Acibenzolar-S-methyl induced resistance to Phytophthora capsici in pepper leaves

2005 ◽  
Vol 49 (4) ◽  
pp. 599-604 ◽  
Author(s):  
O. Baysal ◽  
C. Turgut ◽  
G. Mao
Keyword(s):  
Induced Resistance ◽  
Phytophthora Capsici ◽  
Pepper Leaves

scholarly journals Isolation, Partial Sequencing, and Expression of Pathogenesis-Related cDNA Genes from Pepper Leaves Infected by Xanthomonas campestris pv. vesicatoria

2000 ◽  
Vol 13 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Ho Won Jung ◽  
Byung Kook Hwang
Keyword(s):  
Xanthomonas Campestris ◽  
Lipid Transfer Protein ◽  
Phytophthora Capsici ◽  
Cdna Clones ◽  
Avirulent Strain ◽  
Lipid Transfer ◽  
Genes Encoding ◽  
Abiotic Elicitor ◽  
Pepper Leaves ◽  
Sequencing And Expression

Specific cDNAs showing differential expression in bacteria-infected pepper leaves as opposed to healthy leaves were isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with an avirulent strain of Xanthomonas campestris pv. vesicatoria. Among a total of 282 cDNA clones tested, 36 individual cDNA genes (13%) hybridized strongly or differentially to the cDNA probes from bacteria-infected leaves. Ten Capsicum annuum-induced (CAI) genes encoding putative thionin, lipid transfer protein I and II, osmotin (PR-5), class I chitinase, β-1,3-glucanase, SAR 8.2, stellacyanin, leucine-rich repeat protein, and auxin-repressed protein were identified. Two CAI genes showed little or no sequence homology to the previously sequenced plant genes. Transcripts of the CAI genes were strongly or preferentially induced in pepper tissues by infection with X. campestris pv. vesicatoria or Phytophthora capsici, and by abiotic elicitor treatment. In particular, most of the CAI genes were strongly induced in pepper tissues by ethephon and methyl jasmonate.

scholarly journals Biocontrol Activity and Induction of Systemic Resistance in Pepper by Compost Water Extracts Against Phytophthora capsici

2010 ◽  
Vol 100 (8) ◽  
pp. 774-783 ◽  
Author(s):  
Mee Kyung Sang ◽  
Jeong-Gyu Kim ◽  
Ki Deok Kim
Keyword(s):  
Pseudomonas Syringae ◽  
Fungal Pathogens ◽  
Xanthomonas Campestris ◽  
Disease Incidence ◽  
Phytophthora Capsici ◽  
Systemic Resistance ◽  
Water Extracts ◽  
Pepper Leaves ◽  
Plant Assays ◽  
Pepper Plants

We investigated the effects of water extracts of composts (CWE) from commercial compost facilities for controlling root and foliar infection of pepper plants by Phytophthora capsici. Among 47 CWE tested, CWE from composts Iljuk-3, Iljuk-7, Shinong-8, and Shinong-9 significantly (P < 0.05) inhibited zoospore germination, germ tube elongation, mycelial growth, and population of P. capsici. All selected CWE significantly (P < 0.05) reduced the disease incidence and severity in the seedling and plant assays compared with the controls. However, there were no significant differences in zoospore germination, disease incidence, and disease severity between treatments of untreated, autoclaved, and filtered CWE. In addition, CWE significantly (P < 0.05) suppressed leaf infection of P. capsici through induced systemic resistance (ISR) in plants root-drenched with CWE. The tested CWE enhanced the expression of the pathogenesis-related genes, CABPR1, CABGLU, CAChi2, CaPR-4, CAPO1, or CaPR-10 as well as β-1,3-glucanase, chitinase, and peroxidase activities, which resulted in enhanced plant defense against P. capsici in pepper plants. Moreover, the CWE enhanced the chemical and structural defenses of the plants, including H2O2 generation in the leaves and lignin accumulation in the stems. The CWE could also suppress other fungal pathogens (Colletotrichum coccodes in pepper leaves and C. orbiculare in cucumber leaves) through ISR; however, it failed to inhibit other bacterial pathogens (Xanthomonas campestris pv. vesicatoria in pepper leaves and Pseudomonas syringae pv. lachrymans in cucumber leaves). These results suggest that a heat-stable chemical(s) in the CWE can suppress root and foliar infection by P. capsici in pepper plants. In addition, these suppressions might result from direct inhibition of development and population of P. capsici for root infection, as well as indirect inhibition of foliar infection through ISR with broad-spectrum protection.

Phosphite-induced reactive oxygen species production and ethylene and ABA biosynthesis, mediate the control of Phytophthora capsici in pepper (Capsicum annuum)

2016 ◽  
Vol 43 (6) ◽  
pp. 563 ◽  
Author(s):  
Peiqing Liu ◽  
Benjin Li ◽  
Ming Lin ◽  
Guoliang Chen ◽  
Xueling Ding ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Capsicum Annuum ◽  
Induced Resistance ◽  
Mycelial Growth ◽  
Phytophthora Capsici ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antibiotic Effect ◽  
Pre Treatment ◽  
Aba Biosynthesis

Phytophthora capsici is an oomycete pathogen with a broad host range that inflicts significant damage in vegetables. Phosphite (Phi) is used to control oomycete diseases, but the molecular mechanisms underlying Phi-induced resistance to P. capsici are unknown. Thus, Phi-inhibited mycelial growth on strain LT1534 and primed host defence were analysed. We demonstrated that Phi (>5 µg mL–1) had a direct antibiotic effect on mycelial growth and zoospore production, and that mortality and DNA content were significantly reduced by pre-treatment with Phi. In addition, elevated hydrogen peroxide (H2O2) promoted callose deposition and increased the levels of soluble proteins and Capsicum annuum L. pathogenesis-related 1 (CaPR1) expression. Furthermore, Phi (1 g L–1) significantly increased the transcription of the antioxidant enzyme genes, and the genes involved in ethylene (ET) and abscisic acid (ABA) biosynthesis, as well as mitogen-activated protein kinase (MAPK) cascades. However, pre-treatment with reactive oxygen species (ROS), ABA and ET biosynthesis inhibitors decreased Phi-induced resistance and reduced the expression of ABA-responsive 1 (CaABR1) and lipoxygenase 1 (CaLOX1). In addition, the decreased ROS and ABA inhibited Phi-induced expression of CaMPK17–1. We propose that Phi-induced ROS production, ET and ABA biosynthesis mediate the control of P. capsici, and that ABA functions through CaMPK17–1-mediated MAPK signalling.

scholarly journals Evaluation of tolerance to Phytophthora capsici-the causal agent of foot rot disease in black pepper

2019 ◽  
Vol 18 (01) ◽  
pp. 26-34
Author(s):  
Anh T. Ton
Keyword(s):  
Phytophthora Capsici ◽  
Black Pepper ◽  
Disease Index ◽  
Significant Decline ◽  
Foot Rot ◽  
Greenhouse Condition ◽  
Resistance Level ◽  
Pepper Leaves ◽  
Rot Disease

Vietnam is the leading black pepper export country in the world. However, the production of pepper may be affected by natural disasters, pests such as Phytophthora capsici. The Phytophthora capsica disease has caused a significant decline in pepper yields. The disease is characterized with a high mortality rate (up to 100%) and rapid outbreak and thus is very challenging to control. In the present study, eight strains of P. capsici were isolated from 100 samples of leaf, stem, root and soil that were collected from pepper-growing areas of Dong Nai, Binh Duong and Ba Ria - Vung Tau provinces. These isolates' straits had capacity to cause foot rot on pepper leaf after two days of inoculation. Among them, the isolates with the highest pathogenicity are BR-L1, DN-D1 and DN-D2. In order to determine the resistance level to P. capsici of commonly grown pepper varieties (Vinh Linh, Se, Xanh, Trau and Kuching), the pepper leaves were in vitro infected with P. capsici BR-L1 spores in laboratory and greenhouse conditions. Trau variety showed the highest resistance level to P. capsici with the as indicated by (P < 0.01) disease ratio (74.1%) and disease index (73.7%) after 6 days in laboratory condition and the corresponding numbers of 17.3% and 15.8% after 12 days in greenhouse condition.

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