scholarly journals Evaluation of a digital microfluidic real-time PCR platform to detect DNA of Candida albicans in blood

2012 ◽  
Vol 31 (9) ◽  
pp. 2237-2245 ◽  
Author(s):  
W. A. Schell ◽  
J. L. Benton ◽  
P. B. Smith ◽  
M. Poore ◽  
J. L. Rouse ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Digital Microfluidic

scholarly journals Use of Quantitative Real-Time PCR To Study the Kinetics of Extracellular DNA Released from Candida albicans, with Implications for Diagnosis of Invasive Candidiasis

2006 ◽  
Vol 44 (1) ◽  
pp. 143-150 ◽  
Author(s):  
M. Kasai ◽  
A. Francesconi ◽  
R. Petraitiene ◽  
V. Petraitis ◽  
A. M. Kelaher ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Invasive Candidiasis ◽  
Real Time Pcr ◽  
Extracellular Dna ◽  
Quantitative Real Time Pcr ◽  
Kinetics Of

scholarly journals Rapid Screening Method for Compounds That Affect the Growth and Germination of Candida albicans, Using a Real-Time PCR Thermocycler

2011 ◽  
Vol 77 (22) ◽  
pp. 8193-8196 ◽  
Author(s):  
Lucja M. Jarosz ◽  
Bastiaan P. Krom
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Fluorescent Protein ◽  
Screening Method ◽  
Rapid Screening ◽  
Content Type ◽  
Wide Range ◽  
Hyphal Formation ◽  
Green Fluorescent

ABSTRACTWe propose a screening method for compounds affecting growth and germination inCandida albicansusing a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using PACT1-GFPand PHWP1-GFPreporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.

scholarly journals Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression

2010 ◽  
Vol 10 (1) ◽  
pp. 114 ◽  
Author(s):  
Heleen Nailis ◽  
Soňa Kucharíková ◽  
Markéta Řičicová ◽  
Patrick Van Dijck ◽  
Dieter Deforce ◽  
...  
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Real Time ◽  
Virulence Factors ◽  
Real Time Pcr ◽  
Expression Profiling ◽  
Independent Gene ◽  
Genes Encoding ◽  
Candida Albicans Biofilms

scholarly journals Effects of Bacillus subtilis on Candida albicans: biofilm formation, filamentation and gene expression

2019 ◽  
Vol 22 (2) ◽  
pp. 252-259
Author(s):  
Michelle Peneluppi Silva ◽  
Patrícia Pimentel De Barros ◽  
Adeline Lacerda Jorjão ◽  
Rodnei Dennis Rossoni ◽  
Juliana Campos Junqueira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Bovine Serum ◽  
Biofilm Formation ◽  
Fetal Bovine Serum ◽  
Viable Cells ◽  
Fetal Bovine

Objective: The aim of this study was evaluate the effect of Bacillus subtilis on Candida albicans biofilm formation and filamentation by evaluating the gene expression of ALS3, HWP1, BCR1, EFG1 and TEC1. Material and Methods: Mixed (C. albicans / B.subtilis) and monotypic biofilms were cultured in plates at 37°C for 48 h under shaking for counting viable cells (CFU / mL) and analysis of gene expression by real-time PCR. The C. albicans filamentation assay was performed in medium containing 10% fetal bovine serum at 37°C for 6 hours. Data was analysed by t-Student and Mann– Whitney tests. Results: B. subtilis reduced the biofilm formation of C. albicans in 1 log when cultured in the same environment (p<0.0001). In addition, it significantly reduced the yeast -hypha transition affecting the morphology of C. albicans. Among all of the analyzed genes, the ALS3 and HWP1 genes were the most affected, achieving 111.1- and 333.3- fold decreases in the C. albicans biofilms associated with B. subtilis, respectively. Conclusion: B. subtilis reduced the biofilm formation and filamentation of C. albicans by negatively regulating the ALS3, HWP1, BCR1, EFG1 and TEC1 genes that are essential for the production of biofilm and hyphae.KeywordsBacillus subtilis; Candida albicans; Biofilm; Filamentation; Gene expression.

scholarly journals A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood

2020 ◽  
Vol 62 ◽  
Author(s):  
Felipe Delatorre Busser ◽  
Vivian Caso Coelho ◽  
Claudia de Abreu Fonseca ◽  
Gilda Maria Barbaro Del Negro ◽  
Maria Aparecida Shikanai-Yasuda ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Human Blood ◽  
Real Time Pcr ◽  
Detection And Quantification

Use of 65kDa mannoprotein gene primers in Real Time PCR identification of Candida albicans in biological samples

2006 ◽  
Vol 20 (5) ◽  
pp. 263-268 ◽  
Author(s):  
Silvia Arancia ◽  
Alessandra Carattoli ◽  
Roberto La Valle ◽  
Antonio Cassone ◽  
Flavia De Bernardis
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Biological Samples ◽  
Pcr Identification

scholarly journals Clinical evaluation of the DermaGenius® Nail real-time PCR assay for the detection of dermatophytes and Candida albicans in nails

2018 ◽  
Vol 57 (3) ◽  
pp. 277-283 ◽  
Author(s):  
Marie-Pierre Hayette ◽  
Laurence Seidel ◽  
Caroline Adjetey ◽  
Rajae Darfouf ◽  
Marie Wéry ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Clinical Evaluation ◽  
Real Time Pcr ◽  
Pcr Assay

P1863 Specific detection of Candida albicans using real-time PCR on the LightCycler™

2007 ◽  
Vol 29 ◽  
pp. S533
Author(s):  
L. O'Connor ◽  
M. Ruttledge ◽  
M. Maher
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae
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