Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola

2014 ◽  
Vol 37 (1) ◽  
pp. 153-159 ◽  
Author(s):  
Wen Du ◽  
Jiandong Liang ◽  
Yanfeng Han ◽  
Jianping Yu ◽  
Zongqi Liang
Keyword(s):  
Nitric Oxide ◽  
Fungal Elicitor ◽  
Submerged Cultures ◽  
Shiraia Bambusicola

scholarly journals Nitric oxide mediates the fungal-elicitor-enhanced biosynthesis of antioxidant polyphenols in submerged cultures of Inonotus obliquus

Microbiology ◽  
2009 ◽  
Vol 155 (10) ◽  
pp. 3440-3448 ◽  
Author(s):  
Weifa Zheng ◽  
Kangjie Miao ◽  
Yanxia Zhang ◽  
Shenyuan Pan ◽  
Meimei Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Higher Plants ◽  
Antioxidant Activities ◽  
Fungal Elicitor ◽  
Submerged Cultures ◽  
No Donor ◽  
Inonotus Obliquus ◽  
Pal Activity ◽  
Exogenous Addition ◽  
Nos Inhibitor

A fungal elicitor prepared from the cell debris of the plant-pathogenic ascomycete Alternaria alternata induces multiple responses by Inonotus obliquus cells, including an increase in generation of nitric oxide (NO), activity of phenylalanine ammonia lyase (PAL) and accumulation of total mycelial phenolic compounds (TMP), but does not trigger production of oxylipins or jasmonic acid (JA). The role of NO in TMP production was investigated via the effects of the NO-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPITO) and the nitric oxide synthase (NOS) inhibitor aminoguanidine (AG). TMP profiles were assayed using 1H NMR spectroscopy combining multivariate pattern recognition strategies. Pretreatment of I. obliquus mycelia with cPITO or AG suppressed not only elicitor-enhanced NO generation and PAL activity, but also the elicitor-induced increase in TMP production. This TMP reduction by either a NO scavenger or a NOS inhibitor was reversed by exogenous addition of either a NO donor, sodium nitroprusside, or JA separately. NMR-based metabonomic analysis of TMP profiles showed that the induced TMP were hispidin analogues including inoscavins, phelligridins, davallialactone and methyldavallialactone, which possess high antioxidant activities. Thus, NO mediates an elicitor-induced increase in production of antioxidant polyphenols in I. obliquus via a signalling pathway independent of oxylipins or JA, a mechanism which differs from those in some higher plants.

scholarly journals Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ning Zhao ◽  
Yingying Yu ◽  
Yunxia Yue ◽  
Mingzhu Dou ◽  
Bingjing Guo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Nitric Oxide Donor ◽  
Transcriptional Level ◽  
Active Metabolites ◽  
Signal Molecule ◽  
Regulatory Pathways ◽  
Shiraia Bambusicola ◽  
Hypocrellin A ◽  
Exogenous Addition

AbstractShiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.

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