Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Objectives: Paris polyphylla Sm. is a very important anti-cancerous plant species found in the Himalayan region of India. The present study was carried out to determine the phenolic and flavonoid content, antioxidant and antimicrobial activity of its rhizome.Methods: Antioxidant activity of rhizome extract was evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Antimicrobial activity was evaluated through disc diffusion assay against two bacterial and two fungal strains. The bacterial species used in the present study were Escherichia coli and Staphylococcus aureus and the fungal strains used were Aspergillus niger and Trichoderma reesei. Results: The standard curve of Gallic acid revealed that the phenolic content of our sample is 43.01±0.17 mg Gallic acid equivalents (GAE)/g dry weight (DW). Similarly, flavonoids were obtained as 28±0.12 mg quercetin equivalent/g DW of the sample. It was observed that the methanolic rhizome extract showed higher antioxidant potential than water extract with the IC50 value of 1.09 mg/ml. Further, the rhizome extract of P. polyphylla species exhibited significant antimicrobial activity and it was observed that at concentration of 5 mg/ml of the sample, the percentage inhibition was 95-97% in E. coli, S. aureus and A. niger whereas in case of T. reesei it was 74%.Conclusion: This study is first of its kind in the Indian subcontinent on this plant species and these findings indicate the tremendous and promising potential of this wonder herb. A lot of work has already been done in China on this species, therefore, further studies are required for the conservation and sustainable use in Indian subcontinent before this species is thrown into extinction as it has already been declared vulnerable.

Download Full-text

Paenibacillus paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.003997 ◽

2020 ◽

Vol 70 (3) ◽

pp. 1940-1946 ◽

Cited By ~ 5

Author(s):

Min Wang ◽

Xing-Wang Jiang ◽

Shu-Kun Tang ◽

Xiao-Yang Zhi ◽

Ling-Ling Yang

Keyword(s):

Type Species ◽

Sequence Similarity ◽

Bacterial Species ◽

Cellular Fatty Acid ◽

Diaminopimelic Acid ◽

Rrna Gene ◽

Respiratory Quinone ◽

Content Type ◽

Link Type ◽

Paris Polyphylla

A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var. yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization. It grew optimally with 0–1 % NaCl (w/v), at pH 7 and at 30 °C. The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cellular fatty acid was anteiso-C15 : 0. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %). The digital DNA–DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6–53.3 % and 79.9–93.6 %. The G+C content of the genomic DNA was 47.7 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus , for which the name Paenibacillus paridis sp. nov. is proposed. The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).

Download Full-text

Chryseobacterium paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

10.21203/rs.3.rs-526912/v1 ◽

2021 ◽

Author(s):

Zhen Zhang ◽

Ling-Ling Yang ◽

Cong-Jian Li ◽

Xing-Wang Jiang ◽

Xiao-Yang Zhi

Keyword(s):

Type Strain ◽

Novel Species ◽

Phylogenetic Analyses ◽

Bacterial Species ◽

Rrna Gene ◽

Major Polar Lipid ◽

Isoprenoid Quinone ◽

16S Rrna Gene Sequences ◽

Gram Negative ◽

Paris Polyphylla

Abstract A Gram-negative, yellow-pigmented, rod-shaped bacterial strain YIM B02567 T was isolated from the root of Paris polyphylla Smith var. yunnanensis in China. Strain YIM B02567 T grew optimally at 25–30°C and at pH 7.0 in the absence of NaCl on nutrient agar. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YIM B02567 T belong to the genus Chryseobacterium , and closely related to Chryseobacterium piperi CTM T and Chryseobacterium soli DSM 19298 T . Whole genome sequencing indicated that the genome size was 4,774,612 bp and had a DNA G+C content of 34.5 %. Values of the ANI and the dDDH between strain YIM B02567 T and its closely related Chryseobacterium species were below 81.72 % and 24.7 %. Strain YIM B02567 T contained menaquinone-6 as the sole isoprenoid quinone, anteiso-C 15:0, iso-C 17:1 ω 9 c and iso-C 17:0 3-OH as major fatty acids and phosphatidylethanolamine as major polar lipid. Based on the polyphasic analyses, strain YIM B02567 T could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium . The isolate therefore represents a novel species, for which the name Chryseobacterium paridis sp. nov. is proposed. The type strain is YIM B02567 T (=CGMCC 1.18657 T =JCM 5426 T ).

Download Full-text

Chryseobacterium paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Archives of Microbiology ◽

10.1007/s00203-021-02504-2 ◽

2021 ◽

Author(s):

Zhen Zhang ◽

Ling-Ling Yang ◽

Cong-Jian Li ◽

Xing-Wang Jiang ◽

Xiao-Yang Zhi

Keyword(s):

Bacterial Species ◽

Paris Polyphylla

Download Full-text

Structural analysis of the photosynthetic membrane from Ectothiorhodospira halochloris

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007727x ◽

1983 ◽

Vol 41 ◽

pp. 740-741

Author(s):

H. Engelhardt ◽

R. Guckenberger ◽

W. Baumeister

Keyword(s):

Lattice Structure ◽

Bacterial Species ◽

Hexagonal Lattice ◽

Reaction Centre ◽

Photosynthetic Membrane ◽

Rhodopseudomonas Viridis ◽

Photosynthetic Membranes ◽

Ectothiorhodospira Halochloris ◽

Main Components

Bacterial photosynthetic membranes contain, apart from lipids and electron transport components, reaction centre (RC) and light harvesting (LH) polypeptides as the main components. The RC-LH complexes in Rhodopseudomonas viridis membranes are known since quite seme time to form a hexagonal lattice structure in vivo; hence this membrane attracted the particular attention of electron microscopists. Contrary to previous claims in the literature we found, however, that 2-D periodically organized photosynthetic membranes are not a unique feature of Rhodopseudomonas viridis. At least five bacterial species, all bacteriophyll b - containing, possess membranes with the RC-LH complexes regularly arrayed. All these membranes appear to have a similar lattice structure and fine-morphology. The lattice spacings of the Ectothiorhodospira haloohloris, Ectothiorhodospira abdelmalekii and Rhodopseudomonas viridis membranes are close to 13 nm, those of Thiocapsa pfennigii and Rhodopseudomonas sulfoviridis are slightly smaller (∼12.5 nm).

Download Full-text