Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola

A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of hypocrellin A, hypocrellin B from Fermentation Broth of Shiraia bambusicola LPHP-89. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase consisted of methanol、water and acetic acid (60:40:1, v/v/v) and was pumped at a flow rate of 1.0 mL/min. The injection volume was5 μL. The quantitation wavelength was set at 254 nm.Total run time was 20 min and retention times for hypocrellin A and hypocrellin B were 10.06 and 15.38 min, respectively.

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Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Fermentation ◽

10.3390/fermentation7040316 ◽

2021 ◽

Vol 7 (4) ◽

pp. 316

Author(s):

Xiaoxiao Yan ◽

Yongdi Wen ◽

Menghua Hu ◽

Zhenqiang Wu ◽

Xiaofei Tian

Keyword(s):

Carbon Source ◽

Submerged Fermentation ◽

Microscope Observation ◽

Time Increase ◽

Anthracene Derivative ◽

Shiraia Bambusicola ◽

Hypocrellin A ◽

Cultivation Strategy ◽

Fermentation Media

Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.

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Cluster analysis on hypocrellin A content in anamorphsof Shiraia bambusicola

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20100805 ◽

2010 ◽

Vol 35 (8) ◽

Cited By ~ 1

Author(s):

DU Wen

Keyword(s):

Cluster Analysis ◽

Shiraia Bambusicola ◽

Hypocrellin A

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Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola

Biotechnology Letters ◽

10.1007/s10529-014-1665-4 ◽

2014 ◽

Vol 37 (1) ◽

pp. 153-159 ◽

Cited By ~ 14

Author(s):

Wen Du ◽

Jiandong Liang ◽

Yanfeng Han ◽

Jianping Yu ◽

Zongqi Liang

Keyword(s):

Nitric Oxide ◽

Fungal Elicitor ◽

Submerged Cultures ◽

Shiraia Bambusicola

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De Novo Transcriptome Assembly in Shiraia bambusicola to Investigate Putative Genes Involved in the Biosynthesis of Hypocrellin A

International Journal of Molecular Sciences ◽

10.3390/ijms17030311 ◽

2016 ◽

Vol 17 (3) ◽

pp. 311 ◽

Cited By ~ 25

Author(s):

Ning Zhao ◽

Xi Lin ◽

Shan-Shan Qi ◽

Zhi-Mei Luo ◽

Shuang-Lin Chen ◽

...

Keyword(s):

De Novo ◽

Transcriptome Assembly ◽

De Novo Transcriptome Assembly ◽

De Novo Transcriptome ◽

Shiraia Bambusicola ◽

Hypocrellin A

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Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent TolerantBacillus tequilensisRG-01: Thermostable and Surfactant Resistant

The Scientific World JOURNAL ◽

10.1155/2014/972763 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 14

Author(s):

Soni Tiwari ◽

Neha Shukla ◽

Pooja Mishra ◽

Rajeeva Gaur

Keyword(s):

Tween 80 ◽

Inhibitory Effect ◽

Industrial Applications ◽

Bacterial Strains ◽

Enhanced Production ◽

Bacillus Tequilensis ◽

Amylase Production ◽

Tween 40 ◽

Tween 60 ◽

Triton X

Ten bacterial strains isolated from the soil samples in the presence of cyclohexane were screened for amylase production. Among them, culture RG-01 was adjudged as the best amylase producer and was identified asBacillus tequilensisfrom MTCC, Chandigarh. The isolate showed maximum amylase production (8100 U/mL) in the presence of starch, peptone, and Ca2+ions at 55°C pH 7.0 within 24 h of incubation. The enzyme was stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively. The presence of benzene, methanol, and ethanol marginally reduced the amylase stability, respectively. The enzyme was showed it 100% activity at 55°C and pH 7.0 with 119% and 127% stability at 55°C and pH 7.0, respectively. The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. Only Triton-X-100 showed a moderate inhibitory effect (5%) on amylase activity. This isolate (Bacillus tequilensisRG-01) may be useful in several industrial applications owing to its thermotolerant and organic solvents and surfactants resistance characteristics.

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