Green synthesis of silver nanoparticles using Carum copticum: Assessment of its quorum sensing and biofilm inhibitory potential against gram negative bacterial pathogens

2020 ◽  
Vol 144 ◽  
pp. 104172 ◽  
Author(s):  
Faizan Abul Qais ◽  
Anam Shafiq ◽  
Iqbal Ahmad ◽  
Fohad Mabood Husain ◽  
Rais Ahmad Khan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Quorum Sensing ◽  
Green Synthesis ◽  
Bacterial Pathogens ◽  
Gram Negative ◽  
Carum Copticum ◽  
Inhibitory Potential

Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens

2012 ◽  
Vol 45 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Issac Abraham Sybiya Vasantha Packiavathy ◽  
Palani Agilandeswari ◽  
Khadar Syed Musthafa ◽  
Shunmugiah Karutha Pandian ◽  
Arumugam Veera Ravi
Keyword(s):  
Quorum Sensing ◽  
Secondary Metabolite ◽  
Bacterial Pathogens ◽  
Methyl Eugenol ◽  
Gram Negative ◽  
Cuminum Cyminum ◽  
Inhibitory Potential

Synergistic Antibacterial Efficacy of Biogenic Synthesized Silver Nanoparticles using Ajuga bractosa with Standard Antibiotics: A Study Against Bacterial Pathogens

2020 ◽  
Vol 21 (3) ◽  
pp. 206-218 ◽  
Author(s):  
Sadia Nazer ◽  
Saiqa Andleeb ◽  
Shaukat Ali ◽  
Nazia Gulzar ◽  
Tariq Iqbal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bacterial Pathogens ◽  
Potential Effect ◽  
Innovative Technology ◽  
Multi Drug Resistance ◽  
Phytochemical Screening ◽  
Cell Proliferation Inhibition ◽  
Agar Well Diffusion Assay ◽  
Scanning Electron

Background: Multi-drug resistance in bacterial pathogens is a major concern of today. Green synthesis technology is being used to cure infectious diseases. Objectives: The aim of the current research was to analyze the antibacterial, antioxidant, and phytochemical screening of green synthesized silver nanoparticles using Ajuga bracteosa. Methods: Extract of A. bracteosa was prepared by maceration technique. Silver nanoparticles were synthesized using A. bracteosa extract and were confirmed by UV-Vis spectrophotometer, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, TLC-Bioautography, TLC-Spot screening, antioxidant, and phytochemical screening were also investigated. Results: UV-Vis spectrum and Scanning electron microscopy confirmed the synthesis of green nanoparticles at 400 nm with tube-like structures. FTIR spectrum showed that functional groups of nanoparticles have a role in capping and stability of AgNP. Agar well diffusion assay represented the maximum antibacterial effect of ABAgNPs against Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa at 0.10 g/mL concentration compared to ABaqu. Two types of interactions among nanoparticles, aqueous extract, and antibiotics (Synergistic and additive) were recorded against tested pathogens. Crystal violet, MTT, TLC-bio-autography, and spot screening supported the findings of the antibacterial assay. Highest antioxidant potential effect in ABaqu was 14.62% (DPPH) and 13.64% (ABTS) while 4.85% (DPPH) and 4.86% (ABTS) was recorded in ABAgNPs. Presence of phytochemical constituents showed pharmacological importance. Conclusion: It was concluded that green synthesis is an innovative technology in which natural products are conjugated with metallic particles and are used against infectious pathogens. The current research showed the significant use of green nanoparticles against etiological agents.

scholarly journals Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Royal Jelly ◽  
Fluorescence Emission ◽  
Spherical Particles ◽  
Ag Nps ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

scholarly journals Green Synthesis of Silver Nanoparticles Using Novel Launaea Procumbens Leaves Extract and Screening Its Antibacterial Activity

2021 ◽  
Author(s):  
Sanjay Ratan Kumavat ◽  
SATYENDRA MISHRA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Launaea Procumbens

Abstract Plants are emerging as a cost-effective and ecofriendly method for green synthesis of nanoparticles. The plant extract Launaea procumbens was used as a reduction agent in the green synthesis of silver nanoparticles. UV-Visible spectroscopy, HR-TEM, SAED, FE-SEM, EDAX, DLS, and FT-IR were used to study the green synthesized silver nanoparticles. UV-Vis spectroscopy of a prepared silver solution revealed maximum absorption at 435 nm. The synthesized silver nanoparticles were found to be spherical in shape with a size in the range of 24.28 to 31.54 nm. DLS analysis was used to determine the size of the green synthesized silver nanoparticles, which showed outstanding antibacterial action against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, as well as Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Gram-positive Bacillus subtilis had a maximum zone of inhibition of 20 mm, Staphylococcus aureus had a zone of inhibition of 19 mm, and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa had zones of inhibition of 13 mm.

The role of biosynthesized silver nanoparticles in antimicrobial mechanisms

2021 ◽  
Vol 22 ◽  
Author(s):  
Bianca PizzornoBackx ◽  
Mayara Santana dos Santos ◽  
Otávio Augusto Leitão dos Santos ◽  
Sérgio Antunes Filho
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antimicrobial Properties ◽  
New Drugs ◽  
Gram Negative Bacteria ◽  
New Materials ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Emergence Of Resistance

: Nanotechnology is an area of science able to develop new materials. The relation between nanotechnology and microbiology is essential for the development of new drugs and vaccines. The main advantage of blend in both areas is to associate the latest technology to obtain new ways to solve problems related to microorganisms. This review seeks to investigate nanoparticle formation's antimicrobial properties, primarily when connected to the green synthesis of silver nanoparticles. The development of new sustainable methods for nanoparticle production has been instrumental in designing alternative, non-toxic, energy-friendly, and environmentally friendly routes. In this sense, it is necessary to study silver nanoparticles' green synthesis concerning their antimicrobial properties. Antimicrobial mechanisms of silver nanoparticles demonstrate efficiency to gram-positive bacteria, gram-negative bacteria, fungi, viruses, and parasites. However, attention is needed with the emergence of resistance to these antimicrobials. This article seeks to relate the parameters of green silver-based nanosystems with the efficiency of antimicrobial activity.

Green Synthesis of Silver Nanoparticles using the Leaves Extract of Filicium decipiens and its Anti- Microbial Activity

2021 ◽  
Vol 10 (3) ◽  
pp. 16-24
Author(s):  
Sherin Monichan ◽  
P. Mosae Selvakumar ◽  
Christine Thevamithra ◽  
M. S. A. Muthukumar Nadar ◽  
Jesse Joel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Average Diameter ◽  
Diffusion Method ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Gram Negative ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Almost All

Silver nanoparticles has been used since ages, even till now it is exploited in almost all areas like medicine, textiles, industries, cosmetics, purification, dying and many more. There are many approaches which are used to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or very costly. Therefore, green synthesis of silver nanoparticles (AgNPs) using leaves of Filicium decipiens eco-friendly and a very reliable method to procure AgNPs. Characterization of synthesized AgNPs were then done using UV-Vis spectroscopy and fluorescence which confirmed the formation of AgNPs, scanning electron microscope (SEM)confirmed its shape to be round and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative (Escherichia. Coli) and gram positive (Bacillus.spc) were done using well-diffusion method and also its application of antimicrobial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed more efficiency in gram negative bacteria than gram- positive.

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