High-performance thin-layer chromatographic standardization and quantification of marker compounds in an Ayurvedic polyherbal formulation: Krishnadi Churna

2022 ◽  
Author(s):  
Swati Patel ◽  
Gyanendra Singh Baghel ◽  
Nagendra Singh Chauhan ◽  
Satyawati Rathiya ◽  
Umakant Sahu ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Polyherbal Formulation ◽  
Marker Compounds

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Studies of Croton klotzschianus (Wight) Thw.

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Suresh J ◽  
Saravana Ganthi A
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Long Wavelength ◽  
Commercial Purpose ◽  
Rf Values ◽  
Marker Compounds ◽  
Ethanol Extracts ◽  
Effective Drugs ◽  
Layer Chromatography

Most of the traditional medicinal plants in India are not scientifically validated. Scientific evaluation along with traditional knowledge is essential to obtain effective drugs for commercial purpose. The present study was aimed at High Performance Thin Layer Chromatography (HPTLC) analysis of ethanol extracts of leaf, stem and root of Croton klotzschianus. The chemical fingerprint through HPTLC Studies was carried out as per the standard method. The HPTLC shows ethanolic extracts of Croton klotzschianus at UV short and long wavelength 254 and 366 using Hexane: Ethyl acetate: Toluene: Chloroform: Methanol: Formic acid (4:2.5:1.5:0.8:1:0.2) as mobile phase and the Rf values were recorded. The HPTLC fingerprint profile of leaf, stem and root extracts was exhibits 12 peaks each. The results of qualitative phytochemical screening confirm the presence of flavonoids. The HPTLC analysis to help in proper identification and quantification of marker compounds.

scholarly journals Chemical Standards and HPTLC Finger Print Profiles of a Siddha Polyherbal Formulation - Kadukkai Legiyam

2021 ◽  
Vol 12 (4) ◽  
pp. 922-926
Author(s):  
Sujith Thatipelli ◽  
Achintya Kumar Mandal ◽  
Shakila Ramachandran
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Finger Print ◽  
Polyherbal Formulation ◽  
Physico Chemical ◽  
Photo Documentation ◽  
Standard Operating ◽  
Layer Chromatography ◽  
Finger Print Patterns

To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was prepared as per the standard operating procedures mentioned in literature. Then the drug was subjected to physicochemical parameters, phytochemical screening, thin layer chromatographic photo documentation (TLC), high performance thin layer chromatographic (HPTLC) finger print profile of hexane, chloroform, ethanol and hydro alcohol (1:1) extracts.  Different extracts of the drug showed distinct TLC and HPTLC finger print patterns which will be unique to this drug. This study giving information about physiochemical and phytochemical analysis and HPTLC fingerprint profile of different extracts, the integration spectrum which will useful in standardizing the raw drugs and future comparison studies.

scholarly journals High-Performance Thin-Layer Chromatography Densitometric Method for the Quantification of Harmine, Harmaline, Vasicine, and Vasicinone in Peganum harmala

2008 ◽  
Vol 91 (5) ◽  
pp. 1179-1185 ◽  
Author(s):  
Harsha Pulpati ◽  
Yogesh S Biradar ◽  
Mandapati Rajani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Peganum Harmala ◽  
Relative Standard ◽  
Recovery Study ◽  
Marker Compounds ◽  
Layer Chromatography ◽  
Instrumental Precision ◽  
Different Levels

Abstract Peganum harmala Linn. seed is a reputed drug of the Indian systems of medicine. We report a simple high-performance thin-layer chromatography (HPTLC) densitometric method for the quantification of 4 alkaloids, viz., harmine, harmaline, vasicine, and vasicinone from P. harmala seed. The 4 compounds were resolved in ethyl acetatemethanolammonia (7 1 0.3, v/v/v) mobile phase. The method was validated for precision, repeatability, and accuracy. Instrumental precision was 0.27, 1.53, 0.39, and 1.15% [relative standard deviation (RSD)] and repeatability of the method was 1.01, 0.79, 0.13, and 1.62% RSD for harmine, harmaline, vasicine, and vasicinone, respectively. Accuracy of the method was checked by a recovery study conducted at 3 different levels, and the average recovery was 97.88% for harmine, 97.69% for harmaline, 98.38% for vasicine, and 98.28% for vasicinone. The 4 compounds were quantified in P. harmala seed using the method, and it was found to contain 0.44, 0.096, 0.25, and 0.0007% (w/w) of each, respectively. The proposed HPTLC densitometric method for the quantification of the 4 compounds was found to be simple, precise, specific, sensitive, and accurate. It can be used for routine quality control of P. harmala seed and has the ability to quantify the 4 marker compounds in the formulations containing P. harmala. It also can be used to quantify any of these marker compounds in other herbal drugs.

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