in function of PRP-Exos on HUVECs, with different stimuli (calcium gluconate, thrombin, or both). We found that PRP activated with both thrombin and calcium gluconate harvested the highest concentration of exosomes [(7.16 ± 0.46) × 1010 particles/ml], compared to thrombin group [(4.87 ± 0.15) × 1010 particles/ml], calcium gluconate group [(5.85 ± 0.43) × 1010 particles/ml], or saline group [(7.52 ± 0.19) × 109 particles/ml], respectively ( P < 0.05) via Nanoflow analysis. The WB analysis showed that cytokines (VEGF, PDGFBB, bFGF, TGF-β) are differentially encapsulated in PRP-Exos, depending on the PRP stimulus, in which the mixture-PRP-Exos yielded the highest concentration of cytokines. In the function assay of PRP-Exos on HUVECs, the mixture-PRP-Exos promoted HUVECs proliferation, increased HUVECs migration, promoted the formation of vessel-like by HUVECs via the AKT ERK signal pathway more dramatically, compared with other groups. In summary, our studies showed that PRP activated by the mixture of calcium gluconate and thrombin harvested the best quality of exosomes which had the top biological functions. This study provides a protocol for selecting appropriate PRP activators to obtain high-quality exosomes for future applications.

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Safety of Compounded Calcium Chloride Admixtures for Peripheral Intravenous Administration in the Setting of a Calcium Gluconate Shortage

Journal of Pharmacy Practice ◽

10.1177/0897190013513617 ◽

2013 ◽

Vol 27 (5) ◽

pp. 474-477 ◽

Cited By ~ 3

Author(s):

Kevin E. Anger ◽

Caryn Belisle ◽

Megan B. Colwell ◽

Robert Dannemiller ◽

Burhan Alawadhi ◽

...

Keyword(s):

Adverse Drug Reaction ◽

Calcium Chloride ◽

Calcium Gluconate ◽

Grading System ◽

Inpatient Setting ◽

Site Reaction ◽

Infusion Site ◽

System A ◽

Time Period ◽

Low Incidence

Calcium gluconate is preferred over calcium chloride for intravenous (IV) repletion of calcium deficiencies in the inpatient setting. In the setting of a national shortage of IV calcium gluconate, our institution implemented a compounded calcium chloride admixture for IV administration. The objective of this analysis is to evaluate the peripheral infusion site safety of compounded IV calcium chloride admixtures in adult inpatients. A total of 222 patients, encompassing 224 inpatient admissions, from April to June 2011 were retrospectively reviewed. Sterile preparations of calcium chloride in 5% dextrose (600 mg/250 mL and 300 mg/100 mL) were used during the study time period. Adverse infusion site reactions were assessed using an institutional infiltration and phlebitis grading system. A total of 333 doses were administered peripherally. In all, 4 (1.8%) patients experienced a moderate to severe infusion site reaction, with 3 due to phlebitis and 1 due to infiltration. Naranjo Nomogram for Adverse Drug Reaction Assessment classified all 4 reactions to have a possible link to calcium chloride administration. Peripheral administration of compounded calcium chloride admixtures in 5% dextrose is associated with a low incidence of IV infusion site reactions and can be considered as an alternative in the event of a calcium gluconate shortage.

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