Effect of Focal Adhesion Proteins on Endothelial Cell Adhesion, Motility and Orientation Response to Cyclic Strain

2009 ◽  
Vol 38 (1) ◽  
pp. 208-222 ◽  
Author(s):  
Hai Ngu ◽  
Yunfeng Feng ◽  
Lan Lu ◽  
Sara J. Oswald ◽  
Gregory D. Longmore ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Focal Adhesion ◽  
Cyclic Strain ◽  
Adhesion Proteins ◽  
Focal Adhesion Proteins ◽  
Orientation Response ◽  
Endothelial Cell Adhesion

Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain

1996 ◽  
Vol 271 (2) ◽  
pp. C635-C649 ◽  
Author(s):  
Y. Yano ◽  
J. Geibel ◽  
B. E. Sumpio
Keyword(s):  
Endothelial Cells ◽  
Morphological Change ◽  
Tyrosine Phosphorylation ◽  
Focal Adhesion ◽  
Cyclic Strain ◽  
Aortic Endothelial Cells ◽  
Adhesion Proteins ◽  
Focal Adhesion Proteins ◽  
And Migration ◽  
Aortic Endothelial

The objective of this study was to determine whether focal adhesion proteins pp125FAK (focal adhesion kinase) and paxillin are phosphorylated on tyrosine and might play a role in the morphological change and cell migration induced by strain. Bovine aortic endothelial cells (EC) were subjected to 10% average strain at 60 cycles/min. Cyclic strain increased the tyrosine phosphorylation of pp125FAK at 30 min (3.4-fold) and 4 h (5.9-fold) and the tyrosine phosphorylation of paxillin at 4 h (2.0-fold). Confocal microscopy showed that, after 4-h exposure to strain, EC began to elongate and F-actin, pp125FAK, and paxillin aligned, although they randomly distributed in static condition. Tyrosine kinase inhibitor tyrphostin A25 (100 microM) inhibited not only the tyrosine phosphorylation of pp125FAK and paxillin but also the redistribution of pp125FAK and paxillin, morphological change, and migration of EC induced by strain. These data demonstrate that cyclic strain induced tyrosine phosphorylation and reorganization of pp125FAK and paxillin and suggest that these focal adhesion proteins play a specific role in cyclic strain-induced morphological change and migration.

scholarly journals MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis

2021 ◽  
Author(s):  
Begoña Alday-Parejo ◽  
Kedar Ghimire ◽  
Oriana Coquoz ◽  
Gioele W. Albisetti ◽  
Luca Tamò ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Focal Adhesion ◽  
Endothelial Cell Adhesion

scholarly journals CAIR-1/BAG-3 modulates cell adhesion and migration by downregulating activity of focal adhesion proteins

2006 ◽  
Vol 312 (15) ◽  
pp. 2962-2971 ◽  
Author(s):  
Jareer N. Kassis ◽  
Elizabeth A. Guancial ◽  
Howard Doong ◽  
Victoria Virador ◽  
Elise C. Kohn
Keyword(s):  
Cell Adhesion ◽  
Focal Adhesion ◽  
Adhesion Proteins ◽  
Focal Adhesion Proteins ◽  
Cell Adhesion And Migration ◽  
And Migration
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