scholarly journals Effects of Actin-Like Proteins Encoded by Two Bacillus pumilus Phages on Unstable Lysogeny, Revealed by Genomic Analysis

2014 ◽  
Vol 81 (1) ◽  
pp. 339-350 ◽  
Author(s):  
Yihui Yuan ◽  
Qin Peng ◽  
Dandan Wu ◽  
Zheng Kou ◽  
Yan Wu ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Protein A ◽  
Genomic Analysis ◽  
Proteome Analysis ◽  
Control Effect ◽  
Content Type ◽  
Ginger Rhizome ◽  
The Difference ◽  
Rot Disease ◽  
Rhizome Rot

ABSTRACTWe characterized two newly isolated myoviruses, Bp8p-C and Bp8p-T, infecting the ginger rhizome rot disease pathogenBacillus pumilusGR8. The plaque of Bp8p-T exhibited a clear center with a turbid rim, suggesting that Bp8p-T could transform into latent phage. Lysogeny assays showed that both the two phages could form latent states, while Bp8p-T could form latent phage at a higher frequency and stability than Bp8p-C. The genomes of Bp8p-C and Bp8p-T were 151,417 and 151,419 bp, respectively; both encoded 212 putative proteins, and only differed by three nucleotides. Moreover, owing to this difference, Bp8p-C encoded a truncated, putative actin-like plasmid segregation protein Gp27-C. Functional analysis of protein Gp27 showed that Gp27-T encoded by Bp8p-T exhibited higher ATPase activity and assembly ability than Gp27-C. The results indicate that the difference in Gp27 affected the phage lysogenic ability. Structural proteome analysis of Bp8p-C virion resulted in the identification of 14 structural proteins, among which a pectin lyase-like protein, a putative poly-gamma-glutamate hydrolase, and three proteins with unknown function, were firstly identified as components of the phage virion. Both phages exhibited specific lytic ability to the host strain GR8. Bp8p-C showed better control effect on the pathogen in ginger rhizome slices than Bp8p-T, suggesting that Bp8p-C has a potential application in bio-control of ginger rhizome rot disease.

scholarly journals Intensitas dan Luas Serangan Beberapa Isolat Fusarium oxysporum f.sp. zingiberi pada Jahe Gajah

2017 ◽  
Vol 21 (1) ◽  
pp. 16
Author(s):  
Hermawati Cahyaningrum ◽  
Nur Prihatiningsih ◽  
Soedarmono Soedarmono
Keyword(s):  
Fusarium Oxysporum ◽  
Sterile Soil ◽  
Soil Medium ◽  
Disease Symptoms ◽  
Ginger Rhizome ◽  
Incubation Periods ◽  
Rot Disease ◽  
Screen House ◽  
Rhizome Rot ◽  
Growth Ability

Ginger is one of the spices and medicinal commodities which is cultivated in Indonesia. One of the obstacles encountered in the cultivation of ginger is the rhizome rot disease which is mainly caused by Fusarium oxysporum Schlecht f.sp. zingiberi Trujillo. This study is aimed to know the growth ability and virulence level of the isolates on ginger rhizome and plants. The research was conducted in the laboratory and in the screen house by using Complete Random Design consisted of 10 treatments and 4 replications. The parameters observed were growth ability of F. oxysporum f.sp. zingiberi, rhizome rot disease symptoms, incubation period, extensive decay and weight difference of the rhizomes. The results showed that F. oxysporum f.sp. zingiberi which was stored for 4 years in sterile soil medium was still capable to cause damage to the rhizome and plants. Incubation periods of rhizome decay and plant symptoms were from 3 to 11.5 and 55.5 to 68.5 days, respectively. The most virulent isolate was MSO1 with extensive decay of rhizome and the wilting intensity were 108.95 mm2 dan 33.88%, respectively. IntisariJahe merupakan salah satu komoditas rempah dan obat yang banyak dibudidayakan di Indonesia. Salah satu kendala yang dihadapi dalam budidaya jahe adalah adanya gangguan penyakit busuk rimpang yang disebabkan (terutama) oleh Fusarium oxysporum Schlecht f.sp. zingiberi Trujillo. Penelitian bertujuan untuk menguji daya tumbuh dan virulensi isolat F. oxysporum f.sp. zingiberi pada rimpang dan tanaman jahe gajah. Penelitian dilakukan di laboratorium dan di rumah kasa menggunakan Rancangan Acak Kelompok Lengkap (RAKL) yang masing-masing terdiri dari 10 perlakuan dan 4 ulangan. Parameter yang diamati meliputi daya tumbuh F. oxysporum f.sp. zingiberi, gejala penyakit busuk rimpang, masa inkubasi, luas pembusukan dan selisih bobot basah rimpang. Hasil penelitian menunjukkan bahwa F. oxysporum f.sp. zingiberi yang telah di simpan 4 tahun dalam medium tanah steril mampu menyebabkan kerusakan pada rimpang dan tanaman jahe. Masa inkubasi gejala busuk pada rimpang serta gejala pada tanaman masing- masing berkisar antara 3–11,5 serta 55,5–68,5 hari. Isolat yang paling virulen adalah MSO1 dengan nilai luas pembusukan pada rimpang dan intensitas penyakit masing-masing sebesar 108,95 mm2 dan 33,88%.

scholarly journals Bacillus pumilus, a Novel Ginger Rhizome Rot Pathogen in China

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1308-1315 ◽  
Author(s):  
Qin Peng ◽  
Yihui Yuan ◽  
Meiying Gao
Keyword(s):  
Molecular Biology ◽  
Bacillus Pumilus ◽  
Shandong Province ◽  
Gram Positive ◽  
Disease Symptoms ◽  
Starch Grains ◽  
First Report ◽  
Ginger Rhizome ◽  
Parenchyma Cells ◽  
Rhizome Rot

Ginger rhizome rot is a major factor limiting the yield and marketability of ginger in Shandong Province, China. In order to identify the pathogen causing ginger rhizome rot, evaluate its pathogenicity, and explore its pathogenesis, diseased ginger rhizomes and surrounding soils were collected. A gram-positive, spore-forming, rod-shaped bacterium, designated GR8, was frequently isolated from the ginger rhizome samples. The bacterium was identified as Bacillus pumilus based on physio-biochemical and molecular biology characteristics. Pathogenicity studies with GR8 showed that it could cause disease of the tested rhizomes slices and the entire rhizome when wounded but no disease occurred when the rhizome was not wounded. Preliminary pathogenicity studies demonstrated that cell-free cultures of GR8 could not cause any disease symptoms, whereas the bacterial suspensions caused severe symptoms. The pathology studies revealed that infection of GR8 could cause starch grains to shrink from normal size, and destroy the parenchyma cells by invading and propagating in them. This is the first report of B. pumilus causing ginger rhizome rot.

scholarly journals Genomic, Antimicrobial, and Aphicidal Traits of Bacillus velezensis ATR2, and Its Biocontrol Potential against Ginger Rhizome Rot Disease Caused by Bacillus pumilus

2021 ◽  
Vol 10 (1) ◽  
pp. 63
Author(s):  
Leiqin Liang ◽  
Yajuan Fu ◽  
Sangsang Deng ◽  
Yan Wu ◽  
Meiying Gao
Keyword(s):  
Pathogenic Bacteria ◽  
Plant Immunity ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Gene Clusters ◽  
Bacillus Velezensis ◽  
Ginger Rhizome ◽  
Biocontrol Potential ◽  
Rot Disease ◽  
Rhizome Rot

Ginger rhizome rot disease, caused by the pathogen Bacilluspumilus GR8, could result in severe rot of ginger rhizomes and heavily threaten ginger production. In this study, we identified and characterized a new Bacillus velezensis strain, designated ATR2. Genome analysis revealed B. velezensis ATR2 harbored a series of genes closely related to promoting plant growth and triggering plant immunity. Meanwhile, ten gene clusters involved in the biosynthesis of various secondary metabolites (surfactin, bacillomycin, fengycin, bacillibactin, bacilysin, difficidin, macrolactin, bacillaene, plantazolicin, and amylocyclicin) and two clusters encoding a putative lipopeptide and a putative phosphonate which might be explored as novel bioactive compounds were also present in the ATR2 genome. Moreover, B. velezensis ATR2 showed excellent antagonistic activities against multiple plant pathogenic bacteria, plant pathogenic fungi, human pathogenic bacteria, and human pathogenic fungus. B. velezensis ATR2 was also efficacious in control of aphids. The antagonistic compound from B. velezensis ATR2 against B.pumilus GR8 was purified and identified as bacillomycin D. In addition, B. velezensis ATR2 exhibited excellent biocontrol efficacy against ginger rhizome rot disease on ginger slices. These findings showed the potential of further applications of B. velezensis ATR2 as a biocontrol agent in agricultural diseases and pests management.

scholarly journals Virulence of the Melioidosis Pathogen Burkholderia pseudomallei Requires the Oxidoreductase Membrane Protein DsbB

2018 ◽  
Vol 86 (5) ◽  
Author(s):  
Róisín M. McMahon ◽  
Philip M. Ireland ◽  
Derek S. Sarovich ◽  
Guillaume Petit ◽  
Christopher H. Jenkins ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Disulfide Bond ◽  
Disulfide Bonds ◽  
Burkholderia Pseudomallei ◽  
Protein A ◽  
Genomic Analysis ◽  
Core Gene ◽  
Content Type ◽  
Redox Biology

ABSTRACT The naturally antibiotic-resistant bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a disease with stubbornly high mortality and a complex, protracted treatment regimen. The worldwide incidence of melioidosis is likely grossly underreported, though it is known to be highly endemic in northern Australia and Southeast Asia. Bacterial disulfide bond (DSB) proteins catalyze the oxidative folding and isomerization of disulfide bonds in substrate proteins. In the present study, we demonstrate that B. pseudomallei membrane protein disulfide bond protein B (BpsDsbB) forms a functional redox relay with the previously characterized virulence mediator B. pseudomallei disulfide bond protein A (BpsDsbA). Genomic analysis of diverse B. pseudomallei clinical isolates demonstrated that dsbB is a highly conserved core gene. Critically, we show that DsbB is required for virulence in B. pseudomallei . A panel of B. pseudomallei dsbB deletion strains (K96243, 576, MSHR2511, MSHR0305b, and MSHR5858) were phenotypically diverse according to the results of in vitro assays that assess hallmarks of virulence. Irrespective of their in vitro virulence phenotypes, two deletion strains were attenuated in a BALB/c mouse model of infection. A crystal structure of a DsbB-derived peptide complexed with BpsDsbA provides the first molecular characterization of their interaction. This work contributes to our broader understanding of DSB redox biology and will support the design of antimicrobial drugs active against this important family of bacterial virulence targets.

What makes the difference? Employee social media brand engagement

2019 ◽  
Vol 34 (7) ◽  
pp. 1459-1467 ◽  
Author(s):  
Sherese Y. Duncan ◽  
Raeesah Chohan ◽  
João José Ferreira
Keyword(s):  
Social Media ◽  
Brand Equity ◽  
Positive Emotions ◽  
Content Type ◽  
Word Use ◽  
Brand Engagement ◽  
Analytical Thinking ◽  
Industry Competition ◽  
The Difference ◽  
Business To Business

Purpose This paper aims to explore, using the employee lens of business-to-business firms, word use through brand engagement and social media interaction to understand the difference between employees who rate their employer brands highly on social media and those who don't. Design/methodology/approach We conducted a textual content analysis of posts published on the social media job evaluation site glassdoor.com. LIWC software package was used to analyze 30 of the top 200 business-to-business brands listed on Brandwatch using four variables, namely, analytical thinking, clout, authenticity and emotional tone. Findings The results show that employees who rate their employer’s brand low use significantly more words, are significantly less analytic and write with significantly more clout because they focus more on others than themselves. Employees who rate their employer’s brand highly, write with significantly more authenticity, exhibit a significantly higher tone and display far more positive emotions in their reviews. Practical implications Brand managers should treat social media data disseminated by individual stakeholders, like the variables used in this study (tone, word count, frequency), as a valuable tool for brand insight on their industry, competition and their own brand equity, now and especially over time. Originality/value This study provides acknowledgement that social media is a significant source of marketing intelligence that may improve brand equity by better understanding and managing brand engagement.

scholarly journals Application of Bacillus pumilus isolates for management of black rot disease in strawberry

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Farid Abd-El-Kareem ◽  
Ibrahim E. Elshahawy ◽  
Mahfouz M. M. Abd-Elgawad
Keyword(s):  
Root Rot ◽  
Bacillus Pumilus ◽  
Field Conditions ◽  
Black Rot ◽  
Bacterial Isolates ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Growth Area ◽  
Rot Disease ◽  
The Impact

Abstract Background Black root rot of strawberry plants caused by Rhizoctonia solani, Fusarium solani, and Pythium sp. is a serious disease in Egypt. Biocontrol agents have frequently proved to possess paramount and safe tools against many diseases. The impact of soil treatments with 3 Bacillus pumilus isolates on black root rot disease of strawberry plants caused by R. solani, F., and Pythium sp. under laboratory and field conditions was examined herein on the commonly used ‘Festival’ strawberry cultivar. To increase the bacterial adhesion and distribution on the roots, each seedling was dipped in bacterial cell suspension at 1 × 108 colony-forming units/ml of each separate bacterial isolate for 30 min then mixed with 5% Arabic gum. Results The tested B. pumilus isolates significantly reduced the growth area of these 3 fungi. The two bacterial isolates Nos. 2 and 3 reduced the growth area by more than 85.2, 83.6, and 89.0% for R. solani, F. solani, and Pythium sp., respectively. Likewise, the 3 bacterial isolates significantly (P ≤ 0.05) inhibited the disease under field conditions. Isolates Nos. 2 and 3 suppressed the disease incidence by 64.4 and 68.9% and disease severity by 65.3 and 67.3%, respectively. The fungicide Actamyl had effect similar to that of the 2 isolates. B. pumilus isolates significantly enhanced growth parameters and yields of strawberry plants; isolates Nos. 2 and 3 raised the yield by 66.7 and 73.3%, respectively. Conclusions Bacillus pumilus isolates could effectively manage the black rot disease in strawberry herein. Due to the significant impact of the root rot disease on strawberry yield, B. pumilus should be further tested to manage the disease on strawberry on large scale in Egypt.

scholarly journals Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein in Leptospira interrogans Grown in EMJH Medium

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 852
Author(s):  
Abhijit Sarma ◽  
Dhandapani Gunasekaran ◽  
Devasahayam Arokia Balaya Rex ◽  
Thoduvayil Sikha ◽  
Homen Phukan ◽  
...  
Keyword(s):  
Culture Medium ◽  
Proteome Analysis ◽  
Dna Helicase ◽  
Extracellular Proteins ◽  
Surface Proteins ◽  
Secretory Proteins ◽  
Extracellular Proteome ◽  
Cell Pellet ◽  
The Difference ◽  
Leptospira Species

Leptospirosis is a re-emerging form of zoonosis that is caused by the spirochete pathogen Leptospira. Extracellular proteins play critical roles in the pathogenicity and survival of this pathogen in the host and environment. Extraction and analysis of extracellular proteins is a difficult task due to the abundance of enrichments like serum and bovine serum albumin in the culture medium, as is distinguishing them from the cellular proteins that may reach the analyte during extraction. In this study, extracellular proteins were separated as secretory proteins from the culture supernatant and surface proteins were separated during the washing of the cell pellet. The proteins identified were sorted based on the proportion of the cellular fractions and the extracellular fractions. The results showed the identification of 56 extracellular proteins, out of which 19 were exclusively extracellular. For those proteins, the difference in quantity with respect to their presence within the cell was found to be up to 1770-fold. Further, bioinformatics analysis elucidated characteristics and functions of the identified proteins. Orthologs of extracellular proteins in various Leptospira species were found to be closely related among different pathogenic forms. In addition to the identification of extracellular proteins, this study put forward a method for the extraction and identification of extracellular proteins.

Disposition effect at the market level: evidence from Indian stock market

2019 ◽  
Vol 12 (2) ◽  
pp. 69-82
Author(s):  
Sravani Bharandev ◽  
Sapar Narayan Rao
Keyword(s):  
Trading Volume ◽  
Stock Exchange ◽  
Reference Points ◽  
Disposition Effect ◽  
Content Type ◽  
Ex Post ◽  
Abnormal Trading Volume ◽  
The Difference ◽  
Trading Volumes ◽  
Indian Stock Market

Purpose The purpose of this paper is to test the disposition effect at market level and propose an appropriate reference point for testing disposition at market level. Design/methodology/approach This is an empirical study conducted on 500 index stocks of NSE500 (National Stock Exchange). Winning and losing days for each stock are calculated using 52-week high and low prices as reference points. To test disposition effect, abnormal trading volumes of stocks are regressed on their percentage of winning (losing) days. Further using ANOVA, the difference between mean of percentage of winning (losing) days of high abnormal trading volume deciles and low abnormal trading volume deciles is tested. Findings Results show that a stock’s abnormal trading volume is positively influenced by the percentage of winning days whereas percentage of losing days show no such effect. Findings are consistent even after controlling for volatility and liquidity. ANOVA results show the presence of high percentage of winning days in higher deciles of abnormal trading volumes and no such pattern in case of losing days confirms the presence of disposition effect. Further an ex post analysis indicates that disposition prone investors accumulate losses. Originality/value This is the first study, which proposes the use of 52-week high and low prices as reference points to test the market-level disposition effect. Findings of this study enhance the limited literature available on disposition effect in emerging markets by providing evidence from Indian stock markets.

Workspace, dexterity and dimensional optimization of microhexapod

2015 ◽  
Vol 35 (4) ◽  
pp. 341-347 ◽  
Author(s):  
E. Rouhani ◽  
M. J. Nategh
Keyword(s):  
Degrees Of Freedom ◽  
Optimization Problem ◽  
Screw Theory ◽  
Design Parameters ◽  
Dimensional Synthesis ◽  
Content Type ◽  
Workspace Analysis ◽  
The Difference ◽  
Flexure Joints ◽  
6 Degrees Of Freedom

Purpose – The purpose of this paper is to study the workspace and dexterity of a microhexapod which is a 6-degrees of freedom (DOF) parallel compliant manipulator, and also to investigate its dimensional synthesis to maximize the workspace and the global dexterity index at the same time. Microassembly is so essential in the current industry for manufacturing complicated structures. Most of the micromanipulators suffer from their restricted workspace because of using flexure joints compared to the conventional ones. In addition, the controllability of micromanipulators inside the whole workspace is very vital. Thus, it is very important to select the design parameters in a way that not only maximize the workspace but also its global dexterity index. Design/methodology/approach – Microassembly is so essential in the current industry for manufacturing complicated structures. Most of the micromanipulators suffer from their restricted workspace because of using flexure joints compared to the conventional ones. In addition, the controllability of micromanipulators inside the whole workspace is very vital. Thus, it is very important to select the design parameters in a way that not only maximize the workspace but also its global dexterity index. Findings – It has been shown that the proposed procedure for the workspace calculation can considerably speed the required calculations. The optimization results show that a converged-diverged configuration of pods and an increase in the difference between the moving and the stationary platforms’ radii cause the global dexterity index to increase and the workspace to decrease. Originality/value – The proposed algorithm for the workspace analysis is very important, especially when it is an objective function of an optimization problem based on the search method. In addition, using screw theory can simply construct the homogeneous Jacobian matrix. The proposed methodology can be used for any other micromanipulator.

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