Identification of Silver Nanoparticles in the Small Intestinal Mucosa, Liver, and Spleen of Rats by Transmission Electron Microscopy

Citrate stabilized silver (Ag) colloidal solution were synthesized and characterized for crystallographic and surface properties by using transmission electron microscopy (TEM) and zeta potential measurement techniques. TEM investigation depicted the size of Ago ranges from 5 to 50 nm with smaller particles having single crystal structure while larger particles with structural defects (such as multiply twinned, high coalescence and Moire patterns). ?-potential measurement confirms the presence of Ag+ in nAg stock solution. The shift in ?-potential measurement by +25.1 mV in the filtered solution suggests the presence of Ag+ in Ago nanoparticles.

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Catalytic Reduction of Nitrobenzene Using Silver Nanoparticles Embedded Calcium Alginate Film

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2019.16669 ◽

2019 ◽

Vol 19 (11) ◽

pp. 7487-7492 ◽

Cited By ~ 5

Author(s):

Supriya ◽

Jayanta Kumar Basu ◽

Sonali Sengupta

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Silver Nanoparticles ◽

Sodium Borohydride ◽

Calcium Alginate ◽

Catalytic Reduction ◽

Average Particle Size ◽

Catalyst Loading ◽

Average Particle ◽

Transmission Electron

Synthesis of silver nanoparticles embedded on calcium alginate film and the catalytic property of this film in the reduction of nitrobenzene with sodium borohydride are demonstrated in this work. Natural polymer alginate acts as effective reducing and stabilizing agent in synthesis of silver nanoparticles. Effect of different parameters on the preparation of silver nanoparticles, such as, temperature, concentration of silver precursor and heating time was investigated. As-prepared silver nanoparticles were characterized by transmission electron microscopy, scanning electron microscopy, UV-Vis spectrometry, and atomic absorption spectrometry. Transmission electron microscopy analysis con-firms the formation of silver nanoparticles with particles size range of 3–19 nm and average particle size was found to be 10±4 nm. Effect of concentration of nitrobenzene and sodium borohydride, catalyst loading and temperature on the catalytic reduction of nitrobenzene was studied. Reusability of catalyst was examined in this reduction reaction and the catalyst shows good activity up to 10th run.

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Properties of Silver Nanoparticles Prepared by the Electric Spark Dispersion Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.872.74 ◽

2013 ◽

Vol 872 ◽

pp. 74-78 ◽

Cited By ~ 3

Author(s):

S.P. Zhuravkov ◽

Evgeny Plotnikov ◽

Dmitry Martemiyanov ◽

Nikolay A. Yavorovsky ◽

Ulrich Hasse ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Silver Nanoparticles ◽

Structural Characteristics ◽

Silver Particles ◽

Dispersion Method ◽

Force Microscopy ◽

Atomic Force ◽

Transmission Electron ◽

Light Scattering Spectroscopy

The morphological and structural characteristics of nanoscale silver particles obtained by the method of electric spark dispersion of metal granules in the liquid aprotic medium were obtained using atomic force microscopy, transmission electron microscopy, and dynamic light scattering spectroscopy. The specific surface, morphology, structure and the distribution by size of the particles are presented.

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The Aggregation Study and Characterization of Silver Nanoparticles

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.263.165 ◽

2017 ◽

Vol 263 ◽

pp. 165-169

Author(s):

Silvia Chowdhury ◽

Faridah Yusof ◽

Nadzril Sulaiman ◽

Mohammad Omer Faruck

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Particle Size ◽

Silver Nanoparticles ◽

Absorption Spectrum ◽

Average Particle Size ◽

Average Particle ◽

Transmission Electron ◽

Vis Spectroscopy

In this article, we have studied the process of silver nanoparticles (AgNPs) aggregation and to stop aggregation 0.3% Polyvinylpyrrolidone (PVP) was used. Aggregation study carried out via UV-vis spectroscopy and it is reported that the absorption spectrum of spherical silver nanoparticles were found a maximum peak at 420 nm wavelength. Furthermore, Transmission Electron Microscopy (TEM) were used to characterized the size and shape of AgNPs, where the average particle size is around 10 to 25 nm in diameter and the AgNPs shape is spherical. Next, Dynamic Light Scattering (DLS) were used, owing to observed size distribution and self-correlation of AgNPs.

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Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Crystals ◽

10.3390/cryst10050395 ◽

2020 ◽

Vol 10 (5) ◽

pp. 395 ◽

Cited By ~ 1

Author(s):

Juan Carlos Martínez Espinosa ◽

Raúl Carrera Cerritos ◽

Maria Antonieta Ramírez Morales ◽

Karla Paola Sánchez Guerrero ◽

Rocio Alejandra Silva Contreras ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Photoelectron Spectroscopy ◽

Bacterial Resistance ◽

Antimicrobial Effect ◽

X Ray ◽

Transmission Electron ◽

Green Route

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.

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Colorimetric Estimation of 3–Mercapto-1, 2-Propanediol by Silver Nanoparticles

Journal of Surface Science and Technology ◽

10.18311/jsst/2016/6601 ◽

2016 ◽

Vol 32 (1-2) ◽

pp. 45

Author(s):

Swarnali Maiti ◽

Gadadhar Barman ◽

Jayasree Konar Laha

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Silver Nanoparticles ◽

Thiol Group ◽

Green Method ◽

Biologically Relevant ◽

Transmission Electron ◽

Visible Spectra ◽

Uv Visible ◽

Self Aggregation

Interaction of 3 -mercapto-1, 2- propanediol (3MPD) (C3H8O2S, 3MPD) with silver nanoparticles prepared by green method has been studied by electronic absorption spectroscopy and transmission electron microscopy. Here we report the changes in properties of AgNP in presence of biologically relevant molecule 3MPD. The assembly and the aggregation of the AgNP are established using UV-Visible spectra and transmission electron microscopy. The biosynthesized AgNP has been used as colorimetric sensor for detection of 3MPD. A new peak generated at 612 nm due to the self aggregation of silver nanoparticles occurred by the interaction of thiol group present in 3 MPD with AgNP. A calibration curve between the absorbance at 612 nm and the concentration of 3 MPD enabled us to estimate 3MPD present in water in presence of SDS.

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Cottonseed Oilcake Extract Mediated Green Synthesis of Silver Nanoparticles and Its Antibacterial and Cytotoxic Activity

Journal of Nanomaterials ◽

10.1155/2016/7412431 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

Muthusamy Govarthanan ◽

Min Cho ◽

Jung-Hee Park ◽

Jum-Suk Jang ◽

Young-Joo Yi ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Silver Nanoparticles ◽

Fourier Transform ◽

Green Synthesis ◽

Pseudomonas Syringae ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron ◽

Xrd Techniques

Agroindustrial byproduct mediated green synthesis of silver nanoparticles was carried out using cottonseed oilcake (CSOC) extract. The aqueous silver nitrate formed stable silver nanoparticles with CSOC extract as a reducing agent for Ag+to Ag0. The synthesized nanoparticles were characterized using energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD) techniques. The synthesized silver nanoparticles (AgNPs) (4 mM) significantly inhibited the growth of phytopathogens,Pseudomonas syringaepv.actinidiaeandRalstonia solanacearum. Further, cytotoxicity of AgNPs was evaluated using rat splenocyte cells. The splenocyte viability was decreased according to the increasing concentration of AgNPs and 90% of cell death was observed at 100 μg/mL.

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One-step synthesis of gold and silver non-spherical nanoparticles mediated by Eosin Methylene Blue agar

Scientific Reports ◽

10.1038/s41598-019-55744-0 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Diego Alberto Lomelí-Rosales ◽

Adalberto Zamudio-Ojeda ◽

Sara Angélica Cortes-Llamas ◽

Gilberto Velázquez-Juárez

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Methylene Blue ◽

Silver Nanoparticles ◽

Metal Nanoparticles ◽

Reduction Process ◽

Gold And Silver Nanoparticles ◽

Transmission Electron ◽

One Step ◽

Conventional Plate

AbstractNowadays, there are several approaches reported to accomplish the green synthesis of metal nanoparticles by using bacterial and fungi supernatants or by-products generated by these microorganisms. Therefore, agars as solely reductive regents have started to be used in order to obtain metal nanoparticles. This paper shows the results of the synthesis of gold and silver nanoparticles with different morphology, mainly triangular and truncated triangular, using Eosin Methylene Blue (EMB) agar as reducing agent. To control the reaction process, the necessary activation energy for the reducer was provided by three different techniques: microwave radiation, using a domestic microwave oven, ultraviolet radiation, and heating on a conventional plate. The evolution of the reduction process and stability of the samples was performed by ultraviolet visible spectroscopy. Morphology was carefully analyzed using high-resolution transmission electron microscopy (HRTEM) and Transmission electron microscopy (TEM). A one step synthesis for gold and silver nanoparticles was optimized with an eco-friendly and economic process.

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