Overexpression of tomato WHIRLY protein enhances tolerance to drought stress and resistance to Pseudomonas solanacearum in transgenic tobacco

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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Effect of overexpression of citrus 9-cis-epoxycarotenoid dioxygenase 3 (CsNCED3) on the physiological response to drought stress in transgenic tobacco

Genetics and Molecular Research ◽

10.4238/gmr16019292 ◽

2017 ◽

Vol 16 (1) ◽

Cited By ~ 5

Author(s):

A.M. Pedrosa ◽

L.C. Cidade ◽

C.P.S. Martins ◽

A.F. Macedo ◽

D.M. Neves ◽

...

Keyword(s):

Drought Stress ◽

Transgenic Tobacco ◽

Physiological Response

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Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Genes ◽

10.3390/genes12111706 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1706

Author(s):

Mei Yang ◽

Xinhang Duan ◽

Zhaoyu Wang ◽

Hang Yin ◽

Junrui Zang ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Cold Stress ◽

Medicago Sativa ◽

Transgenic Tobacco ◽

Soluble Sugars ◽

Ros Scavenging ◽

Osmotic Homeostasis ◽

Voltage Dependent ◽

Stress Responsive Genes

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel VDAC gene was isolated and identified from alfalfa (Medicago sativa L.). MsVDAC localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of MsVDAC in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O2− content increased. Stress-responsive genes including LTP1, ERD10B and Hxk3 were upregulated in the transgenic plants under cold and drought stress. However, GAPC, CBL1, BI-1, Cu/ZnSOD and MnSOD were upregulated only in the transgenic tobacco plants under cold stress, and GAPC, CBL1, and BI-1 were downregulated under drought stress. These results suggest that MsVDAC provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via MsVDAC may be mainly due to osmotic homeostasis and stress-responsive genes.

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Overexpression of LcSABP, an Orthologous Gene for Salicylic Acid Binding Protein 2, Enhances Drought Stress Tolerance in Transgenic Tobacco

Frontiers in Plant Science ◽

10.3389/fpls.2019.00200 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 11

Author(s):

Qian Li ◽

Gang Wang ◽

Chunfeng Guan ◽

Dan Yang ◽

Yurong Wang ◽

...

Keyword(s):

Salicylic Acid ◽

Drought Stress ◽

Stress Tolerance ◽

Transgenic Tobacco ◽

Binding Protein ◽

Orthologous Gene ◽

Drought Stress Tolerance ◽

Acid Binding Protein

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Isoprene production in transgenic tobacco alters isoprenoid, non-structural carbohydrate and phenylpropanoid metabolism, and protects photosynthesis from drought stress

Plant Cell & Environment ◽

10.1111/pce.12350 ◽

2014 ◽

Vol 37 (8) ◽

pp. 1950-1964 ◽

Cited By ~ 39

Author(s):

MASSIMILIANO TATTINI ◽

VIOLETA VELIKOVA ◽

CLAUDIA VICKERS ◽

CECILIA BRUNETTI ◽

MARTINA DI FERDINANDO ◽

...

Keyword(s):

Drought Stress ◽

Transgenic Tobacco ◽

Phenylpropanoid Metabolism ◽

Structural Carbohydrate

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The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants

10.21203/rs.3.rs-204710/v1 ◽

2021 ◽

Author(s):

Alireza Abbasi ◽

Meysam Malekpour ◽

Sajjad Sobhanverdi

Keyword(s):

Drought Stress ◽

Transgenic Plants ◽

Transgenic Tobacco ◽

Dry Weight ◽

Conventional Polymerase Chain Reaction ◽

Specific Gene ◽

Severe Drought ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Reverse Transcription Pcr

Abstract Expansins are cell wall proteins that, due to changes in pH, causing the expansion of the cell walls. In this study, a previously gene construct designed based on a root-specific gene, AtEXPA18, was utilized to assess its potential roles on different morphological, physiological, and cellular levels of generated transgenic tobacco plants in response to moderate and severe drought stress. AtEXPA18 gene was successfully transferred to the tobacco plants through an agrobacterium-mediate transformation system. Upon obtaining the second generation, tobacco transgenic plants were confirmed by conventional polymerase chain reaction (PCR) technique alongside reverse transcription PCR (RT-PCR) using specific primers. Under drought stress, the transgenic lines showed remarkable growth and significantly improved based on morphological traits such as height and stem diameter, leaf area, leaf number, root dry weight, and Abscisic acid (ABA) levels of leaves compared control plants. As a result, the Cytokinin content of transgenic plants has increased under severe stress levels. Notably, the area's expansion for abaxial epidermal cells under the microscope confirmed in transgene cells compared with the -transgene cells. These results, altogether, could support the AtEXPA18 gene implication in cell expansion and improving tolerance capacity of transgenic crops under drought stress.

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Kandelia candel Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco

International Journal of Molecular Sciences ◽

10.3390/ijms21093335 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3335 ◽

Cited By ~ 1

Author(s):

Xiaoshu Jing ◽

Jun Yao ◽

Xujun Ma ◽

Yanli Zhang ◽

Yuanling Sun ◽

...

Keyword(s):

Drought Stress ◽

Transgenic Plants ◽

Drought Tolerance ◽

Osmotic Stress ◽

Transgenic Tobacco ◽

Stomatal Closure ◽

H2o2 Production ◽

Kandelia Candel ◽

Ros Scavenging ◽

Thioredoxin F

Water deficit caused by osmotic stress and drought limits crop yield and tree growth worldwide. Screening and identifying candidate genes from stress-resistant species are a genetic engineering strategy to increase drought resistance. In this study, an increased concentration of mannitol resulted in elevated expression of thioredoxin f (KcTrxf) in the nonsecretor mangrove species Kandelia candel. By means of amino acid sequence and phylogenetic analysis, the mangrove Trx was classified as an f-type thioredoxin. Subcellular localization showed that KcTrxf localizes to chloroplasts. Enzymatic activity characterization revealed that KcTrxf recombinant protein possesses the disulfide reductase function. KcTrxf overexpression contributes to osmotic and drought tolerance in tobacco in terms of fresh weight, root length, malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) production. KcTrxf was shown to reduce the stomatal aperture by enhancing K+ efflux in guard cells, which increased the water-retaining capacity in leaves under drought conditions. Notably, the abscisic acid (ABA) sensitivity was increased in KcTrxf-transgenic tobacco, which benefits plants exposed to drought by reducing water loss by promoting stomatal closure. KcTrxf-transgenic plants limited drought-induced H2O2 in leaves, which could reduce lipid peroxidation and retain the membrane integrity. Additionally, glutathione (GSH) contributing to reactive oxygen species (ROS) scavenging and transgenic plants are more efficient at regenerating GSH from oxidized glutathione (GSSG) under conditions of drought stress. Notably, KcTrxf-transgenic plants had increased glucose and fructose contents under drought stress conditions, presumably resulting from KcTrxf-promoted starch degradation under water stress. We conclude that KcTrxf contributes to drought tolerance by increasing the water status, by enhancing osmotic adjustment, and by maintaining ROS homeostasis in transgene plants.

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