scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF PARIS POLYPHYLLA SM.

2017 ◽  
Vol 10 (11) ◽  
pp. 315 ◽  
Author(s):  
Homsingli Mayirnao ◽  
Arbeen Ahmad Bhat
Keyword(s):  
Antimicrobial Activity ◽  
Gallic Acid ◽  
Plant Species ◽  
Indian Subcontinent ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Water Extract ◽  
Fungal Strains ◽  
Paris Polyphylla ◽  
Antioxidant And Antimicrobial Activity

  Objectives: Paris polyphylla Sm. is a very important anti-cancerous plant species found in the Himalayan region of India. The present study was carried out to determine the phenolic and flavonoid content, antioxidant and antimicrobial activity of its rhizome.Methods: Antioxidant activity of rhizome extract was evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Antimicrobial activity was evaluated through disc diffusion assay against two bacterial and two fungal strains. The bacterial species used in the present study were Escherichia coli and Staphylococcus aureus and the fungal strains used were Aspergillus niger and Trichoderma reesei. Results: The standard curve of Gallic acid revealed that the phenolic content of our sample is 43.01±0.17 mg Gallic acid equivalents (GAE)/g dry weight (DW). Similarly, flavonoids were obtained as 28±0.12 mg quercetin equivalent/g DW of the sample. It was observed that the methanolic rhizome extract showed higher antioxidant potential than water extract with the IC50 value of 1.09 mg/ml. Further, the rhizome extract of P. polyphylla species exhibited significant antimicrobial activity and it was observed that at concentration of 5 mg/ml of the sample, the percentage inhibition was 95-97% in E. coli, S. aureus and A. niger whereas in case of T. reesei it was 74%.Conclusion: This study is first of its kind in the Indian subcontinent on this plant species and these findings indicate the tremendous and promising potential of this wonder herb. A lot of work has already been done in China on this species, therefore, further studies are required for the conservation and sustainable use in Indian subcontinent before this species is thrown into extinction as it has already been declared vulnerable.

scholarly journals HPLC Evaluation of Phenolic Profile, Nutritive Content, and Antioxidant Capacity of Extracts Obtained fromPunica granatumFruit Peel

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Sushil Kumar Middha ◽  
Talambedu Usha ◽  
Veena Pande
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Scavenging Activity ◽  
Fruit Peel ◽  
Phenolic Profile ◽  
Antioxidant Power

This study revealed polyphenolic content, nutritive content, antioxidant activity, and phenolic profile of methanol and aqueous extracts ofPunica granatumpeel extract. For this, extracts were screened for possible antioxidant activities by free radical scavenging activity (DPPH), hydrogen peroxide scavenging activity and ferric-reducing antioxidant power (FRAP) assays. The total phenolics and flavonoid recovered by methanolic (MPE) and the water extract (AQPE) were ranged from 185 ± 12.45 to 298.00 ± 24.86 mg GAE (gallic acid equivalents)/gm and 23.05 ± 1.54 to 49.8 ± 2.14 quercetin (QE) mg/g, respectively. The EC50of herbal extracts ranged from 100 µg/ml (0.38 quercetin equivalents), for AQPE, 168 µg/ml (0.80 quercetin equivalents), for MPE. The phenolic profile in the methanolic extracts was investigated by chromatographic (HPLC) method. About 5 different flavonoids, phenolic acids, and their derivatives including quercetin (1), rutin (2), gallic acid (3), ellagic acid (4), and punicalagin as a major ellagitannin (5) have been identified. Among both extracts, methanolic extract was the most effective. This report may be the first to show nutritive content and correlation analysis to suggest that phenols and flavonoids might contribute the high antioxidant activity of this fruit peel and establish it as a valuable natural antioxidant source applicable in the health food industry.

scholarly journals Cytotoxic, antioxidant, and antimicrobial activities of Celery (Apium graveolens L.)

2021 ◽  
Vol 17 (1) ◽  
pp. 147-156
Author(s):  
Mohammed Saleh Al Aboody ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antihypertensive Agents ◽  
Apium Graveolens ◽  
Cell Protein ◽  
Dilution Method ◽  
Local Market ◽  
Antioxidant And Antimicrobial Activity

Celery (Apium graveolens Linn, Family: Apiaceae) is a common edible herb used as a spice in the traditional medicine of several nations since time immemorial. The whole plant is extensively used in cooking as soups and salads. A. graveolens has various pharmacological properties such as anticancer, anti-obesity, anti-hepatotoxic, and antihypertensive agents. Hence, it is of interest to document the in vitro cytotoxic, antioxidant, and antimicrobial activity of A. graveolens. The plants were collected in the local market, shade dried, and different parts of the plants were extracted with 70% ethanol using a cold maceration process. Antioxidant tests were performed based on the various radical scavenging methods. Antimicrobial activity and MIC were completed using the respective cup-plate and two-fold serial dilution method. In vitro cytotoxic studies were achieved by the MTT; Sulphorhodamine B assayed total cell protein content. DLA and ESC cells determined the short-term toxicity. The leaf extract exhibited significant antioxidant properties against NO, DPPH, ABTS, LPO, and HPO methods. Thus, potential inhibition against Gram-positive, Gram-negative, and fungal strains within the MIC ranges of 250-500 μg/ml was observed. All the extracts of the plant presented in the study revealed greater cytotoxicity effects against five respective cancer cell lines, L6, Vero, BRL 3A, A-549, L929, and L-929 with the ranging of 443-168.5 μg/ml. Thus, we show that A. graveolens possess a potential cytotoxic, antioxidant, and antimicrobial activity.

scholarly journals ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF BIOACTIVE CHITOSAN FILMS

2022 ◽  
Vol 52 (1) ◽  
pp. 61-65
Author(s):  
Maria Belen Vignola ◽  
Matias Alejandro Raspo ◽  
Cesar Gerardo Gómez ◽  
Alfonsina Ester Andreatta
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Salicylic Acid ◽  
Gallic Acid ◽  
Tween 80 ◽  
Food Preservation ◽  
The Other ◽  
Food Packages ◽  
Chitosan Films ◽  
Antioxidant And Antimicrobial Activity

Interest in the development of films for food preservation is increasing due to the improvement achieved in the food preservation time with the advent of new packaging technologies. Chitosan is a widely used biopolymer produced from the deacetylation of chitin, which has a good capacity to form suitable films as food packages. On the other hand, bioactive compounds such as gallic acid and salicylic acid act as inhibitors of the oxidant activity of free radicals and delay the decomposition of food. In this sense, the preparation of chitosan films modified with the mixture incorporation of salicylic acid/sorbitol or gallic acid/Tween 80 was proposed, using sorbitol or Tween 80 as plasticizers. Both films (chitosan/gallic acid/Tween 80 and chitosan/salicylic acid/sorbitol) showed a good antioxidant capacity in while the chitosan/gallic acid/Tween 80 film evidenced its antimicrobial activity against Escherichia coli ATCC 25922 causing a diminution of unit forming colony

Comparative study of antioxidant and antimicrobial activity of different parts of lemongrass leaves and their application in the functional drink

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Muhammad Abbas Ranjah ◽  
Amir Ismail ◽  
Muhammad Waseem ◽  
Saira Tanweer ◽  
Baila Ahmad ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Food Products ◽  
Content Type ◽  
Methanolic Extracts ◽  
Antioxidant And Antimicrobial Activity ◽  
Functional Drink ◽  
Different Parts

Purpose This study aims to compare the antioxidant and antimicrobial activity of different parts (tip, mid and base portion) of lemongrass leaves for application as a natural ingredient in the functional drink. Design/methodology/approach Lemongrass leaf powder was prepared from different parts of leaves and evaluated for nutritional composition. Additionally, the extracts of different portions of lemongrass leaves were analyzed for total phenolics, free radical scavenging activities, ferric reducing antioxidant potential (FRAP) and antimicrobial activities for their application in food products. Findings Tip portion of lemongrass leaf anticipated significantly (p < 0.05) higher contents of ash, protein, calcium, potassium and iron i.e. 6.2 mg/100 g, 18 mg/100 g, 340 ppm, 819 ppm and 32 ppm, respectively. Maximum (p < 0.05) phenolics (14.7 mg GAE/100 g), 2,2-diphenyl-1-picryl-hydroxyl (86.3%) and FRAP (200 mmol/100 g) were observed in lemongrass leaf tip methanolic extracts. Moreover, lemongrass leaf tip hydro-methanolic extracts portrayed maximum zone of inhibition against E. coli and Staphylococcus aureus i.e. 16.7 and 18.2 mm, respectively. Practical implications This study demonstrated higher antimicrobial and antioxidant activities of the tip of lemongrass leaves as compared with mid and base portions, hence suggesting its role in the improvement of physicochemical, antimicrobial and antioxidant properties of food products. Consequently, the application of lemongrass methanolic extract up to 10% remarkably enhanced the nutritional value and sensorial acceptance of the beverages. Originality/value The present research draws evidence from laboratory analysis of fresh lemongrass grown in Pakistan. The findings suggest that lemongrass methanolic extracts could be used as a nutritionally rich source of antioxidant activity in functional drinks.

scholarly journals Antioxidant and antimicrobial potential of artichoke (Cynara scolymus L.) extract in beef patties

2018 ◽  
Vol 36 (No. 2) ◽  
pp. 154-162 ◽  
Author(s):  
Ergezer Haluk ◽  
Kaya Halil Ibrahim ◽  
Şimşek Ömer
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Coliform Bacteria ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Antimicrobial Potential ◽  
Beef Patties ◽  
Antioxidant And Antimicrobial Activity

The antioxidant and antimicrobial potential of artichoke extract (AE) in raw beef patties (RBPs) was evaluated during the storage. The RBPs were prepared with the addition of 500 and 1000 ppm AE. Also, Escherichia coli ATCC25922 and Listeria monocytogenes ATCC19118 were inoculated to each RBP to follow the antimicrobial activity. An evaluation of the instrumental colour, pH, total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, TBARS and microbiological properties was carried out during storage. The evaluation revealed that the a* value decreased significantly, so that the reduction level of the AE500 and AE1000 samples during storage was  35, 57, and 56%, respectively, in the control. AE addition slightly decreased the pH of RBPs, which varied between 5.72 and 5.96. The TPC and DPPH values of samples with added 500 and 1000 ppm AE were 2 to 3-fold and 3 to 7-fold higher than in the control, respectively. Also, the TBARS values were determined as 43 and 54% lower than in the control at the end of storage when 500 and 1000 ppm AE were used. The AE in RBP inhibited the viability of total aerobic psychrophilic bacteria, coliform bacteria and yeast-mould in a concentration-dependent manner. AE prevented the growth of E. coli ATCC25922 and L. monocytogenes ATCC19118 inoculated to the RBPs. In conclusion, 1000 ppm AE was sufficient for antioxidant and antimicrobial activity in RBP. To our knowledge, this is the first study that presents the antioxidant and antimicrobial activity of AE used in a food model system.

scholarly journals Synthesis and Antimicrobial Activity of Some New Substituted Quinoxalines

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4198 ◽  
Author(s):  
Mohamed A. El-Atawy ◽  
Ezzat A. Hamed ◽  
Mahjoba Alhadi ◽  
Alaa Z. Omar
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Spectral Data ◽  
Elemental Analysis ◽  
Bacterial Species ◽  
Antimicrobial Spectrum ◽  
Fungal Strains ◽  
Nitrogen Nucleophiles

A number of new symmetrically and asymmetrically 2,3-disubstituted quinoxalines were synthesized through functionalization of 2,3-dichloroquinoxaline (2,3-DCQ) with a variety of sulfur and/or nitrogen nucleophiles. The structures of the obtained compounds were established based on their spectral data and elemental analysis. The antimicrobial activity for the prepared compounds was investigated against four bacterial species and two fungal strains. The symmetrically disubstituted quinoxalines 2, 3, 4, and 5 displayed the most significant antibacterial activity, while compounds 6a, 6b, and the pentacyclic compound 10 showed considerable antifungal activity. Furthermore, compounds 3f, 6b showed broad antimicrobial spectrum against most of the tested strains.

Exploitation of the Antioxidant Potential of Geranium Macrorrhizum (Geraniaceae): Hepatoprotective and Antimicrobial Activities

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Niko S. Radulović ◽  
Milan B. Stojković ◽  
Snežana S. Mitić ◽  
Pavle J. Randjelović ◽  
Ivan R. Ilić ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Antioxidant Potential ◽  
Human Pathogens ◽  
Methanol Extracts

In this study we evaluated in vitro (radical scavenging) and in vivo (hepatoprotective effect) antioxidant activities and antimicrobial properties of the extracts of the above- and underground parts of Geranium macrorrhizum L. (Geraniaceae), an ethnopharmacologically renowned plant species. The antioxidant activity and total phenol and flavonoid contents of four different solvent extracts were evaluated by seven different methods. The methanol extracts, administered i.p. to rats (120-480 mg/kg), were evaluated for hepatoprotective activity in a CCl4-induced hepatotoxicity model. The same extracts were tested for antimicrobial activity against seven bacterial and two fungal species. The administered methanol extracts with the highest antioxidant potential showed a significant dose-dependent hepatoprotective action against CCl4-induced liver damage in both decreasing the levels of liver transaminases and bilirubin and in reducing the extent of morphological malformations of the liver. The leaf methanol extract displayed a very strong antibacterial activity, especially against Staphylococcus aureus, with low minimal inhibitory and bactericidal concentrations. These results justify the frequent use of this plant species in folk medicine. Besides the known astringent effect, one can expect that the observed antimicrobial activity against several human pathogens contributes to the wound healing properties of this plant.

scholarly journals Antioxidant and Antimicrobial Activities of Withania frutescens

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601
Author(s):  
Laila El Bouzidi ◽  
Mustapha Larhsini ◽  
Mohamed Markouk ◽  
Abdelaziz Abbad ◽  
Lahcen Hassani ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Anticandidal Activity ◽  
First Time ◽  
Antioxidant And Antimicrobial Activities

In the present study, we report for the first time the antioxidant and antimicrobial activities of Withania frutescens (L.) Pauquy roots and leaves. Total phenolic content was determined using the Folin-Ciocalteu method and antioxidant activity was evaluated by the DPPH free radical scavenging and reducing power methods. Antimicrobial activity tests were carried out against ten bacterial species involved in nosocomial infections and two opportunistic clinical yeast isolates. The ethyl acetate and n-butanol leaf fractions exhibited the highest DPPH radical scavenging activity with IC50 = 4.53 ± 0.12 and 8.49 ± 0.46 μg/mL, respectively. The n-butanol root fraction showed the greatest reducing power comparable with that of quercetin at 0.4 mg/mL. The dichloromethane leaf fraction exhibited the highest antibacterial activity against both Gram-positive and Gram-negative bacteria with MIC values ranging between 50 and 400 μg/mL, depending on the tested bacteria. However, none of the examined extracts exhibited anticandidal activity. The polyphenol and glycowithanolide constituents appeared to be responsible for the antioxidant capacity of W. frutescens, whereas the observed antimicrobial activity may be due to the presence of withanolides.

scholarly journals Gallic acid as the major antioxidant in pequi (Caryocar brasiliense Camb.) fruit peel

2015 ◽  
Vol 17 (4) ◽  
pp. 592-598 ◽  
Author(s):  
L. B. ROCHA ◽  
A. M. MELO ◽  
S. L. A. PAULA ◽  
S. A. M. NOBRE ◽  
I. N. ABREU
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Gallic Acid ◽  
Silica Gel ◽  
Radical Scavenging ◽  
Water Extract ◽  
Free Form ◽  
Attractive Alternative ◽  
Fruit Peel ◽  
Main Compound

ABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products.

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