Selective inhibitory potential of silver nanoparticles on the harmful cyanobacterium Microcystis aeruginosa

Facts over microorganisms to predominate periodontitis, shifting of human microbiota by Dimocarpus longan (D. longan) plant, and potentiation of antimicrobial activity by biosynthetic silver nanoparticles (SNPs) intended present study to biosynthesize, optimize, characterize and evaluate the antimicrobial potential of silver nanoparticles (SNPs) obtained using D. longan leaves aqueous extract (DLLAE). Study involved preparation of DLLAE using decoction method. The DLLAE was subjected to biosynthesis of SNPs followed by optimization (using UV-Visible spectrometry), characterization (by FTIR, FESEM, XRD, and EDX), stability, and antimicrobial activity of SNPs against periodontitis triggering human microflora. Biosynthesized SNPs exhibited signal between 416-453 nm. Optimization study established AgNO3 concentration (5 mM), pH 4, DLLAE and AgNO3 ratio (1:9) and temperature (60°C) as parametric requirement for SNPs biosynthesis using DLLAE. Stability study exhibited signal between 489-553 nm supporting SNPs stability. Characterization data of FESEM showed that SNPs were poly dispersed, and spherical shaped. Biosynthesized SNPs size ranged from 74.82 nm to 131.5 nm. The XRD data revealed presence of signals at 38.08°, 44.33°, 64.47°, and 78.83° 2θ values indexed to silver cubic structure planes. In EDX study, silver exhibited strong signal (55.54%). Antimicrobial investigation explored the high inhibitory potential of SNPs against B. subtilis and P. aeruginosa; and low inhibitory potential against S. aureus and E. coli. Present study conclude that biosynthesis of SNPs using DLLAE is an efficient method and biosynthetic SNPs possess high antimicrobial potential against P. aeruginosa and B. subtilis the periodontitis triggering pathogens.

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EFFECT OF SILVER NANOPARTICLES IN EXPERIMENTAL PERITONITIS

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2015-2-67-74 ◽

2015 ◽

Vol 14 (2) ◽

pp. 67-74

Author(s):

D. V. Chegodar ◽

A. V. Kubyshkin ◽

V. V. Panasenko

Keyword(s):

Silver Nanoparticles ◽

Blood Serum ◽

Proteinase Inhibitor ◽

Inflammatory Process ◽

Local Level ◽

Experimental Peritonitis ◽

Fecal Suspension ◽

Inhibitory Potential ◽

Antiinflammatory Effects ◽

20 Nm

In our research we have investigated the effect of intraperitoneal introduction of silver nanoparticles solution (linear particle size of 10–20 nm) into intact animals and in the simulation of experimental peritonitis. We have evaluated the indices of nonspecific proteinases and their inhibitors in blood serum and peritoneum lavage. The intraperitoneal introduction of silver nanoparticles solution to intact animals leads to the minimal reaction of proteinase-inhibitor systems components in blood serum and peritoneum lavage. When modeling inflammatory process in peritoneum cavity by intraperitoneal injection of 10% filtered fecal suspension of rats, application of silver nanoparticles solution is accompanied by decrease in extent of activation of proteinases and preservation of inhibitory potential, both at systemic and local level, which can indicate antiinflammatory effects of nanosilver.

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Anti-algae efficacy of silver nanoparticles to Microcystis aeruginosa : Influence of NOM, divalent cations, and pH

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2016.09.009 ◽

2016 ◽

Vol 509 ◽

pp. 492-503 ◽

Cited By ~ 19

Author(s):

Tiandi Huang ◽

Minghao Sui ◽

Xin Yan ◽

Xuan Zhang ◽

Zhen Yuan

Keyword(s):

Silver Nanoparticles ◽

Microcystis Aeruginosa ◽

Divalent Cations

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Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Scientific Reports ◽

10.1038/s41598-021-92812-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Bartosz Skóra ◽

Urszula Krajewska ◽

Anna Nowak ◽

Andrzej Dziedzic ◽

Adriana Barylyak ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Biofilm Formation ◽

Metallic Nanoparticles ◽

Antibacterial Properties ◽

Hospital Treatment ◽

Deep Penetration ◽

Bacteria Growth ◽

Adhesion Ability ◽

Inhibitory Potential

AbstractDrug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.

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