scholarly journals Chemical Standards and HPTLC Finger Print Profiles of a Siddha Polyherbal Formulation - Kadukkai Legiyam

2021 ◽  
Vol 12 (4) ◽  
pp. 922-926
Author(s):  
Sujith Thatipelli ◽  
Achintya Kumar Mandal ◽  
Shakila Ramachandran
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Finger Print ◽  
Polyherbal Formulation ◽  
Physico Chemical ◽  
Photo Documentation ◽  
Standard Operating ◽  
Layer Chromatography ◽  
Finger Print Patterns

To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was prepared as per the standard operating procedures mentioned in literature. Then the drug was subjected to physicochemical parameters, phytochemical screening, thin layer chromatographic photo documentation (TLC), high performance thin layer chromatographic (HPTLC) finger print profile of hexane, chloroform, ethanol and hydro alcohol (1:1) extracts.  Different extracts of the drug showed distinct TLC and HPTLC finger print patterns which will be unique to this drug. This study giving information about physiochemical and phytochemical analysis and HPTLC fingerprint profile of different extracts, the integration spectrum which will useful in standardizing the raw drugs and future comparison studies.

scholarly journals Physico-chemical investigation of a polyherbal formulation - Vidangatandulaadi choorna

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1728-1732
Author(s):  
Amrutha S. Babu ◽  
Mahadevan Subramaniam ◽  
Mahesh C. Kundagol ◽  
James Chacko ◽  
Devipriya Soman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Chemical Study ◽  
Inductively Coupled ◽  
Polyherbal Formulation ◽  
Physico Chemical ◽  
Plasma Mass Spectrometry ◽  
Layer Chromatography

Vidangatandulaadi Choorna is a polyherbal formulation consisting of seven ingredients. Trivrith (Operculina turpethum (Linn.) Silva Manso)  is the chief ingredient responsible for the purgative action of the formulation. This yoga is mentioned in Kalpasthana of Ashtangahridaya, intended for virechana (Purgation). It is useful in Kapha-vatha disorders. Even though many kinds of research have been done to identify the physicochemical constituents of individual drugs in the formulation, no studies were done to identify the physicochemical properties of the formulation. This analysis helps in understanding the mechanism for different pharmacological actions of the formulation. Hence, Physico-chemical study of Vidangatandulaadi Choorna along with high-performance thin-layer chromatography (HPTLC) fingerprinting is done to fix the standards. All the drugs included in the formulation is identified by the botanist and is prepared according to Standards mentioned for the preparation of Choorna mentioned in Ayurvedic Pharmacopoeia of India. The formulation is least encountered, but it has shown its significant action in Dyslipidemia in folklore practices. As there are no Standards mentioned for this formulation, the result observed in the present study may be considered suitable. The data obtained from Physicochemical investigation, high-performance thin-layer chromatography profile and ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) could be used as the standards for the present formulation under study.

scholarly journals PHARMACOGNOSTICAL STANDARDIZATION AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINT OF ROOTS OF ANTHOCEPHALUS CADAMBA

2019 ◽  
pp. 194-198
Author(s):  
KAVERIPAKAM SAISRUTHI ◽  
ADIKAY SREEDEVI
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ethanol Extract ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Vessel Elements ◽  
Layer Chromatography ◽  
Anthocephalus Cadamba

Objective: Anthocephalus cadamba is a traditional medicinal plant which is valued for its benefits in the management of various ailments. The current study was aimed to provide requisite pharmacognostic details and high-performance thin-layer chromatography (HPTLC) fingerprint profile for the roots of A. cadamba. Methods: Pharmacognostic evaluation included examination of morphological and microscopic characters, physicochemical analysis, fluorescence study, preliminary phytochemical analysis, and HPTLC fingerprint. Results: The microscopic studies showed the presence of thick periderm, cortical zone, secondary phloem, and secondary xylem. Microscopic examination of root powder showed abundant fibers and vessel elements. The physicochemical analysis of root powder helped in the assessment of quality control parameters. Fluorescence analysis of root powder imparted characteristic colors on the treatment of the powder with different reagents. Preliminary phytochemical studies reported the presence of alkaloids, saponins, flavonoids, steroids, terpenoids, glycosides, tannins, and other phenolic compounds. The developed qualitative densitometric HPTLC fingerprint can be used as an identifying biochemical marker for ethanol extract of the roots of A. cadamba. Conclusion: The present study provides the information with respect to identification and authentication of crude drug and serves as a reference point for the proper identification of roots of this medicinal plant, thereby contributing to the scientific world of research.

scholarly journals Using high performance thin layer chromatography for the detection of pharmacologically active secondary metabolites in Empetrum nigrum L.

2021 ◽  
Vol 10 (4) ◽  
pp. 129-137
Author(s):  
A. O. Ponkratova ◽  
A. K. Whaley ◽  
V. G. Luzhanin ◽  
E. V. Zhokhova
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Thin Layer ◽  
High Performance ◽  
Phytochemical Analysis ◽  
Empetrum Nigrum ◽  
Aerial Parts ◽  
Pharmacologically Active ◽  
Layer Chromatography ◽  
Derivatives Of

Introduction. The article presents the results of the detection of pharmacologically active secondary metabolites in black crowberry Empetrum nigrum L. using the method of high performance thin layer chromatography (HPTLC).Aim. To show the efficiency of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites in promising medicinal plant species.Materials and methods. HPTLC analysis was carried out on a CAMAG device (Switzerland), using MERCK HPTLC silica gel 60 F154, 20 × 10 cm plates. For the evaporation of the samples, a Heidolph vacuum rotary evaporator (Germany) was used. The aerial parts (shoots) of Empetrum nigrum were harvested next to St. Petersburg State Chemical and Pharmaceutical University (SPCPU) nursery garden of medicinal plants (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in August 2019.Results and discussion. In the course of the research, four fractions from the aerial parts of Empetrum nigrum were obtained: hexane, dichloromethane, butanol, and water. Then, these fractions were investigated by HPTLC in two solvent systems – n-butanol : acetic acid : water (BAW) (4 : 1 : 2) and hexane : dichloromethane : methanol (HDM) (1 : 2 : 0.5). After scanning densitometric analysis of the plates eluted in the HDM system, it was revealed, that the hexane and dichloromethane fractions have a similar composition and contain the greatest amount of compounds, compared to the butanol and water fractions, and in the BAW system, it was found, that the butanol fraction contains the greatest variety of metabolites. As a result of UV spectroscopy, it was found, that the main groups of compounds contained in the hexane and dichloromethane fractions are derivatives of chalcones, dihydrochalcones, bibenzyls and 9,10-dihydrophenanthrenes. While in the butanol fraction, the main groups of secondary metabolites were derivatives of flavonoids and tanninsConclusion. The data obtained allow us to note the efficiency, speed and simplicity of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites of promising medicinal plant species.

scholarly journals PHYTOCHEMICAL EVALUATION AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY PROFILING: SAPINDUS EMARGINATUS VAHL. AND MORINDA PUBESCENS J.E.SM. BARKS EXTRACTS

2016 ◽  
Vol 9 (9) ◽  
pp. 108 ◽  
Author(s):  
Hariba Dhondiram Jedage ◽  
Manjunath Kp
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Flavonoid Compound ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Rf Values ◽  
Ethanol Extracts ◽  
Morinda Pubescens ◽  
Layer Chromatography

ABSTRACTObjective: To identify the flavonoids high-performance thin layer chromatography (HPTLC) profiles from barks of Sapindus emarginatus Vahl.(Sapindaceae) and Morinda pubescens J.E.Sm. (Rubiaceae) by ethanol extracts.Methods: The barks of S. emarginatus Vahl. and M. pubescens J.E.Sm. extracted each separately with ethanol. Both these ethanol extracts weresubjected to preliminary phytochemical analysis. Based on phytochemical studies, the extracts obtained were subjected to HPTLC profiles for identifyand confirmation flavonoids, both these samples were compared with standard (Rutin). HPTLC analysis performed with silica gel G 60 F254 plateswith mobile phase ethyl acetate:n-butanol:formic acid:water (5:3:1:1). Detection of flavonoid compound was performed by scanning the developedplate at 254 nm.Results: Result of these both extracts shows positive tests for flavonoids. Ethanol extracts barks of S. emarginatus Vahl. and M. pubescens J.E.Sm eachwere showed bands of different Rf values with range 0.50-0.90 and standard showed bands of 0.50 Rf values.Conclusion: It can be concluded that rutin constituents of each bark extract are presents and have effective components which can be utilized as auseful herb for alleviation of various illness and disorders.Keywords: Sapindus emarginatus Vahl., Morinda pubescens J.E.Sm. Ethanol extracts, High-performance thin layer chromatography, Rutin.

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