A novel double-stranded RNA virus detected in Primula malacoides is a plant-isolated partitivirus closely related to partitivirus infecting fungal species

2009 ◽  
Vol 154 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Liqiang Li ◽  
Qinghua Tian ◽  
Zhiyou Du ◽  
Greg J. Duns ◽  
Jishuang Chen
Keyword(s):  
Rna Virus ◽  
Fungal Species ◽  
Double Stranded Rna

scholarly journals A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae Is Able to Cross the Species Barrier and Is Deleterious to a New Host

2021 ◽  
Vol 7 (10) ◽  
pp. 861
Author(s):  
Carolina Cornejo ◽  
Sakae Hisano ◽  
Helena Bragança ◽  
Nobuhiro Suzuki ◽  
Daniel Rigling
Keyword(s):  
De Novo ◽  
Rna Virus ◽  
Cryphonectria Parasitica ◽  
Fungal Species ◽  
Species Barrier ◽  
New Host ◽  
Double Stranded Rna ◽  
The Family ◽  
Rapid Amplification ◽  
Segmented Genome

Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Cocultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae upon virus infection. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

scholarly journals A New Double-Stranded RNA Mycovirus in Cryphonectria naterciae is Able to Cross the Species Barrier and is Deleterious to a New Host

2021 ◽  
Author(s):  
Carolina Cornejo ◽  
Sakae Hisano ◽  
Helena Bragança ◽  
Nobuhiro Suzuki ◽  
Daniel Rigling
Keyword(s):  
De Novo ◽  
Rna Virus ◽  
Cryphonectria Parasitica ◽  
Fungal Species ◽  
Species Barrier ◽  
New Host ◽  
Double Stranded Rna ◽  
The Family ◽  
Rapid Amplification ◽  
Segmented Genome

Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized a novel double-stranded RNA virus from the fungal species Cryphonectria naterciae, a species unexplored as a virus host. De novo assembly of RNA-seq data and Sanger sequencing of RACE (rapid amplification of cDNA ends) clones gave the complete, non-segmented genome (10,164 bp) of the virus termed Cryphonectria naterciae fusagravirus (CnFGV1) that was phylogenetically placed within the previously proposed viral family Fusagraviridae. Of 31 field-collected strains of C. naterciae, 40% tested CnFGV1-positive. Co-cultivation resulted in within-species transmission of CnFGV1 to virus-free strains of C. naterciae. Comparison of the mycelium phenotype and the growth rate of CnFGV1-infected and virus-free isogenic strains revealed frequent sectoring and growth reduction in C. naterciae. Co-culturing also led to cross-species transmission of CnFGV1 to Cryphonectria carpinicola and Cryphonectria radicalis, but not to Cryphonectria parasitica. The virus-infected C. naterciae and the experimentally infected Cryphonectria spp. readily transmitted CnFGV1 through asexual spores to the next generation. CnFGV1 strongly reduced conidiation and in some cases vegetative growth of C. carpinicola, which is involved in the European hornbeam disease. This study is the first report of a fusagravirus in the family Cryphonectriaceae and lays the groundwork for assessing a hypovirulence effect of CnFGV1 against the hornbeam decline in Europe.

scholarly journals N6-methyladenosine RNA modification suppresses antiviral innate sensing pathways via reshaping double-stranded RNA

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weinan Qiu ◽  
Qingyang Zhang ◽  
Rui Zhang ◽  
Yangxu Lu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Rna Virus ◽  
Negative Regulator ◽  
Rna Modification ◽  
Type I ◽  
Viral Immunity ◽  
Potential Therapeutic Target ◽  
Viral Dsrna ◽  
Double Stranded Rna ◽  
Genetic Ablation ◽  
Vesicular Stomatitis

AbstractDouble-stranded RNA (dsRNA) is a virus-encoded signature capable of triggering intracellular Rig-like receptors (RLR) to activate antiviral signaling, but whether intercellular dsRNA structural reshaping mediated by the N6-methyladenosine (m6A) modification modulates this process remains largely unknown. Here, we show that, in response to infection by the RNA virus Vesicular Stomatitis Virus (VSV), the m6A methyltransferase METTL3 translocates into the cytoplasm to increase m6A modification on virus-derived transcripts and decrease viral dsRNA formation, thereby reducing virus-sensing efficacy by RLRs such as RIG-I and MDA5 and dampening antiviral immune signaling. Meanwhile, the genetic ablation of METTL3 in monocyte or hepatocyte causes enhanced type I IFN expression and accelerates VSV clearance. Our findings thus implicate METTL3-mediated m6A RNA modification on viral RNAs as a negative regulator for innate sensing pathways of dsRNA, and also hint METTL3 as a potential therapeutic target for the modulation of anti-viral immunity.

scholarly journals Cardiomyopathy Syndrome of Atlantic Salmon (Salmo salar L.) Is Caused by a Double-Stranded RNA Virus of the Totiviridae Family

2011 ◽  
Vol 85 (11) ◽  
pp. 5275-5286 ◽  
Author(s):  
O. Haugland ◽  
A. B. Mikalsen ◽  
P. Nilsen ◽  
K. Lindmo ◽  
B. J. Thu ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Rna Virus ◽  
Double Stranded Rna ◽  
Atlantic Salmon Salmo Salar

The coat protein of the yeast double-stranded RNA virus L-A attaches covalently to the cap structure of eukaryotic mRNA

1992 ◽  
Vol 12 (8) ◽  
pp. 3390-3398
Author(s):  
A Blanc ◽  
C Goyer ◽  
N Sonenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Coat Protein ◽  
Translation Initiation ◽  
Rna Virus ◽  
Binding Protein ◽  
Covalent Bond ◽  
Cellular Proteins ◽  
Double Stranded Rna ◽  
Virus Coat ◽  
Drosophila Cells

The eukaryotic mRNA 5' cap structure m7GpppX (where X is any nucleotide) interacts with a number of cellular proteins. Several of these proteins were studied in mammalian, yeast, and drosophila cells and found to be involved in translation initiation. Here we describe a novel cap-binding protein, the coat protein of L-A, a double-stranded RNA virus that is persistently maintained in many Saccharomyces cerevisiae strains. The results also suggest that the coat protein of a related double-stranded RNA virus (L-BC) is likewise a cap-binding protein. Strikingly, in contrast to the cellular cap-binding proteins, the interaction between the L-A virus coat protein and the cap structure is through a covalent bond.

First Evidence of a Trisegmented Double-Stranded RNA Virus in Canine Faeces

2001 ◽  
Vol 161 (2) ◽  
pp. 205-207 ◽  
Author(s):  
E.M VOLOTÄO ◽  
C.C SOARES ◽  
M.C.M ALBUQUERQUE ◽  
F.M.D.A SILVA ◽  
T.R.B CARVALHO ◽  
...  
Keyword(s):  
Rna Virus ◽  
Double Stranded Rna

scholarly journals Evidence of Intragenic Recombination in African Horse Sickness Virus

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 654 ◽  
Author(s):  
Harry G. Ngoveni ◽  
Antoinette van Schalkwyk ◽  
J.J. Otto Koekemoer
Keyword(s):  
Bluetongue Virus ◽  
Type Species ◽  
Genetic Recombination ◽  
Rna Virus ◽  
Intragenic Recombination ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Double Stranded Rna ◽  
The Family ◽  
Attenuated Viruses

Intragenic recombination has been described in various RNA viruses as a mechanism to increase genetic diversity, resulting in increased virulence, expanded host range, or adaptability to a changing environment. Orbiviruses are no exception to this, with intragenic recombination previously detected in the type species, bluetongue virus (BTV). African horse sickness virus (AHSV) is a double-stranded RNA virus belonging to the Oribivirus genus in the family Reoviridae. Genetic recombination through reassortment has been described in AHSV, but not through homologous intragenic recombination. The influence of the latter on the evolution of AHSV was investigated by analyzing the complete genomes of more than 100 viruses to identify evidence of recombination. Segment-1, segment-6, segment-7, and segment-10 showed evidence of intragenic recombination, yet only one (Segment-10) of these events was manifested in subsequent lineages. The other three hybrid segments were as a result of recombination between field isolates and the vaccine derived live attenuated viruses (ALVs).

scholarly journals Double-stranded RNA virus outer shell assembly by bona fide domain-swapping

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhaoyang Sun ◽  
Kamel El Omari ◽  
Xiaoyu Sun ◽  
Serban L. Ilca ◽  
Abhay Kotecha ◽  
...  
Keyword(s):  
Rna Virus ◽  
Outer Shell ◽  
Domain Swapping ◽  
Double Stranded Rna ◽  
Bona Fide
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