Phosphite-induced reactive oxygen species production and ethylene and ABA biosynthesis, mediate the control of Phytophthora capsici in pepper (Capsicum annuum)

2016 ◽  
Vol 43 (6) ◽  
pp. 563 ◽  
Author(s):  
Peiqing Liu ◽  
Benjin Li ◽  
Ming Lin ◽  
Guoliang Chen ◽  
Xueling Ding ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Capsicum Annuum ◽  
Induced Resistance ◽  
Mycelial Growth ◽  
Phytophthora Capsici ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antibiotic Effect ◽  
Pre Treatment ◽  
Aba Biosynthesis

Phytophthora capsici is an oomycete pathogen with a broad host range that inflicts significant damage in vegetables. Phosphite (Phi) is used to control oomycete diseases, but the molecular mechanisms underlying Phi-induced resistance to P. capsici are unknown. Thus, Phi-inhibited mycelial growth on strain LT1534 and primed host defence were analysed. We demonstrated that Phi (>5 µg mL–1) had a direct antibiotic effect on mycelial growth and zoospore production, and that mortality and DNA content were significantly reduced by pre-treatment with Phi. In addition, elevated hydrogen peroxide (H2O2) promoted callose deposition and increased the levels of soluble proteins and Capsicum annuum L. pathogenesis-related 1 (CaPR1) expression. Furthermore, Phi (1 g L–1) significantly increased the transcription of the antioxidant enzyme genes, and the genes involved in ethylene (ET) and abscisic acid (ABA) biosynthesis, as well as mitogen-activated protein kinase (MAPK) cascades. However, pre-treatment with reactive oxygen species (ROS), ABA and ET biosynthesis inhibitors decreased Phi-induced resistance and reduced the expression of ABA-responsive 1 (CaABR1) and lipoxygenase 1 (CaLOX1). In addition, the decreased ROS and ABA inhibited Phi-induced expression of CaMPK17–1. We propose that Phi-induced ROS production, ET and ABA biosynthesis mediate the control of P. capsici, and that ABA functions through CaMPK17–1-mediated MAPK signalling.

scholarly journals Doxorubicin activates nuclear factor of activated T-lymphocytes and Fas ligand transcription: role of mitochondrial reactive oxygen species and calcium

2005 ◽  
Vol 389 (2) ◽  
pp. 527-539 ◽  
Author(s):  
Shasi V. Kalivendi ◽  
Eugene A. Konorev ◽  
Sonya Cunningham ◽  
Sravan K. Vanamala ◽  
Eugene H. Kaji ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Lymphocytes ◽  
Fas Ligand ◽  
Reactive Oxygen ◽  
Nuclear Factor ◽  
Oxygen Species ◽  
Rat Cardiomyocytes ◽  
Activated T Lymphocytes ◽  
Pre Treatment ◽  
Fas L

Doxorubicin (DOX), a widely used antitumour drug, causes dose-dependent cardiotoxicity. Cardiac mitochondria represent a critical target organelle of toxicity during DOX chemotherapy. Proposed mechanisms include generation of ROS (reactive oxygen species) and disturbances in mitochondrial calcium homoeostasis. In the present study, we probed the mechanistic link between mitochondrial ROS and calcium in the embryonic rat heart-derived H9c2 cell line and in adult rat cardiomyocytes. The results show that DOX stimulates calcium/calcineurin-dependent activation of the transcription factor NFAT (nuclear factor of activated T-lymphocytes). Pre-treatment of cells with an intracellular calcium chelator abrogated DOX-induced nuclear NFAT translocation, Fas L (Fas ligand) expression and caspase activation, as did pre-treatment of cells with a mitochondria-targeted antioxidant, Mito-Q (a mitochondria-targeted antioxidant consisting of a mixture of mitoquinol and mitoquinone), or with adenoviral-over-expressed antioxidant enzymes. Treatment with GPx-1 (glutathione peroxidase 1), MnSOD (manganese superoxide dismutase) or a peptide inhibitor of NFAT also inhibited DOX-induced nuclear NFAT translocation. Pre-treatment of cells with a Fas L neutralizing antibody abrogated DOX-induced caspase-8- and -3-like activities during the initial stages of apoptosis. We conclude that mitochondria-derived ROS and calcium play a key role in stimulating DOX-induced ‘intrinsic and extrinsic forms’ of apoptosis in cardiac cells with Fas L expression via the NFAT signalling mechanism. Implications of ROS- and calcium-dependent NFAT signalling in DOX-induced apoptosis are discussed.

Aspirin-triggered lipoxin A4 blocks reactive oxygen species generation in endothelial cells: A novel antioxidative mechanism

2007 ◽  
Vol 97 (01) ◽  
pp. 88-98 ◽  
Author(s):  
Christina Barja-Fidalgo ◽  
Vany Nascimento-Silva ◽  
Maria Arruda ◽  
Iolanda Fierro
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Cardiovascular Diseases ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Protective Role ◽  
Ros Generation ◽  
Oxygen Species ◽  
Lipoxin A4 ◽  
Pre Treatment

SummaryLipoxins and their aspirin-triggered carbon-15 epimers have emerged as mediators of key events in endogenous anti-inflammation and resolution. However, the implication of these novel lipid mediators on cardiovascular diseases such as hypertension, atherosclerosis, and heart failure has not been investigated. One of the major features shared by these pathological conditions is the increased production of reactive oxygen species (ROS) generated by vascular NAD(P)H oxidase activation. In this study, we have examined whether an aspirin-triggered lipoxin A4 analog (ATL-1) modulates ROS generation in endothelial cells (EC). Pre-treatment of EC with ATL-1 (1–100 nM) completely blocked ROS production triggered by different agents, as assessed by dihydrorhodamine 123 and hydroethidine. Furthermore, ATL-1 inhibited the phosphorylation and translocation of the cytosplamic NAD(P)H oxidase subunit p47phox to the cell membrane as well as NAD(P)H oxidase activity. Western blot and immunofluorescence microscopy analyses showed that ATL-1 (100 nM) impaired the redox-sensitive activation of the transcriptional factor NF-κB, a critical step in several events associated to vascular pathologies. These results demonstrate that ATL-1 suppresses NAD(P)H oxidase-mediated ROS generation in EC, strongly indicating that lipoxins may play a protective role against the development and progression of cardiovascular diseases.

Relationship of metabolism of reactive oxygen species with cytoplasmic male sterility in pepper (Capsicum annuum L.)

2012 ◽  
Vol 134 ◽  
pp. 232-236 ◽  
Author(s):  
Ming-Hua Deng ◽  
Jin-Fen Wen ◽  
Jin-Long Huo ◽  
Hai-Shan Zhu ◽  
Xiong-Ze Dai ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Capsicum Annuum ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Capsicum Annuum L ◽  
Relationship Of

Protective effect of (-)-epigallocatechin-3-gallate against cisplatin-induced ototoxicity

2014 ◽  
Vol 128 (4) ◽  
pp. 350-355
Author(s):  
S I Cho ◽  
J H Lee ◽  
J H Park ◽  
N Y Do
Keyword(s):  
Reactive Oxygen Species ◽  
Western Blot ◽  
Reactive Oxygen Species Production ◽  
Caspase 3 ◽  
Organ Of Corti ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Pre Treatment ◽  
Species Production ◽  
Cisplatin Therapy

AbstractObjective:Ototoxicity due to cisplatin therapy interferes with treatment and often forces a reduction in the dosage, duration and frequency of the cisplatin therapy. (-)-Epigallocatechin-3-gallate is known to have the highest antioxidant potency among all tea catechins. This study aimed to investigate the effect of (-)-epigallocatechin-3-gallate on cisplatin ototoxicity in an auditory cell line: House Ear Institute-Organ of Corti 1 cells.Methods:Cultured House Ear Institute-Organ of Corti 1 cells were exposed to cisplatin with or without pre-treatment with (-)-epigallocatechin-3-gallate. Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 staining was used to identify cells undergoing apoptosis. Western blot analysis was conducted to determine whether (-)-epigallocatechin-3-gallate inhibited cisplatin-induced caspase activation. Intracellular reactive oxygen species production was examined to investigate whether (-)-epigallocatechin-3-gallate was capable of scavenging cisplatin-induced reactive oxygen species accumulation.Results:Cell viability significantly increased in cells pre-treated with (-)-epigallocatechin-3-gallate compared with cells exposed to cisplatin alone. Cisplatin increased cleaved caspase-3 on Western blot analysis; however, pre-treatment with (-)-epigallocatechin-3-gallate inhibited the expression of caspase-3. (-)-Epigallocatechin-3-gallate attenuated reactive oxygen species production and apoptosis in House Ear Institute-Organ of Corti 1 cells.Conclusion:(-)-Epigallocatechin-3-gallate protected against cisplatin cytotoxicity through anti-apoptotic and anti-oxidative effects. Therefore, (-)-epigallocatechin-3-gallate could play a preventive role in cisplatin-induced ototoxicity.

scholarly journals Food-borne Lactiplantibacillus plantarum protect normal intestinal cells against inflammation by modulating reactive oxygen species and IL-23/IL-17 axis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Roberta Prete ◽  
Natalia Garcia-Gonzalez ◽  
Carla D. Di Mattia ◽  
Aldo Corsetti ◽  
Natalia Battista
Keyword(s):  
Reactive Oxygen Species ◽  
Inflammatory Diseases ◽  
Reactive Oxygen ◽  
Normal Mucosa ◽  
Protective Role ◽  
Intestinal Cells ◽  
Oxygen Species ◽  
Inflammatory Damage ◽  
Food Borne ◽  
Pre Treatment

Abstract Food-associated Lactiplantibacillus plantarum (Lpb. plantarum) strains, previously classified as Lactobacillus plantarum, are a promising strategy to face intestinal inflammatory diseases. Our study was aimed at clarifying the protective role of food-borne Lpb. plantarum against inflammatory damage by testing the scavenging microbial ability both in selected strains and in co-incubation with normal mucosa intestinal cells (NCM460). Here, we show that Lpb. plantarum endure high levels of induced oxidative stress through partially neutralizing reactive oxygen species (ROS), whereas they elicit their production when co-cultured with NCM460. Moreover, pre-treatment with food-borne Lpb. plantarum significantly reduce pro-inflammatory cytokines IL-17F and IL-23 levels in inflamed NCM460 cells. Our results suggest that food-vehicled Lpb. plantarum strains might reduce inflammatory response in intestinal cells by directly modulating local ROS production and by triggering the IL-23/IL-17 axis with future perspectives on health benefits in the gut derived by the consumption of functional foods enriched with selected strains.

scholarly journals Production of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinerea are preceded and depend on a burst of calcium

2013 ◽  
Vol 13 (1) ◽  
pp. 160 ◽  
Author(s):  
Emna Beneloujaephajri ◽  
Alex Costa ◽  
Floriane L’Haridon ◽  
Jean-Pierre Métraux ◽  
Matteo Binda
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Botrytis Cinerea ◽  
Induced Resistance ◽  
Reactive Oxygen ◽  
Oxygen Species
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