scholarly journals Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues

2020 ◽  
Vol 48 (8) ◽  
pp. 2218-2232
Author(s):  
Jessica S. Sadick ◽  
Lorin A. Crawford ◽  
Harry C. Cramer ◽  
Christian Franck ◽  
Shane A. Liddelow ◽  
...  
Keyword(s):  
Specific Protein ◽  
Cell Type ◽  
Protein Signatures ◽  
Cell Type Specific

scholarly journals Btbd11 is an inhibitory interneuron specific synaptic scaffolding protein that supports excitatory synapse structure and function

2021 ◽  
Author(s):  
Alexei M. Bygrave ◽  
Ayesha Sengupta ◽  
Ella P. Jackert ◽  
Mehroz Ahmed ◽  
Beloved Adenuga ◽  
...  
Keyword(s):  
Inhibitory Interneuron ◽  
Nmda Receptor Antagonist ◽  
Specific Protein ◽  
Network Activity ◽  
Scaffolding Protein ◽  
Inhibitory Interneurons ◽  
Cell Type ◽  
Cell Type Specific

Synapses in the brain exhibit cell–type–specific differences in basal synaptic transmission and plasticity. Here, we evaluated cell–type–specific differences in the composition of glutamatergic synapses, identifying Btbd11, as an inhibitory interneuron–specific synapse–enriched protein. Btbd11 is highly conserved across species and binds to core postsynaptic proteins including Psd–95. Intriguingly, we show that Btbd11 can undergo liquid–liquid phase separation when expressed with Psd–95, supporting the idea that the glutamatergic post synaptic density in synapses in inhibitory and excitatory neurons exist in a phase separated state. Knockout of Btbd11 from inhibitory interneurons decreased glutamatergic signaling onto parvalbumin–positive interneurons. Further, both in vitro and in vivo, we find that Btbd11 knockout disrupts network activity. At the behavioral level, Btbd11 knockout from interneurons sensitizes mice to pharmacologically induced hyperactivity following NMDA receptor antagonist challenge. Our findings identify a cell–type–specific protein that supports glutamatergic synapse function in inhibitory interneurons–with implication for circuit function and animal behavior.

scholarly journals LIM Factor Lhx3 Contributes to the Specification of Motor Neuron and Interneuron Identity through Cell-Type-Specific Protein-Protein Interactions

Cell ◽  
2002 ◽  
Vol 110 (2) ◽  
pp. 237-249 ◽  
Author(s):  
Joshua P. Thaler ◽  
Soo-Kyung Lee ◽  
Linda W. Jurata ◽  
Gordon N. Gill ◽  
Samuel L. Pfaff
Keyword(s):  
Motor Neuron ◽  
Protein Interactions ◽  
Specific Protein ◽  
Cell Type ◽  
Protein Protein Interactions ◽  
Cell Type Specific

A genetically encodable cell-type-specific protein synthesis inhibitor

2019 ◽  
Vol 16 (8) ◽  
pp. 699-702 ◽  
Author(s):  
Maximilian Heumüller ◽  
Caspar Glock ◽  
Vidhya Rangaraju ◽  
Anne Biever ◽  
Erin M. Schuman
Keyword(s):  
Protein Synthesis ◽  
Specific Protein ◽  
Protein Synthesis Inhibitor ◽  
Cell Type ◽  
Synthesis Inhibitor ◽  
Cell Type Specific

scholarly journals In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots

Nature ◽  
2017 ◽  
Vol 548 (7665) ◽  
pp. 97-102 ◽  
Author(s):  
Yuchen Long ◽  
Yvonne Stahl ◽  
Stefanie Weidtkamp-Peters ◽  
Marten Postma ◽  
Wenkun Zhou ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Specific Protein ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145451 ◽  
Author(s):  
Anke Müller ◽  
Anne Stellmacher ◽  
Christine E. Freitag ◽  
Peter Landgraf ◽  
Daniela C. Dieterich
Keyword(s):  
Specific Protein ◽  
Protein Labeling ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals The chromatin-regulating CoREST complex is animal specific and essential for development in the cnidarian Nematostella vectensis

2021 ◽  
Author(s):  
James M Gahan ◽  
Maria Hernandez-Valladares ◽  
Fabian Rentzsch
Keyword(s):  
Cell Differentiation ◽  
Regulatory Networks ◽  
Specific Protein ◽  
Nematostella Vectensis ◽  
Cell Type ◽  
Cnidarian Nematostella Vectensis ◽  
Key Players ◽  
A Cell ◽  
Cell Type Specific ◽  
Chromatin Modifying Complex

Chromatin-modifying proteins are key players in the regulation of development and cell differentiation in animals. Many individual chromatin modifiers, however, predate the evolution of animal multicellularity and how they became integrated into the regulatory networks underlying development is unclear. Here we show that CoREST is an animal-specific protein that assembles a conserved, vertebrate-like histone-modifying complex including Lsd1 and HDAC1/2 in the sea anemone Nematostella vectensis. We further show that NvCoREST expression overlaps fully with that of NvLsd1 throughout development. NvCoREST mutants, generated using CRISPR-Cas9, reveal essential roles during development and for the differentiation of cnidocytes, thereby phenocopying NvLsd1 mutants. We also show that this requirement is cell autonomous using a cell-type-specific rescue approach. Together, this shows that the evolution of CoREST allowed the formation of a chromatin-modifying complex that was present before the last common cnidarian-bilaterian ancestor and thus represents an ancient component of the animal developmental toolkit.

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