Muscular Thin Films for Label-Free Mapping of Excitation Propagation in Cardiac Tissue

AbstractCapacitive field-effect electrolyte-diamond-insulator-semiconductor (EDIS) structures with O-terminated nanocrystalline diamond (NCD) as sensitive gate material have been realized and investigated for the detection of pH, penicillin concentration, and layer-by-layer adsorption of polyelectrolytes. The surface oxidizing procedure of NCD thin films as well as the seeding and NCD growth process on a Si-SiO2 substrate have been improved to provide high pH-sensitive, non-porous thin films without damage of the underlying SiO2 layer and with a high coverage of O-terminated sites. The NCD surface topography, roughness, and coverage of the surface groups have been characterized by SEM, AFM and XPS methods. The EDIS sensors with O-terminated NCD film treated in oxidizing boiling mixture for 45 min show a pH sensitivity of about 50 mV/pH. The pH-sensitive properties of the NCD have been used to develop an EDIS-based penicillin biosensor with high sensitivity (65-70 mV/decade in the concentration range of 0.25-2.5 mM penicillin G) and low detection limit (5 μM). The results of label-free electrical detection of layer-by-layer adsorption of charged polyelectrolytes are presented, too.

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Label - free fluorescence lifetime imaging of microfluidic device based cardiac tissue model metabolism

Frontiers in Optics 2014 ◽

10.1364/fio.2014.jw3a.12 ◽

2014 ◽

Author(s):

Rupsa Datta ◽

Christopher Heylman ◽

David Tran ◽

Steven C. George ◽

Enrico Gratton

Keyword(s):

Microfluidic Device ◽

Fluorescence Lifetime ◽

Cardiac Tissue ◽

Fluorescence Lifetime Imaging ◽

Label Free ◽

Tissue Model ◽

Lifetime Imaging

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Plasmonics for Biosensing

Materials ◽

10.3390/ma12091411 ◽

2019 ◽

Vol 12 (9) ◽

pp. 1411 ◽

Cited By ~ 15

Author(s):

Xue Han ◽

Kun Liu ◽

Changsen Sun

Keyword(s):

Thin Films ◽

Molecular Level ◽

Point Of Care ◽

High Sensitivity ◽

Short Review ◽

Dynamic Monitoring ◽

Label Free ◽

Plasmonic Resonance ◽

Hybrid Functions ◽

Resonance Modes

Techniques based on plasmonic resonance can provide label-free, signal enhanced, and real-time sensing means for bioparticles and bioprocesses at the molecular level. With the development in nanofabrication and material science, plasmonics based on synthesized nanoparticles and manufactured nano-patterns in thin films have been prosperously explored. In this short review, resonance modes, materials, and hybrid functions by simultaneously using electrical conductivity for plasmonic biosensing techniques are exclusively reviewed for designs containing nanovoids in thin films. This type of plasmonic biosensors provide prominent potential to achieve integrated lab-on-a-chip which is capable of transporting and detecting minute of multiple bio-analytes with extremely high sensitivity, selectivity, multi-channel and dynamic monitoring for the next generation of point-of-care devices.

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Label‐free superresolution effect of nonlinear reverse saturation absorption thin films through difference method

Journal of Microscopy ◽

10.1111/jmi.12838 ◽

2019 ◽

Vol 276 (2) ◽

pp. 82-88

Author(s):

C. DING ◽

J. WEI

Keyword(s):

Thin Films ◽

Difference Method ◽

Label Free ◽

Reverse Saturation Absorption ◽

Saturation Absorption

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Curvature-dependent excitation propagation in cultured cardiac tissue

JETP Letters ◽

10.1134/s0021364011230044 ◽

2012 ◽

Vol 94 (11) ◽

pp. 824-830 ◽

Cited By ~ 9

Author(s):

S. Kadota ◽

M. W. Kay ◽

N. Magome ◽

K. Agladze

Keyword(s):

Cardiac Tissue ◽

Excitation Propagation

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A label-free method based on MALDI-TOF mass spectrometry for the absolute quantitation of troponin T in mouse cardiac tissue

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-008-2113-x ◽

2008 ◽

Vol 391 (5) ◽

pp. 1969-1976 ◽

Cited By ~ 14

Author(s):

Mariano Bizzarri ◽

Chiara Cavaliere ◽

Patrizia Foglia ◽

Chiara Guarino ◽

Roberto Samperi ◽

...

Keyword(s):

Mass Spectrometry ◽

Cardiac Tissue ◽

Troponin T ◽

Label Free ◽

Absolute Quantitation ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

The Absolute

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A Review of Refractometric Sensors Based on Long Period Fibre Gratings

The Scientific World JOURNAL ◽

10.1155/2013/913418 ◽

2013 ◽

Vol 2013 ◽

pp. 1-14 ◽

Cited By ~ 45

Author(s):

G. Rego

Keyword(s):

Thin Films ◽

Refractive Index ◽

Transition Region ◽

Food Quality ◽

Scientific Community ◽

Surrounding Medium ◽

Quality And Safety ◽

Label Free ◽

Long Period ◽

Food Quality And Safety

In the last decade refractometric sensors have attracted an increasing interest by the scientific community due to their ability to perform ambient monitoring, to assess food quality and safety, and also to the fact that they enable the development of label free sensors in the biomedical area. These advances result, namely, from the use of long period fibre gratings in the turning points and/or with thin films in the transition region that allows resolutions of 10−6to changes in the refractive index of the surrounding medium. Resolutions exceeding 10−8can also be achieved when long period fibre gratings are combined with evanescent field based devices. This paper reviews the recent path towards the development of ultrahigh sensitive optical fibre refractometric sensors.

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Cardiac Atrial Compartmentalisation Proteomics: A Modified Density Gradient Method to Analyse Endo-lysosomal Proteins

10.1101/2021.02.22.432193 ◽

2021 ◽

Author(s):

Thamali Ayagama ◽

Samuel J Bose ◽

Rebecca A Capel ◽

David A Priestman ◽

Georgina Berridge ◽

...

Keyword(s):

Density Gradient ◽

Cardiac Tissue ◽

Calcium Signalling ◽

Rab Proteins ◽

Label Free ◽

Enzyme Activity Assay ◽

Differential Density ◽

Tissue Lysate ◽

Good Coverage ◽

Lysosomal Proteins

SummaryThe importance of lysosomes in cardiac physiology and pathology are well established, and evidence for roles in calcium signalling are emerging. We describe a label-free proteomics method suitable for small cardiac tissue biopsies based on density-separated fractionation, which allows study of endo-lysosomal (EL) proteins.Density gradient fractions corresponding to tissue lysate; sarcoplasmic reticulum (SR), mitochondria (Mito) (1.3 g/ml); and EL with negligible contamination from SR or Mito (1.04 g/ml), were analysed using Western Blot, enzyme activity assay and LC-MS/MS analysis (adapted discontinuous Percoll, and sucrose differential density gradient).Kyoto Encyclopedia of Genes and Genomes, Reactome, Panther and Gene Ontology pathway analysis showed good coverage of RAB proteins and lysosomal cathepsins (including cardiac-specific cathepsin D) in the purified EL fraction. Significant EL proteins recovered included catalytic activity proteins. We thus present a comprehensive protocol and dataset of guinea-pig atrial EL organelle proteomics using techniques also applicable for non-cardiac tissue.

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