Effects of polyamine depletion by α-difluoromethylornithine on in vitro and in vivo biological properties of 4T1 murine mammary cancer cells

2006 ◽  
Vol 105 (1) ◽  
pp. 29-36 ◽  
Author(s):  
John Yoonkeun Jun ◽  
James W. Griffith ◽  
Richard Bruggeman ◽  
Sharlene Washington ◽  
Laurence M. Demers ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mammary Cancer ◽  
Biological Properties ◽  
Mammary Cancer Cells

Effects of polyamine depletion by α-difluoromethylornithine on in vitro and in vivo biological properties of 4T1 murine mammary cancer cells

2007 ◽  
Vol 107 (1) ◽  
pp. 33-40 ◽  
Author(s):  
John Yoonkeun Jun ◽  
James W. Griffith ◽  
Richard Bruggeman ◽  
Sharlene Washington ◽  
Laurence M. Demers ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mammary Cancer ◽  
Biological Properties ◽  
Mammary Cancer Cells

scholarly journals IRF-1 expression induces apoptosis and inhibits tumor growth in mouse mammary cancer cells in vitro and in vivo

Oncogene ◽  
2004 ◽  
Vol 23 (5) ◽  
pp. 1125-1135 ◽  
Author(s):  
Peter K M Kim ◽  
Michaele Armstrong ◽  
Ye Liu ◽  
Peng Yan ◽  
Brian Bucher ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Cancer Cells ◽  
Mammary Cancer ◽  
Mammary Cancer Cells

A novel class of ruthenium-based photosensitizers effectively kills in vitro cancer cells and in vivo tumors

2015 ◽  
Vol 14 (11) ◽  
pp. 2014-2023 ◽  
Author(s):  
Jamie Fong ◽  
Kamola Kasimova ◽  
Yaxal Arenas ◽  
Pavel Kaspler ◽  
Savo Lazic ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Biological Properties

The photo-physical and photo-biological properties of two small (<2 kDa), novel Ru(ii) photosensitizers (PSs) referred to as TLD1411 and TLD1433 are presented.

T1998 The Muc4 Membrane-Bound Mucin Alters In Vitro and In Vivo Biological Properties of Human Pancreatic Cancer Cells

2009 ◽  
Vol 136 (5) ◽  
pp. A-617
Author(s):  
Nicolas Jonckheere ◽  
Nicolas Skrypek ◽  
Nathalie Saint-Laurent ◽  
Christiane Susini ◽  
Isabelle VAN SEUNINGEN
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Biological Properties ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells ◽  
Membrane Bound

scholarly journals Tumor Microenvironment Regulates Metastasis and Metastasis Genes of Mouse MMTV-PymT Mammary Cancer Cells In Vivo

2013 ◽  
Vol 51 (4) ◽  
pp. 868-881 ◽  
Author(s):  
J. L. Werbeck ◽  
N. K. Thudi ◽  
C. K. Martin ◽  
C. Premanandan ◽  
L. Yu ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Cancer Cells ◽  
Mammary Cancer ◽  
Mammary Cancer Cells

scholarly journals Toxoplasma gondii destroys Her2/Neu-expressing mammary cancer cells in vitro using a continuous feed medium approach

2020 ◽  
Vol 14 (10) ◽  
pp. 1204-1209
Author(s):  
Esra Atalay Şahar ◽  
Mert Döşkaya ◽  
Muhammet Karakavuk ◽  
Hüseyin Can ◽  
Aytül Gül ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Cancer Cells ◽  
Cell Lines ◽  
Mammary Cancer ◽  
Mammary Cancer Cell ◽  
Continuous Feed ◽  
Feed Medium ◽  
The Moment ◽  
Mammary Cancer Cells

Introduction: Toxoplasma gondii is an opportunistic protozoan and can be grown using several human cell lines. Breast cancer is the second leading cause of cancer death in women. Her2/Neu-expressing mammary cancer cell lines called TUBO can be grown in vitro. In recent years, protozoan parasites have become popular means of use in cancer therapy research. In this study, we analyzed whether T. gondii tachyzoites can destroy TUBO cells using a novel continuous feed medium approach. Methodology: Two sets of flasks (each containing four groups) containing TUBO cells were inoculated with T. gondii Ankara strain tachyzoites. First set containing 5×106 TUBO cells were inoculated with TUBO-tachyzoite ratios of 1:2, 1:1, 2:1, and 4:1 and second set containing 1×106 TUBO cells were inoculated with TUBO-tachyzoite ratios of 10:1, 100:1, 1000:1, and 2000:1. Thereafter, culture supernatants were harvested at various days until TUBO cells were destroyed and tachyzoites were counted. Results: In the first and second sets of flasks, TUBO cells were destroyed between days 8 to 12 and 12 to 25, respectively. In addition, the amount of tachyzoites increased 7- 43 and 595 to 112500 times in the first and second set of flasks, respectively. Conclusions: These results show that T. gondii tachyzoites successfully destroy Her2/Neu-expressing mammary cancer cells using a continuous feed medium approach. Although this idea may be too premature for the moment, the approach defined herein may support future researchers investigating the relationship between cancer and parasites which can make important progress toward saving cancer patient lives.

scholarly journals Melatonin decreases in vitro viability and migration of spheres derived from CF41.Mg canine mammary carcinoma cells

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Consuelo Serrano ◽  
Sofía Guzmán ◽  
Jose Ignacio Arias ◽  
Cristian Gabriel Torres
Keyword(s):  
Cell Viability ◽  
Cancer Cells ◽  
Mammary Carcinoma ◽  
Mammary Cancer ◽  
Carcinoma Cells ◽  
Canine Mammary Carcinoma ◽  
Mammary Carcinoma Cells ◽  
And Migration ◽  
Mammary Cancer Cells

Abstract Background Mammary cancer is a common disease affecting female dogs, where approximately 50% of the cases are malignant. There is a subpopulation of cancer cells with stem cell-like features within the tumour microenvironment, which can form in vitro spheres, cell structures that grow in anchor-free conditions. This cell population shows resistance to conventional antitumor treatments explaining in part the recurrence of some type of cancers. It has been previously reported that spheres derived from CF41.Mg canine mammary carcinoma cells exhibit several stemness features. Melatonin has shown antitumor effects on cancer mammary cells; nevertheless, its effects have been poorly evaluated on canine mammary cancer stem-like cells. In this regard, it has described that melatonin decreases the expression of OCT-4 in CMT-U2229 mammary cancer cells, a transcription factor that participates in the modulation of self-renewal and drug resistance in cancer stem-like cells. The aim of this study was to compare the effects of melatonin on viability and migration of canine mammary carcinoma CF41.Mg-spheres, and CF41.Mg-parental cells. CF41.Mg cells were grown in DMEM high-glucose medium containing 10% bovine foetal serum. CF41.Mg-spheres were cultured in ultra-low attachment plates with serum-free DMEM/F12 containing several growth factors. Cell viability (MTS reduction) and migration (transwell) assays were conducted in presence of melatonin (0.01, 0.1 or 1 mM). Results Melatonin decreased cell viability at 1 mM (P < 0.05), with a significant reduction in spheres compared to parental cells at 24 and 48 h (P < 0.05). Cell migration was inhibited in response to non-cytotoxic concentration of melatonin (0.1 mM) (P < 0.05) in spheres and monolayer of cells, no significant differences were detected between both cell subtypes. Conclusions These results indicate that melatonin reduces viability and migration of CF41.Mg cells, where spheres exhibit greater sensitivity to the hormone. Thus, melatonin represents a valuable potential agent against mammary cancer cells, especially cancer stem-like cells.

scholarly journals Ganoderma tsugaeExtract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Han-Peng Kuo ◽  
Shih-Chung Hsu ◽  
Chien-Chih Ou ◽  
Jhy-Wei Li ◽  
Hsiu-Hsueh Tseng ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Common Species ◽  
Biological Properties ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Her2 Protein

Ganoderma, also known as Lingzhi or Reishi, has been used for medicinal purposes in Asian countries for centuries. It is a medicinal fungus with a variety of biological properties including immunomodulatory and antitumor activities. In this study, we investigated the molecular mechanisms by whichGanoderma tsugae(GT), one of the most common species ofGanoderma, inhibits the proliferation of HER2-overexpressing cancer cells. Here, we show that a quality assured extract of GT (GTE) inhibited the growth of HER2-overexpressing cancer cellsin vitroandin vivoand enhanced the growth-inhibitory effect of antitumor drugs (e.g., taxol and cisplatin) in these cells. We also demonstrate that GTE induced cell cycle arrest by interfering with the HER2/PI3K/Akt signaling pathway. Furthermore, GTE curtailed the expression of the HER2 protein by modulating the transcriptional activity of theHER2gene and the stability/degradation of the HER2 protein. In conclusion, this study suggests that GTE may be a useful adjuvant therapeutic agent in the treatment of cancer cells that highly express HER2.

scholarly journals The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture in vitro

2012 ◽  
Vol 8 (1) ◽  
pp. 35 ◽  
Author(s):  
Magdalena Król ◽  
Karol M Pawłowski ◽  
Katarzyna Szyszko ◽  
Henryk Maciejewski ◽  
Izabella Dolka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cancer Cells ◽  
Mammary Cancer ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Culture In Vitro ◽  
Canine Mammary Cancer ◽  
Carcinoma Associated Fibroblasts ◽  
Mammary Cancer Cells
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