Enhanced production of hypocrellin A by ultrasound stimulation in submerged cultures of Shiraia bambusicola

2017 ◽  
Vol 38 ◽  
pp. 214-224 ◽  
Author(s):  
Chun Xiao Sun ◽  
Yan Jun Ma ◽  
Jian Wen Wang
Keyword(s):  
Submerged Cultures ◽  
Shiraia Bambusicola ◽  
Ultrasound Stimulation ◽  
Enhanced Production ◽  
Hypocrellin A

Gentic overexpression increases production of hypocrellin A in Shiraia bambusicola S4201

2019 ◽  
Vol 57 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Dan Li ◽  
Ning Zhao ◽  
Bing-Jing Guo ◽  
Xi Lin ◽  
Shuang-Lin Chen ◽  
...  
Keyword(s):  
Shiraia Bambusicola ◽  
Hypocrellin A

A HPLC-UV Method for Simultaneous Determination of Hypocrellin A and B from Fermentation Broth of Shiraia Bambusicola LPHP-89

2015 ◽  
Vol 1092-1093 ◽  
pp. 662-665
Author(s):  
Sai Dan Zhang ◽  
Ping Lv ◽  
Xiu Zhu
Keyword(s):  
High Performance ◽  
Fermentation Broth ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Shiraia Bambusicola ◽  
Rp Hplc ◽  
Hypocrellin A ◽  
Hypocrellin B ◽  
Liquid Chromatographic

A rapid, sensitive and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous quantification of hypocrellin A, hypocrellin B from Fermentation Broth of Shiraia bambusicola LPHP-89. Isocratic RP-HPLC system with C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) and a detector UV-VWD was employed. The mobile phase consisted of methanol、water and acetic acid (60:40:1, v/v/v) and was pumped at a flow rate of 1.0 mL/min. The injection volume was5 μL. The quantitation wavelength was set at 254 nm.Total run time was 20 min and retention times for hypocrellin A and hypocrellin B were 10.06 and 15.38 min, respectively.

scholarly journals Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 316
Author(s):  
Xiaoxiao Yan ◽  
Yongdi Wen ◽  
Menghua Hu ◽  
Zhenqiang Wu ◽  
Xiaofei Tian
Keyword(s):  
Carbon Source ◽  
Submerged Fermentation ◽  
Microscope Observation ◽  
Time Increase ◽  
Anthracene Derivative ◽  
Shiraia Bambusicola ◽  
Hypocrellin A ◽  
Cultivation Strategy ◽  
Fermentation Media

Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.

Enhanced production of fumigaclavine C by ultrasound stimulation in a two-stage culture of Aspergillus fumigatus CY018

2014 ◽  
Vol 159 ◽  
pp. 112-117 ◽  
Author(s):  
Ling-Yun Yao ◽  
Yi-Xiang Zhu ◽  
Rui-Hua Jiao ◽  
Yan-Hua Lu ◽  
Ren-Xiang Tan
Keyword(s):  
Aspergillus Fumigatus ◽  
Two Stage ◽  
Ultrasound Stimulation ◽  
Enhanced Production ◽  
Two Stage Culture

Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola

2014 ◽  
Vol 37 (1) ◽  
pp. 153-159 ◽  
Author(s):  
Wen Du ◽  
Jiandong Liang ◽  
Yanfeng Han ◽  
Jianping Yu ◽  
Zongqi Liang
Keyword(s):  
Nitric Oxide ◽  
Fungal Elicitor ◽  
Submerged Cultures ◽  
Shiraia Bambusicola
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